公开(公告)号：US20070111220A1

公开(公告)日：2007-05-17

申请号：US11286621

申请日：2005-11-23

申请人： Laszlo Kish ,  Maria Dobozi-King ,  Ryland Young ,  Mosong Cheng ,  James Biard ,  Sergey Bezrukov

发明人： Laszlo Kish ,  Maria Dobozi-King ,  Ryland Young ,  Mosong Cheng ,  James Biard ,  Sergey Bezrukov

IPC分类号： C12Q1/68

CPC分类号： B82Y30/00 ,  B01L3/5088 ,  C12N7/00 ,  C12N2795/10311 ,  C12N2795/10322 ,  C12N2799/00 ,  C12Q1/04 ,  C12Q1/6825 ,  C12Q1/689 ,  G01N33/48707 ,  G01N33/54373 ,  G01N33/56911

摘要： The present invention provides a method for detecting bacteria and a nano-well device having one or more input/output connections about a gap and one or more bacteriophages at or about the gap that trigger a detectable electrical field fluctuation when the one or more bacteriophages contact a cognate target within a liquid sample.

摘要翻译： 本发明提供一种用于检测细菌的方法和具有一个或多个输入/输出连接的纳米孔装置，所述输入/输出连接围绕间隙和在间隙处或周围的一个或多个噬菌体，当一个或多个噬菌体接触时触发可检测的电场波动 液体样品中的同源靶标。

公开(公告)号：US20120252003A1

公开(公告)日：2012-10-04

申请号：US13075250

申请日：2011-03-30

申请人： Gabor Schmera ,  Laszlo Kish

发明人： Gabor Schmera ,  Laszlo Kish

IPC分类号： C12Q1/70 ,  C12M1/42

CPC分类号： G01N33/48735

摘要： Methods for detection and identification of bacteria within a sample include the step of inserting a pair of electrodes into the sample. A first impedance across the electrodes is established with a first AC voltage source having a first frequency. A phage is introduced into the sample, and impedance fluctuations that are caused by ion release by the bacteria due to the phage introduction are measured. The use of impedance fluctuations instead of voltage fluctuations to detect and identify bacteria minimizes 1/f noise effects and increases system sensitivity. To further increase system sensitivity by eliminating thermal noise, a second impedance across the electrodes can be established using a second AC voltage source at a second frequency. Second impedance fluctuations are cross-correlated to the first impedance fluctuations, and the cross-correlation results are analyzed to determine whether or not bacteria are present in the sample based on phage electrical activity.

摘要翻译： 用于检测和鉴定样品中的细菌的方法包括将一对电极插入样品中的步骤。 利用具有第一频率的第一AC电压源建立电极两端的第一阻抗。 样品中引入了噬菌体，测量了由于引入噬菌体而导致细菌离子释放引起的阻抗波动。 使用阻抗波动代替电压波动来检测和识别细菌可以最大限度地降低1 / f噪声影响并提高系统灵敏度。 为了通过消除热噪声来进一步提高系统灵敏度，可以使用第二频率的第二AC电压源来建立电极两端的第二阻抗。 第二阻抗波动与第一阻抗波动相互关联，并且分析互相关结果以基于噬菌体电活动来确定样品中是否存在细菌。

公开(公告)号：US09645101B2

公开(公告)日：2017-05-09

申请号：US13936631

申请日：2013-07-08

申请人： Gabor Schmera ,  Laszlo Kish

发明人： Gabor Schmera ,  Laszlo Kish

IPC分类号： G01N27/02 ,  G01N33/569 ,  G01N33/487

CPC分类号： G01N27/021 ,  G01N33/48735 ,  G01N33/56911

摘要： Methods for detection and identification of bacteria within a sample include the step of inserting a pair of electrodes into the sample. A first impedance across the electrodes is established with a first AC voltage source having a first frequency. A phage is introduced into the sample, and impedance fluctuations that are caused by ion release by the bacteria due to the phage introduction are measured. The use of impedance fluctuations instead of voltage fluctuations to detect and identify bacteria minimizes 1/f noise effects and increases system sensitivity. To further increase system sensitivity by eliminating thermal noise, a second impedance across the electrodes can be established using a second AC voltage source at a second frequency. Second impedance fluctuations are cross-correlated to the first impedance fluctuations, and the cross-correlation results are analyzed to determine whether or not bacteria are present in the sample based on phage electrical activity.

公开(公告)号：US20130295556A1

公开(公告)日：2013-11-07

申请号：US13936631

申请日：2013-07-08

申请人： Gabor Schmera ,  Laszlo Kish

发明人： Gabor Schmera ,  Laszlo Kish

IPC分类号： G01N27/02

CPC分类号： G01N27/021 ,  G01N33/48735 ,  G01N33/56911

摘要： Methods for detection and identification of bacteria within a sample include the step of inserting a pair of electrodes into the sample. A first impedance across the electrodes is established with a first AC voltage source having a first frequency. A phage is introduced into the sample, and impedance fluctuations that are caused by ion release by the bacteria due to the phage introduction are measured. The use of impedance fluctuations instead of voltage fluctuations to detect and identify bacteria minimizes 1/f noise effects and increases system sensitivity. To further increase system sensitivity by eliminating thermal noise, a second impedance across the electrodes can be established using a second AC voltage source at a second frequency. Second impedance fluctuations are cross-correlated to the first impedance fluctuations, and the cross-correlation results are analyzed to determine whether or not bacteria are present in the sample based on phage electrical activity.

摘要翻译： 用于检测和鉴定样品中的细菌的方法包括将一对电极插入样品中的步骤。 利用具有第一频率的第一AC电压源建立电极两端的第一阻抗。 样品中引入了噬菌体，测量了由于引入噬菌体而导致细菌离子释放引起的阻抗波动。 使用阻抗波动代替电压波动来检测和识别细菌可以最大限度地降低1 / f噪声影响并提高系统灵敏度。 为了通过消除热噪声来进一步提高系统灵敏度，可以使用第二频率的第二AC电压源来建立电极两端的第二阻抗。 第二阻抗波动与第一阻抗波动相互关联，并且分析互相关结果以基于噬菌体电活动来确定样品中是否存在细菌。