公开(公告)号：US11754477B2

公开(公告)日：2023-09-12

申请号：US17398083

申请日：2021-08-10

Applicant: Leica Mikrosysteme GmbH

Inventor： Paul Wurzinger ,  Rainer Wogritsch ,  Ricardo Vizcaya Benavides ,  Julia König

IPC: G01N1/42

CPC classification number: G01N1/42

Abstract: A high pressure freezing cartridge (300, 400, 500) for use in vitrification of a biological sample (10) is provided, the cartridge (300, 400, 500) being adapted to fixedly hold a sample chamber (216, 218, 220) at a sample chamber position (320, 420, 520) in the cartridge (300, 400, 500), a refrigerant channel arrangement comprising at least one refrigerant channel (208, 308, 408, 462, 464, 466, 468, 508, 562, 564, 566, 569) being provided in the cartridge (300, 400, 500) and extending from a surface of the cartridge (300, 400, 500) to the sample chamber position, and the cartridge (300, 400, 500) comprising a baffle structure (350, 450, 550, 571) at a baffle position being adapted to interact with a refrigerant stream in the refrigerant channel arrangement before the refrigerant of the refrigerant stream reaches the sample chamber position in the cartridge (300, 400, 500). A corresponding method in which the high pressure freezing cartridge (300, 400, 500) is used is also part of the present disclosure.

公开(公告)号：US20230386779A1

公开(公告)日：2023-11-30

申请号：US18320212

申请日：2023-05-19

Applicant: Leica Mikrosysteme GMBH ,  Leica Microsystems CMS GmbH

Inventor： Thomas Pfeifer ,  Heinz Plank ,  Miriam Weller ,  Konrad Winkler ,  Rainer Wogritsch

IPC: H01J37/20

CPC classification number: H01J37/20 ,  H01J2237/204 ,  H01J2237/2007

Abstract: A sample holder includes a main component comprising a first surface area, and a retaining mechanism comprising a holding component. The retaining mechanism is configured to retain a sample between the holding component of the retaining mechanism and the first surface area in a closed state of the retaining mechanism. The retaining mechanism is further configured to enter an opened state upon mechanical actuation of the retaining mechanism. The opened state allows insertion and removal of the sample. The retaining mechanism is further configured to revert to the closed state once the mechanical actuation is removed.

公开(公告)号：US10809211B2

公开(公告)日：2020-10-20

申请号：US16327835

申请日：2017-07-13

Applicant: LEICA MIKROSYSTEME GMBH

Inventor： Rainer Wogritsch ,  Cveta Tomova ,  Paul Wurzinger ,  Heinz Plank ,  Siegfried Tanki

IPC: G01N23/20 ,  G01N23/20033 ,  G01N1/42 ,  G01N23/20025 ,  G01N23/20041

Abstract: A modular specimen holder (10′) for high pressure freezing and/or X-ray crystallography of a specimen has a specimen holding element (100′) and an extension element (200′) connectable with and separable from each other; the specimen holding element (100′) including a tubule (120′) adapted to hold the specimen and a base element (110′) adapted to hold the tubule (120′), wherein a distance from a bottom of the base element (110′) to a top of the tubule (120′) is a first distance (d1); the extension element (200′) being connectable with the base element (110′), wherein, when the extension element (200′) and the base element (110′) are connected with each other, a second distance (d2) from a bottom of the extension element (200′) to the top of the tubule (120′) is larger than the first distance (d1).

公开(公告)号：US11828692B2

公开(公告)日：2023-11-28

申请号：US17460383

申请日：2021-08-30

Applicant: Leica Mikrosysteme GmbH

Inventor： Rainer Wogritsch ,  Paul Wurzinger

IPC: G01N1/42 ,  F25D3/10 ,  C12M1/00

CPC classification number: G01N1/42 ,  F25D3/10 ,  C12M41/14 ,  F25D2400/30

Abstract: A device for preparing a microscopic sample for a high-pressure freezing process in which the sample is provided using an arrangement having a middle plate of a high-pressure freezing cartridge and an incubation chamber, the middle plate being attached to and detachable from the incubation chamber by effecting a relative movement between the middle plate and the incubation chamber, and the sample being provided on an enclosing element which is fitted into an opening of the middle plate, wherein the device includes an engagement structure adapted to engage with the middle plate and to restrict a movement of the middle plate while the incubation chamber is moved, such as to effect the relative movement between the middle plate and the incubation chamber and to thereby detach the middle plate from the incubation chamber.

公开(公告)号：US09097632B2

公开(公告)日：2015-08-04

申请号：US13778720

申请日：2013-02-27

Applicant: Leica Mikrosysteme GmbH

Inventor： Reinhard Lihl ,  Rainer Wogritsch ,  Heinz Plank

IPC: F25D25/00 ,  F25B19/00 ,  F25D23/00 ,  F24F3/16 ,  G01N1/42 ,  B29C45/00 ,  G01Q30/10 ,  G02B7/00 ,  G21K7/00 ,  B29K83/00

CPC classification number: G01N1/42 ,  B29C45/0053 ,  B29C2045/0075 ,  B29K2083/005 ,  G01Q30/10 ,  G02B7/007 ,  G21K7/00

Abstract: In a device (1) for rapid pressure-freezing an aqueous sample (3), such as a biological specimen, a pressurized cooling medium can be fed into a high-pressure chamber (11) into which a sample holder (30) containing a sample (3) is inserted and which is sealed with a pressure-tight seal, to the location of the sample holder (30) held therein. The high-pressure chamber (11) comprises a viewing window structure (2) with a pressure-tight window (20), through which light can be directed from the outside onto the sample (3) located in the sample holder (30). The window (20) can comprise a transparent window element made of a high-pressure-resistant material, wherein the window element (20) is held by a pressure- and temperature-resistant window bearing provided in the high-pressure chamber.

Abstract translation: 在用于快速冷冻含水样品（3）（例如生物样品）的装置（1）中，加压冷却介质可以被供给到高压室（11）中，其中含有 将样品（3）插入并且用耐压密封件密封到保持在其中的样品保持器（30）的位置。 高压室（11）包括具有耐压窗（20）的观察窗结构（2），光可从外部引导到位于样品架（30）中的样品（3）上。 窗口（20）可以包括由耐高压材料制成的透明窗口元件，其中窗口元件（20）由设置在高压室中的耐压耐温窗口轴承保持。

公开(公告)号：US11940362B2

公开(公告)日：2024-03-26

申请号：US17460380

申请日：2021-08-30

Applicant: Leica Mikrosysteme GmbH

Inventor： Rainer Wogritsch ,  Paul Wurzinger ,  Joose Kreutzer

IPC: G01N1/42 ,  F25D3/10 ,  C12M1/00

CPC classification number: G01N1/42 ,  F25D3/10 ,  C12M41/14 ,  F25D2400/30

Abstract: A method including one or more steps of a procedure for preparing a microscopic sample for a high-pressure freezing process is provided, wherein the sample is provided using an arrangement comprising a middle plate of a high-pressure freezing cartridge and an incubation chamber, wherein the middle plate is attached to a lower surface of a bottom of the incubation chamber by an adhesive and detachable from the incubation chamber by effecting a relative movement between the middle plate and the incubation chamber, wherein the sample is provided on an enclosing element which is fitted into an opening of the middle plate, and wherein, when the sample is to be subjected to the high-pressure freezing process, the relative movement between the middle plate and the incubation chamber is effected, thereby detaching the middle plate with the sample from the incubation chamber. Means to implement the method are also part of the present disclosure.