公开(公告)号：US12123047B2

公开(公告)日：2024-10-22

申请号：US17839586

申请日：2022-06-14

Applicant: Leica Mikrosysteme GmbH

Inventor： Julia König ,  Paul Wurzinger

IPC: C12Q1/02 ,  G01N1/28 ,  G01N1/42 ,  G01N21/64 ,  G01N23/04 ,  G01N23/06

CPC classification number: C12Q1/02 ,  G01N1/286 ,  G01N1/42 ,  G01N21/6458 ,  G01N23/04 ,  G01N23/06 ,  G01N2001/2873 ,  G01N2223/04 ,  G01N2223/418

Abstract: A method (400) for microscopic examination of a sample (1) includes applying (410) the sample (1) to a sample holder (10) having a transparent carrier material, capturing (420) a first image (210, 220) of the sample (1) applied to the sample holder (10) using a first light-microscopy method, cryofixing, freeze-substituting, and subsequently infiltrating and embedding (430) the sample (1) together with the sample holder (10) with an embedding medium (20) in an embedding mold (90, 100), curing (440) the embedding medium (20), removing the sample (1) from the embedding mold (90, 100) together with the embedding medium (20) and the sample holder (10), capturing (450) a second image (230) of the sample (1) embedded in the cured embedding medium (20) using a second light-microscopy method, wherein at least partially identical regions of the sample (1) are captured in the first and second images, and identifying (460) at least one portion of the first image (210, 220) and one portion of the second image (230) which show identical regions of the sample (1).

公开(公告)号：US11940362B2

公开(公告)日：2024-03-26

申请号：US17460380

申请日：2021-08-30

Applicant: Leica Mikrosysteme GmbH

Inventor： Rainer Wogritsch ,  Paul Wurzinger ,  Joose Kreutzer

IPC: G01N1/42 ,  F25D3/10 ,  C12M1/00

CPC classification number: G01N1/42 ,  F25D3/10 ,  C12M41/14 ,  F25D2400/30

Abstract: A method including one or more steps of a procedure for preparing a microscopic sample for a high-pressure freezing process is provided, wherein the sample is provided using an arrangement comprising a middle plate of a high-pressure freezing cartridge and an incubation chamber, wherein the middle plate is attached to a lower surface of a bottom of the incubation chamber by an adhesive and detachable from the incubation chamber by effecting a relative movement between the middle plate and the incubation chamber, wherein the sample is provided on an enclosing element which is fitted into an opening of the middle plate, and wherein, when the sample is to be subjected to the high-pressure freezing process, the relative movement between the middle plate and the incubation chamber is effected, thereby detaching the middle plate with the sample from the incubation chamber. Means to implement the method are also part of the present disclosure.

公开(公告)号：US20230341298A1

公开(公告)日：2023-10-26

申请号：US18299759

申请日：2023-04-13

Applicant: LEICA MIKROSYSTEME GMBH

Inventor： Matthias Katzengruber ,  Robert Ranner ,  Paul Wurzinger

IPC: G01N1/06 ,  G01N1/28

CPC classification number: G01N1/06 ,  G01N1/286 ,  G01N2001/061 ,  G01N2001/065 ,  G01N2001/068 ,  G01N2001/2873

Abstract: A microtome system for cutting sections from a specimen includes a knife including a knife edge configured to cut a section from the specimen, a knife holder, a specimen holder, an illumination, a first actor, a detector, and a controller. The knife holder and specimen holder are configured to be relatively moveable in a cutting direction. The first actor is configured to cause a rotation of the knife holder or specimen holder about an axis. The illumination is configured to illuminate a gap between a front face of the specimen and the knife edge to generate a light gap. The detector is configured to detect a geometric feature of the light gap. The controller is configured to automatically align, or provide indications to manually align, the knife edge with the front face, by controlling the first actor depending on the detected geometric feature.

公开(公告)号：US11754477B2

公开(公告)日：2023-09-12

申请号：US17398083

申请日：2021-08-10

Applicant: Leica Mikrosysteme GmbH

Inventor： Paul Wurzinger ,  Rainer Wogritsch ,  Ricardo Vizcaya Benavides ,  Julia König

IPC: G01N1/42

CPC classification number: G01N1/42

Abstract: A high pressure freezing cartridge (300, 400, 500) for use in vitrification of a biological sample (10) is provided, the cartridge (300, 400, 500) being adapted to fixedly hold a sample chamber (216, 218, 220) at a sample chamber position (320, 420, 520) in the cartridge (300, 400, 500), a refrigerant channel arrangement comprising at least one refrigerant channel (208, 308, 408, 462, 464, 466, 468, 508, 562, 564, 566, 569) being provided in the cartridge (300, 400, 500) and extending from a surface of the cartridge (300, 400, 500) to the sample chamber position, and the cartridge (300, 400, 500) comprising a baffle structure (350, 450, 550, 571) at a baffle position being adapted to interact with a refrigerant stream in the refrigerant channel arrangement before the refrigerant of the refrigerant stream reaches the sample chamber position in the cartridge (300, 400, 500). A corresponding method in which the high pressure freezing cartridge (300, 400, 500) is used is also part of the present disclosure.

公开(公告)号：US11828692B2

公开(公告)日：2023-11-28

申请号：US17460383

申请日：2021-08-30

Applicant: Leica Mikrosysteme GmbH

Inventor： Rainer Wogritsch ,  Paul Wurzinger

IPC: G01N1/42 ,  F25D3/10 ,  C12M1/00

CPC classification number: G01N1/42 ,  F25D3/10 ,  C12M41/14 ,  F25D2400/30

Abstract: A device for preparing a microscopic sample for a high-pressure freezing process in which the sample is provided using an arrangement having a middle plate of a high-pressure freezing cartridge and an incubation chamber, the middle plate being attached to and detachable from the incubation chamber by effecting a relative movement between the middle plate and the incubation chamber, and the sample being provided on an enclosing element which is fitted into an opening of the middle plate, wherein the device includes an engagement structure adapted to engage with the middle plate and to restrict a movement of the middle plate while the incubation chamber is moved, such as to effect the relative movement between the middle plate and the incubation chamber and to thereby detach the middle plate from the incubation chamber.

公开(公告)号：US10809211B2

公开(公告)日：2020-10-20

申请号：US16327835

申请日：2017-07-13

Applicant: LEICA MIKROSYSTEME GMBH

Inventor： Rainer Wogritsch ,  Cveta Tomova ,  Paul Wurzinger ,  Heinz Plank ,  Siegfried Tanki

IPC: G01N23/20 ,  G01N23/20033 ,  G01N1/42 ,  G01N23/20025 ,  G01N23/20041

Abstract: A modular specimen holder (10′) for high pressure freezing and/or X-ray crystallography of a specimen has a specimen holding element (100′) and an extension element (200′) connectable with and separable from each other; the specimen holding element (100′) including a tubule (120′) adapted to hold the specimen and a base element (110′) adapted to hold the tubule (120′), wherein a distance from a bottom of the base element (110′) to a top of the tubule (120′) is a first distance (d1); the extension element (200′) being connectable with the base element (110′), wherein, when the extension element (200′) and the base element (110′) are connected with each other, a second distance (d2) from a bottom of the extension element (200′) to the top of the tubule (120′) is larger than the first distance (d1).