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公开(公告)号：US20090264635A1
公开(公告)日：2009-10-22
申请号：US11909656
申请日：2006-03-27
CPC分类号： C12Q1/6848 , C12Q1/6844 , C12Q2525/186 , C12Q2521/107
摘要： The present invention concerns a system for isolating, depleting, and/or preventing the amplification of a targeted nucleic acid, such as mRNA or rRNA, from a sample comprising targeted and nontargeted nucleic acids.
摘要翻译： 本发明涉及用于从包含靶向和非靶向核酸的样品中分离，消耗和/或预防靶向核酸如mRNA或rRNA扩增的系统。
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公开(公告)号：US5783685A
公开(公告)日：1998-07-21
申请号：US620025
申请日：1996-03-21
申请人： Chander Bahl , Leopoldo Mendoza
发明人： Chander Bahl , Leopoldo Mendoza
CPC分类号： C07H21/00 , Y10S435/81
摘要： Methods of chemical synthesis of labeled nucleotide probes are described. The methods comprise providing a first target recognition moiety comprising a nucleotide sequence of at least about 15 nucleotide bases including at least one 5' end and 3' end. The first target recognition moiety is chemically altered to contain a carboxyl group. A second signal generating moiety comprising a hydroxyl group is provided. The carboxyl group of the first target recognition moiety is chemically reacted with hydroxyl group of the second signal generating moiety to chemically join the two moieties and produce said labeled nucleotide probe.
摘要翻译： 描述了标记核苷酸探针的化学合成方法。 所述方法包括提供包含至少约15个核苷酸碱基的核苷酸序列的第一靶识别部分，包括至少一个5'末端和3'末端。 第一个目标识别部分被化学改变以含有羧基。 提供了包含羟基的第二信号产生部分。 第一目标识别部分的羧基与第二信号产生部分的羟基化学反应以化学连接两个部分并产生所述标记的核苷酸探针。
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3.发明申请公开(公告)号：US20080221343A1
公开(公告)日：2008-09-11
申请号：US11787932
申请日：2007-04-18
IPC分类号： C07D303/02 , C07C241/00
CPC分类号： G01N33/582 , C07D303/02
摘要： Conjugationally extended hydrazine compositions of the formula (RR2)N(H)n(NH2)n, fluorescent hydrazone compositions of the formula (RR2)NN═C(R1R2), methods of the formation of hydrazones from the reaction of conjugationally extended hydrazines with conjugationally extended carbonyls and methods of their use in assays systems are described. Use of these conjugationally extended hydrazine and oxime compositions for direct calorimetric and fluorometric assays wherein a chromophore or the fluorophore is incorporated into the linker that is positioned between a reactive linking moiety and a biotin molecule. More specifically the linker comprises one molecule of a high affinity binding pair such as for example biotin of the biotin/avidin high affinity binding pair, connected to a spacer molecule such as for example a length of polyethyleneglycol followed by a pro-chromophoric, chromophoric, pro-fluorescent or fluorescent moiety connected to an amino-, thiol- or carbohydrate-reactive moiety such as for example succinimidyl, maleimido or aminoxy group respectively, that may covalently link to a biomolecule.
摘要翻译： 式（R 2 N）N（H）n（NH 2）n）的共轭延伸的肼组合物， 式（RR 2 N）NN-C（R 1 R 2 R 2）的荧光腙组合物，由反应形成腙的方法 描述了具有共轭延伸的羰基的共轭延伸的肼及其在测定系统中的用途。 使用这些共轭延伸的肼和肟组合物用于直接量热和荧光测定，其中发色团或荧光团被引入位于反应性连接部分和生物素分子之间的连接体中。 更具体地，接头包含一个高亲和力结合对分子，例如生物素/抗生物素蛋白高亲和力结合对的生物素，连接到间隔分子，例如长度的聚乙二醇，随后是发色团，发色团， 分别连接到可以共价连接到生物分子的氨基，硫醇或碳水化合物反应性部分的前荧光或荧光部分，例如琥珀酰亚胺基，马来酰亚胺基或氨氧基。
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公开(公告)号：US5538872A
公开(公告)日：1996-07-23
申请号：US302332
申请日：1994-09-08
申请人： Chander Bahl , Leopoldo Mendoza , Frank J. McMahon
发明人： Chander Bahl , Leopoldo Mendoza , Frank J. McMahon
CPC分类号： C07H21/00 , C12Q1/6813 , C12Q1/6816
摘要： The present invention provides labeled nucleotide probes and a procedure for synthesis of the labeled nucleotide probe by a coupling or joinder of two components of the probe, namely a target recognition sequence moiety and a signal generating moiety, through the use of a bridging complement that holds the two moieties in such a position as to allow formation of a sugar-phosphate chemical linkage between the 3' end of one moiety and the 5' end of the other moiety.
摘要翻译： 本发明提供了标记的核苷酸探针和用于通过使用衔接互补物（通过使用保持蛋白质）的桥接补体，通过探针的两个组分的偶联或并入来合成标记的核苷酸探针的方法，即靶识别序列部分和信号产生部分 这两个部分处于这样的位置，以允许在一个部分的3'末端和另一个部分的5'末端之间形成糖 - 磷酸酯化学键。
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5.发明授权公开(公告)号：US08541555B2
公开(公告)日：2013-09-24
申请号：US11787932
申请日：2007-04-18
IPC分类号： C07K16/00 , C07H21/04 , C07D235/02
CPC分类号： G01N33/582 , C07D303/02
摘要： Conjugationally extended hydrazine compositions of the formula (RR2)N(H)n(NH2)n, fluorescent hydrazone compositions of the formula (RR2)NN═C(R1R2), methods of the formation of hydrazones from the reaction of conjugationally extended hydrazines with conjugationally extended carbonyls and methods of their use in assays systems are described. Use of these conjugationally extended hydrazine and oxime compositions for direct calorimetric and fluorometric assays wherein a chromophore or the fluorophore is incorporated into the linker that is positioned between a reactive linking moiety and a biotin molecule. More specifically the linker comprises one molecule of a high affinity binding pair such as for example biotin of the biotin/avidin high affinity binding pair, connected to a spacer molecule such as for example a length of polyethyleneglycol followed by a pro-chromophoric, chromophoric, pro-fluorescent or fluorescent moiety connected to an amino-, thiol- or carbohydrate-reactive moiety such as for example succinimidyl, maleimido or aminoxy group respectively, that may covalently link to a biomolecule.
摘要翻译： 式（RR2）N（H）n（NH2）n的共轭延伸的肼组合物，式（RR2）NN = C（R1R2）的荧光腙组合物，从共轭延伸的肼与 描述了缀合扩展的羰基及其在测定系统中的用途。 使用这些共轭延伸的肼和肟组合物用于直接量热和荧光测定，其中发色团或荧光团被引入位于反应性连接部分和生物素分子之间的连接体中。 更具体地，接头包含一个高亲和力结合对分子，例如生物素/抗生物素蛋白高亲和力结合对的生物素，连接到间隔分子，例如长度的聚乙二醇，随后是发色团，发色团， 分别连接到可以共价连接到生物分子的氨基，硫醇或碳水化合物反应性部分的前荧光或荧光部分，例如琥珀酰亚胺基，马来酰亚胺基或氨氧基。
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6.发明授权High specific activity nucleotide probes having target recognition and signal generation moieties 失效具有目标识别和信号产生部分的高比活性核苷酸探针
公开(公告)号：US5521298A
公开(公告)日：1996-05-28
申请号：US142232
申请日：1993-10-25
申请人： Chander Bahl , Leopoldo Mendoza
发明人： Chander Bahl , Leopoldo Mendoza
CPC分类号： C07H21/00 , Y10S435/81
摘要： The probes of the invention are made up of a target recognition moiety and a signal generation moiety. In the first step of the preparatory method of the invention, the 5' or 3' terminus or both of the target recognition moiety is chemically altered to incorporate a reactive functionality which will allow chemical linkage of this target recognition moiety with one or both termini of the signal generation moiety of the probe. Suitable functionalities vary widely, but may, in general be described as those that allow chemical attachment of the two moieties either at the 5' or 3' end of the target recognition moiety. The two moieties should not detach from each other upon hybridization of the target moiety to the target analyte. Thus, when the probe is hybridized to target analyte, it will then carry with it, signal moiety, to label this complex, which label is then later detected.
摘要翻译： 本发明的探针由靶识别部分和信号产生部分组成。 在本发明的制备方法的第一步中，目标识别部分的5'或3'末端或两者被化学改变以引入反应性官能团，其将允许该目标识别部分与一个或两个末端的化学键合 探针的信号产生部分。 合适的功能性变化很大，但通常可以描述为允许两个部分在目标识别部分的5'或3'末端化学连接的功能。 当目标部分与目标分析物杂交时，两个部分不应相互分离。 因此，当探针与目标分析物杂交时，其随后携带信号部分，以标记该复合物，然后稍后检测该标记物。
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7.发明授权High specific activity nucleic acid probes having target recognition and signal generating moieties 失效具有靶识别和信号产生部分的高比活性核酸探针
公开(公告)号：US5324829A
公开(公告)日：1994-06-28
申请号：US285274
申请日：1988-12-16
申请人： Chander Bahl , Leopoldo Mendoza
发明人： Chander Bahl , Leopoldo Mendoza
CPC分类号： C07H21/00 , Y10S435/81
摘要： The probes of the invention are made up of a target recognition moiety and a signal generation moiety. In the first step of the preparatory method of the invention, the 5' or 3' terminus or both of the target recognition moiety is chemically altered to incorporate a reactive functionality which will allow chemical linkage of this target recognition moiety with one or both termini of the signal generation moiety of the probe. Suitable functionalities vary widely, but may, in general be described as those that allow chemical attachment of the two moieties either at the 5' or 3' end of the target recognition moiety. The two moieties should not detach from each other upon hybridization of the target moiety to the target analyte. Thus, when the probe is hybridized to target analyte, it will then carry with it, signal moiety, to label this complex, which label is then later detected.
摘要翻译： 本发明的探针由靶识别部分和信号产生部分组成。 在本发明的制备方法的第一步中，目标识别部分的5'或3'末端或两者被化学改变以引入反应性官能团，其将允许该目标识别部分与一个或两个末端的化学键合 探针的信号产生部分。 合适的功能性变化很大，但通常可以描述为允许两个部分在目标识别部分的5'或3'末端化学连接的功能。 当目标部分与目标分析物杂交时，两个部分不应相互分离。 因此，当探针与目标分析物杂交时，其随后携带信号部分，以标记该复合物，然后稍后检测该标记物。
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