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    Methods for the preparation of hybridomas producing lymphadenopathy-associated virus (LAV) GP110-specific monoclonal antibodies and methods for the purification of GP110 employing said monoclonal antibodies
    8.
    发明授权
    Methods for the preparation of hybridomas producing lymphadenopathy-associated virus (LAV) GP110-specific monoclonal antibodies and methods for the purification of GP110 employing said monoclonal antibodies 失效
    制备产生淋巴结病变相关病毒（LAV）的杂交瘤的方法GP110特异性单克隆抗体及使用所述单克隆抗体纯化GP110的方法

    公开(公告)号：US5610035A

    公开(公告)日：1997-03-11

    申请号：US313572

    申请日：1994-09-27

    申请人： Luc Montagnier ,  Bernard Krust ,  Solange Chamaret ,  Francois Clavel ,  Jean-Claude Chermann ,  Francoise Barre-Sinoussi

    发明人： Luc Montagnier ,  Bernard Krust ,  Solange Chamaret ,  Francois Clavel ,  Jean-Claude Chermann ,  Francoise Barre-Sinoussi

    IPC分类号： A23J1/00 ,  C07K16/00 ,  C12P21/04 ,  C12P21/08

    CPC分类号： A23J1/00 ,  C07K16/00

    摘要： A novel human retrovirus which is the aetiological agent of acquired immune deficiency syndrome (AIDS), designated lymphadenopathy-associated virus (LAV), was isolated from the lymph nodes of a patient suffering from generalized persistant lymphadenopathy. LAV displays a tropism for CD4.sup.+ lymphocytes, a Mg.sup.2+ -dependent reverse transcriptase (RT) activity, a density of 1.16-1.17 as determined by sucrose gradient centrifugation, a particle diameter of 139 nm, and a p25 antigen that is immunologically distinct from the HTLV-I/II p24 protein. Radioimmunoprecipitation analysis (RIPA) of .sup.35 S-cysteine-labeled viral or cellular lysates employing patient antisera resulted in the identification of a viral antigen having a molecular weight of 110 kDa. Endoglycosidase treatment of this antigen produces a protein with a molecular weight of 90 kDa. Methods are disclosed for the preparation of hybridomas producing LAV gp110-specific monoclonal antibodies (Mabs). Methods are also disclosed employing these MAbs in the purification of the LAV envelope glycoprotein, gp110.

    摘要翻译： 从患有广泛性持续性淋巴结病的患者的淋巴结中分离出一种新的人类逆转录病毒，其是获得性免疫缺陷综合征（AIDS）的病原体，称为淋巴结病伴相关病毒（LAV）。 LAV显示CD4 +淋巴细胞趋向性，Mg2 +依赖性逆转录酶（RT）活性，通过蔗糖梯度离心测定的密度为1.16-1.17，粒径为139nm，与HTLV免疫区别的p25抗原 -I / II p24蛋白。 使用患者抗血清的35S-半胱氨酸标记的病毒或细胞裂解物的放射免疫沉淀分析（RIPA）导致鉴定分子量为110kDa的病毒抗原。 该抗原的糖苷内切酶处理产生分子量为90kDa的蛋白质。 公开了制备产生LAV gp110特异性单克隆抗体（Mabs）的杂交瘤的方法。 还公开了使用这些MAb来纯化LAV包膜糖蛋白gp110的方法。