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公开(公告)号：US07323327B2
公开(公告)日：2008-01-29
申请号：US10469204
申请日：2002-02-22
申请人： Luppo Edens , Albertus Van Dijk , Philipp Krubasik , Kaj Albermann , Alexander Stock , Erik Kimpel , Sabine Klugbauer , Christian Wagner , Andreas Fritz , Wilk Von Gustedt , Oliver Heinrich , Dieter Maier , Fabio Spreafico , Ulrike Folkers , Sylvia Hopper , Wolfram Kemmner , Pamela Tan , Josephine Stiebler , Richard Albang
发明人： Luppo Edens , Albertus Van Dijk , Philipp Krubasik , Kaj Albermann , Alexander Stock , Erik Kimpel , Sabine Klugbauer , Christian Wagner , Andreas Fritz , Wilk Von Gustedt , Oliver Heinrich , Dieter Maier , Fabio Spreafico , Ulrike Folkers , Sylvia Hopper , Wolfram Kemmner , Pamela Tan , Josephine Stiebler , Richard Albang
CPC分类号： A23J3/16 , A23J3/343 , A23J3/344 , A23J3/346 , A23L2/02 , A23L2/84 , A23L33/18 , A23L33/40 , C07K2319/00 , C12H1/003 , C12N9/62 , C12Q1/37 , G01N33/56961 , G01N33/573 , G01N2333/38
摘要： The invention relates to newly identified gene sequences that encode novel proteases obtainable from Aspergillus niger. The invention features the full length gene sequence of the novel genes, their cDNA sequences as well as the full-length functional protein and fragments thereof. The invention also relates to methods of using these enzymes in industrial processes and methods of diagnosing fungal infections. Also included in the invention are cells transformed with DNA according to the invention and cells wherein a protease according to the invention is genetically modified to enhance or reduce its activity and/or level of expression.
摘要翻译： 本发明涉及新鉴定的编码从黑曲霉获得的新型蛋白酶的基因序列。 本发明的特征在于新基因的全长基因序列，其cDNA序列以及全长功能蛋白及其片段。 本发明还涉及在工业过程中使用这些酶的方法和诊断真菌感染的方法。 本发明还包括用根据本发明的DNA转化的细胞和其中根据本发明的蛋白酶被遗传修饰以增强或降低其活性和/或表达水平的细胞。
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公开(公告)号：US20050064403A1
公开(公告)日：2005-03-24
申请号：US10469204
申请日：2002-02-22
申请人： Luppo Edens , Dijk Alard , Philipp Krubasik , Kaj Albermann , Stock Alexander , Erik Kimpel , Sabine Klugbauer , Christian Wagner , Andreas Fritz , Gustedt Wilk , Oliver Heinrich , Dieter Maier , Fabio Spreafico , Ulrike Folkers , Sylvia Hopper , Wolfram Kemmner , Pamela Tan , Josephine Stiebler , Richard Albang
发明人： Luppo Edens , Dijk Alard , Philipp Krubasik , Kaj Albermann , Stock Alexander , Erik Kimpel , Sabine Klugbauer , Christian Wagner , Andreas Fritz , Gustedt Wilk , Oliver Heinrich , Dieter Maier , Fabio Spreafico , Ulrike Folkers , Sylvia Hopper , Wolfram Kemmner , Pamela Tan , Josephine Stiebler , Richard Albang
IPC分类号： A23J3/16 , A23J3/34 , A23L1/29 , A23L1/305 , A23L2/02 , A23L2/84 , C07K16/40 , C12H1/00 , C12N9/62 , C12N15/57 , C12N15/63 , C12Q1/37 , G01N33/569 , G01N33/573 , C12Q1/68 , C07H21/04 , C12N9/64
CPC分类号： A23J3/16 , A23J3/343 , A23J3/344 , A23J3/346 , A23L2/02 , A23L2/84 , A23L33/18 , A23L33/40 , C07K2319/00 , C12H1/003 , C12N9/62 , C12Q1/37 , G01N33/56961 , G01N33/573 , G01N2333/38
摘要： The invention relates to newly identified gene sequences that encode novel proteases obtainable from Aspergillus niger. The invention features the full length gene sequence of the novel genes, their cDNA sequences as well as the full-length functional protein and fragments thereof. The invention also relates to methods of using these enzymes in industrial processes and methods of diagnosing fungal infections. Also included in the invention are cells transformed with DNA according to the invention and cells wherein a protease according to the invention is genetically modified to enhance or reduce its activity and/or level of expression.
摘要翻译： 本发明涉及新鉴定的编码从黑曲霉获得的新型蛋白酶的基因序列。 本发明的特征在于新基因的全长基因序列，其cDNA序列以及全长功能蛋白及其片段。 本发明还涉及在工业过程中使用这些酶的方法和诊断真菌感染的方法。 本发明还包括用根据本发明的DNA转化的细胞和其中根据本发明的蛋白酶被遗传修饰以增强或降低其活性和/或表达水平的细胞。
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公开(公告)号：US20090275079A1
公开(公告)日：2009-11-05
申请号：US11976575
申请日：2007-10-25
申请人： Luppo Edens , Alard Van Albertus Dijk , Phillip Krubasik , Kaj Albermann , Alexander Stock , Erik Kimpel , Sabrine Klaubauer , Christian Wagner , Andreas Fritz , Wilk Von Gustedt , Oliver Henrich , Dieter Maier , Fabio Spreafico , Ulrike Folkers , Sylvia Hopper , Wolfram Kemmner , Pamela Tan , Josephine Stiebler , Ricahrd Albang
发明人： Luppo Edens , Alard Van Albertus Dijk , Phillip Krubasik , Kaj Albermann , Alexander Stock , Erik Kimpel , Sabrine Klaubauer , Christian Wagner , Andreas Fritz , Wilk Von Gustedt , Oliver Henrich , Dieter Maier , Fabio Spreafico , Ulrike Folkers , Sylvia Hopper , Wolfram Kemmner , Pamela Tan , Josephine Stiebler , Ricahrd Albang
CPC分类号： C12Q1/37 , C07K2319/00 , C12N9/62 , G01N33/56961 , G01N33/573 , G01N2333/38
摘要： The invention relates to newly identified gene sequences that encode novel proteases obtainable from Aspergillus niger. The invention features the full length gene sequence of the novel genes, their cDNA sequences as well as the full-length functional protein and fragments thereof. The invention also relates to methods of using these enzymes in industrial processes and methods of diagnosing fungal infections. Also included in the invention are cells transformed with DNA according to the invention and cells wherein a protease according to the invention is genetically modified to enhance or reduce its activity and/or level of expression.
摘要翻译： 本发明涉及新鉴定的编码从黑曲霉获得的新型蛋白酶的基因序列。 本发明的特征在于新基因的全长基因序列，其cDNA序列以及全长功能蛋白及其片段。 本发明还涉及在工业过程中使用这些酶的方法和诊断真菌感染的方法。 本发明还包括用根据本发明的DNA转化的细胞和其中根据本发明的蛋白酶被遗传修饰以增强或降低其活性和/或表达水平的细胞。
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公开(公告)号：US08216586B2
公开(公告)日：2012-07-10
申请号：US12453292
申请日：2009-05-06
申请人： Richard Albang , Andreas Fritz , Oliver Heinrich , Hilmar Ilgenfritz , Dieter Maier , Christian Wagner , Ulrike Folkers , Beatrix Gerhard , Fabio Spreafico , Lex De Boer , Roelf Bernhard Meima
发明人： Richard Albang , Andreas Fritz , Oliver Heinrich , Hilmar Ilgenfritz , Dieter Maier , Christian Wagner , Ulrike Folkers , Beatrix Gerhard , Fabio Spreafico , Lex De Boer , Roelf Bernhard Meima
CPC分类号： C12N9/20
摘要： The invention relates to a newly identified polynucleotide sequence comprising a gene that encodes a novel lipolytic enzyme from Aspergillus niger The invention features the full length nucleotide sequence of the novel gene, the cDNA sequence comprising the full length coding sequence of the novel lipolytic enzyme as well as the amino acid sequence of the full-length functional protein and functional equivalents thereof. The invention also relates to methods of using these enzymes in industrial processes and methods of diagnosing fungal infections. Also included in the invention are cells transformed with a polynucleotide according to the invention and cells wherein a lipolytic enzyme according to the invention is genetically modified to enhance its activity and/or level of expression.
摘要翻译： 本发明涉及新鉴定的多核苷酸序列，其包含编码来自黑曲霉的新型脂解酶的基因本发明的特征在于新基因的全长核苷酸序列，cDNA序列还包含新型脂肪分解酶的全长编码序列 作为全长功能性蛋白质的氨基酸序列及其功能等同物。 本发明还涉及在工业过程中使用这些酶的方法和诊断真菌感染的方法。 本发明还包括用根据本发明的多核苷酸转化的细胞和其中根据本发明的脂肪分解酶被遗传修饰以增强其活性和/或表达水平的细胞。
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公开(公告)号：US20090232936A1
公开(公告)日：2009-09-17
申请号：US12453292
申请日：2009-05-06
申请人： Richard Albang , Andreas Fritz , Oliver Heinrich , Hilmar Ilgenfritz , Dieter Maier , Christian Wagner , Ulrike Folkers , Beatrix Gerhard , Fabio Spreafico , Lex De Boer , Roelf Bernhard Meima
发明人： Richard Albang , Andreas Fritz , Oliver Heinrich , Hilmar Ilgenfritz , Dieter Maier , Christian Wagner , Ulrike Folkers , Beatrix Gerhard , Fabio Spreafico , Lex De Boer , Roelf Bernhard Meima
CPC分类号： C12N9/20
摘要： The invention relates to a newly identified polynucleotide sequence comprising a gene that encodes a novel lipolytic enzyme from Aspergillus niger The invention features the full length nucleotide sequence of the novel gene, the cDNA sequence comprising the full length coding sequence of the novel lipolytic enzyme as well as the amino acid sequence of the full-length functional protein and functional equivalents thereof. The invention also relates to methods of using these enzymes in industrial processes and methods of diagnosing fungal infections. Also included in the invention are cells transformed with a polynucleotide according to the invention and cells wherein a lipolytic enzyme according to the invention is genetically modified to enhance its activity and/or level of expression.
摘要翻译： 本发明涉及新鉴定的多核苷酸序列，其包含编码来自黑曲霉的新型脂解酶的基因本发明的特征在于新基因的全长核苷酸序列，cDNA序列还包含新型脂肪分解酶的全长编码序列 作为全长功能性蛋白质的氨基酸序列及其功能等同物。 本发明还涉及在工业过程中使用这些酶的方法和诊断真菌感染的方法。 本发明还包括用根据本发明的多核苷酸转化的细胞和其中根据本发明的脂肪分解酶被遗传修饰以增强其活性和/或表达水平的细胞。
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公开(公告)号：US07550280B2
公开(公告)日：2009-06-23
申请号：US10524983
申请日：2003-08-15
申请人： Richard Albang , Andreas Fritz , Oliver Heinrich , Hilmar Ilgenfritz , Dieter Maier , Christian Wagner , Ulrike Folkers , Beatrix Gerhard , Fabio Spreafico , Lex De Boer , Roelf Bernhard Meima
发明人： Richard Albang , Andreas Fritz , Oliver Heinrich , Hilmar Ilgenfritz , Dieter Maier , Christian Wagner , Ulrike Folkers , Beatrix Gerhard , Fabio Spreafico , Lex De Boer , Roelf Bernhard Meima
CPC分类号： C12N9/20
摘要： The invention relates to a newly identified polynucleotide sequence comprising a gene that encodes a novel lipolytic enzyme from Aspergillus niger. The invention features the full length nucleotide sequence of the novel gene, the cDNA sequence comprising the full length coding sequence of the novel lipolytic enzyme as well as the amino acid sequence of the full-length functional protein and functional equivalents thereof. The invention also relates to methods of using these enzymes in industrial processes and methods of diagnosing fungal infections. Also included in the invention are cells transformed with a polynucleotide according to the invention and cells wherein a lipolytic enzyme according to the invention is genetically modified to enhance its activity and/or level of expression.
摘要翻译： 本发明涉及新鉴定的多核苷酸序列，其包含编码来自黑曲霉的新型脂解酶的基因。 本发明的特征在于新基因的全长核苷酸序列，cDNA序列包含新型脂肪分解酶的全长编码序列以及全长功能蛋白的氨基酸序列及其功能等同物。 本发明还涉及在工业过程中使用这些酶的方法和诊断真菌感染的方法。 本发明还包括用根据本发明的多核苷酸转化的细胞和其中根据本发明的脂肪分解酶被遗传修饰以增强其活性和/或表达水平的细胞。
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公开(公告)号：US20060281080A1
公开(公告)日：2006-12-14
申请号：US10524983
申请日：2003-08-15
申请人： Richard Albang , Andreas Fritz , Oliver Heinrich , Hilmar Ilgenfritz , Dieter Maier , Christian Wagner , Ulrike Folkers , Beatrix Gerhard , Fabio Spreafico , Lex De Boer , Roelf Meima
发明人： Richard Albang , Andreas Fritz , Oliver Heinrich , Hilmar Ilgenfritz , Dieter Maier , Christian Wagner , Ulrike Folkers , Beatrix Gerhard , Fabio Spreafico , Lex De Boer , Roelf Meima
CPC分类号： C12N9/20
摘要： The invention relates to a newly identified polynucleotide sequence comprising a gene that encodes a novel lipolytic enzyme from Aspergillus niger. The invention features the full length nucleotide sequence of the novel gene, the cDNA sequence comprising the full length coding sequence of the novel lipolytic enzyme as well as the amino acid sequence of the full-length functional protein and functional equivalents thereof. The invention also relates to methods of using these enzymes in industrial processes and methods of diagnosing fungal infections. Also included in the invention are cells transformed with a polynucleotide according to the invention and cells wherein a lipolytic enzyme according to the invention is genetically modified to enhance its activity and/or level of expression.
摘要翻译： 本发明涉及新鉴定的多核苷酸序列，其包含编码来自黑曲霉的新型脂解酶的基因。 本发明的特征在于新基因的全长核苷酸序列，cDNA序列包含新型脂肪分解酶的全长编码序列以及全长功能蛋白的氨基酸序列及其功能等同物。 本发明还涉及在工业过程中使用这些酶的方法和诊断真菌感染的方法。 本发明还包括用根据本发明的多核苷酸转化的细胞和其中根据本发明的脂肪分解酶被遗传修饰以增强其活性和/或表达水平的细胞。
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公开(公告)号：US20050032059A1
公开(公告)日：2005-02-10
申请号：US10486868
申请日：2002-08-02
IPC分类号： C12N15/09 , A21D8/04 , C07K14/38 , C07K16/14 , C07K16/40 , C07K19/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/30 , C12N15/56 , C12Q1/68 , C07H21/04 , C12N1/16 , C12N15/74
CPC分类号： C12N9/242 , C07K2319/00
摘要： The invention relates to newly identified polynucleotide sequences comprising genes that encode novel amylases isolated from Aspergillus niger. The invention features the full length nucleotide sequences of the novel genes, the cDNA sequences comprising the full length coding sequence of the novel amylases as well as the amino acid sequence of the full-length functional proteins and functional equivalents thereof. The invention also relates to methods of using these enzymes in industrial processes and methods of diagnosing fungal infections. Also included in the invention are cells transformed with a polynucleotide according to the invention and cells wherein an amylase according to the invention is genetically modified to enhance or reduce its activity and/or level of expression.
摘要翻译： 本发明涉及新鉴定的多核苷酸序列，其包含编码从黑曲霉分离的新型淀粉酶的基因。 本发明的特征在于新基因的全长核苷酸序列，cDNA序列包含新型淀粉酶的全长编码序列以及全长功能蛋白质的氨基酸序列及其功能等同物。 本发明还涉及在工业过程中使用这些酶的方法和诊断真菌感染的方法。 本发明还包括用根据本发明的多核苷酸转化的细胞和其中根据本发明的淀粉酶被遗传修饰以增强或降低其活性和/或表达水平的细胞。
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