公开(公告)号：US20170320039A1

公开(公告)日：2017-11-09

申请号：US15586246

申请日：2017-05-03

申请人： Massachusetts Institute of Technology

发明人： Ruixuan Gao ,  Ishan Gupta ,  Edward S. Boyden

IPC分类号： B01J19/10 ,  B82Y40/00

CPC分类号： B01J19/10 ,  B82Y40/00 ,  C01B33/02

摘要： A method for synthesizing nanoparticles by sonofragmentation includes dispersing ultra-thin substrate units in a solvent chosen for suitability for sonofragmentation of the substrate, forming a suspension; ultrasonicating the suspension for a length of time sufficient to fragment the substrate into nanoparticles that are dispersed in the solvent; and evaporating the solvent. Solvent exchange with a second solvent may be performed. The synthesized nanoparticles are highly crystalline and monodispersed. The surface of the synthesized nanoparticles may be functionalized by choosing the solvents according to chemistry related to the intended surface functionalization of the synthesized nanoparticles, by adding surfactants to one or more of the solvents, and/or by performing ligand exchange or chemical modification to replace surface-bonded solvent or surfactant molecules with other functional groups to produce nanoparticles having the desired surface functionalization.

公开(公告)号：US20210196856A1

公开(公告)日：2021-07-01

申请号：US17144551

申请日：2021-01-08

申请人： Massachusetts Institute of Technology

发明人： Edward Stuart Boyden ,  Nikita Obidin ,  Ruixuan Gao ,  Linyi Gao

IPC分类号： A61L27/16 ,  G01N1/31 ,  C08F220/56 ,  C08L33/26 ,  A61L27/56

摘要： The invention provides a method for preparing an expanded cell or tissue sample suitable for microscopic analysis. Expanding the sample can be achieved by binding, e.g., anchoring, key biomolecules to a DMAA-TF polymer network and swelling, or expanding, the polymer network, thereby moving the biomolecules apart as further described herein. As the biomolecules are anchored to the polymer network isotropic expansion of the polymer network retains the spatial orientation of the biomolecules resulting in an expanded, or enlarged, sample.

公开(公告)号：US20190256633A1

公开(公告)日：2019-08-22

申请号：US16267849

申请日：2019-02-05

申请人： Massachusetts Institute of Technology

发明人： Ruixuan Gao ,  Linyi Gao ,  Chih-Chieh Yu ,  Edward Stuart Boyden

IPC分类号： C08F220/30 ,  C12Q1/6834 ,  C12Q1/6841 ,  C08F220/34 ,  C08G65/26

摘要： The invention encompasses hydrogels, monomer precursors of the hydrogels, methods for the preparation thereof, and methods of use therefor. The linking of monomers can take place using non-radical, bioorthogonal reactions such as copper-free click-chemistry.

公开(公告)号：US20220126257A1

公开(公告)日：2022-04-28

申请号：US17464651

申请日：2021-09-01

申请人： Massachusetts Institute of Technology

发明人： Ruixuan Gao ,  Ishan Gupta ,  Edward S. Boyden

IPC分类号： B01J19/10 ,  B82Y40/00 ,  C01B33/02

摘要： A method for synthesizing nanoparticles by sonofragmentation includes dispersing ultra-thin substrate units in a solvent chosen for suitability for sonofragmentation of the substrate, forming a suspension; ultrasonicating the suspension for a length of time sufficient to fragment the substrate into nanoparticles that are dispersed in the solvent; and evaporating the solvent. Solvent exchange with a second solvent may be performed. The synthesized nanoparticles are highly crystalline and monodispersed. The surface of the synthesized nanoparticles may be functionalized by choosing the solvents according to chemistry related to the intended surface functionalization of the synthesized nanoparticles, by adding surfactants to one or more of the solvents, and/or by performing ligand exchange or chemical modification to replace surface-bonded solvent or surfactant molecules with other functional groups to produce nanoparticles having the desired surface functionalization.

公开(公告)号：US11214661B2

公开(公告)日：2022-01-04

申请号：US15261163

申请日：2016-09-09

申请人： Massachusetts Institute of Technology

发明人： Samuel G. Rodriques ,  Daniel Oran ,  Ruixuan Gao ,  Shoh Asano ,  Mark A. Skylar-Scott ,  Fei Chen ,  Paul W. Tillberg ,  Adam H. Marblestone ,  Edward S. Boyden

IPC分类号： C08J7/12 ,  C08J7/02 ,  B29C35/08 ,  C08J3/075 ,  C08J3/28 ,  B33Y80/00 ,  B29K105/00

摘要： The present invention enables three-dimensional nanofabrication by isotropic shrinking of patterned hydrogels. A hydrogel is first expanded, the rate of expansion being controlled by the concentration of the crosslinker. The hydrogel is then infused with a reactive group and patterned in three dimensions using a photon beam through a limited-diffraction microscope. Functional particles or materials are then deposited on the pattern. The hydrogel is then shrunk and cleaved from the pattern.

公开(公告)号：US20240174818A1

公开(公告)日：2024-05-30

申请号：US18523580

申请日：2023-11-29

申请人： Massachusetts Institute of Technology

发明人： Ruixuan Gao ,  Linyi Gao ,  Chih-Chieh Yu ,  Edward Stuart Boyden

IPC分类号： C08J3/075 ,  C08F8/00 ,  C08F8/12 ,  C08F8/30 ,  C08F8/44 ,  C08F222/10 ,  C08G65/26 ,  C08G65/32 ,  C08G81/02 ,  C08J3/24 ,  C12Q1/68 ,  C12Q1/6834 ,  C12Q1/6841 ,  C12Q1/6869 ,  G01N1/30 ,  G01N33/545 ,  C08F220/04 ,  C08F220/30 ,  C08F220/34 ,  G01N1/36 ,  G01N15/0205

CPC分类号： C08J3/075 ,  C08F8/00 ,  C08F8/12 ,  C08F8/30 ,  C08F8/44 ,  C08F222/104 ,  C08G65/2624 ,  C08G65/32 ,  C08G81/025 ,  C08J3/246 ,  C12Q1/68 ,  C12Q1/6834 ,  C12Q1/6841 ,  C12Q1/6869 ,  G01N1/30 ,  G01N33/545 ,  C08F220/04 ,  C08F220/303 ,  C08F220/346 ,  C08F2438/01 ,  C08G2210/00 ,  C08J2300/206 ,  C08J2400/206 ,  C12Q2523/101 ,  G01N1/36 ,  G01N15/0205

摘要： The invention encompasses hydrogels, monomer precursors of the hydrogels, methods for the preparation thereof, and methods of use therefor. The linking of monomers can take place using non-radical, bioorthogonal reactions such as copper-free click-chemistry.

公开(公告)号：US11873374B2

公开(公告)日：2024-01-16

申请号：US16267849

申请日：2019-02-05

申请人： Massachusetts Institute of Technology

发明人： Ruixuan Gao ,  Linyi Gao ,  Chih-Chieh Yu ,  Edward Stuart Boyden

IPC分类号： C08J3/075 ,  C08G65/26 ,  C12Q1/6834 ,  C12Q1/6841 ,  G01N33/545 ,  C08G81/02 ,  C08F8/12 ,  G01N1/30 ,  C08J3/24 ,  C12Q1/68 ,  C08F8/30 ,  C08G65/32 ,  C12Q1/6869 ,  C08F8/44 ,  C08F8/00 ,  C08F222/10 ,  C08F220/04 ,  G01N15/02 ,  G01N1/36 ,  C08F220/30 ,  C08F220/34

CPC分类号： C08J3/075 ,  C08F8/00 ,  C08F8/12 ,  C08F8/30 ,  C08F8/44 ,  C08F222/104 ,  C08G65/2624 ,  C08G65/32 ,  C08G81/025 ,  C08J3/246 ,  C12Q1/68 ,  C12Q1/6834 ,  C12Q1/6841 ,  C12Q1/6869 ,  G01N1/30 ,  G01N33/545 ,  C08F220/04 ,  C08F220/303 ,  C08F220/346 ,  C08F2438/01 ,  C08G2210/00 ,  C08J2300/206 ,  C08J2400/206 ,  C12Q2523/101 ,  G01N1/36 ,  G01N15/0205 ,  C08F8/12 ,  C08F8/30 ,  C08F20/18 ,  C08F8/12 ,  C08F8/00 ,  C08F8/04 ,  C08F8/30 ,  C08F20/18 ,  C08F8/44 ,  C08F8/30 ,  C08F20/18 ,  C08F8/44 ,  C08F8/00 ,  C08F8/04 ,  C08F8/30 ,  C08F20/18 ,  C12Q1/6869 ,  C12Q2521/107 ,  C12Q2525/191 ,  C12Q2527/125 ,  C12Q2543/101

摘要： The invention encompasses hydrogels, monomer precursors of the hydrogels, methods for the preparation thereof, and methods of use therefor. The linking of monomers can take place using non-radical, bioorthogonal reactions such as copper-free click-chemistry.

公开(公告)号：US20170081489A1

公开(公告)日：2017-03-23

申请号：US15261163

申请日：2016-09-09

申请人： Massachusetts Institute of Technology

发明人： Samuel G. Rodriques ,  Daniel Oran ,  Ruixuan Gao ,  Shoh Asano ,  Mark A. Skylar-Scott ,  Fei Chen ,  Paul W. Tillberg ,  Adam H. Marblestone ,  Edward S. Boyden

IPC分类号： C08J7/12 ,  C08J7/02 ,  B29C35/08 ,  C08J3/075

CPC分类号： C08J7/123 ,  B29C35/0805 ,  B29C2035/0838 ,  B29K2105/0061 ,  B33Y80/00 ,  C08J3/075 ,  C08J3/28 ,  C08J7/02 ,  C08J7/12 ,  C08J2333/26

摘要： The present invention enables three-dimensional nanofabrication by isotropic shrinking of patterned hydrogels. A hydrogel is first expanded, the rate of expansion being controlled by the concentration of the crosslinker. The hydrogel is then infused with a reactive group and patterned in three dimensions using a photon beam through a limited-diffraction microscope. Functional particles or materials are then deposited on the pattern. The hydrogel is then shrunk and cleaved from the pattern.