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公开(公告)号：US10059990B2
公开(公告)日：2018-08-28
申请号：US15098968
申请日：2016-04-14
Inventor： Edward Stuart Boyden , Fei Chen , Shahar Alon , George Church , Paul Warren Tillberg , Adam Marblestone , Evan Daugharthy
IPC: C12Q1/68 , C12Q1/6874 , C12Q1/6806
CPC classification number: C12Q1/6874 , C12Q1/6806 , C12Q2521/537 , C12Q2523/308 , C12Q2547/101 , C12Q2565/514
Abstract: The invention provides in situ nucleic acid sequencing to be conducted in biological specimens that have been physically expanded. The invention leverages the techniques for expansion microscopy (ExM) to provide new methods for in situ sequencing of nucleic acids as well as new methods for fluorescent in situ sequencing (FISSEQ) in a new process referred to herein as “expansion sequencing” (ExSEQ).
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公开(公告)号：US20180216161A1
公开(公告)日：2018-08-02
申请号：US15876347
申请日：2018-01-22
Applicant: Massachusetts Institute of Technology
Inventor： Fei Chen , Asmamaw T. Wassie , Shahar Alon , Adam Henry Marblestone , Anubhav Sinha , Andrew Payne , Edward Stuart Boyden
IPC: C12Q1/6806 , C12Q1/6811 , C12Q1/682 , C12Q1/6855
CPC classification number: C12Q1/6806 , C12Q1/6811 , C12Q1/682 , C12Q1/6841 , C12Q1/6855 , C12Q2600/158 , C12Q2600/16 , C12Q2600/166 , C12Q2533/107 , C12Q2543/101
Abstract: The present invention relates to a method for amplifying at least one target RNA in a fixed and, optionally, expanded biological sample. In an embodiment of the invention, the method comprises incubating the fixed biological sample with a pair of polynucleotides complementary to non-overlapping and proximal sequences of a target RNA, wherein the polynucleotide pair hybridizes to the target RNA; ligating the polynucleotide pair using a ligase; and amplifying the ligation product. The invention further provides methods for detecting and optionally quantifying and/or sequencing the amplification product. As the method comprises hybridizing polynucleotide pairs to a target RNA in a fixed biological sample, the target RNA can be hybridized in situ.
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公开(公告)号：US20170081489A1
公开(公告)日：2017-03-23
申请号：US15261163
申请日：2016-09-09
Applicant: Massachusetts Institute of Technology
Inventor： Samuel G. Rodriques , Daniel Oran , Ruixuan Gao , Shoh Asano , Mark A. Skylar-Scott , Fei Chen , Paul W. Tillberg , Adam H. Marblestone , Edward S. Boyden
CPC classification number: C08J7/123 , B29C35/0805 , B29C2035/0838 , B29K2105/0061 , B33Y80/00 , C08J3/075 , C08J3/28 , C08J7/02 , C08J7/12 , C08J2333/26
Abstract: The present invention enables three-dimensional nanofabrication by isotropic shrinking of patterned hydrogels. A hydrogel is first expanded, the rate of expansion being controlled by the concentration of the crosslinker. The hydrogel is then infused with a reactive group and patterned in three dimensions using a photon beam through a limited-diffraction microscope. Functional particles or materials are then deposited on the pattern. The hydrogel is then shrunk and cleaved from the pattern.
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公开(公告)号：US20160254512A1
公开(公告)日：2016-09-01
申请号：US15055491
申请日：2016-02-26
Inventor： Huayi Yin , Fei Chen , Brice Hoani Valentin Chung , Takanari Ouchi , Donald Robert Sadoway
CPC classification number: H01M10/399 , H01M2/1646 , H01M2/1673 , H01M4/38 , H01M4/382 , H01M4/387 , H01M4/405 , H01M2300/0057
Abstract: An electrochemical cell includes a negative electrode having a first liquid phase having a first active metal, a positive electrode having a second liquid phase having a second active metal, and a liquid electrolyte having a salt of the first active metal and a salt of the second active metal. The electrochemical cell also includes a bipolar faradaic membrane, disposed between the negative electrode and the positive electrode, having a first surface facing the negative electrode and a second surface facing the positive electrode. The bipolar faradaic membrane is configured to allow cations of the first active metal to pass through and to impede cations of the second active metal from transferring from the positive electrode to the negative electrode and is at least partially formed from a material having an electronic conductivity sufficient to drive faradaic reactions at the second surface with the cations of the positive electrode.
Abstract translation: 电化学电池包括具有第一液相的第一活性金属的负极，具有第二活性金属的第二液相的正极和具有第一活性金属的盐和第二活性金属的盐的液体电解质 活性金属。 电化学电池还包括设置在负电极和正电极之间的双极法拉第膜，具有面向负电极的第一表面和面对正电极的第二表面。 双极法拉第膜被配置为允许第一活性金属的阳离子通过并阻止第二活性金属的阳离子从正极转移到负极，并且至少部分地由具有足够的电子传导性的材料形成 以正电极的阳离子在第二表面驱动法拉第反应。
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公开(公告)号：US12195734B2
公开(公告)日：2025-01-14
申请号：US17806879
申请日：2022-06-14
Applicant: Massachusetts Institute of Technology , The Broad Institute, Inc. , President and Fellows of Harvard College
Inventor： Kaiyi Jiang , Rohan Neil Krajeski , Omar Osama Abudayyeh , Jonathan S. Gootenberg , Yifan Zhang , Fei Chen , Xi Chen , Jeremy G. Koob
IPC: C12N15/113 , C12N9/50 , C12N9/78 , C12N15/10 , C12Q1/6897
Abstract: RNA editing tools for use in systems designed to measure RNA in vivo and manipulate specific cell types are disclosed herein. An RNA sensor system comprising a) a single-stranded RNA (ssRNA) sensor comprising a stop codon and a payload; optionally wherein the ssRNA sensor further comprises a normalizing gene; and b) an adenosine deaminase acting on RNA (ADAR) deaminase; wherein the sensor is capable of binding to a ssRNA target to form a double-stranded RNA (dsRNA) duplex that becomes a substrate for the ADAR deaminase; wherein the substrate comprises a mispairing within the stop codon; and wherein the mispairing is editable by the ADAR deaminase, which editing can effectively remove the stop codon so as to enable translation and expression of the payload. A method of quantifying ribonucleic acid (RNA) levels using the RNA sensor system is also disclosed.
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Patent Agency Ranking
公开(公告)号：US11414701B2
公开(公告)日：2022-08-16
申请号：US16422837
申请日：2019-05-24
Applicant: THE BROAD INSTITUTE, INC. , PRESIDENT AND FELLOWS OF HARVARD COLLEGE , MASSACHUSETTS INSTITUTE OF TECHNOLOGY
Inventor： Fei Chen , Jesse Engreitz , Jamie Marshall , Vidya Subramanian , Sam Rodriques
IPC: C12Q1/6874 , C12Q1/6876 , C12N15/10
Abstract: Provided herein are methods for generating single-cell molecular analysis comprising a) delivering one or more proximity dependent probes to a cell population, wherein each proximity dependent probe comprises a target binding region configured to bind a target RNA and a primer binding site region; b) linking bound proximity dependent probes; c) isolating single cells from the cell population in separate individual discrete volumes, the individual discrete volumes further comprising a primer pair and amplification reagents, wherein the primer pair binds to the primer binding sites of the ligation dependent probes, and wherein at least one primer comprises a barcode sequence that uniquely identifies the individual discrete volume; d) amplifying the ligated probes using the primer pair, wherein the barcode is incorporated into each resulting amplicon; and e) quantifying target RNAs in each individual cell based at least in part on sequencing the resulting amplicons.
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公开(公告)号：US11214661B2
公开(公告)日：2022-01-04
申请号：US15261163
申请日：2016-09-09
Applicant: Massachusetts Institute of Technology
Inventor： Samuel G. Rodriques , Daniel Oran , Ruixuan Gao , Shoh Asano , Mark A. Skylar-Scott , Fei Chen , Paul W. Tillberg , Adam H. Marblestone , Edward S. Boyden
Abstract: The present invention enables three-dimensional nanofabrication by isotropic shrinking of patterned hydrogels. A hydrogel is first expanded, the rate of expansion being controlled by the concentration of the crosslinker. The hydrogel is then infused with a reactive group and patterned in three dimensions using a photon beam through a limited-diffraction microscope. Functional particles or materials are then deposited on the pattern. The hydrogel is then shrunk and cleaved from the pattern.
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公开(公告)号：US20210123040A1
公开(公告)日：2021-04-29
申请号：US17051793
申请日：2019-05-01
Applicant: THE GENERAL HOSPITAL CORPORATION , THE BROAD INSTITUTE, INC. , MASSACHUSETTS INSTITUTE OF TECHNOLOGY , PRESIDENT AND FELLOWS OF HARVARD COLLEGE
Inventor： Evan Macosko , Fei Chen , Sam Rodriques , Robert Stickels , Carly Martin
IPC: C12N15/10 , C12Q1/6841
Abstract: The present disclosure relates to compositions and methods for assessing relative macromolecule abundance (e.g., RNA expression levels) in a spatially-defined manner across a tissue sample, specifically providing deep transcriptomic coverage at high-resolution across multiple locations assessed across the tissue sample.
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公开(公告)号：US10563257B2
公开(公告)日：2020-02-18
申请号：US16110150
申请日：2018-08-23
Inventor： Edward Stuart Boyden , Fei Chen , Shahar Alon , George Church , Paul Warren Tillberg , Adam Henry Marblestone , Evan R. Daugharthy
IPC: C12Q1/6874 , C12Q1/6806
Abstract: The invention provides in situ nucleic acid sequencing to be conducted in biological specimens that have been physically expanded. The invention leverages the techniques for expansion microscopy (ExM) to provide new methods for in situ sequencing of nucleic acids as well as new methods for fluorescent in situ sequencing (FISSEQ) in a new process referred to herein as “expansion sequencing” (ExSEQ).
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公开(公告)号：US10396404B2
公开(公告)日：2019-08-27
申请号：US15055491
申请日：2016-02-26
Inventor： Huayi Yin , Fei Chen , Brice Hoani Valentin Chung , Takanari Ouchi , Donald Robert Sadoway
Abstract: An electrochemical cell includes a negative electrode having a first liquid phase having a first active metal, a positive electrode having a second liquid phase having a second active metal, and a liquid electrolyte having a salt of the first active metal and a salt of the second active metal. The electrochemical cell also includes a bipolar faradaic membrane, disposed between the negative electrode and the positive electrode, having a first surface facing the negative electrode and a second surface facing the positive electrode. The bipolar faradaic membrane is configured to allow cations of the first active metal to pass through and to impede cations of the second active metal from transferring from the positive electrode to the negative electrode and is at least partially formed from a material having an electronic conductivity sufficient to drive faradaic reactions at the second surface with the cations of the positive electrode.
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