Polyol oxidases
    1.
    发明授权
    Polyol oxidases 有权
    多元氧化酶

    公开(公告)号:US07919295B2

    公开(公告)日:2011-04-05

    申请号:US11875788

    申请日:2007-10-19

    摘要: The present invention provides compositions and methods for producing a polyol oxidase in microorganisms, and the use of polyol oxidases in cleaning compositions. The invention includes cleaning compositions that contain combinations of two or more POx oxidases, and cleaning compositions that contain combinations of two or more POx oxidases and a perhydrolase. In particular, the invention provides methods for expressing polyol oxidases in bacterial hosts for use in detergent applications for cleaning, bleaching and disinfecting.

    摘要翻译: 本发明提供了用于在微生物中生产多元醇氧化酶的组合物和方法,以及在清洁组合物中使用多元醇氧化酶。 本发明包括含有两种或更多种POx氧化酶的组合的清洁组合物和含有两种或更多种POx氧化酶和过水解酶的组合的清洁组合物。 特别地,本发明提供了用于在洗涤剂应用中用于清洁,漂白和消毒的细菌宿主中表达多元氧化酶的方法。

    POLYOL OXIDASES
    4.
    发明申请
    POLYOL OXIDASES 有权
    多环氧化物

    公开(公告)号:US20080293611A1

    公开(公告)日:2008-11-27

    申请号:US11875788

    申请日:2007-10-19

    摘要: The present invention provides compositions and methods for producing a polyol oxidase in micoroorganisms, and the use of polyol oxidases in cleaning compositions. The invention includes cleaning compositions that contain combinations of two or more POx oxidases, and cleaning compositions that contain combinations of two or more POx oxidases and a perhydrolase. In particular, the invention provides methods for expressing polyol oxidases in bacterial hosts for use in detergent applications for cleaning, bleaching and disinfecting.

    摘要翻译: 本发明提供用于在微生物中生产多元醇氧化酶的组合物和方法,以及在清洁组合物中使用多元醇氧化酶。 本发明包括含有两种或更多种POx氧化酶的组合的清洁组合物和含有两种或更多种POx氧化酶和过水解酶的组合的清洁组合物。 特别地,本发明提供了用于在洗涤剂应用中用于清洁,漂白和消毒的细菌宿主中表达多元氧化酶的方法。

    KEX2 cleavage regions of recombinant fusion proteins
    5.
    发明授权
    KEX2 cleavage regions of recombinant fusion proteins 有权
    重组融合蛋白的KEX2切割区

    公开(公告)号:US08518667B2

    公开(公告)日:2013-08-27

    申请号:US13472296

    申请日:2012-05-15

    摘要: The invention relates to a fusion DNA construct comprising a KEX2 region comprising a KEX2 site and a KEX2 site pre-sequence immediately 5′ to the KEX2 site, a fusion polypeptide, vectors and cells comprising the fusion DNA construct, methods for producing desired proteins from filamentous fungal cells and methods for enhancing the secretion and/or cleavage of a desired protein from a cell.

    摘要翻译: 本发明涉及包含KEX2区域的融合DNA构建体,所述KEX2区域包含KEX2位点和紧邻KEX2位点5'的KEX2位点前序列,融合多肽,载体和包含融合DNA构建体的细胞,用于产生所需蛋白质的方法 丝状真菌细胞和用于增强从细胞中分泌和/或切割所需蛋白质的方法。

    KEX2 cleavage regions of recombinant fusion proteins
    6.
    发明授权
    KEX2 cleavage regions of recombinant fusion proteins 有权
    重组融合蛋白的KEX2切割区

    公开(公告)号:US08198046B2

    公开(公告)日:2012-06-12

    申请号:US12373121

    申请日:2007-06-21

    摘要: The invention relates to a fusion DNA construct comprising a KEX2 region comprising a KEX2 site and a KEX2 site pre-sequence immediately 5′ to the KEX2 site, a fusion polypeptide, vectors and cells comprising the fusion DNA construct, methods for producing desired proteins from filamentous fungal cells and methods for enhancing the secretion and/or cleavage of a desired protein from a cell.

    摘要翻译: 本发明涉及包含KEX2区域的融合DNA构建体,所述KEX2区域包含KEX2位点和紧邻KEX2位点5'的KEX2位点前序列,融合多肽,载体和包含融合DNA构建体的细胞,用于产生所需蛋白质的方法 丝状真菌细胞和用于增强从细胞中分泌和/或切割所需蛋白质的方法。

    Production of functional antibodies in filamentous fungi
    8.
    发明授权
    Production of functional antibodies in filamentous fungi 有权
    生产丝状真菌中的功能性抗体

    公开(公告)号:US07977067B2

    公开(公告)日:2011-07-12

    申请号:US11007886

    申请日:2004-12-09

    IPC分类号: C07K16/46

    摘要: Described herein are methods for the production of monoclonal antibodies in filamentous fungi host cells. The monoclonal antibodies are expressed as full-length fusion proteins that retain functional antigen binding and antibody-dependent cellular cytotoxicity capabilities. Improvements in the cleavage of the glucoamylase-light chain fusion protein to yield a mature antibody are also provided. The antibodies produced in filamentous fungi show equivalent pharmacokinetic disposition to antibodies produced in mammalian cells.

    摘要翻译: 本文描述了在丝状真菌宿主细胞中生产单克隆抗体的方法。 单克隆抗体表达为全长融合蛋白,其保留功能性抗原结合和抗体依赖性细胞毒性能力。 还提供了葡糖淀粉酶 - 轻链融合蛋白切割产生成熟抗体的改进。 在丝状真菌中产生的抗体对哺乳动物细胞中产生的抗体显示出等效的药代动力学位置。

    FILAMENTOUS FUNGI WITH INACTIVATED PROTEASE GENES FOR ALTERED PROTEIN PRODUCTION
    9.
    发明申请
    FILAMENTOUS FUNGI WITH INACTIVATED PROTEASE GENES FOR ALTERED PROTEIN PRODUCTION 审中-公开
    FILAMENTOUS FUNGI WITH INVTIVED PROTEASE GENES FOR ALTERED PROTEIN PRODUCTION

    公开(公告)号:US20090253173A1

    公开(公告)日:2009-10-08

    申请号:US12412297

    申请日:2009-03-26

    申请人: Huaming Wang

    发明人: Huaming Wang

    摘要: The invention relates to a filamentous fungal cell (e.g., Aspergillus sp.) comprising at least one inactivated protease gene chosen from apsB, a homolog of apsB, cpsA, a homolog cpsA, and combinations thereof. Nucleic acids and methods for making the inactivated mutant filamentous fungal cells are provided as well as methods for using the cells for the altered production of endogenous or heterologous proteins of interest.

    摘要翻译: 本发明涉及包含至少一种选自apsB的灭活蛋白酶基因,apsB同源物,cpsA,同系物cpsA及其组合的丝状真菌细胞(例如曲霉属)。 提供用于制备灭活的突变丝状真菌细胞的核酸和方法以及使用细胞改变产生感兴趣的内源或异源蛋白质的方法。