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公开(公告)号：US20230183760A1
公开(公告)日：2023-06-15
申请号：US18172594
申请日：2023-02-22
申请人： MANUS BIO INC.
发明人： Ryan N. PHILIPPE , Ajikumar PARAYIL KUMARAN , Christine Nicole S. SANTOS , Jason DONALD , Stephen SARRIA
CPC分类号： C12P7/26 , C12N9/0006 , C12N9/0071 , C12N9/1085 , C12N9/88 , C12N15/52 , C12Y205/0101 , C12Y402/03087
摘要： The present disclosure provides methods and compositions for producing rotundone. In various aspects, the present disclosure provides enzymes, polynucleotides encoding said enzymes, and recombinant microbial host cells (or microbial host strains) for the production of rotundone. In some embodiments, the present disclosure provides microbial host cells for producing rotundone at high purity and/or yield, from either enzymatic transformation of α-guaiene, or from sugar or other carbon source. The present disclosure further provides methods of making products containing rotundone, including flavor or fragrance products, among others.
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公开(公告)号：US20230042171A1
公开(公告)日：2023-02-09
申请号：US17785488
申请日：2020-12-16
申请人： Manus Bio Inc.
发明人： Michelle N. GOETTGE , Ryan N. PHILIPPE , Ajikumar Parayil KUMARAN , Christine Nicole S. SANTOS , Jason Eric DONALD , Christopher FREI , Aaron LOVE , Kaitlin CHAMBERS , Joyce SAMSON , Christopher TOOMEY
摘要： The present invention provides host cells and methods for making mogrol glycosides, including Mogroside V (Mog.V), Mogroside VI (Mog.VI), Iso-Mogroside V (Isomog.V), siamenoside, and glycosylation products that are minor products in Siraitia grosvenorii. The invention provides engineered enzymes and engineered host cells for producing mogrol glycosylation products, such as Mog.V, Mog.VI, and Isomog.V, at high purity and/or yield. The present technology further provides methods of making products containing mogrol glycosides, such as Mog.V, Mog.VI, and Isomog.V, including food products, beverages, oral care products, sweeteners, and flavoring products.
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公开(公告)号：US20220162658A1
公开(公告)日：2022-05-26
申请号：US17546758
申请日：2021-12-09
申请人： Manus Bio Inc.
发明人： Ajikumar Parayil KUMARAN , Christine Nicole S. SANTOS , Jason Eric DONALD , Mary Elizabeth FOWLER , Ryan N. PHILIPPE , Christopher Scott FREI , Aaron LOVE
IPC分类号： C12P19/18
摘要： In various aspects and embodiments, the invention provides microbial cells and methods for producing advanced glycosylation products from lower glycosylated intermediates. The microbial cell expresses one or more UDP-dependent glycosyl transferase enzymes in the cytoplasm, for glycosylation of the intermediates. When incubating the microbial strain with a plant extract or fraction thereof comprising the intermediates, these glycosylated intermediates are available for further glycosylation by the cell, and the advanced glycosylation products can be recovered from the media and/or microbial cells.
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公开(公告)号：US20200087692A1
公开(公告)日：2020-03-19
申请号：US16512874
申请日：2019-07-16
申请人： Manus Bio, Inc.
发明人： Ajikumar Parayil KUMARAN , Christine Nicole S. SANTOS , Jason Eric DONALD , Mary Elizabeth FOWLER , Ryan N. PHILIPPE , Christopher Scott FREI , Aaron LOVE
IPC分类号： C12P19/18
摘要： In various aspects and embodiments, the invention provides microbial cells and methods for producing advanced glycosylation products from lower glycosylated intermediates. The microbial cell expresses one or more UDP-dependent glycosyl transferase enzymes in the cytoplasm, for glycosylation of the intermediates. When incubating the microbial strain with a plant extract or fraction thereof comprising the intermediates, these glycosylated intermediates are available for further glycosylation by the cell, and the advanced glycosylation products can be recovered from the media and/or microbial cells.
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