公开(公告)号：US08858940B2

公开(公告)日：2014-10-14

申请号：US13133685

申请日：2009-12-10

申请人： Maria Gonzalez Pajuelo ,  Michael John Scott Saunders ,  Johannes Joseph Wilhelmus De Haard ,  Peter Vanlandschoot

发明人： Maria Gonzalez Pajuelo ,  Michael John Scott Saunders ,  Johannes Joseph Wilhelmus De Haard ,  Peter Vanlandschoot

IPC分类号： C12P21/08 ,  C07K16/28 ,  C07K16/22

CPC分类号： C07K16/22 ,  C07K16/2863 ,  C07K16/464 ,  C07K2317/22 ,  C07K2317/31 ,  C07K2317/565 ,  C07K2317/567 ,  C07K2317/569 ,  C07K2317/76

摘要： The present invention relates to amino acid sequences that are directed against proteins from the group of the Angiopoietin/Tie family such as Tie1, Tie2, Ang1, Ang2, Ang3, Ang4, Angptl1, Angptl12, Angptl13, Angptl14, Angptl15, Angptl16, as well as to compounds or constructs, and in particular proteins and polypeptides, that comprise or essentially consist of one or more of such amino acid sequences.

摘要翻译： 本发明涉及针对来自促血管生成素/ Tie家族的蛋白质的氨基酸序列，例如Tie1，Tie2，Ang1，Ang2，Ang3，Ang4，Angpt1，Angptl12，Angpt13，Angpt14，​​Angptl15，Angptl16，以及 关于包含或基本上由一个或多个这样的氨基酸序列组成的化合物或构建体，特别是蛋白质和多肽。

公开(公告)号：US20120076796A1

公开(公告)日：2012-03-29

申请号：US13133685

申请日：2009-12-10

申请人： Maria Gonzalez Pajuelo ,  Michael John Scott Saunders ,  Johannes Joseph Wilhelmus De Haard ,  Peter Vanlandschoot

发明人： Maria Gonzalez Pajuelo ,  Michael John Scott Saunders ,  Johannes Joseph Wilhelmus De Haard ,  Peter Vanlandschoot

IPC分类号： A61K39/395 ,  A61P9/10 ,  C12P21/00 ,  A61P35/00 ,  C07K16/22 ,  C07K16/40

CPC分类号： C07K16/22 ,  C07K16/2863 ,  C07K16/464 ,  C07K2317/22 ,  C07K2317/31 ,  C07K2317/565 ,  C07K2317/567 ,  C07K2317/569 ,  C07K2317/76

摘要： The present invention relates to amino acid sequences that are directed against proteins from the group of the Angiopoietin/Tie family such as Tie1, Tie2, Ang1, Ang2, Ang3, Ang4, Angptl1, Angptl12, Angptl13, Angptl14, Angptl15, Angptl16, as well as to compounds or constructs, and in particular proteins and polypeptides, that comprise or essentially consist of one or more of such amino acid sequences.

摘要翻译： 本发明涉及针对来自促血管生成素/ Tie家族的蛋白质的氨基酸序列，例如Tie1，Tie2，Ang1，Ang2，Ang3，Ang4，Angpt1，Angptl12，Angpt13，Angpt14，​​Angptl15，Angptl16，以及 关于包含或基本上由一个或多个这样的氨基酸序列组成的化合物或构建体，特别是蛋白质和多肽。

公开(公告)号：US20110282033A1

公开(公告)日：2011-11-17

申请号：US12600023

申请日：2008-05-23

申请人： Maria Gonzalez Pajuelo ,  Michael John Scott Saunders ,  Johannes Joseph Wilhelmus De Haard ,  Edward Dolk ,  Soren Steffensen

发明人： Maria Gonzalez Pajuelo ,  Michael John Scott Saunders ,  Johannes Joseph Wilhelmus De Haard ,  Edward Dolk ,  Soren Steffensen

IPC分类号： C07K16/28 ,  C07K16/40

CPC分类号： C07K16/2863 ,  C07K2317/22 ,  C07K2317/569 ,  C07K2317/76 ,  C07K2319/32

摘要： The present invention relates to amino acid sequences that are directed against receptors for growth factors, compounds comprising such sequences, as well as nucleic acid sequences encoding the same. In one embodiment the invention relates to amino acid sequences that bind to a receptor tyrosine kinase. The receptor tyrosine kinase and/or growth factor receptor may be of human origin. In one embodiment the amino acid sequences are Nanobodies™.

摘要翻译： 本发明涉及针对生长因子受体的氨基酸序列，包含该序列的化合物以及编码该氨基酸序列的核酸序列。 在一个实施方案中，本发明涉及结合受体酪氨酸激酶的氨基酸序列。 受体酪氨酸激酶和/或生长因子受体可能是人类来源的。 在一个实施方案中，氨基酸序列是纳米抗体TM。

公开(公告)号：US20110262438A1

公开(公告)日：2011-10-27

申请号：US13073044

申请日：2011-03-28

申请人： Francis Descamps ,  Maria Gonzalez Pajuelo ,  Pascal Gerard Merchiers ,  Catelijne Stortelers ,  Peter Vanlandschoot ,  Philippe Van Rompaey ,  Martine Smit ,  Regorius Leurs ,  David Andre Baptiste Maussang Detaille

发明人： Francis Descamps ,  Maria Gonzalez Pajuelo ,  Pascal Gerard Merchiers ,  Catelijne Stortelers ,  Peter Vanlandschoot ,  Philippe Van Rompaey ,  Martine Smit ,  Regorius Leurs ,  David Andre Baptiste Maussang Detaille

IPC分类号： A61K39/395 ,  C12N15/13 ,  C12P21/08 ,  A61P35/00 ,  A61P17/06 ,  A61P25/00 ,  A61P29/00 ,  A61P11/06 ,  A61P9/10 ,  A61P37/06 ,  C07K16/28 ,  A61P25/28

CPC分类号： C07K16/2866 ,  A61K39/39558 ,  A61K2039/505 ,  C07K2317/22 ,  C07K2317/33 ,  C07K2317/565 ,  C07K2317/569 ,  C07K2317/76 ,  C07K2317/92

摘要： The present invention relates to particular polypeptides, nucleic acids encoding such polypeptides; to methods for preparing such polypeptides; to host cells expressing or capable of expressing such polypeptides; to compositions and in particular to pharmaceutical compositions that comprise such polypeptides, for prophylactic, therapeutic or diagnostic purposes.

摘要翻译： 本发明涉及特定的多肽，编码这种多肽的核酸; 制备这种多肽的方法; 以宿主表达或能够表达这种多肽的细胞; 组合物，特别是涉及用于预防，治疗或诊断目的的包含这种多肽的药物组合物。

公开(公告)号：US20150158948A9

公开(公告)日：2015-06-11

申请号：US14008011

申请日：2012-03-28

申请人： Francis Descamps ,  David André Baptiste Maussang-Detaille ,  Maarten Van Roy ,  Regorius Leurs ,  Maria Gonzalez Pajuelo ,  Pascal Gerard Merchiers ,  Martine Smit ,  Catelijne Stortelers ,  Philippe Van Rompaey ,  Peter Vanlandschoot

发明人： Francis Descamps ,  David André Baptiste Maussang-Detaille ,  Maarten Van Roy ,  Regorius Leurs ,  Maria Gonzalez Pajuelo ,  Pascal Gerard Merchiers ,  Martine Smit ,  Catelijne Stortelers ,  Philippe Van Rompaey ,  Peter Vanlandschoot

IPC分类号： C07K16/28

CPC分类号： C07K16/2866 ,  A61K2039/507 ,  C07K2317/22 ,  C07K2317/31 ,  C07K2317/33 ,  C07K2317/569 ,  C07K2317/76 ,  C07K2319/31

摘要： The present invention relates to particular polypeptides, nucleic acids encoding such polypeptides; to methods for preparing such polypeptides; to host cells expressing or capable of expressing such polypeptides; to compositions and in particular to pharmaceutical compositions that comprise such polypeptides, for prophylactic, therapeutic or diagnostic purposes. In particular, the present invention provides immunoglobulin single variable domains inhibiting CXCR7 mediated tumour growth.

摘要翻译： 本发明涉及特定的多肽，编码这种多肽的核酸; 制备这种多肽的方法; 以宿主表达或能够表达这种多肽的细胞; 组合物，特别是涉及用于预防，治疗或诊断目的的包含这种多肽的药物组合物。 特别地，本发明提供了抑制CXCR7介导的肿瘤生长的免疫球蛋白单可变结构域。

公开(公告)号：US20140227270A1

公开(公告)日：2014-08-14

申请号：US14008011

申请日：2012-03-28

申请人： Francis Descamps ,  David André Baptiste Maussang-Detaille ,  Maarten Van Roy ,  Regorius Leurs ,  Maria Gonzalez Pajuelo ,  Pascal Gerard Merchiers ,  Martine Smit ,  Catelijne Stortelers ,  Philippe Van Rompaey ,  Peter Vanlandschoot

发明人： Francis Descamps ,  David André Baptiste Maussang-Detaille ,  Maarten Van Roy ,  Regorius Leurs ,  Maria Gonzalez Pajuelo ,  Pascal Gerard Merchiers ,  Martine Smit ,  Catelijne Stortelers ,  Philippe Van Rompaey ,  Peter Vanlandschoot

IPC分类号： C07K16/28

CPC分类号： C07K16/2866 ,  A61K2039/507 ,  C07K2317/22 ,  C07K2317/31 ,  C07K2317/33 ,  C07K2317/569 ,  C07K2317/76 ,  C07K2319/31

摘要： The present invention relates to particular polypeptides, nucleic acids encoding such polypeptides; to methods for preparing such polypeptides; to host cells expressing or capable of expressing such polypeptides; to compositions and in particular to pharmaceutical compositions that comprise such polypeptides, for prophylactic, therapeutic or diagnostic purposes. In particular, the present invention provides immunoglobulin single variable domains inhibiting CXCR7 mediated tumour growth.

摘要翻译： 本发明涉及特定的多肽，编码这种多肽的核酸; 制备这种多肽的方法; 以宿主表达或能够表达这种多肽的细胞; 组合物，特别是涉及用于预防，治疗或诊断目的的包含这种多肽的药物组合物。 特别地，本发明提供了抑制CXCR7介导的肿瘤生长的免疫球蛋白单可变结构域。

公开(公告)号：US20110117113A1

公开(公告)日：2011-05-19

申请号：US12897051

申请日：2010-10-04

申请人： Gerald Beste ,  Stephanie Staelens ,  Peter Vanlandschoot ,  Maria Gonzalez Pajuelo ,  Hilde Adi Pierrette Revets ,  Peter Schotte ,  Hilde Stals ,  Ann Brige ,  Maarten Dewilde ,  Catelijne Stortelers

发明人： Gerald Beste ,  Stephanie Staelens ,  Peter Vanlandschoot ,  Maria Gonzalez Pajuelo ,  Hilde Adi Pierrette Revets ,  Peter Schotte ,  Hilde Stals ,  Ann Brige ,  Maarten Dewilde ,  Catelijne Stortelers

IPC分类号： A61K39/395 ,  C07K16/28 ,  C07K16/46 ,  C07K19/00 ,  A61P35/00 ,  A61P35/02 ,  A61P35/04 ,  A61P29/00 ,  A61P31/00 ,  A61P17/06 ,  A61P11/06 ,  A61P11/00 ,  A61P37/06

CPC分类号： C07K16/2866 ,  C07K16/00 ,  C07K2317/22 ,  C07K2317/31 ,  C07K2317/565 ,  C07K2317/567 ,  C07K2317/569 ,  C07K2317/76 ,  C07K2317/92

摘要： The invention relates to immunoglobulin single variable domains directed against specific human CXCR4 epitopes (herein also referred to interchangeably as “compounds of the invention”, “amino acid sequences of the invention”, or “building blocks of the invention”) and polypeptides comprising them (herein also referred to as “polypeptides of the invention”, “compounds of the invention”, or “constructs of the invention”).Furthermore, the present invention relates to nucleic acids encoding the compounds of the invention (also referred to herein as “nucleic acids of the invention” or “nucleotide sequences of the invention”); to methods for preparing the compounds of the invention; to host cells expressing or capable of expressing the compounds of the invention; to compositions, and in particular to pharmaceutical compositions, that comprise the compounds of the invention; and to uses of the compounds of the invention and the aforementioned nucleic acids, host cells and/or compositions, in particular for prophylactic, therapeutic or diagnostic purposes, such as the prophylactic, therapeutic or diagnostic purposes mentioned herein.The invention also relates to methods for generating immunoglobulin single variable domains against a target such as a cell-associated protein and constructs comprising said immunoglobulin single variable domains. The invention also provides immunoglobulin single variable domains obtainable by the methods of the invention. Specifically, the present invention relates to the generation of immunoglobulin single variable domains and constructs thereof by use of epitope walking with multimer libraries. More specifically, the present invention relates to the generation of immunoglobulin single variable domains derived from camelids directed against a particular epitope of a target, in particular against a target with multiple transmembrane spanning domains, including GPCRs and ion channels, by epitope walking with multimer libraries.

公开(公告)号：US08937164B2

公开(公告)日：2015-01-20

申请号：US13073044

申请日：2011-03-28

申请人： Francis Descamps ,  Maria Gonzalez Pajuelo ,  Pascal Gerard Merchiers ,  Catelijne Stortelers ,  Peter Vanlandschoot ,  Philippe Van Rompaey ,  Martine Smit ,  Regorius Leurs ,  David Andre Baptiste Maussang Detaille

发明人： Francis Descamps ,  Maria Gonzalez Pajuelo ,  Pascal Gerard Merchiers ,  Catelijne Stortelers ,  Peter Vanlandschoot ,  Philippe Van Rompaey ,  Martine Smit ,  Regorius Leurs ,  David Andre Baptiste Maussang Detaille

IPC分类号： C07K16/28

CPC分类号： C07K16/2866 ,  A61K39/39558 ,  A61K2039/505 ,  C07K2317/22 ,  C07K2317/33 ,  C07K2317/565 ,  C07K2317/569 ,  C07K2317/76 ,  C07K2317/92

摘要： The present invention relates to particular polypeptides, nucleic acids encoding such polypeptides; to methods for preparing such polypeptides; to host cells expressing or capable of expressing such polypeptides; to compositions and in particular to pharmaceutical compositions that comprise such polypeptides, for prophylactic, therapeutic or diagnostic purposes.

摘要翻译： 本发明涉及特定的多肽，编码这种多肽的核酸; 制备这种多肽的方法; 以宿主表达或能够表达这种多肽的细胞; 组合物，特别是涉及用于预防，治疗或诊断目的的包含这种多肽的药物组合物。

公开(公告)号：US20100173799A1

公开(公告)日：2010-07-08

申请号：US12643286

申请日：2009-12-21

申请人： Toon Laeremans ,  Catelijne Stortelers ,  Friedrich Nolte ,  Maria Gonzalez Pajuelo ,  Joana Assunção ,  Philippe Van Rompaey

发明人： Toon Laeremans ,  Catelijne Stortelers ,  Friedrich Nolte ,  Maria Gonzalez Pajuelo ,  Joana Assunção ,  Philippe Van Rompaey

IPC分类号： C40B30/04

CPC分类号： C07K16/28 ,  A61K2039/505 ,  A61K2039/53 ,  C07K16/00 ,  C07K16/2866 ,  C07K2317/22 ,  C07K2317/34 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/567 ,  C07K2317/569 ,  C07K2317/75 ,  C07K2317/92

摘要： The present invention relates to a method for generating immunoglobulin sequences against cell-associated antigens, more particularly, antigens that are membrane-anchored. The invention also provides immunoglobulin sequences obtainable by the method of the invention. Specifically, the present invention relates to the generation of immunoglobulin sequences by use of DNA vaccination. More specifically, the present invention relates to generation of immunoglobulin sequences in camelids, preferably directed against cell-associated antigens, in particular antigens with multiple transmembrane spanning domains, including GPCRs and ion channels, by DNA vaccination. Furthermore, the present invention relates to said immunoglobulin sequences against cell-associated antigens, more particularly, antigens that are membrane-anchored, such as e.g. GPCRs and ion channels, more preferably ion channels.

摘要翻译： 本发明涉及产生针对细胞相关抗原的免疫球蛋白序列的方法，更具体地说，涉及膜锚定的抗原。 本发明还提供可通过本发明的方法获得的免疫球蛋白序列。 具体地说，本发明涉及使用DNA疫苗接种产生免疫球蛋白序列。 更具体地，本发明涉及通过DNA疫苗接种在骆驼中产生免疫球蛋白序列，优选针对细胞相关抗原，特别是具有多个跨膜跨越结构域的抗原，包括GPCR和离子通道。 此外，本发明涉及针对细胞相关抗原的所述免疫球蛋白序列，更具体地，涉及膜锚定的抗原，例如抗体。 GPCR和离子通道，更优选离子通道。

公开(公告)号：US09260508B2

公开(公告)日：2016-02-16

申请号：US12643286

申请日：2009-12-21

申请人： Toon Laeremans ,  Catelijne Stortelers ,  Friedrich Nolte ,  Maria Gonzalez Pajuelo ,  Joana Assunção ,  Philippe Van Rompaey

发明人： Toon Laeremans ,  Catelijne Stortelers ,  Friedrich Nolte ,  Maria Gonzalez Pajuelo ,  Joana Assunção ,  Philippe Van Rompaey

IPC分类号： C40B30/04 ,  A61K39/00 ,  C07K16/00 ,  C07K16/28

CPC分类号： C07K16/28 ,  A61K2039/505 ,  A61K2039/53 ,  C07K16/00 ,  C07K16/2866 ,  C07K2317/22 ,  C07K2317/34 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/567 ,  C07K2317/569 ,  C07K2317/75 ,  C07K2317/92

摘要： The present invention relates to a method for generating immunoglobulin sequences against cell-associated antigens, more particularly, antigens that are membrane-anchored. The invention also provides immunoglobulin sequences obtainable by the method of the invention. Specifically, the present invention relates to the generation of immunoglobulin sequences by use of DNA vaccination. More specifically, the present invention relates to generation of immunoglobulin sequences in camelids, preferably directed against cell-associated antigens, in particular antigens with multiple transmembrane spanning domains, including GPCRs and ion channels, by DNA vaccination. Furthermore, the present invention relates to said immunoglobulin sequences against cell-associated antigens, more particularly, antigens that are membrane-anchored, such as e.g. GPCRs and ion channels, more preferably ion channels.

摘要翻译： 本发明涉及产生针对细胞相关抗原的免疫球蛋白序列的方法，更具体地说，涉及膜锚定的抗原。 本发明还提供可通过本发明的方法获得的免疫球蛋白序列。 具体地说，本发明涉及使用DNA疫苗接种产生免疫球蛋白序列。 更具体地，本发明涉及通过DNA疫苗接种在骆驼中产生免疫球蛋白序列，优选针对细胞相关抗原，特别是具有多个跨膜跨越结构域的抗原，包括GPCR和离子通道。 此外，本发明涉及针对细胞相关抗原的所述免疫球蛋白序列，更具体地，涉及膜锚定的抗原，例如抗体。 GPCR和离子通道，更优选离子通道。