公开(公告)号：US20050277204A1

公开(公告)日：2005-12-15

申请号：US11056518

申请日：2005-02-11

申请人： Mark Hollis ,  Nicholas Freeland Judson ,  Christina Rudzinski ,  Lalitha Parameswaran ,  Theodore Fedynyshyn ,  Catherine Cabrera ,  Laura Bortolin ,  Robert King ,  Matthew Angel

发明人： Mark Hollis ,  Nicholas Freeland Judson ,  Christina Rudzinski ,  Lalitha Parameswaran ,  Theodore Fedynyshyn ,  Catherine Cabrera ,  Laura Bortolin ,  Robert King ,  Matthew Angel

IPC分类号： C12M1/34 ,  C12Q1/68 ,  C12Q1/70 ,  G01N33/53 ,  G01N33/543

CPC分类号： C12M47/04 ,  G01N33/54326

摘要： The present invention provides improved devices for separating and/or detecting targets from biological, environmental, or chemical samples.

摘要翻译： 本发明提供了用于从生物，环境或化学样品中分离和/或检测靶的改进装置。

公开(公告)号：US20130029418A1

公开(公告)日：2013-01-31

申请号：US13465490

申请日：2012-05-07

申请人： Matthew Angel ,  Christopher Rohde

发明人： Matthew Angel ,  Christopher Rohde

IPC分类号： C12N5/071 ,  C12N5/077 ,  C12N5/078

CPC分类号： C12N5/0696 ,  C12N5/0018 ,  C12N5/0625 ,  C12N5/0647 ,  C12N5/0656 ,  C12N5/0657 ,  C12N5/0676 ,  C12N5/0695 ,  C12N2500/25 ,  C12N2500/33 ,  C12N2500/36 ,  C12N2500/84 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2501/608 ,  C12N2501/998 ,  C12N2506/09 ,  C12N2506/45

摘要： The present invention relates in part to methods for producing tissue-specific cells from patient samples, and to tissue-specific cells produced using these methods. Methods for reprogramming cells using RNA are disclosed. Therapeutics comprising cells produced using these methods are also disclosed.

公开(公告)号：US20140161771A1

公开(公告)日：2014-06-12

申请号：US13903891

申请日：2013-05-28

申请人： Daniel R. Perez ,  Hongjun Chen ,  Yibin Cai ,  Lindomar Jose Pena ,  Matthew Angel

发明人： Daniel R. Perez ,  Hongjun Chen ,  Yibin Cai ,  Lindomar Jose Pena ,  Matthew Angel

IPC分类号： C12N15/86

CPC分类号： C12N15/86 ,  A61K39/12 ,  A61K39/145 ,  A61K2039/525 ,  A61K2039/543 ,  A61K2039/70 ,  C12N7/00 ,  C12N2760/16122 ,  C12N2760/16134 ,  C12N2760/16143 ,  C12N2760/16151 ,  C12N2760/16162

摘要： Disclosed are compositions and methods comprising one or more recombinant influenza viruses. Recombinant influenza viruses with mutated polymerases and/or rearranged genomes are disclosed. Constructs comprising different influenza nucleic acid sequences are also provided. Methods of inducing protecting immunity with the recombinant influenza viruses are disclosed. Also disclosed are methods of plasmid-free production of influenza virus comprising amplicons comprising one or more of influenza genes.

摘要翻译： 公开了包含一种或多种重组流感病毒的组合物和方法。 公开了具有突变聚合酶和/或重排基因组的重组流感病毒。 还提供了包含不同流感核酸序列的构建体。 公开了用重组流感病毒诱导保护免疫的方法。 还公开了无质粒生产包含一种或多种流感基因的扩增子的流感病毒的方法。

公开(公告)号：US08497124B2

公开(公告)日：2013-07-30

申请号：US13465490

申请日：2012-05-07

申请人： Matthew Angel ,  Christopher Rohde

发明人： Matthew Angel ,  Christopher Rohde

IPC分类号： C12N5/071 ,  C12N5/077 ,  C12N5/078

CPC分类号： C12N5/0696 ,  C12N5/0018 ,  C12N5/0625 ,  C12N5/0647 ,  C12N5/0656 ,  C12N5/0657 ,  C12N5/0676 ,  C12N5/0695 ,  C12N2500/25 ,  C12N2500/33 ,  C12N2500/36 ,  C12N2500/84 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2501/608 ,  C12N2501/998 ,  C12N2506/09 ,  C12N2506/45

摘要： The present invention relates in part to methods for producing tissue-specific cells from patient samples, and to tissue-specific cells produced using these methods. Methods for reprogramming cells using RNA are disclosed. Therapeutics comprising cells produced using these methods are also disclosed.

摘要翻译： 本发明部分地涉及从患者样品中产生组织特异性细胞的方法以及使用这些方法产生的组织特异性细胞。 公开了使用RNA重编程细胞的方法。 还公开了包含使用这些方法生产的细胞的治疗剂。

公开(公告)号：US20120208278A1

公开(公告)日：2012-08-16

申请号：US13455327

申请日：2012-04-25

申请人： Mehmet Fatih Yanik ,  Matthew Angel

发明人： Mehmet Fatih Yanik ,  Matthew Angel

IPC分类号： C12N15/85 ,  C12N13/00 ,  C12N5/10 ,  C12N5/071

CPC分类号： C12N15/67 ,  C12N15/113 ,  C12N15/1136 ,  C12N2310/14

摘要： The present invention relates in part to methods for suppressing the innate immune response of a cell to transfection with an exogenous nucleic acid, to methods for increasing expression of a protein encoded by an exogenous nucleic acid by repeated delivery of the exogenous nucleic acid to a cell, and to methods of changing the phenotype of a cell by differentiating, transdifferentiating or dedifferentiating cells by repeatedly delivering one or more nucleic acids that encode defined proteins. A method is provided for extended transient transfection by repeated delivery of an in vitro-transcribed RNA (“ivT-RNA”) to a cell to achieve a high and sustained level of expression of a protein encoded by an ivT-RNA transcripts.

摘要翻译： 本发明部分涉及用外源核酸抑制细胞对转染的先天免疫应答的方法，通过将外源核酸重复递送至细胞来增加由外源核酸编码的蛋白质的表达的方法 以及通过重复递送编码定义的蛋白质的一种或多种核酸来分化，转分化或去分化细胞来改变细胞表型的方法。 提供了通过将体外转录的RNA（“ivT-RNA”）重复递送到细胞以实现由ivT-RNA转录物编码的蛋白质的高且持续水平的表达的延长的瞬时转染的方法。