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公开(公告)号:US5628995A
公开(公告)日:1997-05-13
申请号:US377690
申请日:1995-01-25
申请人: Marnix Peferoen , Stefan Jansens , Peter Denolf
发明人: Marnix Peferoen , Stefan Jansens , Peter Denolf
IPC分类号: A01N63/02 , C07K14/325 , A01N63/00 , A01H4/00 , A61K48/00
CPC分类号: C07K14/325 , A01N63/02
摘要: A method to combat or control Ostrinia nubilalis by contacting such insects with a CryIB protein or a combination of a CryIB protein and a CryIAb or CryIAc protein.
摘要翻译: 通过将这种昆虫与CryIB蛋白或CryIB蛋白和CryIAb或CryIAc蛋白的组合接触来对抗或控制Ostrinia nubilalis的方法。
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公开(公告)号:US5530197A
公开(公告)日:1996-06-25
申请号:US463513
申请日:1995-06-05
申请人: Marnix Peferoen , Stefan Jansens , Peter Denolf
发明人: Marnix Peferoen , Stefan Jansens , Peter Denolf
IPC分类号: A01N63/02 , C07K14/325 , A01H4/00 , A01N63/00
CPC分类号: A01N63/02 , C07K14/325
摘要: A method to combat or control Ostrinia nubilalis by contacting such insects with a CryIB protein or a combination of a CryIB protein and a CryIAb or CryIAc protein.
摘要翻译: 通过将这种昆虫与CryIB蛋白或CryIB蛋白和CryIAb或CryIAc蛋白的组合接触来对抗或控制Ostrinia nubilalis的方法。
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公开(公告)号:US06727409B1
公开(公告)日:2004-04-27
申请号:US09220806
申请日:1998-12-28
IPC分类号: C02K14325
CPC分类号: C12R1/075 , A01N63/00 , C07K14/325 , C12N15/8286 , Y02A40/162
摘要: Four novel Bacillus thuringiensis strains, which are deposited at the BCCM-LMG under accession nos. LMG P-12592, LMG P-12593, LMG P-12594, and LMG P-13493, produce new crystal proteins during sporulation that are toxic to Lepidoptera, more particularly against Noctuidae such as Spodoptera spp. and Agrotis ipsilon, against Pyralidae such as Ostrinta nubilalis, and against Yponomeutidae such as Plutella xylostella, and that are encoded by a novel gene. The crystal proteins contain protoxins, which can yield a toxin as trypsin-digestion product. A plant, the genome of which is transformed with a DNA sequence that comes from either one of the strains and that encodes its respective toxin, is resistant to Lepidoptera. Each strain, itself, or its crystals, crystal proteins, protoxin or toxin can be used as the active ingredient in an insecticidal composition for combatting Lepidoptera.
摘要翻译: 四种新型苏云金芽孢杆菌菌株,其保藏在BCCM-LMG,保藏号为。 LMG P-12592,LMG P-12593,LMG P-12594和LMG P-13493在孢子形成过程中产生对鳞翅目有毒的新的晶体蛋白质,更特别地针对夜蛾科(Spodoptera spp)等等。 和Agrotis ipsilon,针对犀牛科(Ostrinta nubilalis)和针对诸如小菜蛾(Plastella xylostella)的Y科(Yponomeutidae),并由新基因编码。 晶体蛋白含有原毒素,其可以产生作为胰蛋白酶消化产物的毒素。 一种植物,其基因组用来自任一种菌株并编码其各自毒素的DNA序列转化,对鳞翅目具有抗性。 每种菌株本身或其晶体,晶体蛋白质,原毒素或毒素都可用作杀虫鳞虫的杀虫组合物中的活性成分。
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公开(公告)号:US6028246A
公开(公告)日:2000-02-22
申请号:US455838
申请日:1995-05-31
IPC分类号: A01H5/00 , A01M1/20 , A01N63/00 , A01N63/02 , C07K14/325 , C12N1/20 , C12N1/21 , C12N5/04 , C12N5/10 , C12N15/09 , C12N15/32 , C12N15/82 , C12R1/07 , A01H4/00
CPC分类号: C12R1/075 , A01N63/00 , C07K14/325 , C12N15/8286
摘要: Four novel Bacillus thuringiensis strains, which are deposited at the BCCM-LMG under accession nos. LMG P-12592, LMG P-12593, LMG P-12594, and LMG P-13493, produce new crystal proteins during sporulation that are toxic to Lepidoptera, more particularly against Noctuidae such as spodoptera spp. and Agotis ipsilon, against Pyralidae such as Ostrinia nubilalis, and against Yponomeutidae such as Plutella xylostella, and that are encoded by a novel gene. The crystal proteins contain protoxins, which can yield a toxin as trypsin-digestion product. A plant, the genome of which is transformed with a DNA sequence that comes from either one of the strains nd that encodes its respective toxin, is resistant to Lipidoptera. Each strain, itself, or its crystal, crystal protein, protoxin or toxin can be used as the active ingredient in an insecticidal composition for combating Lepidoptera.
摘要翻译: 四种新型苏云金芽孢杆菌菌株,其保藏在BCCM-LMG,保藏号为。 LMG P-12592,LMG P-12593,LMG P-12594和LMG P-13493在孢子形成期间产生对鳞翅目有毒的新的晶体蛋白质,更特别地针对夜蛾科,如草地贪夜蛾。 和Agotis ipsilon,针对鞭毛虫如鸵鸟(Ostrinia nubilalis),以及针对id木科(例如小菜蛾),并且由新基因编码。 晶体蛋白含有原毒素,其可以产生作为胰蛋白酶消化产物的毒素。 用来自编码其各自毒素的一个菌株的DNA序列转化其基因组的植物对脂皮鳞翅目具有抗性。 每种菌株本身或其晶体,晶体蛋白质,原毒素或毒素都可以用作杀虫鳞片中的杀虫组合物中的活性成分。
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公开(公告)号:US20050097635A1
公开(公告)日:2005-05-05
申请号:US10687879
申请日:2003-10-20
IPC分类号: A01H5/00 , A01M1/20 , A01N63/00 , A01N63/02 , C07K14/325 , C12N1/20 , C12N1/21 , C12N5/04 , C12N5/10 , C12N15/09 , C12N15/32 , C12N15/82 , C12R1/07 , A01N65/00 , C07H21/04 , A01H1/00
CPC分类号: C12R1/075 , A01N63/00 , C07K14/325 , C12N15/8286 , Y02A40/162
摘要: Four novel Bacillus thuringiensis strains, which are deposited at the BCCM-LMG under accession nos. LMG P-12592, LMG P-12593, LMG P-12594, and LMG P-13493, produce new crystal proteins during sporulation that are toxic to Lepidoptera, more particularly against Noctuidae such as Spodoptera spp. and Agrotis ipsilon, against Pyralidae such as Ostrinta nubilalis, and against Yponomeutidae such as Plutella xylostella, and that are encoded by a novel gene. The crystal proteins contain protoxins, which can yield a toxin as trypsin-digestion product. A plant, the genome of which is transformed with a DNA sequence that comes from either one of the strains and that encodes its respective toxin, is resistant to Lepidoptera. Each strain, itself, or its crystals, crystal proteins, protoxin or toxin can be used as the active ingredient in an insecticidal composition for combatting Lepidoptera.
摘要翻译: 四种新型苏云金芽孢杆菌菌株,其保藏在BCCM-LMG,保藏号为。 LMG P-12592,LMG P-12593,LMG P-12594和LMG P-13493在孢子形成过程中产生对鳞翅目有毒的新的晶体蛋白质,更特别地针对夜蛾科(Spodoptera spp)等等。 和Agrotis ipsilon,针对犀牛科(Ostrinta nubilalis)和针对诸如小菜蛾(Plastella xylostella)的Y科(Yponomeutidae),并由新基因编码。 晶体蛋白含有原毒素,其可以产生作为胰蛋白酶消化产物的毒素。 一种植物,其基因组用来自任一种菌株并编码其各自毒素的DNA序列转化,对鳞翅目具有抗性。 每种菌株本身或其晶体,晶体蛋白质,原毒素或毒素都可用作杀虫鳞虫的杀虫组合物中的活性成分。
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公开(公告)号:US5659123A
公开(公告)日:1997-08-19
申请号:US295060
申请日:1994-08-26
申请人: Jeroen Van Rie , Stefan Jansens , Marnix Peferoen
发明人: Jeroen Van Rie , Stefan Jansens , Marnix Peferoen
IPC分类号: C07K14/325 , A01H4/00 , C12N15/32
CPC分类号: C07K14/325
摘要: New modified Cry proteins, particularly modified CryIIIA proteins, having significantly altered toxicity, and DNA sequences encoding these proteins, are designed. Analysis of solvent-accessible amino acid positions in domain II of the CryIIIA protein by alanine-scanning mutagenesis identified individual amino acids involved in corn rootworm toxicity. Random replacement of these amino acids identifies modified proteins with improved toxicity. A combination of all or most thus identified improved amino acids in a single protein yields modified CryIIIA proteins with significantly improved toxicity. Particularly regions protruding from the Cry molecule and located at the apex of the Cry proteins, were identified as involved in toxicity.
摘要翻译: 设计了具有显着改变的毒性的新修饰的Cry蛋白,特别是经修饰的CryIIIA蛋白,以及编码这些蛋白质的DNA序列。 通过丙氨酸扫描诱变分析CryIIIA蛋白的结构域II中的溶剂可及氨基酸位置,鉴定了涉及玉米根虫毒性的单个氨基酸。 这些氨基酸的随机替代识别具有改善的毒性的经修饰的蛋白质。 单一蛋白质中所有或大多数如此鉴定的改良氨基酸的组合产生具有显着改善的毒性的改良的CryIIIA蛋白质。 特别是从Cry分子突出并位于Cry蛋白的顶点的区域被鉴定为涉及毒性。
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公开(公告)号:US07361751B2
公开(公告)日:2008-04-22
申请号:US10687879
申请日:2003-10-20
IPC分类号: C12N15/32 , C07K14/325
CPC分类号: C12R1/075 , A01N63/00 , C07K14/325 , C12N15/8286 , Y02A40/162
摘要: Four novel Bacillus thuringiensis strains, which are deposited at the BCCM-LMG under accession nos. LMG P-12592, LMG P-12593, LMG P-12594, and LMG P-13493, produce new crystal proteins during sporulation that are toxic to Lepidoptera, more particularly against Noctuidae such as Spodoptera spp. and Agrotis ipsilon, against Pyralidae such as Ostrinta nubilalis, and against Yponomeutidae such as Plutella xylostella, and that are encoded by a novel gene. The crystal proteins contain protoxins, which can yield a toxin as trypsin-digestion product. A plant, the genome of which is transformed with a DNA sequence that comes from either one of the strains and that encodes its respective toxin, is resistant to Lepidoptera. Each strain, itself, or its crystals, crystal proteins, protoxin or toxin can be used as the active ingredient in an insecticidal composition for combatting Lepidoptera.
摘要翻译: 四种新型苏云金芽孢杆菌菌株,其保藏在BCCM-LMG,保藏号为。 LMG P-12592,LMG P-12593,LMG P-12594和LMG P-13493在孢子形成期间产生对鳞翅目有毒的新的晶体蛋白质,更特别地针对夜蛾科(Spodoptera spp)等等。 和Agrotis ipsilon,针对犀牛科(Ostrinta nubilalis)和针对诸如小菜蛾(Plastella xylostella)的Y科(Yponomeutidae),并由新基因编码。 晶体蛋白含有原毒素,其可以产生作为胰蛋白酶消化产物的毒素。 一种植物,其基因组用来自任一种菌株并编码其各自毒素的DNA序列转化,对鳞翅目具有抗性。 每种菌株本身或其晶体,晶体蛋白质,原毒素或毒素都可用作杀虫鳞虫的杀虫组合物中的活性成分。
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公开(公告)号:US06448226B1
公开(公告)日:2002-09-10
申请号:US09471177
申请日:1999-12-23
申请人: Bart Lambert , Stefan Jansens , Katrien Van Audenhove , Marnix Peferoen , Jeroen Van Rie , Roel Van Aarssen
发明人: Bart Lambert , Stefan Jansens , Katrien Van Audenhove , Marnix Peferoen , Jeroen Van Rie , Roel Van Aarssen
IPC分类号: A01N3718
CPC分类号: C12R1/075 , A01N63/00 , C07K14/325 , C12N15/8286 , Y02A40/162 , Y10S424/832 , Y10S530/825
摘要: The present invention relates to transformed plants and plant cells comprising DNA molecules encoding Bacillus thuringiensis proteins with insecticidal activity. The invention relates more particularly to transformed plants and plant cells comprising DNA molecules encoding the protease resistant toxins BTS02618Aa or BTS02618Ab, as well as to methods of rendering plants or plant cells resistant to insects using these DNA molecules.
摘要翻译: 本发明涉及包含编码具有杀虫活性的苏云金芽孢杆菌蛋白质的DNA分子的转化植物和植物细胞。 本发明更具体地涉及包含编码抗蛋白酶抗性毒素BTS02618Aa或BTS02618Ab的DNA分子的转化植物和植物细胞,以及使用这些DNA分子提供对昆虫具有抗性的植物或植物细胞的方法。
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公开(公告)号:US07888471B2
公开(公告)日:2011-02-15
申请号:US12076204
申请日:2008-03-14
IPC分类号: C07K14/325 , C12N15/35
CPC分类号: C12R1/075 , A01N63/00 , C07K14/325 , C12N15/8286 , Y02A40/162
摘要: Four novel Bacillus thuringiensis strains, which are deposited at the BCCM-LMG under accession nos. LMG P-12592, LMG P-12593, LMG P-12594, and LMG P-13493, produce new crystal proteins during sporulation that are toxic to Lepidoptera, more particularly against Noctuidae such as Spodoptera spp. and Agrotis ipsilon, against Pyralidae such as Ostrinta nubilalis, and against Yponomeutidae such as Plutella xylostella, and that are encoded by a novel gene. The crystal proteins contain protoxins, which can yield a toxin as trypsin-digestion product. A plant, the genome of which is transformed with a DNA sequence that comes from either one of the strains and that encodes its respective toxin, is resistant to Lepidoptera. Each strain, itself, or its crystals, crystal proteins, protoxin or toxin can be used as the active ingredient in an insecticidal composition for combatting Lepidoptera.
摘要翻译: 四种新型苏云金芽孢杆菌菌株,其保藏在BCCM-LMG,保藏号为。 LMG P-12592,LMG P-12593,LMG P-12594和LMG P-13493在孢子形成过程中产生对鳞翅目有毒的新的晶体蛋白质,更特别地针对夜蛾科(Spodoptera spp)等等。 和Agrotis ipsilon,针对犀牛科(Ostrinta nubilalis)和针对诸如小菜蛾(Plastella xylostella)的Y科(Yponomeutidae),并由新基因编码。 晶体蛋白含有原毒素,其可以产生作为胰蛋白酶消化产物的毒素。 一种植物,其基因组用来自任一种菌株并编码其各自毒素的DNA序列转化,对鳞翅目具有抗性。 每种菌株本身或其晶体,晶体蛋白质,原毒素或毒素都可用作杀虫鳞虫的杀虫组合物中的活性成分。
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10.
公开(公告)号:US20090192290A1
公开(公告)日:2009-07-30
申请号:US12076204
申请日:2008-03-14
IPC分类号: C07K14/00
CPC分类号: C12R1/075 , A01N63/00 , C07K14/325 , C12N15/8286 , Y02A40/162
摘要: Four novel Bacillus thuringiensis strains, which are deposited at the BCCM-LMG under accession nos. LMG P-12592, LMG P-12593, LMG P-12594, and LMG P-13493, produce new crystal proteins during sporulation that are toxic to Lepidoptera, more particularly against Noctuidae such as Spodoptera spp. and Agrotis ipsilon, against Pyralidae such as Ostrinta nubilalis, and against Yponomeutidae such as Plutella xylostella, and that are encoded by a novel gene. The crystal proteins contain protoxins, which can yield a toxin as trypsin-digestion product. A plant, the genome of which is transformed with a DNA sequence that comes from either one of the strains and that encodes its respective toxin, is resistant to Lepidoptera. Each strain, itself, or its crystals, crystal proteins, protoxin or toxin can be used as the active ingredient in an insecticidal composition for combatting Lepidoptera.
摘要翻译: 四种新型苏云金芽孢杆菌菌株,其保藏在BCCM-LMG,保藏号为。 LMG P-12592,LMG P-12593,LMG P-12594和LMG P-13493在孢子形成过程中产生对鳞翅目有毒的新的晶体蛋白质,更特别地针对夜蛾科(Spodoptera spp)等等。 和Agrotis ipsilon,针对犀牛科(Ostrinta nubilalis)和针对诸如小菜蛾(Plastella xylostella)的Y科(Yponomeutidae),并由新基因编码。 晶体蛋白含有原毒素,其可以产生作为胰蛋白酶消化产物的毒素。 一种植物,其基因组用来自任一种菌株并编码其各自毒素的DNA序列转化,对鳞翅目具有抗性。 每种菌株本身或其晶体,晶体蛋白质,原毒素或毒素都可用作杀虫鳞虫的杀虫组合物中的活性成分。
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