Bacillus thuringiensis strains and their insecticidal proteins
    3.
    发明授权
    Bacillus thuringiensis strains and their insecticidal proteins 失效
    苏云金芽孢杆菌菌株及其杀虫蛋白

    公开(公告)号:US06727409B1

    公开(公告)日:2004-04-27

    申请号:US09220806

    申请日:1998-12-28

    IPC分类号: C02K14325

    摘要: Four novel Bacillus thuringiensis strains, which are deposited at the BCCM-LMG under accession nos. LMG P-12592, LMG P-12593, LMG P-12594, and LMG P-13493, produce new crystal proteins during sporulation that are toxic to Lepidoptera, more particularly against Noctuidae such as Spodoptera spp. and Agrotis ipsilon, against Pyralidae such as Ostrinta nubilalis, and against Yponomeutidae such as Plutella xylostella, and that are encoded by a novel gene. The crystal proteins contain protoxins, which can yield a toxin as trypsin-digestion product. A plant, the genome of which is transformed with a DNA sequence that comes from either one of the strains and that encodes its respective toxin, is resistant to Lepidoptera. Each strain, itself, or its crystals, crystal proteins, protoxin or toxin can be used as the active ingredient in an insecticidal composition for combatting Lepidoptera.

    摘要翻译: 四种新型苏云金芽孢杆菌菌株,其保藏在BCCM-LMG,保藏号为。 LMG P-12592,LMG P-12593,LMG P-12594和LMG P-13493在孢子形成过程中产生对鳞翅目有毒的新的晶体蛋白质,更特别地针对夜蛾科(Spodoptera spp)等等。 和Agrotis ipsilon,针对犀牛科(Ostrinta nubilalis)和针对诸如小菜蛾(Plastella xylostella)的Y科(Yponomeutidae),并由新基因编码。 晶体蛋白含有原毒素,其可以产生作为胰蛋白酶消化产物的毒素。 一种植物,其基因组用来自任一种菌株并编码其各自毒素的DNA序列转化,对鳞翅目具有抗性。 每种菌株本身或其晶体,晶体蛋白质,原毒素或毒素都可用作杀虫鳞虫的杀虫组合物中的活性成分。

    Bacillus thuringiensis strains and their insecticidal proteins
    4.
    发明授权
    Bacillus thuringiensis strains and their insecticidal proteins 失效
    苏云金芽孢杆菌菌株及其杀虫蛋白

    公开(公告)号:US6028246A

    公开(公告)日:2000-02-22

    申请号:US455838

    申请日:1995-05-31

    摘要: Four novel Bacillus thuringiensis strains, which are deposited at the BCCM-LMG under accession nos. LMG P-12592, LMG P-12593, LMG P-12594, and LMG P-13493, produce new crystal proteins during sporulation that are toxic to Lepidoptera, more particularly against Noctuidae such as spodoptera spp. and Agotis ipsilon, against Pyralidae such as Ostrinia nubilalis, and against Yponomeutidae such as Plutella xylostella, and that are encoded by a novel gene. The crystal proteins contain protoxins, which can yield a toxin as trypsin-digestion product. A plant, the genome of which is transformed with a DNA sequence that comes from either one of the strains nd that encodes its respective toxin, is resistant to Lipidoptera. Each strain, itself, or its crystal, crystal protein, protoxin or toxin can be used as the active ingredient in an insecticidal composition for combating Lepidoptera.

    摘要翻译: 四种新型苏云金芽孢杆菌菌株,其保藏在BCCM-LMG,保藏号为。 LMG P-12592,LMG P-12593,LMG P-12594和LMG P-13493在孢子形成期间产生对鳞翅目有毒的新的晶体蛋白质,更特别地针对夜蛾科,如草地贪夜蛾。 和Agotis ipsilon,针对鞭毛虫如鸵鸟(Ostrinia nubilalis),以及针对id木科(例如小菜蛾),并且由新基因编码。 晶体蛋白含有原毒素,其可以产生作为胰蛋白酶消化产物的毒素。 用来自编码其各自毒素的一个菌株的DNA序列转化其基因组的植物对脂皮鳞翅目具有抗性。 每种菌株本身或其晶体,晶体蛋白质,原毒素或毒素都可以用作杀虫鳞片中的杀虫组合物中的活性成分。

    Bacillus thuringiensis strains and their insecticidal proteins
    5.
    发明申请
    Bacillus thuringiensis strains and their insecticidal proteins 失效
    苏云金芽孢杆菌菌株及其杀虫蛋白

    公开(公告)号:US20050097635A1

    公开(公告)日:2005-05-05

    申请号:US10687879

    申请日:2003-10-20

    摘要: Four novel Bacillus thuringiensis strains, which are deposited at the BCCM-LMG under accession nos. LMG P-12592, LMG P-12593, LMG P-12594, and LMG P-13493, produce new crystal proteins during sporulation that are toxic to Lepidoptera, more particularly against Noctuidae such as Spodoptera spp. and Agrotis ipsilon, against Pyralidae such as Ostrinta nubilalis, and against Yponomeutidae such as Plutella xylostella, and that are encoded by a novel gene. The crystal proteins contain protoxins, which can yield a toxin as trypsin-digestion product. A plant, the genome of which is transformed with a DNA sequence that comes from either one of the strains and that encodes its respective toxin, is resistant to Lepidoptera. Each strain, itself, or its crystals, crystal proteins, protoxin or toxin can be used as the active ingredient in an insecticidal composition for combatting Lepidoptera.

    摘要翻译: 四种新型苏云金芽孢杆菌菌株,其保藏在BCCM-LMG,保藏号为。 LMG P-12592,LMG P-12593,LMG P-12594和LMG P-13493在孢子形成过程中产生对鳞翅目有毒的新的晶体蛋白质,更特别地针对夜蛾科(Spodoptera spp)等等。 和Agrotis ipsilon,针对犀牛科(Ostrinta nubilalis)和针对诸如小菜蛾(Plastella xylostella)的Y科(Yponomeutidae),并由新基因编码。 晶体蛋白含有原毒素,其可以产生作为胰蛋白酶消化产物的毒素。 一种植物,其基因组用来自任一种菌株并编码其各自毒素的DNA序列转化,对鳞翅目具有抗性。 每种菌株本身或其晶体,晶体蛋白质,原毒素或毒素都可用作杀虫鳞虫的杀虫组合物中的活性成分。

    Diabrotica toxins
    6.
    发明授权
    Diabrotica toxins 失效
    赤霉素毒素

    公开(公告)号:US5659123A

    公开(公告)日:1997-08-19

    申请号:US295060

    申请日:1994-08-26

    IPC分类号: C07K14/325 A01H4/00 C12N15/32

    CPC分类号: C07K14/325

    摘要: New modified Cry proteins, particularly modified CryIIIA proteins, having significantly altered toxicity, and DNA sequences encoding these proteins, are designed. Analysis of solvent-accessible amino acid positions in domain II of the CryIIIA protein by alanine-scanning mutagenesis identified individual amino acids involved in corn rootworm toxicity. Random replacement of these amino acids identifies modified proteins with improved toxicity. A combination of all or most thus identified improved amino acids in a single protein yields modified CryIIIA proteins with significantly improved toxicity. Particularly regions protruding from the Cry molecule and located at the apex of the Cry proteins, were identified as involved in toxicity.

    摘要翻译: 设计了具有显着改变的毒性的新修饰的Cry蛋白,特别是经修饰的CryIIIA蛋白,以及编码这些蛋白质的DNA序列。 通过丙氨酸扫描诱变分析CryIIIA蛋白的结构域II中的溶剂可及氨基酸位置,鉴定了涉及玉米根虫毒性的单个氨基酸。 这些氨基酸的随机替代识别具有改善的毒性的经修饰的蛋白质。 单一蛋白质中所有或大多数如此鉴定的改良氨基酸的组合产生具有显着改善的毒性的改良的CryIIIA蛋白质。 特别是从Cry分子突出并位于Cry蛋白的顶点的区域被鉴定为涉及毒性。

    Bacillus thuringiensis strains and their insecticidal proteins
    7.
    发明授权
    Bacillus thuringiensis strains and their insecticidal proteins 失效
    苏云金芽孢杆菌菌株及其杀虫蛋白

    公开(公告)号:US07361751B2

    公开(公告)日:2008-04-22

    申请号:US10687879

    申请日:2003-10-20

    IPC分类号: C12N15/32 C07K14/325

    摘要: Four novel Bacillus thuringiensis strains, which are deposited at the BCCM-LMG under accession nos. LMG P-12592, LMG P-12593, LMG P-12594, and LMG P-13493, produce new crystal proteins during sporulation that are toxic to Lepidoptera, more particularly against Noctuidae such as Spodoptera spp. and Agrotis ipsilon, against Pyralidae such as Ostrinta nubilalis, and against Yponomeutidae such as Plutella xylostella, and that are encoded by a novel gene. The crystal proteins contain protoxins, which can yield a toxin as trypsin-digestion product. A plant, the genome of which is transformed with a DNA sequence that comes from either one of the strains and that encodes its respective toxin, is resistant to Lepidoptera. Each strain, itself, or its crystals, crystal proteins, protoxin or toxin can be used as the active ingredient in an insecticidal composition for combatting Lepidoptera.

    摘要翻译: 四种新型苏云金芽孢杆菌菌株,其保藏在BCCM-LMG,保藏号为。 LMG P-12592,LMG P-12593,LMG P-12594和LMG P-13493在孢子形成期间产生对鳞翅目有毒的新的晶体蛋白质,更特别地针对夜蛾科(Spodoptera spp)等等。 和Agrotis ipsilon,针对犀牛科(Ostrinta nubilalis)和针对诸如小菜蛾(Plastella xylostella)的Y科(Yponomeutidae),并由新基因编码。 晶体蛋白含有原毒素,其可以产生作为胰蛋白酶消化产物的毒素。 一种植物,其基因组用来自任一种菌株并编码其各自毒素的DNA序列转化,对鳞翅目具有抗性。 每种菌株本身或其晶体,晶体蛋白质,原毒素或毒素都可用作杀虫鳞虫的杀虫组合物中的活性成分。

    Bacillus thuringiensis strains and their insecticidal proteins
    9.
    发明授权
    Bacillus thuringiensis strains and their insecticidal proteins 失效
    苏云金芽孢杆菌菌株及其杀虫蛋白

    公开(公告)号:US07888471B2

    公开(公告)日:2011-02-15

    申请号:US12076204

    申请日:2008-03-14

    IPC分类号: C07K14/325 C12N15/35

    摘要: Four novel Bacillus thuringiensis strains, which are deposited at the BCCM-LMG under accession nos. LMG P-12592, LMG P-12593, LMG P-12594, and LMG P-13493, produce new crystal proteins during sporulation that are toxic to Lepidoptera, more particularly against Noctuidae such as Spodoptera spp. and Agrotis ipsilon, against Pyralidae such as Ostrinta nubilalis, and against Yponomeutidae such as Plutella xylostella, and that are encoded by a novel gene. The crystal proteins contain protoxins, which can yield a toxin as trypsin-digestion product. A plant, the genome of which is transformed with a DNA sequence that comes from either one of the strains and that encodes its respective toxin, is resistant to Lepidoptera. Each strain, itself, or its crystals, crystal proteins, protoxin or toxin can be used as the active ingredient in an insecticidal composition for combatting Lepidoptera.

    摘要翻译: 四种新型苏云金芽孢杆菌菌株,其保藏在BCCM-LMG,保藏号为。 LMG P-12592,LMG P-12593,LMG P-12594和LMG P-13493在孢子形成过程中产生对鳞翅目有毒的新的晶体蛋白质,更特别地针对夜蛾科(Spodoptera spp)等等。 和Agrotis ipsilon,针对犀牛科(Ostrinta nubilalis)和针对诸如小菜蛾(Plastella xylostella)的Y科(Yponomeutidae),并由新基因编码。 晶体蛋白含有原毒素,其可以产生作为胰蛋白酶消化产物的毒素。 一种植物,其基因组用来自任一种菌株并编码其各自毒素的DNA序列转化,对鳞翅目具有抗性。 每种菌株本身或其晶体,晶体蛋白质,原毒素或毒素都可用作杀虫鳞虫的杀虫组合物中的活性成分。

    New bacillus thuringiensis strains and their insecticidal proteins
    10.
    发明申请
    New bacillus thuringiensis strains and their insecticidal proteins 失效
    新型苏云金芽孢杆菌菌株及其杀虫蛋白

    公开(公告)号:US20090192290A1

    公开(公告)日:2009-07-30

    申请号:US12076204

    申请日:2008-03-14

    IPC分类号: C07K14/00

    摘要: Four novel Bacillus thuringiensis strains, which are deposited at the BCCM-LMG under accession nos. LMG P-12592, LMG P-12593, LMG P-12594, and LMG P-13493, produce new crystal proteins during sporulation that are toxic to Lepidoptera, more particularly against Noctuidae such as Spodoptera spp. and Agrotis ipsilon, against Pyralidae such as Ostrinta nubilalis, and against Yponomeutidae such as Plutella xylostella, and that are encoded by a novel gene. The crystal proteins contain protoxins, which can yield a toxin as trypsin-digestion product. A plant, the genome of which is transformed with a DNA sequence that comes from either one of the strains and that encodes its respective toxin, is resistant to Lepidoptera. Each strain, itself, or its crystals, crystal proteins, protoxin or toxin can be used as the active ingredient in an insecticidal composition for combatting Lepidoptera.

    摘要翻译: 四种新型苏云金芽孢杆菌菌株,其保藏在BCCM-LMG,保藏号为。 LMG P-12592,LMG P-12593,LMG P-12594和LMG P-13493在孢子形成过程中产生对鳞翅目有毒的新的晶体蛋白质,更特别地针对夜蛾科(Spodoptera spp)等等。 和Agrotis ipsilon,针对犀牛科(Ostrinta nubilalis)和针对诸如小菜蛾(Plastella xylostella)的Y科(Yponomeutidae),并由新基因编码。 晶体蛋白含有原毒素,其可以产生作为胰蛋白酶消化产物的毒素。 一种植物,其基因组用来自任一种菌株并编码其各自毒素的DNA序列转化,对鳞翅目具有抗性。 每种菌株本身或其晶体,晶体蛋白质,原毒素或毒素都可用作杀虫鳞虫的杀虫组合物中的活性成分。