公开(公告)号：US08440619B2

公开(公告)日：2013-05-14

申请号：US12065452

申请日：2006-08-31

申请人： Masaaki Tokuda ,  Shoji Kimura ,  Ken Izumori

发明人： Masaaki Tokuda ,  Shoji Kimura ,  Ken Izumori

IPC分类号： A61K38/00 ,  A61K9/12 ,  A61K31/70 ,  A61P9/00

CPC分类号： A23K20/163 ,  A23L33/10 ,  A61K31/7004

摘要： The object is to provide a novel application of D-allose (e.g., use for prevention of hypertension or hypercardia). Thus, disclosed is a composition for preventing the increase in blood pressure which comprises D-allose as an active ingredient; or a composition containing D-allose and/or a derivative thereof, preferably in an amount of 0.1 to 50% by weight. Preferably, the composition is in a form selected from the group consisting of a food additive, a food material, a beverage/food, a health beverage/food, a pharmaceutical and a feeding stuff which can be used for the prevention and treatment of a cardiovascular system disorder (e.g., hypertension, hypercardia). The increase in blood pressure may be caused by salt-sensitive hypertension. Also disclosed is use of D-allose for preventing the increase in blood pressure (excluding medical practices).

摘要翻译： 本发明的目的是提供D-丙氨酸的新应用（例如，用于预防高血压或高血压）。 因此，公开了用于防止血压升高的组合物，其包含D-阿洛糖作为活性成分; 或含有D-丙氨酸和/或其衍生物的组合物，优选为0.1〜50重量％。 优选地，组合物为选自食品添加剂，食品材料，饮料/食品，保健饮料/食品，药物和饲料的形式，其可用于预防和治疗 心血管系统疾病（如高血压，高血压）。 血压升高可能是盐敏感性高血压引起的。 还公开了使用D-丙氨酸来防止血压升高（不包括医疗实践）。

公开(公告)号：US20090305999A1

公开(公告)日：2009-12-10

申请号：US12065452

申请日：2006-08-31

申请人： Masaaki Tokuda ,  Shoji Kimura ,  Ken Izumori

发明人： Masaaki Tokuda ,  Shoji Kimura ,  Ken Izumori

IPC分类号： A61K31/7004 ,  A61P9/12

CPC分类号： A23K20/163 ,  A23L33/10 ,  A61K31/7004

摘要： The object is to provide a novel application of D-allose (e.g., use for prevention of hypertension or hypercardia). Thus, disclosed is a composition for preventing the increase in blood pressure which comprises D-allose as an active ingredient; or a composition containing D-allose and/or a derivative thereof, preferably in an amount of 0.1 to 50% by weight. Preferably, the composition is in a form selected from the group consisting of a food additive, a food material, a beverage/food, a health beverage/food, a pharmaceutical and a feeding stuff which can be used for the prevention and treatment of a cardiovascular system disorder (e.g., hypertension, hypercardia). The increase in blood pressure may be caused by salt-sensitive hypertension. Also disclosed is use of D-allose for preventing the increase in blood pressure (excluding medical practices).

摘要翻译： 本发明的目的是提供D-丙氨酸的新颖应用（例如，用于预防高血压或高血压）。 因此，公开了用于防止血压升高的组合物，其包含D-阿洛糖作为活性成分; 或含有D-丙氨酸和/或其衍生物的组合物，优选为0.1-50重量％。 优选地，组合物为选自食品添加剂，食品材料，饮料/食品，保健饮料/食品，药物和饲料的形式，其可用于预防和治疗 心血管系统疾病（如高血压，高血压）。 血压升高可能是盐敏感性高血压引起的。 还公开了使用D-丙氨酸来防止血压升高（不包括医疗实践）。

公开(公告)号：US20090325231A1

公开(公告)日：2009-12-31

申请号：US12515596

申请日：2007-03-26

申请人： Ken Izumori ,  Kenji Morimoto ,  Goro Takata ,  Masaaki Tokuda ,  Yoshio Tsujisaka ,  Kei Takeshita ,  Keiji Tsusaki ,  Kazuhiro Okuma

发明人： Ken Izumori ,  Kenji Morimoto ,  Goro Takata ,  Masaaki Tokuda ,  Yoshio Tsujisaka ,  Kei Takeshita ,  Keiji Tsusaki ,  Kazuhiro Okuma

IPC分类号： C12P21/00 ,  C12N9/90 ,  C12N1/21 ,  C12P19/24

CPC分类号： C12N9/90 ,  C12P19/24 ,  C12R1/01

摘要： Object: To provide a thermostable L-ribose isomerase.Means for Resolution: The thermostable L-ribose isomerase with MW. 32,000 (by SDS-PAGE), optimal temperature of 45° C., optimal pH of pH 9.0 (glycine-NaOH buffer), and stable physicochemical properties such as temperature stability up to 45° C. during thermal treatment at pH 9.0 for 10 minutes, and with an action to isomerize L-ribose to generate L-ribulose or of inversely to isomerize L-ribulose to generate L-ribose. A conversion method between an aldose and a ketose comprising allowing the thermostable L-ribose isomerase as an enzyme derived from (1) Raoultella ornithinolytica strain MB426 (NITE BP-277) to interact with an aldose selected from L-ribose, D-lyxose, D-tallose, D-mannose, L-allose and L-gulose to isomerize the aldose to generate a ketose selected from the individually corresponding L-ribulose, D-xylulose, D-tagatose, D-fructose, L-psicose and L-sorbose or to interact with a ketose selected from L-ribulose, D-xylulose, D-tagatose, D-fructose, L-psicose and L-sorbose to isomerize the ketose to generate an aldose selected from the individually corresponding L-ribose, D-lyxose, D-tallose, D-mannose, L-allose and L-gulose.

摘要翻译： 目的：提供热稳定的L-核糖异构酶。 解决方法：具有MW的热稳定的L-核糖异构酶。 32,000（通过SDS-PAGE），最适温度为45℃，pH 9.0的最佳pH（甘氨酸 - NaOH缓冲液），以及稳定的物理化学性质，例如在pH 9.0的热处理期间温度稳定性高达45℃，为10 分钟，并且具有异构化L-核糖以产生L-核酮糖的作用或者与L-核酮糖异构化以产生L-核糖的作用。 醛糖和酮糖之间的转化方法，包括使热稳定的L-核糖异构酶作为源自（1）鸟氨酸解脂耶氏杆菌菌株MB426（NITE BP-277）的酶与选自L-核糖，D-赖索糖， D-山梨糖醇，D-甘露糖，L-丙氨酸和L-古洛糖醇以使醛糖异构化以产生选自单独对应的L-核酮糖，D-木酮糖，D-塔格糖，D-果糖，L-灵芝蛋白和L- 山梨糖或与选自L-核酮糖，D-木酮糖，D-塔格糖，D-果糖，L-灵敏糖和L-山梨糖的酮糖相互作用以使酮糖异构化以产生选自相应的L-核糖，D D-果糖，D-甘露糖，D-甘露糖，L-阿洛糖和L-古洛糖。

公开(公告)号：US07501267B2

公开(公告)日：2009-03-10

申请号：US11714887

申请日：2007-03-07

申请人： Ken Izumori ,  Goro Takada ,  Masaaki Tokuda

发明人： Ken Izumori ,  Goro Takada ,  Masaaki Tokuda

IPC分类号： C12P21/06 ,  C07H21/04 ,  C12N1/21 ,  C12N9/90

CPC分类号： C12N9/90 ,  C12P19/02 ,  C12P19/24 ,  C12Y503/01014

摘要： In the rare sugar strategy of Izumoring (FIG. 1), it is intended to establish a reaction system of producing rare sugars of many types by acquiring an isomerase which acts on various rare aldoses and, therefore, is most efficient in producing various rare ketoses. A DNA encoding the following protein (a) or (b). The above-mentioned DNA which is L-rhamnose isomerase derived from Pseudomonas stutzerii. A protein comprising the amino acid sequence represented by SEQ ID NO:2. A process for producing a recombinant protein characterized by culturing a host cell containing an expression system that can express the above-mentioned protein in a medium and collecting a recombinant protein having an L-rhamnose isomerase activity from the thus obtained culture. A method of applying FIG. 1 to the production of a rare sugar characterized in that the location of a target rare sugar in the overall picture of monosaccharides is understood and thus the optimum production pathway on which the above protein is allowed to act is designed.

摘要翻译： 在采食稀少糖的策略（图1）中，旨在通过获得作用于各种罕见的醛糖的异构酶来建立许多类型的稀有糖的反应体系，因此在生产各种稀有酮糖中是最有效的 。 编码以下蛋白质（a）或（b）的DNA。 上述提供的源自番茄假单胞菌的L-鼠李糖异构酶的DNA。 包含SEQ ID NO：2所示的氨基酸序列的蛋白质。 一种重组蛋白质的制造方法，其特征在于，在培养基中培养含有能够在培养基中表达上述蛋白质的表达系统的宿主细胞，从所得培养物中收集具有L-鼠李糖异构酶活性的重组蛋白质。 一种应用图1的方法。 1涉及生产一种罕见的糖，其特征在于，在单糖的总体图中，目标稀有糖的位置被理解，因此设计了允许上述蛋白质起作用的最佳生产途径。

公开(公告)号：US20060148061A1

公开(公告)日：2006-07-06

申请号：US10541822

申请日：2004-01-09

申请人： Ken Izumori ,  Goro Takada ,  Masaaki Tokuda

发明人： Ken Izumori ,  Goro Takada ,  Masaaki Tokuda

IPC分类号： C07H21/04 ,  C12P21/06 ,  C12P19/02 ,  C12N9/90 ,  C12N15/74 ,  C12N1/21

CPC分类号： C12N9/90 ,  C12P19/02 ,  C12P19/24 ,  C12Y503/01014

摘要： In the rare sugar strategy of Izumoring (FIG. 1), it is intended to establish a reaction system of producing rare sugars of many types by acquiring an isomerase which acts on various rare aldoses and, therefore, is most efficient in producing various rare ketoses. A DNA encoding the following protein (a) or (b). The above-mentioned DNA which is L-rhamnose isomerase derived from Pseudomonas stutzerii. A protein comprising the amino acid sequence represented by SEQ ID NO:2. A process for producing a recombinant protein characterized by culturing a host cell containing an expression system that can express the above-mentioned protein in a medium and collecting a recombinant protein having an L-rhamnose isomerase activity from the thus obtained culture. A method of applying FIG. 1 to the production of a rare sugar characterized in that the location of a target rare sugar in the overall picture of monosaccharides is understood and thus the optimum production pathway on which the above protein is allowed to act is designed.

摘要翻译： 在采食稀少糖的策略（图1）中，旨在通过获得作用于各种罕见的醛糖的异构酶来建立许多类型的稀有糖的反应体系，因此在生产各种稀有酮糖中是最有效的 。 编码以下蛋白质（a）或（b）的DNA。 上述提供的源自番茄假单胞菌的L-鼠李糖异构酶的DNA。 包含SEQ ID NO：2所示的氨基酸序列的蛋白质。 一种重组蛋白质的制造方法，其特征在于，在培养基中培养含有能够在培养基中表达上述蛋白质的表达系统的宿主细胞，从所得培养物中收集具有L-鼠李糖异构酶活性的重组蛋白质。 一种应用图1的方法。 1涉及生产一种罕见的糖，其特征在于，在单糖的总体图中，目标稀有糖的位置被理解，因此设计了允许上述蛋白质起作用的最佳生产途径。

公开(公告)号：US09237744B2

公开(公告)日：2016-01-19

申请号：US11569577

申请日：2005-05-26

申请人： Masaaki Tokuda ,  Masaaki Ueki ,  Rikiya Taoka ,  Yoshiyuki Kakehi ,  Ken Izumori

发明人： Masaaki Tokuda ,  Masaaki Ueki ,  Rikiya Taoka ,  Yoshiyuki Kakehi ,  Ken Izumori

IPC分类号： A01N1/02

CPC分类号： A01N1/0221 ,  A01N1/02

摘要： A preservative solution for low temperature preservation of animal or human organs and animal or plant tissues or cells contains D-allose. For the human kidney, cells derived from animals or humans, or human or animal sperm and/or ovum and/or fermented ovum, the preservation is conducted at from −5 to 20° C. For cells derived from animals or humans, or human or animal sperm and/or ovum and/or fermented ovum, the preservation is conducted at a temperature at which to start freezing to −196° C. A method for the low temperature preservation of an animal or human organ and an animal or plant tissue or cell at a low temperature comprises (a) refluxing or immersing a preservative solution containing D-allose and the organ or the tissue or mixing the cell therewith, and (b) cooling the organ, the tissue or the cell to a low temperature of from −5 to 20° C.

摘要翻译： 用于动物或人体器官以及动物或植物组织或细胞的低温保存的防腐剂溶液含有D-丙氨酸。 对于人肾脏，来自动物或人类的细胞，或人或动物精子和/或卵子和/或发酵的卵子，在-5至20℃进行保存。对于来自动物或人类或人类的细胞 或动物精子和/或卵子和/或发酵的卵子，在开始冷冻至-196℃的温度下进行保存。用于动物或人体器官以及动物或植物组织的低温保存的方法 或细胞在低温下包括（a）回流或浸入含有D-丙氨酸和器官或组织的防腐溶液或混合细胞，和（b）将器官，组织或细胞冷却至低温 -5〜20℃

公开(公告)号：US08530222B2

公开(公告)日：2013-09-10

申请号：US12515602

申请日：2007-03-27

申请人： Ken Izumori ,  Kenji Morimoto ,  Goro Takata ,  Masaaki Tokuda ,  Yoshio Tsujisaka ,  Kei Takeshita ,  Keiji Tsusaki ,  Kazuhiro Okuma

发明人： Ken Izumori ,  Kenji Morimoto ,  Goro Takata ,  Masaaki Tokuda ,  Yoshio Tsujisaka ,  Kei Takeshita ,  Keiji Tsusaki ,  Kazuhiro Okuma

IPC分类号： C12N1/20

CPC分类号： C12R1/01 ,  A61K8/60 ,  A61K2800/86 ,  A61Q19/00 ,  C12N9/0006 ,  C12P19/02

摘要： Problem: To provide a microorganism with an ability to produce deoxy polyol dehydrogenase.Means for Resolution: A microorganism belonging to genus Enterobacter with an ability to produce a dehydrogenase for deoxy polyol of the same structure at the positions C2 and C3 as that of ribitol or L-iditol. The bacterial cell IK7 of the genus Enterobacter (accession No. NITE P-271). A method for producing deoxy ketose comprising allowing a culture containing the deoxy polyol dehydrogenase obtained by the culturing of the microorganism of the invention or allowing the deoxy polyol dehydrogenase to react with a solution containing deoxy polyol of the same structure at the positions C2 and C3 as that of ribitol or L-iditol to oxidize deoxy polyol to produce the corresponding deoxy ketose and then collecting the deoxy ketose. The deoxy polyol is 1-deoxy-D-allitol, while the corresponding deoxy ketose is 1-deoxy-L-psicose. Otherwise, the deoxy polyol is L-rhamnitol, while the corresponding deoxy ketose is 1-deoxy-L-fructose.

摘要翻译： 问题：提供具有生产脱氧多元醇脱氢酶能力的微生物。 解决方法：属于肠杆菌属的微生物，其在位置C2和C3处产生与核糖醇或L-阿托糖醇相同结构的脱氧多羟基化脱氢酶的能力。 肠杆菌属细菌细胞IK7（登录号NITE P-271）。 一种生产脱氧酮糖酮的方法，包括使含有通过培养本发明的微生物获得的脱氧多元醇脱氢酶的培养物或使脱氧多元醇脱氢酶与位置C2和C3处于相同结构的含有脱氧多羟基化合物的溶液反应，作为 核糖醇或L-天冬氨酸氧化脱氧多元醇以产生相应的脱氧酮糖，然后收集脱氧酮糖酮。 脱氧多元醇是1-脱氧-D-多糖，而相应的脱氧酮基是1-脱氧-L-精氨酸。 否则，脱氧多元醇为L-麦芽糖醇，而相应的脱氧酮糖为1-脱氧-L-果糖。

公开(公告)号：US08389248B2

公开(公告)日：2013-03-05

申请号：US12515605

申请日：2007-11-20

申请人： Ken Izumori ,  Masaaki Tokuda ,  George Fleet ,  Yoshio Tsujisaka ,  Kei Takeshita ,  Keiji Tsusaki ,  Kazuhiro Okuma

发明人： Ken Izumori ,  Masaaki Tokuda ,  George Fleet ,  Yoshio Tsujisaka ,  Kei Takeshita ,  Keiji Tsusaki ,  Kazuhiro Okuma

IPC分类号： C12P19/24 ,  C12N9/90

CPC分类号： C07H3/02 ,  C12N9/92 ,  C12P19/24

摘要： Providing 1- or 6-deoxy products corresponding to all of aldohexoses, ketohexoses and sugar alcohols, as based on Deoxy-Izumoring, as well as a method for systematically producing those products. A method for producing deoxyketohexose and a derivative thereof using a deoxyketohexose isomerase derived from Pseudomonas cichorii ST-24 (FERM BP-2736), comprising epimerizing 1-deoxy D-ketohexose or 6-deoxy D-ketohexose or 1-deoxy L-ketohexose or 6-deoxy L-ketohexose at position 3 to produce the individually corresponding 1-deoxy D-ketohexose or 6-deoxy D-ketohexose or 1-deoxy L-ketohexose or 6-deoxy L-ketohexose as an intended product.

摘要翻译： 提供对应于所有醛脱水己酮糖，酮己立糖和糖醇的1-脱氧产物或6-脱氧产物，以脱氧消肿为基础，以及一种系统地生产这些产品的方法。 使用衍生自奇异假单胞菌ST-24（FERM BP-2736）的脱氧己糖异构酶生产脱氧己糖及其衍生物的方法，其包括将1-脱氧D-酮己糖或6-脱氧D-酮己糖或1-脱氧L-酮己糖或 6-脱氧L-酮基己糖，以产生单独相应的1-脱氧D-酮己糖或6-脱氧D-酮己糖或1-脱氧L-酮己糖或6-脱氧L-酮己糖作为目标产物。

公开(公告)号：US07691619B2

公开(公告)日：2010-04-06

申请号：US11660959

申请日：2005-08-23

申请人： Ken Izumori ,  Goro Takata ,  Masaaki Tokuda

发明人： Ken Izumori ,  Goro Takata ,  Masaaki Tokuda

IPC分类号： C12N9/90 ,  C12P19/24 ,  C12P19/02 ,  C12P21/04 ,  C07H21/04

CPC分类号： C12P19/24 ,  C12N9/90

摘要： The invention relates to an isolated protein including an amino acid sequence represented by SEQ ID NO:2 and having an L-rhamnose isomerase activity. This novel enzyme has a higher reaction efficiency between D-psicose and D-allose and is excellent in thermal stability.

摘要翻译： 本发明涉及包含SEQ ID NO：2所示的氨基酸序列并具有L-鼠李糖异构酶活性的分离蛋白质。 该新型酶具有较高的D-木糖和D-丙氨酸的反应效率，热稳定性优异。

公开(公告)号：US09668958B2

公开(公告)日：2017-06-06

申请号：US13369780

申请日：2012-02-09

申请人： Tetsuo Iida ,  Takashi Ichihara ,  Ken Izumori ,  Masaaki Tokuda ,  Takaaki Ogawa

发明人： Tetsuo Iida ,  Takashi Ichihara ,  Ken Izumori ,  Masaaki Tokuda ,  Takaaki Ogawa

IPC分类号： A61K8/60 ,  A61Q11/00 ,  A61K31/047 ,  A61K31/7004 ,  C07H3/02

CPC分类号： A61K8/60 ,  A61K31/047 ,  A61K31/7004 ,  A61Q11/00 ,  C07H3/02

摘要： To provide a composition for preventing periodontal diseases (prophylactic agent of periodontal diseases), the composition having an excellent cariostatic property, being safe and stable for prolonged use and having less effects on flavor. A non-cariogenic material prepared by blending a rare sugar in the D form as selected from the group consisting of D-psicose, D-sorbose and D-tagatose, a rare sugar in the L form as selected from L-fructose, L-psicose and L-tagatose, or allitol as a rare sugar derivative, singly or in combination. A cariostatic agent comprising D-psicose, D-sorbose, D-tagatose, L-fructose, L-psicose and/or L-tagatose. A cariostatic agent in combination with catechins.