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23 条记录 (耗时 0.038 秒)

    Methods and materials for expression of a recombinant protein
    1.
    发明授权
    Methods and materials for expression of a recombinant protein 有权
    用于表达重组蛋白的方法和材料

    公开(公告)号：US07794976B2

    公开(公告)日：2010-09-14

    申请号：US11831691

    申请日：2007-07-31

    申请人： Masahisa Handa ,  Arnold H. Horwitz ,  Robyn Cotter ,  Eddie Batista

    发明人： Masahisa Handa ,  Arnold H. Horwitz ,  Robyn Cotter ,  Eddie Batista

    IPC分类号： C12P21/04 ,  C12N15/00 ,  C12N5/12

    CPC分类号： C07K16/00 ,  C12N7/00 ,  C12N15/85 ,  C12N2710/16221 ,  C12N2710/16243 ,  C12N2800/108 ,  C12N2820/10 ,  C12N2820/55 ,  C12N2820/60 ,  C12N2830/42

    摘要： Recombinant expression vectors are provided comprising a 3′UTR of a light chain and an Epstein-Barr virus origin of replication. Also provided are host cells comprising such vectors and methods of producing recombinant protein with such vectors. Additional methods of producing a recombinant protein involve contacting cells with a first and second vector, each of which encode a different polypeptide chain, and wherein the second vector is present in an amount which is about 1.5 to 2.5 times as much as that of the first vector. Cells also can be transfected with a recombinant transient expression vector encoding a protein and are cultured in a medium in a membrane-enhanced culturing vessel to produce recombinant protein.

    摘要翻译： 提供重组表达载体，其包含轻链的3'UTR和Epstein-Barr病毒复制起点。 还提供了包含这种载体的宿主细胞和用这种载体产生重组蛋白的方法。 生产重组蛋白质的其它方法包括使细胞与第一和第二载体接触，第一和第二载体各自编码不同的多肽链，其中第二载体的存在量为第一载体的约1.5至2.5倍 向量。 细胞也可以用编码蛋白质的重组瞬时表达载体转染，并在膜增强培养容器中的培养基中培养以产生重组蛋白。

    Method of treating an IL-1 related cancer
    5.
    发明授权
    Method of treating an IL-1 related cancer 有权
    治疗IL-1相关癌症的方法

    公开(公告)号：US07829094B2

    公开(公告)日：2010-11-09

    申请号：US12464061

    申请日：2009-05-11

    申请人： Linda Masat ,  Mary Haak-Frendscho ,  Arnold H. Horwitz ,  Gang Chen ,  Marina Roell

    发明人： Linda Masat ,  Mary Haak-Frendscho ,  Arnold H. Horwitz ,  Gang Chen ,  Marina Roell

    IPC分类号： A61K39/395

    CPC分类号： C07K16/244 ,  A61K2039/505 ,  C07K16/245 ,  C07K2317/21 ,  C07K2317/34 ,  C07K2317/76 ,  C07K2317/92 ,  Y10S514/885

    摘要： Methods of treating or preventing an IL-1 related disease or condition in a mammal comprising administering an effective amount of an IL-1β binding antibody or IL-1β binding fragment thereof. An IL-1β binding antibody or IL-1β binding fragment thereof is provided comprising the amino acid sequence of SEQ ID NO: 15 and SEQ ID NO: 11, and related nucleic acids, vectors, cells, and compositions, and a method of preparing an affinity matured IL-1β binding polypeptide. IL-1β binding antibodies or IL-1β binding fragments thereof are provided which have desirable affinity and potency.

    摘要翻译： 治疗或预防哺乳动物中IL-1相关疾病或病症的方法，包括给予有效量的IL-1和bgr; 结合抗体或IL-1和bgr; 其结合片段。 IL-1和bgr 结合抗体或IL-1和bgr; 提供了包含SEQ ID NO：15和SEQ ID NO：11的氨基酸序列以及相关核酸，载体，细胞和组合物的结合片段，以及制备亲和力成熟的IL-1和bgr的方法。 结合多肽。 IL-1和bgr 结合抗体或IL-1和bgr; 提供其结合片段，其具有期望的亲和力和效力。

    Methods and materials for increasing expression of recombinant polypeptides
    6.
    发明授权
    Methods and materials for increasing expression of recombinant polypeptides 有权

    公开(公告)号：US07192737B2

    公开(公告)日：2007-03-20

    申请号：US10404724

    申请日：2003-03-31

    申请人： Arnold H. Horwitz

    发明人： Arnold H. Horwitz

    IPC分类号： C12P21/02

    CPC分类号： C12N15/85 ,  C12N15/63 ,  C12N15/65 ,  C12N15/66 ,  C12N2800/107 ,  C12N2830/00 ,  C12P21/02

    摘要： The present invention provides novel methods and materials for increasing the expression of recombinant polypeptides. Methods and materials of the invention allow increased expression of transcription units that include recombinant DNA sequences which encode polypeptides of interest. The present invention provides expression vectors which contain multiple copies of a transcription unit encoding a polypeptide of interest separated by at least one selective marker gene and methods for sequentially transforming or transfecting host cells with expression vectors to increase transcription unit dosage and expression.

    Modular assembly of antibody genes, antibodies prepared thereby and use
    7.
    发明授权
    Modular assembly of antibody genes, antibodies prepared thereby and use 失效
    抗体基因的模块化装配，由此制备的抗体和使用

    公开(公告)号：US06204023B1

    公开(公告)日：2001-03-20

    申请号：US08472691

    申请日：1995-06-06

    申请人： Randy R. Robinson ,  Alvin Y. Liu ,  Arnold H. Horwitz ,  Marc Better ,  Randolph Wall ,  Shau-Ping Lei ,  Gary L. Wilcox

    发明人： Randy R. Robinson ,  Alvin Y. Liu ,  Arnold H. Horwitz ,  Marc Better ,  Randolph Wall ,  Shau-Ping Lei ,  Gary L. Wilcox

    IPC分类号： A61K39395

    CPC分类号： C12N15/70 ,  A61K38/00 ,  C07K14/43 ,  C07K16/3046 ,  C07K16/462 ,  C07K2317/24 ,  C07K2317/732 ,  C07K2317/734 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/034 ,  C07K2319/036 ,  C07K2319/30 ,  C12N9/88 ,  C12N15/81

    摘要： The invention relates to cDNA genetic sequences, vehicles containing same as well as hosts tansformed therewith, for the production of chimeric immunoglobulin molecules, functional fragments thereof and immunoglobulin derivatives exhibiting novel functional properties comprising human constant region modules and non-human variable region modules, or for class switching antibody molecules and/or chains. The invention also relates to DNA coding for pectate lyase signal peptide has been cloned on a plasmid to create a secretion vector which is capable of producing a chosen protein which is transported across the bacterial membrane. The secretion vector has been used to secrete extracellular thaumatin and extracellular chimeric antibody fragments. The proteins produced by this vector have biological activity. The thaumatin is properly folded and the antibody fragments are capable of binding antigens on target cancer cells. The invention also relates to the secretion of chimeric antibodies and fragments thereof from yeast in functional form.

    摘要翻译： 本发明涉及cDNA遗传序列，含有其的载体以及与其一起改造的宿主，用于产生嵌合免疫球蛋白分子，其功能片段和表现出包括人恒定区模块和非人可变区模块的新功能特性的免疫球蛋白衍生物，或 用于类交换抗体分子和/或链。本发明还涉及将编码果胶酶裂解酶信号肽的DNA克隆在质粒上以产生能够产生选择的蛋白质的分泌载体，所述蛋白质通过细菌膜传输。 已经使用分泌载体来分泌细胞外的奇异果甜蛋白和细胞外嵌合抗体片段。 由该载体产生的蛋白质具有生物活性。 正确地折叠奇异果甜蛋白，抗体片段能够结合靶癌细胞上的抗原。 本发明还涉及从功能形式的酵母中分泌嵌合抗体及其片段。

    Antibodies to high molecular weight melanoma associated antigen
    8.
    发明授权
    Antibodies to high molecular weight melanoma associated antigen 有权
    高分子量黑素瘤相关抗原的抗体

    公开(公告)号：US08318162B2

    公开(公告)日：2012-11-27

    申请号：US13384197

    申请日：2010-07-16

    申请人： Arnold H. Horwitz

    发明人： Arnold H. Horwitz

    IPC分类号： A61K39/395 ,  A61K39/00 ,  G01N33/53 ,  C07K16/28 ,  C12P21/08

    CPC分类号： C07K16/3053 ,  C07K2317/24

    摘要： The present disclosure relates to anti-high molecular weight melanoma associated antigen (HMW-MAA) antibodies which bind to human HMW-MAA. Such antibodies may be used to treat diseases or disorders characterized by expression of HMW-MAA including cancer, for example, melanoma, basal cell carcinoma, ALL or AML.

    摘要翻译： 本公开涉及结合人HMW-MAA的抗高分子量黑素瘤相关抗原（HMW-MAA）抗体。 这样的抗体可用于治疗特征在于包括癌症的HMW-MAA表达的疾病或病症，例如黑素瘤，基底细胞癌，ALL或AML。

    METHODS AND MATERIALS FOR INCREASING EXPRESSION OF RECOMBINANT POLYPEPTIDES
    9.
    发明申请
    METHODS AND MATERIALS FOR INCREASING EXPRESSION OF RECOMBINANT POLYPEPTIDES 有权
    增加重组多肽表达的方法和材料

    公开(公告)号：US20120178126A1

    公开(公告)日：2012-07-12

    申请号：US13205448

    申请日：2011-08-08

    申请人： Arnold H. Horwitz

    发明人： Arnold H. Horwitz

    IPC分类号： C12P21/00 ,  C12N5/10 ,  C12N15/85

    CPC分类号： C12N15/85 ,  C12N15/63 ,  C12N15/65 ,  C12N15/66 ,  C12N2800/107 ,  C12N2830/00 ,  C12P21/02

    摘要： The present invention provides novel methods and materials for increasing the expression of recombinant polypeptides. Methods and materials of the invention allow increased expression of transcription units that include recombinant DNA sequences which encode polypeptides of interest. The present invention provides expression vectors which contain multiple copies of a transcription unit encoding a polypeptide of interest separated by at least one selective marker gene and methods for sequentially transforming or transfecting host cells with expression vectors to increase transcription unit dosage and expression.

    摘要翻译： 本发明提供了用于增加重组多肽表达的新方法和材料。 本发明的方法和材料允许增加包括编码感兴趣多肽的重组DNA序列的转录单位的表达。 本发明提供表达载体，其含有编码由至少一种选择标记基因分离的感兴趣多肽的转录单位的多个拷贝，以及用表达载体依次转化或转染宿主细胞以增加转录单位剂量和表达的方法。