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    Methods and materials for expression of a recombinant protein
    1.
    发明授权
    Methods and materials for expression of a recombinant protein 有权
    用于表达重组蛋白的方法和材料

    公开(公告)号：US07794976B2

    公开(公告)日：2010-09-14

    申请号：US11831691

    申请日：2007-07-31

    申请人： Masahisa Handa ,  Arnold H. Horwitz ,  Robyn Cotter ,  Eddie Batista

    发明人： Masahisa Handa ,  Arnold H. Horwitz ,  Robyn Cotter ,  Eddie Batista

    IPC分类号： C12P21/04 ,  C12N15/00 ,  C12N5/12

    CPC分类号： C07K16/00 ,  C12N7/00 ,  C12N15/85 ,  C12N2710/16221 ,  C12N2710/16243 ,  C12N2800/108 ,  C12N2820/10 ,  C12N2820/55 ,  C12N2820/60 ,  C12N2830/42

    摘要： Recombinant expression vectors are provided comprising a 3′UTR of a light chain and an Epstein-Barr virus origin of replication. Also provided are host cells comprising such vectors and methods of producing recombinant protein with such vectors. Additional methods of producing a recombinant protein involve contacting cells with a first and second vector, each of which encode a different polypeptide chain, and wherein the second vector is present in an amount which is about 1.5 to 2.5 times as much as that of the first vector. Cells also can be transfected with a recombinant transient expression vector encoding a protein and are cultured in a medium in a membrane-enhanced culturing vessel to produce recombinant protein.

    摘要翻译： 提供重组表达载体，其包含轻链的3'UTR和Epstein-Barr病毒复制起点。 还提供了包含这种载体的宿主细胞和用这种载体产生重组蛋白的方法。 生产重组蛋白质的其它方法包括使细胞与第一和第二载体接触，第一和第二载体各自编码不同的多肽链，其中第二载体的存在量为第一载体的约1.5至2.5倍 向量。 细胞也可以用编码蛋白质的重组瞬时表达载体转染，并在膜增强培养容器中的培养基中培养以产生重组蛋白。

    Methods and Materials for Expression of a Recombinant Protein
    2.
    发明申请
    Methods and Materials for Expression of a Recombinant Protein 有权
    用于表达重组蛋白的方法和材料

    公开(公告)号：US20080145894A1

    公开(公告)日：2008-06-19

    申请号：US11831691

    申请日：2007-07-31

    申请人： Masahisa Handa ,  Arnold Horwitz ,  Eddie Bautista ,  Robyn Cotter

    发明人： Masahisa Handa ,  Arnold Horwitz ,  Eddie Bautista ,  Robyn Cotter

    IPC分类号： C12P21/04

    CPC分类号： C07K16/00 ,  C12N7/00 ,  C12N15/85 ,  C12N2710/16221 ,  C12N2710/16243 ,  C12N2800/108 ,  C12N2820/10 ,  C12N2820/55 ,  C12N2820/60 ,  C12N2830/42

    摘要： Recombinant expression vectors are provided comprising a 3′UTR of a light chain and an Epstein-Barr virus origin of replication. Also provided are host cells comprising such vectors and methods of producing recombinant protein with such vectors. Additional methods of producing a recombinant protein involve contacting cells with a first and second vector, each of which encode a different polypeptide chain, and wherein the second vector is present in an amount which is about 1.5 to 2.5 times as much as that of the first vector. Cells also can be transfected with a recombinant transient expression vector encoding a protein and are cultured in a medium in a membrane-enhanced culturing vessel to produce recombinant protein.

    摘要翻译： 提供重组表达载体，其包含轻链的3'UTR和Epstein-Barr病毒复制起点。 还提供了包含这种载体的宿主细胞和用这种载体产生重组蛋白的方法。 生产重组蛋白质的其它方法包括使细胞与第一和第二载体接触，第一和第二载体各自编码不同的多肽链，其中第二载体的存在量为第一载体的约1.5至2.5倍 向量。 细胞也可以用编码蛋白质的重组瞬时表达载体转染，并在膜增强培养容器中的培养基中培养以产生重组蛋白。

    Methods and materials for expression of a recombinant protein
    3.
    发明申请
    Methods and materials for expression of a recombinant protein 审中-公开
    用于表达重组蛋白的方法和材料

    公开(公告)号：US20060121604A1

    公开(公告)日：2006-06-08

    申请号：US11295006

    申请日：2005-12-05

    申请人： Masahisa Handa ,  Arnold Horwitz ,  Eddie Bautista ,  Robyn Cotter

    发明人： Masahisa Handa ,  Arnold Horwitz ,  Eddie Bautista ,  Robyn Cotter

    IPC分类号： C12Q1/68 ,  C12N15/63 ,  C12N15/74 ,  C12N15/00 ,  C12N15/70 ,  C12N15/09

    CPC分类号： C07K16/00 ,  C12N7/00 ,  C12N15/85 ,  C12N2710/16221 ,  C12N2710/16243 ,  C12N2800/108 ,  C12N2820/10 ,  C12N2820/55 ,  C12N2820/60 ,  C12N2830/42

    摘要： Recombinant expression vectors are provided comprising a 3′ UTR of a light chain and an Epstein-Barr virus origin of replication. Also provided are host cells comprising such vectors and methods of producing recombinant protein with such vectors. Additional methods of producing a recombinant protein involve contacting cells with a first and second vector, each of which encode a different polypeptide chain, and wherein the second vector is present in an amount which is about 1.5 to 2.5 times as much as that of the first vector. Cells also can be transfected with a recombinant transient expression vector encoding a protein and are cultured in a medium in a membrane-enhanced culturing vessel to produce recombinant protein.

    摘要翻译： 提供重组表达载体，其包含轻链的3'UTR和Epstein-Barr病毒复制起点。 还提供了包含这种载体的宿主细胞和用这种载体产生重组蛋白的方法。 生产重组蛋白质的其它方法包括使细胞与第一和第二载体接触，第一和第二载体各自编码不同的多肽链，其中第二载体的存在量为第一载体的约1.5至2.5倍 向量。 细胞也可以用编码蛋白质的重组瞬时表达载体转染，并在膜增强培养容器中的培养基中培养以产生重组蛋白。

    Methods and Materials for Expression of a Recombinant Protein
    4.
    发明申请
    Methods and Materials for Expression of a Recombinant Protein 审中-公开
    用于表达重组蛋白的方法和材料

    公开(公告)号：US20080124762A1

    公开(公告)日：2008-05-29

    申请号：US11831882

    申请日：2007-07-31

    申请人： Masahisa Handa ,  Arnold Horwitz ,  Eddie Bautista ,  Robyn Cotter

    发明人： Masahisa Handa ,  Arnold Horwitz ,  Eddie Bautista ,  Robyn Cotter

    IPC分类号： C12P21/04 ,  C12N15/00 ,  C12N5/06

    CPC分类号： C07K16/00 ,  C12N7/00 ,  C12N15/85 ,  C12N2710/16221 ,  C12N2710/16243 ,  C12N2800/108 ,  C12N2820/10 ,  C12N2820/55 ,  C12N2820/60 ,  C12N2830/42

    摘要： Recombinant expression vectors are provided comprising a 3′ UTR of a light chain and an Epstein-Barr virus origin of replication. Also provided are host cells comprising such vectors and methods of producing recombinant protein with such vectors. Additional methods of producing a recombinant protein involve contacting cells with a first and second vector, each of which encode a different polypeptide chain, and wherein the second vector is present in an amount which is about 1.5 to 2.5 times as much as that of the first vector. Cells also can be transfected with a recombinant transient expression vector encoding a protein and are cultured in a medium in a membrane-enhanced culturing vessel to produce recombinant protein.

    摘要翻译： 提供重组表达载体，其包含轻链的3'UTR和Epstein-Barr病毒复制起点。 还提供了包含这种载体的宿主细胞和用这种载体产生重组蛋白的方法。 生产重组蛋白质的其它方法包括使细胞与第一和第二载体接触，第一和第二载体各自编码不同的多肽链，其中第二载体的存在量为第一载体的约1.5至2.5倍 向量。 细胞也可以用编码蛋白质的重组瞬时表达载体转染，并在膜增强培养容器中的培养基中培养以产生重组蛋白。