公开(公告)号：US06251247B1

公开(公告)日：2001-06-26

申请号：US09281385

申请日：1999-03-30

申请人： Masato Mitsuhashi ,  Mieko Ogura ,  Kenji Watanabe ,  Yoichi Agata

发明人： Masato Mitsuhashi ,  Mieko Ogura ,  Kenji Watanabe ,  Yoichi Agata

IPC分类号： G01N2726

CPC分类号： G01N27/44726

摘要： Degradation of RNA present in a sample is detected by using, as an indicator, rRNA included in the RNA. The steps include: (a) introducing the sample onto a microchannel, at an introducing point, filled with an electrophoresis separation gel; (b) conducting electrophoresis of rRNA fragments present in the sample to force rRNA fragments to migrate through the electrophoresis separation gel; (c) detecting rRNA fragments passing through a detection point located downstream of the introducing point, to obtain detection patterns; and (d) determining degradation of RNA present in the sample based on the detection patterns of rRNA. A sample containing degraded mRNA can also be screened by detecting rRNA degradation in the above microchannel electrophoresis.

摘要翻译： 通过使用RNA中包含的rRNA作为指标来检测样品中存在的RNA的降解。 步骤包括：（a）将样品引入微通道，在引入点处填充有电泳分离凝胶; （b）进行样品中存在的rRNA片段的电泳以迫使rRNA片段迁移通过电泳分离凝胶; （c）检测通过位于引入点下游的检测点的rRNA片段，以获得检测图案; 和（d）基于rRNA的检测模式确定样品中存在的RNA的降解。 还可以通过检测上述微通道电泳中的rRNA降解来筛选含有降解的mRNA的样品。

公开(公告)号：US5545528A

公开(公告)日：1996-08-13

申请号：US379077

申请日：1995-01-26

申请人： Masato Mitsuhashi ,  Mieko Ogura

发明人： Masato Mitsuhashi ,  Mieko Ogura

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6816

摘要： The present invention relates to a method for the rapid screening of gene amplification products on commercially available polypropylene microtiter plates. In one aspect of the invention, polypropylene microtiter plates are used for polymerase chain reaction (PCR) and the amount of nucleic acid sequences amplified through the reaction is quantitated in the same plates. In another aspect of the invention, polypropylene plates are used for detection and quantification of nucleic acids.

摘要翻译： 本发明涉及在市售的聚丙烯微量滴定板上快速筛选基因扩增产物的方法。 在本发明的一个方面，聚丙烯微量滴定板用于聚合酶链式反应（PCR），通过反应扩增的核酸序列的量在相同的平板中定量。 在本发明的另一方面，聚丙烯板用于检测和定量核酸。

公开(公告)号：US20090263837A1

公开(公告)日：2009-10-22

申请号：US11913052

申请日：2006-04-28

申请人： Masato Mitsuhashi ,  Mieko Ogura

发明人： Masato Mitsuhashi ,  Mieko Ogura

IPC分类号： G01N33/53 ,  G01N33/543 ,  C12P19/34

CPC分类号： B01L3/50853 ,  B01L2300/046 ,  B01L2300/0654 ,  C12Q1/6806 ,  Y10S436/809 ,  C12Q2527/137

摘要： A method is provided for performing a reaction, such as the synthesis of concentrated cDNA, in the wells of a microplate while minimizing the volume of the solution of reagents required to perform the reaction. In the method, a pestle is inserted into the well of a microplate to which a substance has been immobilized. A volume of reagent solution is introduced into the well that is insufficient to cover the portion of the well onto which the substance is immobilized. The insertion of the pestle displaces reagent solution and increases the surface area of the solution in contact with the portion of the well to which the substance has been immobilized when the pestle is inserted.

摘要翻译： 提供了一种方法，用于在微量培养板的孔中进行诸如合成浓缩cDNA的反应，同时使进行反应所需的试剂溶液的体积最小化。 在该方法中，将杵插入已经固定有物质的微孔板的孔中。 将一定体积的试剂溶液引入孔中，其不足以覆盖固定有物质的孔的部分。 当插入杵时，杵的插入取代试剂溶液并增加溶液的表面积，该表面积与物质固定的孔的部分接触。

公开(公告)号：US5554502A

公开(公告)日：1996-09-10

申请号：US147936

申请日：1993-11-05

申请人： Masato Mitsuhashi ,  Mieko Ogura

发明人： Masato Mitsuhashi ,  Mieko Ogura

IPC分类号： C12M1/34 ,  C12Q1/44 ,  C12Q1/00 ,  C12N15/00 ,  C12Q1/68 ,  G01N21/76

CPC分类号： C12Q1/44 ,  G01N2333/922 ,  Y10S436/80

摘要： The present invention relates to analyzing a test sample for the presence or absence of nuclease activity, and for quantifying the amount of nuclease activity. In one aspect of the invention, a kit is provided which allows the rapid and efficient quantification of nuclease activity in a sample having an unknown nuclease activity. In another aspect of the invention, a method for determining the presence or absence of nuclease activity, and for quantifying nuclease activity is disclosed.

摘要翻译： 本发明涉及分析测试样品中核酸酶活性的存在或不存在以及用于量化核酸酶活性的量。 在本发明的一个方面，提供了一种试剂盒，其允许在具有未知核酸酶活性的样品中快速且有效地定量核酸酶活性。 在本发明的另一方面，公开了确定核酸酶活性的存在或不存在以及用于定量核酸酶活性的方法。

公开(公告)号：US07816081B2

公开(公告)日：2010-10-19

申请号：US11913052

申请日：2006-04-28

申请人： Masato Mitsuhashi ,  Mieko Ogura

发明人： Masato Mitsuhashi ,  Mieko Ogura

IPC分类号： C12Q1/68

CPC分类号： B01L3/50853 ,  B01L2300/046 ,  B01L2300/0654 ,  C12Q1/6806 ,  Y10S436/809 ,  C12Q2527/137

摘要： A method is provided for performing a reaction, such as the synthesis of concentrated cDNA, in the wells of a microplate while minimizing the volume of the solution of reagents required to perform the reaction. In the method, a pestle is inserted into the well of a microplate to which a substance has been immobilized. A volume of reagent solution is introduced into the well that is insufficient to cover the portion of the well onto which the substance is immobilized. The insertion of the pestle displaces reagent solution and increases the surface area of the solution in contact with the portion of the well to which the substance has been immobilized when the pestle is inserted.

摘要翻译： 提供了一种方法，用于在微量培养板的孔中进行诸如合成浓缩cDNA的反应，同时使进行反应所需的试剂溶液的体积最小化。 在该方法中，将杵插入已经固定有物质的微孔板的孔中。 将一定体积的试剂溶液引入孔中，其不足以覆盖固定有物质的孔的部分。 当插入杵时，杵的插入取代试剂溶液并增加溶液的表面积，该表面积与物质固定的孔的部分接触。

公开(公告)号：US06638428B2

公开(公告)日：2003-10-28

申请号：US10003176

申请日：2001-10-31

申请人： Mieko Ogura

发明人： Mieko Ogura

IPC分类号： B01D6100

CPC分类号： G01N27/44704 ,  B01L3/5025 ,  B01L2300/0618 ,  B01L2300/0829 ,  B01L2400/0421 ,  G01N27/44756 ,  G01N2001/4016 ,  G01N2001/4038

摘要： Formation of bubbles is prevented during filtration operation by placing an oil on top of a solution-to-be filtered, where the oil is not admixable with the solution and has a lower specific gravity than the solution. The oil seals the solution from air so that no bubbles are formed. The oil does not pass through the filter even after all collectable filtrate of the solution has passed therethrough.