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    Method of producing lens cell and lens cell obtained by the method
    1.
    发明申请
    Method of producing lens cell and lens cell obtained by the method 审中-公开
    通过该方法获得的透镜细胞和透镜细胞的制造方法

    公开(公告)号：US20070037282A1

    公开(公告)日：2007-02-15

    申请号：US10551378

    申请日：2004-03-22

    申请人： Masayo Takahashi ,  Yoshiki Sasai ,  Hiroshi Kawasaki ,  Sotaro Ooto

    发明人： Masayo Takahashi ,  Yoshiki Sasai ,  Hiroshi Kawasaki ,  Sotaro Ooto

    IPC分类号： C12N5/08

    CPC分类号： C12N5/0621 ,  C12N2501/115 ,  C12N2506/02

    摘要： A method of producing a lens cell is disclosed. The method includes: an ES cell maintenance step of maintaining an ES cell by using a medium containing a fibroblast growth factor FGF-2 at a concentration of 2 ng/ml to 50 ng/ml; and a differentiation inducing step, carried out after the ES cell maintenance step, of inducing differentiation of the ES cell into a lens cell by implanting and culturing the ES cell on a mouse-skull-cell PA6 at a cell density of 2 colonies/cm2 to 6.5 colonies/cm2. In one embodiment, a washing step, carried out between the ES cell maintenance step and the differentiation inducing step, of washing the maintained ES cell once with an ES differentiation medium is included.

    摘要翻译： 公开了一种制造透镜单元的方法。 该方法包括：通过使用浓度为2ng / ml至50ng / ml的含有成纤维细胞生长因子FGF-2的培养基来维持ES细胞的ES细胞维持步骤; 以及在ES细胞维持步骤之后进行的分化诱导步骤，通过以2个菌落/ cm 2的细胞密度将ES细胞植入和培养到小鼠颅骨细胞PA6上来诱导ES细胞分化成晶状体细胞 2至6.5个菌落/ cm 2。 在一个实施方案中，包括在ES细胞维持步骤和分化诱导步骤之间进行的洗涤步骤，用ES分化培养基洗涤维持的ES细胞一次。

    Method of inducing the differentiation of embryonic stem cells into nerve by serum-free suspension culture
    3.
    发明授权
    Method of inducing the differentiation of embryonic stem cells into nerve by serum-free suspension culture 有权
    通过无血清悬浮培养诱导胚胎干细胞分化为神经的方法

    公开(公告)号：US08492147B2

    公开(公告)日：2013-07-23

    申请号：US11570579

    申请日：2005-06-16

    申请人： Yoshiki Sasai ,  Kiichi Watanabe

    发明人： Yoshiki Sasai ,  Kiichi Watanabe

    IPC分类号： C12N5/00 ,  C12N5/02

    CPC分类号： C12N5/0619 ,  C12N5/062 ,  C12N5/0621 ,  C12N2506/02

    摘要： The present invention provides a clinically applicable method of inducing differentiation of embryonic stem cells, particularly a method of inducing differentiation of embryonic stem cells into forebrain neurons. More specifically, the present invention provides a method of inducing differentiation of embryonic stem cells, comprising culturing the embryonic stem cells as a floating aggregate in a serum-free medium, particularly a method of inducing differentiation of the embryonic stem cells into nervous system cells such as forebrain neurons and cerebellar neurons and sensory organ cells; a floating aggregate of embryonic stem cells obtained by culturing the embryonic stem cells as a floating aggregate in a serum-free medium; and cells derived from a floating aggregate of embryonic stem cells, particularly nervous system cells such as forebrain neurons and cerebellar neuron, sensory organ cells such as retinal precursor cells, and the like.

    摘要翻译： 本发明提供了诱导胚胎干细胞分化的临床适用方法，特别是诱导胚胎干细胞分化为前脑神经元的方法。 更具体地，本发明提供了诱导胚胎干细胞分化的方法，包括将胚胎干细胞作为浮动聚集体培养在无血清培养基中，特别是诱导将胚胎干细胞分化为神经系统细胞的方法 作为前脑神经元和小脑神经元和感觉器官细胞; 通过将胚胎干细胞作为浮动聚集体培养在无血清培养基中而获得的胚胎干细胞的浮动聚集体; 以及衍生自胚胎干细胞，特别是神经系统细胞如前脑神经元和小脑神经元，感觉器官细胞如视网膜前体细胞等的浮动聚集体的细胞。

    Method of culturing embryonic stem cells with the use of amniotic membrane-origin factor
    4.
    发明授权
    Method of culturing embryonic stem cells with the use of amniotic membrane-origin factor 失效
    使用羊膜来源因子培养胚胎干细胞的方法

    公开(公告)号：US07923246B2

    公开(公告)日：2011-04-12

    申请号：US11569414

    申请日：2005-05-27

    申请人： Yoshiki Sasai ,  Morio Ueno ,  Shigeru Kinoshita

    发明人： Yoshiki Sasai ,  Morio Ueno ,  Shigeru Kinoshita

    IPC分类号： C12N5/00 ,  C12N5/02 ,  C12N5/071

    CPC分类号： G01N33/5073 ,  C12N5/0606 ,  C12N5/0619 ,  C12N2506/02 ,  G01N33/5008 ,  G01N33/502 ,  G01N33/5058

    摘要： The present invention provides a clinically applicable method of inducing differentiation of the embryonic stem cells. Specifically, the present invention provides a method of culturing embryonic stem cells, which comprises culturing embryonic stem cells in the presence of an amnion-derived factor, a cell culture obtained by the culture method, and a culture agent/culture kit of embryonic stem cells comprising an amnion-derived factor. The present invention also provides a method of screening an amnion-derived factor having an activity useful for culturing embryonic stem cells with the activity as an index, and an amniocyte-derived factor obtained by the screening method. Furthermore, the present invention provides a method of preparing amniocytes useful for culturing embryonic stem cells, amniocytes obtained by the preparation method, and the like.

    摘要翻译： 本发明提供诱导胚胎干细胞分化的临床适用方法。 具体地，本发明提供培养胚胎干细胞的方法，其包括在羊膜来源的因子存在下培养胚胎干细胞，通过培养方法获得的细胞培养物和胚胎干细胞的培养试剂/培养试剂盒 包括羊膜衍生因子。 本发明还提供一种筛选具有活性作为指标培养胚胎干细胞的活性的羊膜衍生因子和通过筛选方法得到的羊膜细胞衍生因子的方法。 此外，本发明提供了制备用于培养胚胎干细胞的羊膜细胞的方法，通过制备方法获得的羊膜细胞等。

    Method of Producing Nerve Cell
    6.
    发明申请
    Method of Producing Nerve Cell 审中-公开
    产生神经细胞的方法

    公开(公告)号：US20080305544A1

    公开(公告)日：2008-12-11

    申请号：US11571580

    申请日：2005-07-06

    申请人： Hiroo Iwata ,  Yoshiki Sasai ,  Hironori Yamazoe ,  Masato Kobori ,  Mitsuo Satoh ,  Keiichi Yano

    发明人： Hiroo Iwata ,  Yoshiki Sasai ,  Hironori Yamazoe ,  Masato Kobori ,  Mitsuo Satoh ,  Keiichi Yano

    IPC分类号： C12N5/06

    CPC分类号： C12N5/0619 ,  C12N2500/38 ,  C12N2506/02

    摘要： An Object of the present invention is to provide a process for producing a nerve cell by inducing differentiation of an embryonic stem cell, a method for inducing differentiation of the embryonic stem cell into a nerve cell, a medium to be used in the production process or differentiation induction method, or a method for improving purity of the nerve cell obtained by inducing differentiation of the embryonic stem cell. The present invention provides a process for producing a nerve cell which is applicable to treatment of neurodegenerative disease or the like easily, selectively or inexpensively by inducing differentiation induction of an embryonic stem cell using vitamin B12 or a salt thereof and heparin, a substance having heparin-like activity or a salt.

    摘要翻译： 本发明的目的是提供一种通过诱导胚胎干细胞的分化来产生神经细胞的方法，用于诱导将胚胎干细胞分化成神经细胞的方法，用于生产过程的培养基或 分化诱导方法或通过诱导胚胎干细胞分化而获得的提高神经细胞的纯度的方法。 本发明提供了通过使用维生素B12或其盐诱导胚胎干细胞的分化诱导和肝素（含有肝素的物质），可以容易地，选择性地或廉价地治疗神经变性疾病等的神经细胞的制造方法 类活动或盐。

    METHOD FOR DIFFERENTIATION INDUCTION IN CULTURED STEM CELLS
    7.
    发明申请
    METHOD FOR DIFFERENTIATION INDUCTION IN CULTURED STEM CELLS 审中-公开
    培养干细胞分化诱导的方法

    公开(公告)号：US20130040330A1

    公开(公告)日：2013-02-14

    申请号：US13508303

    申请日：2010-11-05

    申请人： Yoshiki Sasai ,  Mototsugu Eiraku

    发明人： Yoshiki Sasai ,  Mototsugu Eiraku

    IPC分类号： C12N5/0789 ,  C12Q1/02

    摘要： Provided is a method of inducing the differentiation of a stem cell into nerve progenitor cells, comprising the step (1) of forming a homogenous aggregate of stem cells in a serum-free medium (1) and the step (2) of suspension-culturing the homogenous aggregate of stem cells in the presence of a basement membrane reference standard.

    摘要翻译： 提供了诱导干细胞分化成神经祖细胞的方法，包括在无血清培养基（1）和悬浮培养步骤（2）中形成干细胞的均匀聚集体的步骤（1） 在存在基底膜参考标准的情况下干细胞的均匀聚集体。

    DNA encoding a tissue differentiation affecting factor
    9.
    发明授权
    DNA encoding a tissue differentiation affecting factor 失效
    编码组织分化影响因子的DNA

    公开(公告)号：US5679783A

    公开(公告)日：1997-10-21

    申请号：US343760

    申请日：1994-11-22

    申请人： Edward M. De Robertis ,  Yoshiki Sasai

    发明人： Edward M. De Robertis ,  Yoshiki Sasai

    IPC分类号： A61K38/00 ,  C07K14/475 ,  C12N15/12 ,  C07K14/46

    CPC分类号： C07K14/475 ,  A61K38/00 ,  C07K2319/00

    摘要： A functional polypeptide designated "chordin" is described that is capable of inducing dorsal (and neural tissue) development in vertebrates, and which appears to be a secreted protein. There are substantial regions of conservation with Xenopus chordin with mouse chordin, and the human gene should also be similar in those regions. A full length Xenopus cDNA for chordin is illustrated by FIGS. 1A-1F, and contains a reading frame encoding a 941 residue, 105 kDa precursor protein.

    摘要翻译： 描述了能够在脊椎动物中诱导背部（和神经组织）发育，并且其似乎是分泌蛋白质的称为“chordin”的功能性多肽。 有非常大的保护区域与爪蟾chordin与小鼠chordin，人类基因也应该是相似的那些地区。 用于chordin的全长非洲爪蟾cDNA被图示。 1A-1F，并且包含编码941残基，105kDa前体蛋白的阅读框。

    METHOD FOR CULTURE OF STEM CELL
    10.
    发明申请
    METHOD FOR CULTURE OF STEM CELL 审中-公开
    干细胞培养方法

    公开(公告)号：US20110091869A1

    公开(公告)日：2011-04-21

    申请号：US12996503

    申请日：2009-06-05

    申请人： Yoshiki Sasai ,  Takafumi Wataya ,  Mototsugu Eiraku

    发明人： Yoshiki Sasai ,  Takafumi Wataya ,  Mototsugu Eiraku

    IPC分类号： C12Q1/70 ,  C12N5/071 ,  C12N5/079 ,  C12N5/0797 ,  C12N5/0793 ,  C12Q1/68 ,  C12Q1/02

    CPC分类号： C12N5/0623 ,  C12N2501/33 ,  C12N2501/415 ,  C12N2501/42 ,  C12N2501/70 ,  C12N2506/02

    摘要： The present invention enables efficient suspension culture of stem cells in a serum-free medium by comprising a step for quickly forming a homogenous aggregate of stem cells, and provides a method of selectively inducing the differentiation of nerves from a stem cell, a method of forming a cerebral cortical nerve network in vitro, and a method of producing a steric structure of a brain tissue in vitro, as well as a method of producing hypothalamic neuron progenitor cells, comprising culturing pluripotent stem cells as a suspended aggregate in a serum-free medium that substantially does not contain a Nodal signal promoter, a Wnt signal promoter, an FGF signal promoter, a BMP signal promoter, retinoic acid and an insulin, and isolating hypothalamic neuron progenitor cells from the culture.

    摘要翻译： 本发明通过包括快速形成干细胞的均匀聚集体的步骤，能够有效地将干细胞悬浮培养在无血清培养基中，并且提供了选择性诱导神经从干细胞分化的方法，形成方法 体外的脑皮质神经网络和体外产生脑组织的立体结构的方法，以及下丘脑神经元祖细胞的制造方法，包括将多能干细胞作为悬浮骨架培养于无血清培养基中 基本上不含有Nodal信号启动子，Wnt信号启动子，FGF信号启动子，BMP信号启动子，视黄酸和胰岛素，并从培养物中分离下丘脑神经元祖细胞。