摘要:
Methods and compositions are provided for regulated expression of polypeptides in transformed bacterial hosts. A novel class of plasmid cloning vehicles includes a DNA sequence coding for the desired polypeptide (or an insertion site therefor) linked for transcriptional expression in reading phase with one or more functional fragments derived from an outer membrane protein gene of a Gram-negative bacterium. The plasmids also include an inducible promoter sequence positioned in the proper orientation for transcriptional expression of the desired polypeptide, as well as a separate DNA sequence coding for a repressor molecule which can interact with the inducible promoter to prevent transcription therefrom. Expression of the desired polypeptide is under the control of both the constitutive promoter and the inducible promoter, although transcription from either promoter is normally blocked by the repressor molecule. However, the repressor can be selectively inactivated by means of an inducer molecule to permit transcriptional expression of the desired polypeptide from both promoters. The methods utilize such plasmids to introduce genetic capability into micro-organisms for the production of proteins, such as medically or commercially useful hormones, enzymes, immunogenic proteins, or intermediates therefor, but only in the presence of an appropriate inducer.
摘要:
Methods and compositions are provided for expression of polypeptides in transformed bacterial hosts. A novel class of plasmid cloning vehicles includes a DNA sequence coding for the desired polypeptide linked in reading phase with one or more functional fragments derived from an outer membrane protein gene of a gram-negative bacterium, and also linked with an additional promoter sequence in certain cases. The methods utilize such plasmids to introduce genetic capability into microorganisms for the production of proteins, such as medically or commercially useful hormones, enzymes, immunogenic proteins, or intermediates therefor.
摘要:
Methods and compositions are provided for expression of polypeptides in transformed bacterial hosts. A novel class of plasmid cloning vehicles includes a DNA sequence coding for the desired polypeptide linked in reading phase with one or more functional fragments derived from an outer membrane protein gene of a gramnegative bacterium. The methods utilize such plasmids to introduce genetic capability into micro-organisms for the production of proteins, such as medically or commercially useful hormones, enzymes, immunogenic proteins, or intermediates therefor.
摘要:
A lightweight infrared sensor which is readily and stably erected to a substrate, the infrared sensor includes an insulating film; a first and second heat sensitive element are disposed on one surface of the insulating film separately; a first and second conductive film on one surface of the insulating film and are respectively connected to the first and the second heat sensitive element; an infrared reflection film on the other surface of the insulating film so as to face the second heat sensitive element; a reinforcing plate on which a sensor part window corresponding to a sensor part is formed and which is adhered to the insulating film; and a first and a second terminal electrode are respectively connected to the first and the second wiring film and formed on the edge of the insulating film.
摘要:
A mobile station of a two-frequency simplex radio communication system, in which communication between a base station and each mobile station is performed in such a manner that the base station continuously transmits packet data whereas each mobile station transmits burst data. The mobile station includes a radio wave propagation path condition detector for detecting the condition of a radio wave propagation path used for communication, and a communication control circuit which, when it is determined based on the condition of the radio wave propagation path detected by the radio wave propagation path condition detector, that transmission or reception timing cannot be obtained within a predetermined period of time, adaptively selects a transmission mode and notifies the base station of the selected transmission mode so as to receive data which is transmitted from the base station after the notification. This makes it possible to perform communication while maintaining a high transmission efficiency without having to perform a complicated process which needs a large amount of calculations.
摘要:
An infrared sensor comprises: an electrical insulating film sheet; first and second temperature sensor devices which are provided on one side of the electrical insulating film sheet, and are located at a distance from each other; a pair of contact electrodes, with which the first and second temperature sensor devices are attached respectively, formed on one side of the electrical insulating film sheet; an infrared absorbing film provided on the other side of the electrical insulating film sheet opposite the first temperature sensor device; and an infrared reflector film provided on the same side as the infrared absorbing film opposite the second temperature sensor device. The first and second temperature sensor devices respectively comprise: a thermistor element; and a pair of electrode layers, in which one of them is in contact with the contact electrode, formed both on the upper and lower surfaces of the thermistor element.
摘要:
The object of the invention is to provide a plant body in which the program for accumulating storage products has been modified, and applications of the same. In the invention, the plant body is constructed so as to have a B3 DNA-binding domain and an EAR motif, and so as to be capable of repressing the expression of two or more genes which code for two or more proteins having a sugar-inducible promotor function-suppressing activity.
摘要:
The personal computer adaptor device is provided with an adaptor-side signal input terminal connectable to a headphone terminal for audio signals of a personal computer, a control signal output terminal connectable to a microphone terminal of the personal computer, a plug unit connectable to an outlet jack of a power line, a digital converter for converting audio signals inputted from the adaptor-side signal input terminal into digital signals, an adaptor-side power line communication transmitting unit for transmitting audio signals via the power line connected to a plug unit, an adaptor-side power line communication receiving unit for receiving control signals of the personal computer transmitted from the output device connected to the power line via the power line connected to the plug unit, and an analog converter for converting control signals into analog signals and outputting them from the control signal output terminal.
摘要:
The present invention provides a high-safety head slider and an information storage apparatus having high operational reliability. The present invention provides a head slider equipped with a head that accesses a movable information storage medium on which information is recorded and that implements at least information reproduction, and situated close to the information storage medium. The head slider includes: a floating force generating section, opposing the information storage medium, that generates floating force upward from the information storage medium, through movement of the information storage medium; and a base section, equipped with the head, that, through floating force generated by the floating force generating section, floats from the information storage medium, the thickness in the floating direction of the base section varying along the direction of movement of the information storage medium.
摘要:
A method of checking and acknowledging reception of a message in a two-way radio communication system without causing a reduction in the efficiency of processing the message, a storage medium on which a reception checking and acknowledging program is stored for use in the two-way radio communication system, and a two-way radio communication system and a base station for use in the two-way radio communication system is provided. If message data transmitted by a terminal device is received by a base station via a relay station and an Internet network, the base station generates reception acknowledgment data and transmits it in such a manner that when downstream message data is transmitted toward a plurality of terminal devices, the above-described reception acknowledgment data is added to the downstream message data and the resultant data is transmitted to the plurality of terminal devices.