Novel cloning vehicles for polypeptide expression in microbial hosts
    1.
    发明授权
    Novel cloning vehicles for polypeptide expression in microbial hosts 失效
    用于微生物宿主中多肽表达的新型克隆载体

    公开(公告)号:US4863855A

    公开(公告)日:1989-09-05

    申请号:US378481

    申请日:1982-05-14

    CPC分类号: C12N15/62 C12N15/70

    摘要: Methods and compositions are provided for regulated expression of polypeptides in transformed bacterial hosts. A novel class of plasmid cloning vehicles includes a DNA sequence coding for the desired polypeptide (or an insertion site therefor) linked for transcriptional expression in reading phase with one or more functional fragments derived from an outer membrane protein gene of a Gram-negative bacterium. The plasmids also include an inducible promoter sequence positioned in the proper orientation for transcriptional expression of the desired polypeptide, as well as a separate DNA sequence coding for a repressor molecule which can interact with the inducible promoter to prevent transcription therefrom. Expression of the desired polypeptide is under the control of both the constitutive promoter and the inducible promoter, although transcription from either promoter is normally blocked by the repressor molecule. However, the repressor can be selectively inactivated by means of an inducer molecule to permit transcriptional expression of the desired polypeptide from both promoters. The methods utilize such plasmids to introduce genetic capability into micro-organisms for the production of proteins, such as medically or commercially useful hormones, enzymes, immunogenic proteins, or intermediates therefor, but only in the presence of an appropriate inducer.

    摘要翻译: 提供了用于在转化的细菌宿主中调节多肽表达的方法和组合物。 一类新颖的质粒克隆载体包括编码所需多肽(或其插入位点)的DNA序列,其与阅读阶段的转录表达连接,其与来自革兰氏阴性细菌的外膜蛋白基因的一种或多种功能片段连接。 质粒还包括位于适当取向的诱导型启动子序列,用于所需多肽的转录表达,以及编码可与诱导型启动子相互作用以阻止其转录的阻遏物分子的单独DNA序列。 所需多肽的表达受组成型启动子和诱导型启动子的控制,尽管来自任一启动子的转录通常被阻遏物分子阻断。 然而,阻遏物可以通过诱导剂分子选择性地失活以允许来自两个启动子的所需多肽的转录表达。 所述方法利用这种质粒将遗传能力引入微生物以产生蛋白质,例如医学或商业上有用的激素,酶,免疫原性蛋白质或其中间体,但仅在适当的诱导剂存在下。

    Novel cloning vehicles for polypeptide expression in microbial hosts
    2.
    发明授权
    Novel cloning vehicles for polypeptide expression in microbial hosts 失效
    用于微生物宿主中多肽表达的新型克隆载体

    公开(公告)号:US4666836A

    公开(公告)日:1987-05-19

    申请号:US354299

    申请日:1982-03-03

    摘要: Methods and compositions are provided for expression of polypeptides in transformed bacterial hosts. A novel class of plasmid cloning vehicles includes a DNA sequence coding for the desired polypeptide linked in reading phase with one or more functional fragments derived from an outer membrane protein gene of a gram-negative bacterium, and also linked with an additional promoter sequence in certain cases. The methods utilize such plasmids to introduce genetic capability into microorganisms for the production of proteins, such as medically or commercially useful hormones, enzymes, immunogenic proteins, or intermediates therefor.

    摘要翻译: 提供了用于在转化的细菌宿主中表达多肽的方法和组合物。 一类新颖的质粒克隆载体包括编码在阅读阶段中连接的期望多肽的DNA序列,其与来源于革兰氏阴性细菌的外膜蛋白基因的一种或多种功能性片段,并且还与一些另外的启动子序列连接 案件 所述方法利用这种质粒将遗传能力引入微生物用于生产蛋白质,例如医学或商业上有用的激素,酶,免疫原性蛋白质或其中间体。

    Novel cloning vehicles for polypeptide expression in microbial hosts
    3.
    发明授权
    Novel cloning vehicles for polypeptide expression in microbial hosts 失效
    用于微生物宿主中多肽表达的新型克隆载体

    公开(公告)号:US4624926A

    公开(公告)日:1986-11-25

    申请号:US354287

    申请日:1982-03-03

    摘要: Methods and compositions are provided for expression of polypeptides in transformed bacterial hosts. A novel class of plasmid cloning vehicles includes a DNA sequence coding for the desired polypeptide linked in reading phase with one or more functional fragments derived from an outer membrane protein gene of a gramnegative bacterium. The methods utilize such plasmids to introduce genetic capability into micro-organisms for the production of proteins, such as medically or commercially useful hormones, enzymes, immunogenic proteins, or intermediates therefor.

    摘要翻译: 提供了用于在转化的细菌宿主中表达多肽的方法和组合物。 一类新颖的质粒克隆载体包括编码在阅读阶段中连接的期望多肽的DNA序列与一种或多种衍生自革兰氏动物细菌的外膜蛋白基因的功能片段。 该方法利用这种质粒将遗传能力引入微生物以产生蛋白质,例如医学上或商业上有用的激素,酶,免疫原性蛋白质或其中间体。

    Infrared sensor
    4.
    发明授权
    Infrared sensor 有权
    红外传感器

    公开(公告)号:US09279729B2

    公开(公告)日:2016-03-08

    申请号:US14003743

    申请日:2012-03-21

    摘要: A lightweight infrared sensor which is readily and stably erected to a substrate, the infrared sensor includes an insulating film; a first and second heat sensitive element are disposed on one surface of the insulating film separately; a first and second conductive film on one surface of the insulating film and are respectively connected to the first and the second heat sensitive element; an infrared reflection film on the other surface of the insulating film so as to face the second heat sensitive element; a reinforcing plate on which a sensor part window corresponding to a sensor part is formed and which is adhered to the insulating film; and a first and a second terminal electrode are respectively connected to the first and the second wiring film and formed on the edge of the insulating film.

    摘要翻译: 红外线传感器是容易且稳定地竖立于基板的红外线传感器,其包括绝缘膜; 第一和第二热敏元件分别设置在绝缘膜的一个表面上; 绝缘膜的一个表面上的第一和第二导电膜分别连接到第一和第二热敏元件; 在所述绝缘膜的另一个表面上的与所述第二热敏元件相对的红外反射膜; 加强板,其上形成有与传感器部分相对应的传感器部分窗口,并粘附到绝缘膜上; 并且第一和第二端子电极分别连接到第一和第二布线膜并形成在绝缘膜的边缘上。

    Radio communication system and computer program product for controlling an operation of the radio communication system
    5.
    发明授权
    Radio communication system and computer program product for controlling an operation of the radio communication system 失效
    无线电通信系统和用于控制无线电通信系统的操作的计算机程序产品

    公开(公告)号:US06408189B1

    公开(公告)日:2002-06-18

    申请号:US09438423

    申请日:1999-11-12

    IPC分类号: H04B705

    摘要: A mobile station of a two-frequency simplex radio communication system, in which communication between a base station and each mobile station is performed in such a manner that the base station continuously transmits packet data whereas each mobile station transmits burst data. The mobile station includes a radio wave propagation path condition detector for detecting the condition of a radio wave propagation path used for communication, and a communication control circuit which, when it is determined based on the condition of the radio wave propagation path detected by the radio wave propagation path condition detector, that transmission or reception timing cannot be obtained within a predetermined period of time, adaptively selects a transmission mode and notifies the base station of the selected transmission mode so as to receive data which is transmitted from the base station after the notification. This makes it possible to perform communication while maintaining a high transmission efficiency without having to perform a complicated process which needs a large amount of calculations.

    摘要翻译: 一种双频单工无线电通信系统的移动台,其中基站和每个移动台之间的通信以基站连续发送分组数据的方式执行,而每个移动台发送突发数据。 移动站包括用于检测用于通信的无线电波传播路径的条件的无线电波传播路径条件检测器,以及当基于由无线电装置检测到的无线电波传播路径的条件确定的通信控制电路 波传播路径条件检测器,在预定时间段内不能获得发送或接收定时,自适应地选择发送模式,并向基站通知所选择的发送模式,以便接收从基站发送的数据 通知。 这使得可以在保持高传输效率的同时执行通信,而不必执行需要大量计算的复杂处理。

    INFRARED SENSOR
    6.
    发明申请
    INFRARED SENSOR 有权
    红外传感器

    公开(公告)号:US20120236902A1

    公开(公告)日:2012-09-20

    申请号:US13513510

    申请日:2010-12-14

    IPC分类号: G01J5/02

    摘要: An infrared sensor comprises: an electrical insulating film sheet; first and second temperature sensor devices which are provided on one side of the electrical insulating film sheet, and are located at a distance from each other; a pair of contact electrodes, with which the first and second temperature sensor devices are attached respectively, formed on one side of the electrical insulating film sheet; an infrared absorbing film provided on the other side of the electrical insulating film sheet opposite the first temperature sensor device; and an infrared reflector film provided on the same side as the infrared absorbing film opposite the second temperature sensor device. The first and second temperature sensor devices respectively comprise: a thermistor element; and a pair of electrode layers, in which one of them is in contact with the contact electrode, formed both on the upper and lower surfaces of the thermistor element.

    摘要翻译: 红外线传感器包括:电绝缘膜片; 第一和第二温度传感器装置,其设置在电绝缘膜片的一侧,并且彼此相距一定距离; 一对接触电极,分别安装有第一和第二温度传感器装置,形成在电绝缘膜片的一侧上; 设置在与第一温度传感器装置相对的电绝缘膜片的另一侧的红外线吸收膜; 以及设置在与第二温度传感器装置相对的红外线吸收膜的同一侧的红外反射膜。 第一和第二温度传感器装置分别包括:热敏电阻元件; 以及一对电极层,其中一个与接触电极接触,形成在热敏电阻元件的上表面和下表面上。

    Head slider and information storage apparatus
    9.
    发明申请
    Head slider and information storage apparatus 审中-公开
    磁头滑块和信息存储设备

    公开(公告)号:US20070008650A1

    公开(公告)日:2007-01-11

    申请号:US11284405

    申请日:2005-11-22

    IPC分类号: G11B5/60

    CPC分类号: G11B5/4826

    摘要: The present invention provides a high-safety head slider and an information storage apparatus having high operational reliability. The present invention provides a head slider equipped with a head that accesses a movable information storage medium on which information is recorded and that implements at least information reproduction, and situated close to the information storage medium. The head slider includes: a floating force generating section, opposing the information storage medium, that generates floating force upward from the information storage medium, through movement of the information storage medium; and a base section, equipped with the head, that, through floating force generated by the floating force generating section, floats from the information storage medium, the thickness in the floating direction of the base section varying along the direction of movement of the information storage medium.

    摘要翻译: 本发明提供一种具有高操作可靠性的高安全头滑块和信息存储装置。 本发明提供了一种头部滑块,其具有头部,该头部访问记录有信息的可移动信息存储介质,并且至少实现信息再现,并位于信息存储介质附近。 磁头滑块包括:浮动力产生部分,与信息存储介质相对,通过信息存储介质的移动从信息存储介质向上产生浮力; 以及配备有头的基部,通过由浮力产生部产生的浮力从信息存储介质浮动,基部的浮动方向的厚度沿着信息存储器的移动方向而变化 中。

    Method of checking and acknowledging reception of data in a two-way radio communication system
    10.
    发明授权
    Method of checking and acknowledging reception of data in a two-way radio communication system 失效
    在双向无线电通信系统中检查和确认数据接收的方法

    公开(公告)号:US06188872B1

    公开(公告)日:2001-02-13

    申请号:US09165092

    申请日:1998-10-02

    IPC分类号: H04B715

    CPC分类号: H04L1/18 H04L2001/0093

    摘要: A method of checking and acknowledging reception of a message in a two-way radio communication system without causing a reduction in the efficiency of processing the message, a storage medium on which a reception checking and acknowledging program is stored for use in the two-way radio communication system, and a two-way radio communication system and a base station for use in the two-way radio communication system is provided. If message data transmitted by a terminal device is received by a base station via a relay station and an Internet network, the base station generates reception acknowledgment data and transmits it in such a manner that when downstream message data is transmitted toward a plurality of terminal devices, the above-described reception acknowledgment data is added to the downstream message data and the resultant data is transmitted to the plurality of terminal devices.

    摘要翻译: 一种在双向无线电通信系统中检查和确认消息的接收而不会降低处理该消息的效率的方法,存储用于双向通信的接收检查和确认程序的存储介质 无线电通信系统以及用于双向无线电通信系统的双向无线电通信系统和基站。 如果终端装置发送的消息数据经由中继站和因特网网络被基站接收,则基站生成接收确认数据,并以下行消息数据向多个终端装置发送 ,将上述接收确认数据添加到下行消息数据,并将结果数据发送到多个终端设备。