公开(公告)号：US20210395796A1

公开(公告)日：2021-12-23

申请号：US17245217

申请日：2021-04-30

Applicant: Massachusetts Institute of Technology

Inventor： Fei Chen ,  Asmamaw Wassie ,  Shahar Alon ,  Adam Henry Marblestone ,  Anubhav Sinha ,  Andrew C. Payne ,  Edward Stuart Boyden

IPC: C12Q1/6806 ,  C12Q1/6841 ,  C12Q1/6811 ,  C12Q1/682 ,  C12Q1/6855

Abstract: The present invention relates to a method for amplifying at least one target RNA in a fixed and, optionally, expanded biological sample. In an embodiment of the invention, the method comprises incubating the fixed biological sample with a pair of polynucleotides complementary to non-overlapping and proximal sequences of a target RNA, wherein the polynucleotide pair hybridizes to the target RNA; ligating the polynucleotide pair using a ligase; and amplifying the ligation product. The invention further provides methods for detecting and optionally quantifying and/or sequencing the amplification product. As the method comprises hybridizing polynucleotide pairs to a target RNA in a fixed biological sample, the target RNA can be hybridized in situ.

公开(公告)号：US10995361B2

公开(公告)日：2021-05-04

申请号：US15876347

申请日：2018-01-22

Applicant: Massachusetts Institute of Technology

Inventor： Fei Chen ,  Asmamaw T. Wassie ,  Shahar Alon ,  Adam Henry Marblestone ,  Anubhav Sinha ,  Andrew Payne ,  Edward Stuart Boyden

IPC: C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6841 ,  C12Q1/6811 ,  C12Q1/682 ,  C12Q1/6855

Abstract: The present invention relates to a method for amplifying at least one target RNA in a fixed and, optionally, expanded biological sample. In an embodiment of the invention, the method comprises incubating the fixed biological sample with a pair of polynucleotides complementary to non-overlapping and proximal sequences of a target RNA, wherein the polynucleotide pair hybridizes to the target RNA; ligating the polynucleotide pair using a ligase; and amplifying the ligation product. The invention further provides methods for detecting and optionally quantifying and/or sequencing the amplification product. As the method comprises hybridizing polynucleotide pairs to a target RNA in a fixed biological sample, the target RNA can be hybridized in situ.

公开(公告)号：US20180216161A1

公开(公告)日：2018-08-02

申请号：US15876347

申请日：2018-01-22

Applicant: Massachusetts Institute of Technology

Inventor： Fei Chen ,  Asmamaw T. Wassie ,  Shahar Alon ,  Adam Henry Marblestone ,  Anubhav Sinha ,  Andrew Payne ,  Edward Stuart Boyden

IPC: C12Q1/6806 ,  C12Q1/6811 ,  C12Q1/682 ,  C12Q1/6855

CPC classification number: C12Q1/6806 ,  C12Q1/6811 ,  C12Q1/682 ,  C12Q1/6841 ,  C12Q1/6855 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2600/166 ,  C12Q2533/107 ,  C12Q2543/101

Abstract: The present invention relates to a method for amplifying at least one target RNA in a fixed and, optionally, expanded biological sample. In an embodiment of the invention, the method comprises incubating the fixed biological sample with a pair of polynucleotides complementary to non-overlapping and proximal sequences of a target RNA, wherein the polynucleotide pair hybridizes to the target RNA; ligating the polynucleotide pair using a ligase; and amplifying the ligation product. The invention further provides methods for detecting and optionally quantifying and/or sequencing the amplification product. As the method comprises hybridizing polynucleotide pairs to a target RNA in a fixed biological sample, the target RNA can be hybridized in situ.