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公开(公告)号:US20070106090A1
公开(公告)日:2007-05-10
申请号:US10572294
申请日:2004-10-18
IPC分类号: C07C309/00
CPC分类号: C07C277/08 , C07C279/12
摘要: The present invention relates to a method for synthesis of an acrylamide derivative, starting with dissolving a salt of a nucleophilic amine in water to form an aqueous solution and desalting said solution with a base, comprising the following steps: a) addition of dissolved activated acrylic acid derivative to said solution; b) acidification of aqueous phase; and c) extraction of said aqueous phase. In a preferred embodiment the acryl amide derivative is an immobiline, preferably acrylamido agmatine. The preferred use of the immobilines is for production of 2D gels.
摘要翻译: 本发明涉及一种合成丙烯酰胺衍生物的方法,首先将亲核胺的盐溶解在水中形成水溶液,并用碱脱盐所述溶液,包括以下步骤:a)加入溶解的活化丙烯酸 酸衍生物至所述溶液; b)水相酸化; 和c)提取所述水相。 在优选的实施方案中,丙烯酰胺衍生物是固定化物,优选丙烯酰胺基丁胺。 固定装置的优选用途是生产2D凝胶。
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公开(公告)号:US07294743B2
公开(公告)日:2007-11-13
申请号:US10572294
申请日:2004-10-18
IPC分类号: C07C233/10
CPC分类号: C07C277/08 , C07C279/12
摘要: The present invention relates to a method for synthesis of an acrylamide derivative, starting with dissolving a salt of a nucleophilic amine in water to form an aqueous solution and desalting said solution with a base, comprising the following steps: a) addition of dissolved activated acrylic acid derivative to said solution; b) acidification of aqueous phase; and c) extraction of said aqueous phase.
摘要翻译: 本发明涉及一种合成丙烯酰胺衍生物的方法,首先将亲核胺的盐溶解在水中形成水溶液,并用碱脱盐所述溶液,包括以下步骤:a)加入溶解的活化丙烯酸 酸衍生物至所述溶液; b)水相酸化; 和c)提取所述水相。
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公开(公告)号:US20080199959A1
公开(公告)日:2008-08-21
申请号:US11917169
申请日:2006-06-19
申请人: Mattias Algotsson , Gunnar Glad , Nicolas Thevenin
发明人: Mattias Algotsson , Gunnar Glad , Nicolas Thevenin
CPC分类号: C12N5/0075 , G01N33/5005 , G01N33/5432
摘要: The present invention relates to a method for cell culture, more precisely small scale cell culture. In the present invention a screening tool is used which comprises particulate matter or microcarriers, such as beads, attached to a solid support, such as a microtiter plate, for the cultivation of cells on said microcarriers. The microcarriers are preferably cultivation beads, such as CYTODEX™. According to the invention, this small scale format for cell cultivation may be used for any testing involving cells, for example testing of optimal growth conditions for a specific type of cell, such as stem cells. Another use is cell expansion.
摘要翻译: 本发明涉及一种细胞培养方法,更准确地说是小规模细胞培养。 在本发明中,使用了一种筛选工具,其包括连接到固体支持物例如微量滴定板上的颗粒物质或微载体,例如珠,用于在所述微载体上培养细胞。 微载体优选是培养珠,例如CYTODEX TM。 根据本发明,用于细胞培养的这种小规模格式可用于涉及细胞的任何测试,例如测试特定类型细胞如干细胞的最佳生长条件。 另一个用途是细胞扩增。
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公开(公告)号:US07750129B2
公开(公告)日:2010-07-06
申请号:US10589717
申请日:2005-02-25
申请人: Bo-Lennart Johansson , Hans J. Johansson , Anna Grönberg , Jean-Luc Maloisel , Nicolas Thevenin
发明人: Bo-Lennart Johansson , Hans J. Johansson , Anna Grönberg , Jean-Luc Maloisel , Nicolas Thevenin
CPC分类号: B01J20/3253 , B01D15/3847 , B01J20/3242 , B01J20/3246 , B01J20/3255 , B01J20/3285
摘要: The present invention relates to a process for the purification of antibodies from one or more impurities in a liquid, which process comprises contacting said liquid with a first chromatography resin comprised of a support to which multi-modal ligands have been immobilised to adsorb the antibodies to the resin, wherein each multi-modal ligand comprises at least one cation-exchanging group and at least one aromatic or heteroaromatic ring system; adding an eluent to release the antibodies from the resin; and contacting the eluate so obtained with a second chromatography resin. In one embodiment, the ring-forming atoms of the aromatic or heteroaromatic entity are selected from the group consisting of C, S and O, and the cation exchanging group is a weak cation exchanger.
摘要翻译: 本发明涉及一种从液体中的一种或多种杂质纯化抗体的方法,该方法包括使所述液体与包含多元配体的载体的第一层析树脂接触以将抗体吸附到 所述树脂,其中每个多模式配体包含至少一个阳离子交换基团和至少一个芳族或杂芳族环系统; 添加洗脱液以从树脂中释放抗体; 并将如此获得的洗脱液与第二层析树脂接触。 在一个实施方案中,芳族或杂芳族实体的成环原子选自C,S和O,阳离子交换基团是弱阳离子交换剂。
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公开(公告)号:US07510657B2
公开(公告)日:2009-03-31
申请号:US10547569
申请日:2004-03-05
CPC分类号: B01J20/286 , B01D15/3804 , B01J20/321 , B01J20/3212 , B01J20/3219 , B01J20/3251 , B01J20/3255 , B01J20/3259
摘要: The present invention is a method of preparing affinity ligands, which method comprises to provide a cyclic scaffold comprising a thiol group, a carbonyl group and an amine group; to derivatize the amine group of said scaffold with an electrophile that carries either a functionality capable of affinity interaction or a functionality capable of a second interaction with a target molecule; and to open up the derivatized scaffold and add an amine that carries either a functionality capable of a second interaction or a functionality capable of affinity interaction with a target molecule. The method provides provide affinity ligands, which are capable of affinity interaction as well as a second kind of interaction.
摘要翻译: 本发明是制备亲和配体的方法,该方法包括提供包含硫醇基,羰基和胺基的环状支架; 以亲电子试剂衍生所述支架的胺基团,所述亲电子试剂携带能够进行亲和相互作用的官能团或能够与靶分子进行第二相互作用的官能团; 并开启衍生的支架并加入携带能够进行第二相互作用的官能团或能够与靶分子亲和相互作用的官能团的胺。 该方法提供了提供亲和力配体,其能够进行亲和力相互作用以及第二种相互作用。
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公开(公告)号:US20080179248A1
公开(公告)日:2008-07-31
申请号:US11995979
申请日:2006-07-24
申请人: Andreas Axen , Anders Hagvall , Anders Larsson , Nicolas Thevenin
发明人: Andreas Axen , Anders Hagvall , Anders Larsson , Nicolas Thevenin
CPC分类号: B01D67/0093 , B01D71/14 , B01D2323/30 , B01J20/24 , B01J20/28033 , B01J20/286 , B01J20/288 , B01J20/30 , B01J20/3219 , B01J20/3244 , B01J2220/4825 , C08B15/005 , C08J5/2287 , C08J2301/10
摘要: The invention relates to a process for making porous cross-linked cellulose membranes and processes for coupling a chromatography ligand to cross-linked cellulose membranes. The invention provides methods for separating a first component from a second component in a solution based upon a difference in the size of the first and second components, and methods for separating target molecules from other components in a solution comprising use of membranes obtainable by the process of the invention. The method has particular utility in separating proteins from cell lysates and cultures.
摘要翻译: 本发明涉及制备多孔交联纤维素膜的方法和将色谱配体偶联到交联的纤维素膜上的方法。 本发明提供了基于第一和第二组分的尺寸差异将溶液中的第一组分与第二组分分离的方法,以及用于在溶液中从其它组分分离目标分子的方法,所述方法包括使用可通过该方法获得的膜 的本发明。 该方法在从细胞裂解物和培养物中分离蛋白质方面具有特殊的用途。
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公开(公告)号:US08648146B2
公开(公告)日:2014-02-11
申请号:US13489492
申请日:2012-06-06
申请人: Gunnar Glad , Jean-Luc Maloisel , Nicolas Thevenin
发明人: Gunnar Glad , Jean-Luc Maloisel , Nicolas Thevenin
IPC分类号: C08F8/00 , C07H1/00 , C07D303/02
CPC分类号: B01J20/262 , B01J20/261 , B01J20/265 , B01J20/267 , B01J20/28016 , B01J20/286 , B01J20/287 , B01J20/3204 , B01J20/321 , B01J20/3212 , B01J20/3217 , B01J20/3219 , B01J20/3246 , B01J20/3248 , B01J20/3251 , B01J20/3253 , B01J20/3255 , B01J2220/54 , B01J2220/58
摘要: Disclosed is a method for activating a solid support material with epoxy groups and for immobilizing ligands thereon, utilizing phase transfer catalytic conditions. The method permits the introduction of epoxy groups and specific nucleophilic ligands on the support material with a high level of substitution. Furthermore, the invention provides a general method for immobilizing a ligand for use in a wide variety of chromatographic separation procedures such as ion exchange chromatography, hydrophobic interaction chromatography (HIC), reverse phase chromatography (RPC), or affinity chromatography.
摘要翻译: 公开了一种利用相转移催化条件活化具有环氧基团的固体支持材料和固定配体的方法。 该方法允许以高水平的取代在载体材料上引入环氧基团和特定的亲核配体。 此外,本发明提供了用于固定配体以用于各种色谱分离方法如离子交换层析,疏水相互作用层析(HIC),反相色谱(RPC)或亲和层析中的一般方法。
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公开(公告)号:US08496123B2
公开(公告)日:2013-07-30
申请号:US11995979
申请日:2006-07-24
申请人: Andreas Axen , Anders Hagvall , Anders Larsson , Nicolas Thevenin
发明人: Andreas Axen , Anders Hagvall , Anders Larsson , Nicolas Thevenin
CPC分类号: B01D67/0093 , B01D71/14 , B01D2323/30 , B01J20/24 , B01J20/28033 , B01J20/286 , B01J20/288 , B01J20/30 , B01J20/3219 , B01J20/3244 , B01J2220/4825 , C08B15/005 , C08J5/2287 , C08J2301/10
摘要: The invention relates to a process for making porous cross-linked cellulose membranes and processes for coupling a chromatography ligand to cross-linked cellulose membranes. The invention provides methods for separating a first component from a second component in a solution based upon a difference in the size of the first and second components, and methods for separating target molecules from other components in a solution comprising use of membranes obtainable by the process of the invention. The method has particular utility in separating proteins from cell lysates and cultures.
摘要翻译: 本发明涉及制备多孔交联纤维素膜的方法和将色谱配体偶联到交联的纤维素膜上的方法。 本发明提供了基于第一和第二组分的尺寸差异将溶液中的第一组分与第二组分分离的方法,以及用于在溶液中从其它组分分离目标分子的方法,所述方法包括使用可通过该方法获得的膜 的本发明。 该方法在从细胞裂解物和培养物中分离蛋白质方面具有特殊的用途。
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公开(公告)号:US07320754B2
公开(公告)日:2008-01-22
申请号:US10547567
申请日:2004-03-05
IPC分类号: B01D15/08
CPC分类号: B01J20/3242 , B01D15/363 , B01J20/289 , B01J20/3219 , B01J20/3285 , B01J41/12 , B01J41/20
摘要: The invention relates to a method of preparing multi-modal anion-exchange ligands, which comprises providing a cyclic three-functional scaffold comprising an amine, a carbonyl and a thiol; optionally derivatisation of the amine of the scaffold to provide an anion-exchanging group and/or to protect the amine; and aminolysis to open up the derivative by adding a reagent comprising an amine coupled to a residue R in order to add said amine to the carbonyl carbon of the opened scaffold. The scaffold is advantageously homocysteine thiolactone. In one embodiment, the method comprises an additional step of immobilising the opened scaffold to a base matrix to provide a separation medium, such as a chromatography medium.
摘要翻译: 本发明涉及制备多模式阴离子交换配体的方法,其包括提供包含胺,羰基和硫醇的环状三官能支架; 可任选地衍生化支架的胺以提供阴离子交换基团和/或保护胺; 并通过加入包含偶联到残基R的胺的试剂以将所述胺加入到开放的支架的羰基碳中,开启衍生物。 支架有利的是同型半胱氨酸硫内酯。 在一个实施方案中,该方法包括将开放的支架固定在基础基质上以提供分离介质如色谱介质的附加步骤。
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公开(公告)号:US20060175237A1
公开(公告)日:2006-08-10
申请号:US10547567
申请日:2004-03-05
IPC分类号: B01D15/08
CPC分类号: B01J20/3242 , B01D15/363 , B01J20/289 , B01J20/3219 , B01J20/3285 , B01J41/12 , B01J41/20
摘要: The invention relates to a method of preparing multi-modal anion-exchange ligands, which comprises providing a cyclic three-functional scaffold comprising an amine, a carbonyl and a thiol; optionally derivatisation of the amine of the scaffold to provide an anion-exchanging group and/or to protect the amine; and aminolysis to open up the derivative by adding a reagent comprising an amine coupled to a residue R in order to add said amine to the carbonyl carbon of the opened scaffold. The scaffold is advantageously homocysteine thiolactone. In one embodiment, the method comprises an additional step of immobilising the opened scaffold to a base matrix to provide a separation medium, such as a chromatography medium.
摘要翻译: 本发明涉及制备多模式阴离子交换配体的方法,其包括提供包含胺,羰基和硫醇的环状三官能支架; 可任选地衍生化支架的胺以提供阴离子交换基团和/或保护胺; 并通过加入包含偶联到残基R的胺的试剂以将所述胺加入到开放的支架的羰基碳中,开启衍生物。 支架有利的是同型半胱氨酸硫内酯。 在一个实施方案中,该方法包括将开放的支架固定在基础基质上以提供分离介质如色谱介质的附加步骤。
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