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    Lepidopteran AKH signal sequence
    2.
    发明授权
    Lepidopteran AKH signal sequence 失效
    鳞翅目AKH信号序列

    公开(公告)号：US5023328A

    公开(公告)日：1991-06-11

    申请号：US389377

    申请日：1989-08-04

    申请人： Max D. Summers ,  James Y. Bradfield ,  Larry L. Keeley

    发明人： Max D. Summers ,  James Y. Bradfield ,  Larry L. Keeley

    IPC分类号： C07K14/575 ,  C07K14/73 ,  C12N15/16 ,  C12N15/866

    CPC分类号： C12N15/86 ,  C07K14/575 ,  C07K14/70514 ,  C07K2319/02 ,  C12N2710/14143

    摘要： A baculovirus expression vector system is constructed wherein the natural signal DNA peptide sequence associated with the desired foreign gene, such as CD4 (T cell surface protein T4) is replaced by the signal DNA sequence from the Lepidopteran adipokinetic hormone (AKH) precursor signal peptides. The exemplary Manduca sexta AKH signal sequence is represented as follows: ##STR1## .

    摘要翻译： 构建杆状病毒表达载体系统，其中与期望的外源基因（例如CD4（T细胞表面蛋白质T4））相关的天然信号DNA肽序列被来自鳞翅目脂肪动力激素（AKH）前体信号肽的信号DNA序列替代。 示例性的Manduca sexta AKH信号序列表示如下：5'。 。 。 ATG-TAC-AAG-CTC-ACA-GTC-TTC-CTG-ATG-TTC-Met-Tyr-Lys-Leu-Thr-Val-Phe-Leu-Met-Phe-ATC-GCT-TTC-GTC-ATC- ATC-GCT-GAG-GCC。 。 。 3'Ile-Ala-Phe-Val-Ile-Ile-Ala-Glu-Ala。

    Method to improve the efficiency of processing and secretion of foreign genes in insect systems
    3.
    发明授权
    Method to improve the efficiency of processing and secretion of foreign genes in insect systems 失效
    提高昆虫系统中外来基因加工和分泌效率的方法

    公开(公告)号：US5278050A

    公开(公告)日：1994-01-11

    申请号：US892757

    申请日：1992-06-03

    申请人： Max D. Summers

    发明人： Max D. Summers

    IPC分类号： C07K14/435 ,  C07K14/73 ,  C12N15/866 ,  C12P21/02 ,  C12P21/00

    CPC分类号： C12N15/86 ,  C07K14/43563 ,  C07K14/70514 ,  C07K2319/02 ,  C12N2710/14143

    摘要： The engineering of foreign vertebrate gene constructs by recombinant DNA techniques for the more efficient processing and secretion of foreign genes in insect systems is done by replacing the foreign vertebrate protein signal peptide sequences with protein signal sequences from insect cell secreted proteins. A baculovirus expression vector system is constructed wherein the natural signal DNA peptide sequence associated with the desired foreign gene, such as CD4 (T cell surface protein T4) is replaced by the signal DNA sequence from the insect signal peptides coding for the cuticle gene or adipokinetic hormone.

    摘要翻译： 通过用昆虫细胞分泌的蛋白质的蛋白质信号序列替换外来的脊椎动物蛋白质信号肽序列，通过重组DNA技术对外来基因在昆虫系统中更有效的加工和分泌进行外源脊椎动物基因构建体的工程化。 构建杆状病毒表达载体系统，其中与期望的外源基因（例如CD4（T细胞表面蛋白质T4））相关的天然信号DNA肽序列被来自编码角质层基因或脂肪动力学的昆虫信号肽的信号DNA序列替代 激素。

    Method for producing a recombinant baculovirus expression vector
    4.
    发明授权
    Method for producing a recombinant baculovirus expression vector 失效
    重组杆状病毒表达载体的制备方法

    公开(公告)号：US4879236A

    公开(公告)日：1989-11-07

    申请号：US40367

    申请日：1987-04-16

    申请人： Gale E. Smith ,  Max D. Summers

    发明人： Gale E. Smith ,  Max D. Summers

    IPC分类号： C07K14/01 ,  C07K14/52 ,  C12N7/01 ,  C12N15/83 ,  C12N15/866

    CPC分类号： C12N15/86 ,  C07K14/005 ,  C07K14/52 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/09 ,  C07K2319/61 ,  C07K2319/735 ,  C07K2319/75 ,  C12N2710/14122 ,  C12N2710/14143

    摘要： A method for producng a recombinant baculovirus expression vector, capable of expressing a selected gene in a host insect cell, is disclosed. The method involves cleaving baculovirus DNA to produce a DNA fragment comprising a polyhedrin gene or portion thereof, including a polyhedrin promoter. A recombinant transfer vector is prepared by inserting said DNA fragment into a cloning vehicle and thereafter inserting a selected gene into the thus modified cloning vehicle such that it is under the transcriptional control of the polyhedrin promoter. The recombinant transfer vector is contacted with a baculovirus DNA so as to effect recombination and incorporation of the selected gene into the baculovirus genome. The resultant recombinant baculovirus is then used to infect susceptible insects or cultured insect cells and the protein product from the incorporated selected gene is produced from the infection.

    摘要翻译： 公开了一种能够在宿主昆虫细胞中表达选定基因的重组杆状病毒表达载体的生产方法。 该方法包括切断杆状病毒DNA以产生包含多角体蛋白基因或其部分的DNA片段，包括多角体蛋白启动子。 通过将所述DNA片段插入到克隆载体中，然后将所选择的基因插入如此修饰的克隆载体中，使其处于多角体启动子的转录控制下来制备重组转移载体。 将重组转移载体与杆状病毒DNA接触，以便将所选择的基因重组并并入杆状病毒基因组中。 然后将所得重组杆状病毒用于感染易感昆虫或培养的昆虫细胞，并且从感染引起来自掺入的所选基因的蛋白质产物。

    Baculovirus dual promoter expression vector
    6.
    发明授权
    Baculovirus dual promoter expression vector 失效
    BACULOVIRUS双重促进剂表达载体

    公开(公告)号：US5169784A

    公开(公告)日：1992-12-08

    申请号：US583392

    申请日：1990-09-17

    申请人： Max D. Summers ,  Christian E. G. Oker-Blom

    发明人： Max D. Summers ,  Christian E. G. Oker-Blom

    IPC分类号： A61K39/00 ,  A61K39/20 ,  C07K14/18 ,  C07K14/19 ,  C12N7/00 ,  C12N15/09 ,  C12N15/40 ,  C12N15/86 ,  C12N15/866

    CPC分类号： C12N15/86 ,  C07K14/005 ,  C12N7/00 ,  A61K39/00 ,  C12N2710/14121 ,  C12N2710/14143 ,  C12N2710/14144 ,  C12N2770/36122 ,  C12N2770/36143 ,  C12N2770/36222

    摘要： This invention relates to specifically designing and genetically engineering recombinant baculovirus for producing, in a compatible insect system, a desired protein, virus, protein hybrid, or virus hybrid. In particular aspects, this invention relates to the use of different baculovirus promoters for the ultimate purpose of constructing a recombinant baculovirus designed for the investigator's specific need. For example, the recombinant baculovirus of this invention can be designed to produce a viral pesticide.This invention also describes the construction of a genetically engineered virus or virus hybrid (e.g. animal or human pathogen) which is not capable of replicating itself but is essentially identical to the authentic pathogen in terms of structure and antigenicity. This baculovirus is constructed such that the non-structural viral genes are truncated, mutated or both and are located 3' and directly under the control of an early baculovirus gene promoter and the structural viral genes are located 3' and directly under the control of a late baculovirus gene promoter. This genetically engineered baculovirus is therefore capable of temporal regulation and successive synthesis of non-structural and structural proteins. The truncated or mutated non-structural viral genes creates the non-replicative aspect of this invention. Since the genetically produced virus or virus hybrid is essentially identical to the authentic pathogen, the product is thereby highly antigenic and potent in terms of efficacy and efficiency. This invention enables the design and constructure of a virus particle or virus hybrid with specific antigenic properties which further allows for the safe and inexpensive production of vaccines or diagnostics.

    摘要翻译： 本发明涉及专门设计和遗传工程重组杆状病毒，用于在相容的昆虫系统中产生所需的蛋白质，病毒，蛋白质杂种或病毒杂交体。 在具体方面，本发明涉及不同杆状病毒启动子用于构建针对研究者特定需要而设计的重组杆状病毒的最终目的。 例如，本发明的重组杆状病毒可被设计成生产病毒性农药。 本发明还描述了在结构和抗原性方面不能复制自身但与真实病原体基本相同的遗传工程病毒或病毒杂交体（例如动物或人类病原体）的构建。 构建这种杆状病毒使得非结构病毒基因被截短，突变或两者并且位于3'并且直接在早期杆状病毒基因启动子的控制下，并且结构病毒基因位于3'并且直接在a 晚期杆状病毒基因启动子。 因此，这种基因工程的杆状病毒能够进行非结构和结构蛋白质的时间调节和连续合成。 截短或突变的非结构病毒基因产生本发明的非复制方面。 由于遗传产生的病毒或病毒杂合物与真实病原体基本上相同，因此产品在功效和效率方面具有高度的抗原性和有效性。 本发明使得具有特定抗原性质的病毒颗粒或病毒杂交体的设计和结构进一步允许安全和廉价地生产疫苗或诊断。

    Insect signal sequences useful to improve the efficiency of processing and secretion of foreign genes in insect systems
    7.
    发明授权
    Insect signal sequences useful to improve the efficiency of processing and secretion of foreign genes in insect systems 失效
    昆虫信号序列有助于提高昆虫系统中外来基因的加工和分泌效率

    公开(公告)号：US5155037A

    公开(公告)日：1992-10-13

    申请号：US389376

    申请日：1989-08-04

    申请人： Max D. Summers

    发明人： Max D. Summers

    IPC分类号： C07K14/435 ,  C07K14/73 ,  C12N15/866

    CPC分类号： C12N15/86 ,  C07K14/43563 ,  C07K14/70514 ,  C07K2319/02 ,  C12N2710/14143

    摘要： The engineering of foreign vertebrate gene constructs by recombinant DNA techniques for the more efficient processing and secretion of foreign genes in insect systems is done by replacing the foreign vertebrate protein signal peptide sequences with protein signal sequences from insect cell secreted proteins. A baculovirus expression vector system is constructed wherein the natural signal DNA peptide sequence associated with the desired foreign gene, such as CD4 (T cell surface protein T4) is replaced by the signal DNA sequence from the insect signal peptides coding for the cuticle gene or adipokinetic hormone.

    摘要翻译： 通过用昆虫细胞分泌的蛋白质的蛋白质信号序列替换外来的脊椎动物蛋白质信号肽序列，通过重组DNA技术对外来基因在昆虫系统中更有效的加工和分泌进行外源脊椎动物基因构建体的工程化。 构建杆状病毒表达载体系统，其中与期望的外源基因（例如CD4（T细胞表面蛋白质T4））相关的天然信号DNA肽序列被来自编码角质层基因或脂肪动力学的昆虫信号肽的信号DNA序列替代 激素。

    Method for producing a recombinant baculovirus expression vector
    8.
    发明授权
    Method for producing a recombinant baculovirus expression vector 失效
    重组杆状病毒表达载体的制备方法

    公开(公告)号：US4745051A

    公开(公告)日：1988-05-17

    申请号：US498858

    申请日：1983-05-27

    申请人： Gale E. Smith ,  Max D. Summers

    发明人： Gale E. Smith ,  Max D. Summers

    IPC分类号： C07K14/01 ,  C07K14/52 ,  C12N15/00 ,  C12N15/866 ,  C12P21/00 ,  C07H21/04 ,  C12N1/00 ,  C12N7/00 ,  C12P19/34

    CPC分类号： C12N15/86 ,  C07K14/005 ,  C07K14/52 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/09 ,  C07K2319/735 ,  C07K2319/75 ,  C12N2710/14122 ,  C12N2710/14143

    摘要： A method for producing a recombinant baculovirus expression vector, capable of expression of a selected gene in a host insect cell, is disclosed. The preferred method involves cleaving baculovirus DNA to produce a DNA fragment comprising a polyhedrin gene or portion thereof, including a polyhedrin promoter. A recombinant shuttle vector is prepared by inserting said DNA fragment into a cloning vehicle and thereafter inserting a selected gene into the thus modified cloning vehicle such that it is under the transcriptional control of the polyhedrin promoter. The recombinant shuttle vector is contacted with a baculovirus DNA so as to effect recombination and incorporation of the selected gene into the baculovirus genome.

    摘要翻译： 公开了能够在宿主昆虫细胞中表达选定基因的重组杆状病毒表达载体的制备方法。 优选的方法包括切断杆状病毒DNA以产生包含多角体蛋白基因或其部分的DNA片段，包括多角体蛋白启动子。 通过将所述DNA片段插入克隆载体中，然后将所选择的基因插入到如此修饰的克隆载体中使得其处于多角体蛋白启动子的转录控制下来制备重组穿梭载体。 将重组穿梭载体与杆状病毒DNA接触，以便将所选择的基因重组并并入杆状病毒基因组中。