公开(公告)号：US20080197023A1

公开(公告)日：2008-08-21

申请号：US11573081

申请日：2005-06-16

申请人： Seiichiro Miyahara ,  Chiaki Okumura ,  Naoki Fukui ,  Megumi Akamatsu

发明人： Seiichiro Miyahara ,  Chiaki Okumura ,  Naoki Fukui ,  Megumi Akamatsu

IPC分类号： G01N27/26 ,  G01N33/487

CPC分类号： G01N15/1031 ,  C12Q1/06 ,  C12Q1/10

摘要： A method of measuring the number of bacteria according to the present invention includes step (a) through step (d). First, in step (a), a to-be-measured sample (specimen) including a predetermined bacterial strain (such as Escherichia coli or coliform bacteria) is added to a predetermined medium (such as a medium used for a specific enzyme substrate culture medium method). In step (b), a current value flowing through the medium added with the sample is measured with an oxygen electrode at a predetermined temperature and at a predetermined constant voltage. In step (c), time required is measured for the current value that has decreased temporarily after starting the measurement of step (b) to increase thereafter to exceed a predetermined threshold value. In step (d), the initial number of bacteria of the bacterial strain included in the sample is calculated based on the time required.

摘要翻译： 根据本发明的测量细菌数量的方法包括步骤（a）至步骤（d）。 首先，在步骤（a）中，向规定的培养基（例如用于特定的酶底物培养基的培养基）中加入包含规定菌种（例如大肠杆菌或大肠菌群）的待测样品（标本） 中等方法）。 在步骤（b）中，用氧电极在预定温度和预定的恒定电压下测量流过添加了样品的介质的电流值。 在步骤（c）中，测量在开始步骤（b）的测量之后临时减小的电流值随后增加到超过预定阈值的时间。 在步骤（d）中，根据所需的时间计算样品中包含的细菌菌株的初始菌数。

公开(公告)号：US20080057535A1

公开(公告)日：2008-03-06

申请号：US11791312

申请日：2005-11-22

申请人： Megumi Akamatsu ,  Chiaki Okumura ,  Seiichiro Miyahara ,  Naoki Fukui

发明人： Megumi Akamatsu ,  Chiaki Okumura ,  Seiichiro Miyahara ,  Naoki Fukui

IPC分类号： C12Q1/06 ,  C12M1/34

CPC分类号： C12Q1/06

摘要： Various settings used in a process proceeding in line with a flow chart are reset. An oxygen current is measured. A slope of the oxygen current is obtained. A determination is made as to whether or not the slope can be considered to become stable. A first measuring time is determined. In order to determine the stability of the slope, the oxygen current is sequentially measured and the slope of the oxygen current is updated until the slope is determined to become stable. When the slope is determined to become stable, the first measuring time is determined. When the slope is not determined to become stable, the oxygen current is further measured.

摘要翻译： 根据流程图进行的过程中使用的各种设置被重置。 测量氧气电流。 得到氧气电流的斜率。 确定斜率是否可以被认为变得稳定。 确定第一测量时间。 为了确定斜率的稳定性，依次测量氧气电流，并更新氧气电流的斜率，直到确定斜率变得稳定为止。 当确定斜率变得稳定时，确定第一测量时间。 当斜率未确定为稳定时，进一步测量氧气电流。

公开(公告)号：US07955493B2

公开(公告)日：2011-06-07

申请号：US11573081

申请日：2005-06-16

申请人： Seiichiro Miyahara ,  Chiaki Okumura ,  Naoki Fukui ,  Megumi Akamatsu

发明人： Seiichiro Miyahara ,  Chiaki Okumura ,  Naoki Fukui ,  Megumi Akamatsu

IPC分类号： C12M1/34

CPC分类号： G01N15/1031 ,  C12Q1/06 ,  C12Q1/10

摘要： A method of measuring the number of bacteria according to the present invention includes step (a) through step (d). First, in step (a), a to-be-measured sample (specimen) including a predetermined bacterial strain (such as Escherichia coli or coliform bacteria) is added to a predetermined medium (such as a medium used for a specific enzyme substrate culture medium method). In step (b), a current value flowing through the medium added with the sample is measured with an oxygen electrode at a predetermined temperature and at a predetermined constant voltage. In step (c), time required is measured for the current value that has decreased temporarily after starting the measurement of step (b) to increase thereafter to exceed a predetermined threshold value. In step (d), the initial number of bacteria of the bacterial strain included in the sample is calculated based on the time required.

摘要翻译： 根据本发明的测量细菌数量的方法包括步骤（a）至步骤（d）。 首先，在步骤（a）中，向规定的培养基（例如用于特定的酶底物培养基的培养基）中加入包含规定菌种（例如大肠杆菌或大肠菌群）的待测样品（标本） 中等方法）。 在步骤（b）中，用氧电极在预定温度和预定的恒定电压下测量流过添加了样品的介质的电流值。 在步骤（c）中，测量在开始步骤（b）的测量之后临时减小的电流值随后增加到超过预定阈值的时间。 在步骤（d）中，根据所需的时间计算样品中包含的细菌菌株的初始菌数。

公开(公告)号：US20060040339A1

公开(公告)日：2006-02-23

申请号：US10919290

申请日：2004-08-17

申请人： Naoki Fukui ,  Megumi Akamatsu ,  Seiichiro Miyahara ,  Chiaki Okumura ,  Toshimi Murata ,  Yoshihisa Amano

发明人： Naoki Fukui ,  Megumi Akamatsu ,  Seiichiro Miyahara ,  Chiaki Okumura ,  Toshimi Murata ,  Yoshihisa Amano

IPC分类号： G06F19/00 ,  C12Q1/04

CPC分类号： C12Q1/06

摘要： A method of constructing a calibration curve in a determination of microbial counts is provided. An immunoassay of the present invention is characterized in that samples of a specimen material are prepared so that microbial counts therein are altered.

摘要翻译： 提供了一种在确定微生物计数中构建校准曲线的方法。 本发明的免疫测定法的特征在于，准备样品的样品，使其中的微生物计数改变。

公开(公告)号：US20090191614A1

公开(公告)日：2009-07-30

申请号：US10585612

申请日：2004-07-30

申请人： Seiichiro Miyahara

发明人： Seiichiro Miyahara

IPC分类号： C12M1/38

CPC分类号： B01L7/00 ,  B01L2200/147 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1844 ,  C12M41/12 ,  C12M41/48 ,  G01K1/20

摘要： The present invention relates to a temperature control device, and has an object to accurately control temperatures of microorganisms or cells when for example applied to culture and so on of the microorganisms or cells, for example. The temperature control device includes a plurality of cell parts (2) holding microorganisms or cells, a heater (1), and a cooling unit (7). The heater (1) is provided around the cell parts (2) to heat the cell parts (2). The cooling unit (7) includes a cooling fan (71), a cooling fin (72), an aluminum conduction block (73), a Peltier element (74), a radiating fin (75), and a radiating fan (76). Air (701) is supplied to the cooling fin (72) via the cooling fan (71). The air (701) is cooled in the cooling fin (72) because heat is moved from the air (701) to the cooling (72). Air (702) having been cooled is sent to the cell parts (2) to cool the cell parts (2). Temperatures inside the cell parts are controlled by controlling operations of the heater (1) and the cooling unit (7).

摘要翻译： 温度控制装置技术领域本发明涉及一种温度控制装置，其目的在于，例如适用于微生物或细胞等的培养等时，可以精确地控制微生物或细胞的温度。 温度控制装置包括容纳微生物或细胞的多个细胞部分（2），加热器（1）和冷却单元（7）。 加热器（1）设置在电池部分（2）周围以加热电池部件（2）。 冷却单元（7）包括冷却风扇（71），冷却翅片（72），铝传导块（73），珀耳帖元件（74），散热片（75）和散热风扇（76） 。 空气（701）通过冷却风扇（71）供给到散热片（72）。 由于热量从空气（701）移动到冷却（72），所以空气（701）在冷却翅片（72）中被冷却。 已经冷却的空气（702）被送到电池部件（2）以冷却电池部件（2）。 通过控制加热器（1）和冷却单元（7）的操作来控制电池部件内的温度。

公开(公告)号：US08242373B2

公开(公告)日：2012-08-14

申请号：US12227365

申请日：2007-06-06

申请人： Seiichiro Miyahara

发明人： Seiichiro Miyahara

IPC分类号： H05K1/00

CPC分类号： H05K1/025 ,  H05K1/028 ,  H05K2201/09263 ,  H05K2201/09281

摘要： A flexible wiring board is provided having a wiring structure which can reduce transmission loss by reducing impedance mismatching even if being folded in a three-dimensional manner. In a flexible wiring board 10 having a characteristic impedance control circuit 20, the flexible wiring board has a planar projection shape of a folded spot 20A in the characteristic impedance control circuit after folding in an arc state along a tangent.

摘要翻译： 提供了具有布线结构的柔性布线板，即使以三维方式折叠，也可以通过降低阻抗失配来降低传输损耗。 在具有特性阻抗控制电路20的柔性布线板10中，柔性布线板在沿着切线折叠成弧状态之后，在特性阻抗控制电路中具有折叠点20A的平面投影形状。

公开(公告)号：US07634330B2

公开(公告)日：2009-12-15

申请号：US11569772

申请日：2005-04-08

申请人： Seiichiro Miyahara

发明人： Seiichiro Miyahara

IPC分类号： G05D23/00 ,  C12M1/00 ,  F27B1/26 ,  F27D15/02

CPC分类号： G05D23/1934 ,  B01L3/50851 ,  B01L7/00 ,  C12M41/12 ,  C12M41/48

摘要： An object of the present invention is to accurately control temperatures of microorganisms or the like. In the present invention, a temperature controller comprises a plurality of cells for storing microorganisms or the like, heaters, and a cooling section. The heaters selectively heat the plurality of cells, and the cooling section wholly cools the plurality of cells. When the maximum value of temperature of a plurality of places is not lower than a first lower limit, the cooling section is driven. When a temperature of one place is not higher than a first lower limit, the heater to heat the place is driven. When the maximum value is not higher than a second lower limit, the cooling section is stopped. When a temperature of one place is not lower than a second upper limit, the heater to heat the place is not driven.

摘要翻译： 本发明的目的在于精确地控制微生物等的温度。 在本发明中，温度控制器包括用于储存微生物等的多个细胞，加热器和冷却部分。 加热器选择性地加热多个单元，并且冷却单元完全冷却多个单元。 当多个位置的最大温度值不低于第一下限时，驱动冷却部。 当一个地方的温度不高于第一个下限时，用于加热该地方的加热器被驱动。 当最大值不高于第二下限时，冷却部停止。 当一个地方的温度不低于第二个上限时，加热器的加热器不被驱动。

公开(公告)号：US20090236126A1

公开(公告)日：2009-09-24

申请号：US12227365

申请日：2007-06-06

申请人： Seiichiro Miyahara

发明人： Seiichiro Miyahara

IPC分类号： H05K1/02

CPC分类号： H05K1/025 ,  H05K1/028 ,  H05K2201/09263 ,  H05K2201/09281

摘要： A flexible wiring board is provided having a wiring structure which can reduce transmission loss by reducing impedance mismatching even if being folded in a three-dimensional manner. In a flexible wiring board 10 having a characteristic impedance control circuit 20, the flexible wiring board has a planar projection shape of a folded spot 20A in the characteristic impedance control circuit after folding in an arc state along a tangent.

摘要翻译： 提供了具有布线结构的柔性布线板，即使以三维方式折叠，也可以通过降低阻抗失配来降低传输损耗。 在具有特性阻抗控制电路20的柔性布线板10中，柔性布线板在沿着切线折叠成弧状态之后，在特性阻抗控制电路中具有折叠点20A的平面投影形状。

公开(公告)号：US20080280349A1

公开(公告)日：2008-11-13

申请号：US10585613

申请日：2004-07-30

申请人： Seiichiro Miyahara

发明人： Seiichiro Miyahara

IPC分类号： C12M1/38

CPC分类号： C12M41/12 ,  C12M41/48

摘要： The present invention has an object to selectively culture either one of mold and yeast, both of which are fungi, with priority. In order to attaint the object, a cell parts group 101 is heated by a heating mechanism 102 and cooled by a cooling mechanism 103. These operations are controlled by a heating-and-cooling control unit 104, and for instance, approximately 27° C. and 30 to 32° C. are switched to be adopted to a culturing temperature. Such temperature is set by a temperature-setting unit 105. By setting the culturing temperature to approximately 27° C. and 30 to 32° C., respectively, fungi and yeast will easily be selectively cultured with priority, respectively, in culture media provided in the cell parts group 101.

摘要翻译： 本发明的目的是优先选择性培养模具和酵母之一，这两者都是真菌。 为了达到上述目的，电池部件组101被加热机构102加热，并被冷却机构103冷却。 这些操作由加热和冷却控制单元104控制，并且例如约27℃和30至32℃被切换以用于培养温度。 这样的温度由温度设定单元105设定。通过将培养温度分别设定为约27℃和30〜32℃，真菌和酵母分别容易地选择性地培养在提供的培养基中 在细胞部分组101中。

公开(公告)号：US20080234874A1

公开(公告)日：2008-09-25

申请号：US11569772

申请日：2005-04-08

申请人： Seiichiro Miyahara

发明人： Seiichiro Miyahara

IPC分类号： G05D23/00

CPC分类号： G05D23/1934 ,  B01L3/50851 ,  B01L7/00 ,  C12M41/12 ,  C12M41/48

摘要： An object of the present invention is to accurately control temperatures of microorganisms or the like. In the present invention, a temperature controller comprises a plurality of cells for storing microorganisms or the like, heaters and a cooling section. The heaters selectively heat the plurality of cells, and the cooling section wholly cools the plurality of cells. When the maximum value of temperature of a plurality of places is not lower than a first lower limit, the cooling section is driven. When a temperature of one place is not higher than a first lower limit, the heater to heat the place is driven. When the maximum value is not higher than a second lower limit, the cooling section is stopped. When a temperature of one place is not lower than a second upper limit, the heater to heat the place is not driven.

摘要翻译： 本发明的目的在于精确地控制微生物等的温度。 在本发明中，温度控制器包括用于储存微生物等的多个细胞，加热器和冷却部分。 加热器选择性地加热多个单元，并且冷却单元完全冷却多个单元。 当多个位置的最大温度值不低于第一下限时，驱动冷却部。 当一个地方的温度不高于第一个下限时，用于加热该地方的加热器被驱动。 当最大值不高于第二下限时，冷却部停止。 当一个地方的温度不低于第二个上限时，加热器的加热器不被驱动。