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公开(公告)号：US20210317540A1
公开(公告)日：2021-10-14
申请号：US17356139
申请日：2021-06-23
申请人： Melissa May , Frederick H. Eggers , Kevin M. O'Brien , Peaches R. Ulrich , Benjamin A. Katchman , Shayla Freeman , Michael E. Hogan
发明人： Melissa May , Frederick H. Eggers , Kevin M. O'Brien , Peaches R. Ulrich , Benjamin A. Katchman , Shayla Freeman , Michael E. Hogan
IPC分类号： C12Q1/6895 , C12Q1/6806 , C12Q1/686 , C12Q1/6816 , G01N21/64
摘要： Provided herein is a method of quantitating a fungus in a plant, plant product or agricultural product. Total nucleic acids are isolated from a sample of the plant or plant product, and an asymmetric PCR amplification reaction is performed using fluorescent labeled primer pairs to obtain fluorescent labeled fungal amplicons. These amplicons are hybridized to fungus specific nucleic acid probes that are attached on a microarray support. The microarray is imaged to detect fluorescent signals from the fluorescent labeled fungal amplicons. The fluorescent signal intensity is correlated to the quantity of fungus.
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公开(公告)号：US10272409B2
公开(公告)日：2019-04-30
申请号：US12080720
申请日：2008-04-04
申请人： Michael E. Hogan , Sergy Lemeshko , Yuri Belosludtsev , Thomas F. Powdrill , Rahul Mitra , Joseph G. Utermohlen , Frederick H. Eggers
发明人： Michael E. Hogan , Sergy Lemeshko , Yuri Belosludtsev , Thomas F. Powdrill , Rahul Mitra , Joseph G. Utermohlen , Frederick H. Eggers
摘要： Provided herein are biomolecular hybridization devices comprising a substrate with a permanently and covalently attached surface of functional groups and an adsorbed monolayer of unmodified, single-stranded oligonucleotides all of which are 10 to about 24 bases in length as a saturated film of constrained oligonucleotides on the surface via direct non-covalent phosphate-surface adsorptive contact of substantially all phosphate groups of each oligonucleotide. The constrained oligonucleotides are effective to dissociably hybridize to a complementary single-stranded nucleic acid with asymmetric, non-helical base pairing and without oligonucleotide dissociation from the surface of the device. Also, provided are methods for hybridizing solution-state target nucleic acids to probe nucleic acids and for identifying a nucleotide sequence to which a nucleotide-binding protein binds using the biomolecular hybridization devices.
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公开(公告)号：US20120302457A1
公开(公告)日：2012-11-29
申请号：US13476774
申请日：2012-05-21
CPC分类号： C12Q1/6837 , C12Q1/6881 , C12Q2600/156
摘要： The present invention provides a portable system for real-time population-scale HLA genotyping and/or allelotyping in a field environment and methods of such population-scale HLA genotyping. The individual components of the system are portable to and operable within a field environment thereby providing high throughput with real-time geno- or allelotyping. Also provided are HLA gene-specific primers and HLA allele-specific or single nucleotide polymorphism-specific hybridization probes. In addition the present invention provides a microarray comprising the hybridization probes. Further provided is a kit comprising the HLA gene-specific primers and the microarray.
摘要翻译： 本发明提供了一种用于现场环境中实时种群规模HLA基因分型和/或等位基因的便携式系统以及这种群体规模HLA基因分型的方法。 系统的各个组件可以在现场环境中移植到并可操作，从而通过实时基因或等位基因提供高吞吐量。 还提供了HLA基因特异性引物和HLA等位基因特异性或单核苷酸多态性特异性杂交探针。 此外，本发明提供了包含杂交探针的微阵列。 还提供了包含HLA基因特异性引物和微阵列的试剂盒。
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公开(公告)号：US20210130898A1
公开(公告)日：2021-05-06
申请号：US17087099
申请日：2020-11-02
IPC分类号： C12Q1/6883 , G01N33/04 , C12N1/20 , C12N1/14
摘要： Provided herein is a method for identifying a mastitis-causing microbe in a subject. A milk sample is centrifuged to form a microbial pellet, total nucleic acids are extracted from the pellet and a microarray analysis of extracted DNA from which the mastitis-causing microbe is identified from DNA hybridization to mastitis-causing microbe species-specific gene probes. Also provided is a method for diagnosing a bovine mastitis infection in a dairy cow after identifying the bovine mastitis-causing microbe in a raw milk sample from the dairy cow.
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公开(公告)号：US07667026B2
公开(公告)日：2010-02-23
申请号：US11711561
申请日：2007-02-27
CPC分类号： C12Q1/6837 , C12Q1/6881 , C12Q2600/156
摘要： The present invention provides a portable system for real-time population-scale HLA genotyping and/or allelotyping in a field environment and methods of such population-scale HLA genotyping. The individual components of the system are portable to and operable within a field environment thereby providing high throughput with real-time geno- or allelotyping. Also provided are HLA gene-specific primers and HLA allele-specific or single nucleotide polymorphism-specific hybridization probes. In addition the present invention provides a microarray comprising the hybridization probes. Further provided is a kit comprising the HLA gene-specific primers and the microarray.
摘要翻译： 本发明提供了一种用于现场环境中实时种群规模HLA基因分型和/或等位基因的便携式系统以及这种群体规模HLA基因分型的方法。 系统的各个组件可以在现场环境中移植到并可操作，从而通过实时基因或等位基因提供高吞吐量。 还提供了HLA基因特异性引物和HLA等位基因特异性或单核苷酸多态性特异性杂交探针。 此外，本发明提供了包含杂交探针的微阵列。 还提供了包含HLA基因特异性引物和微阵列的试剂盒。
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公开(公告)号：US20220403476A1
公开(公告)日：2022-12-22
申请号：US17840835
申请日：2022-06-15
申请人： Fushi Wen , Frederick H. Eggers , Michael E. Hogan
发明人： Fushi Wen , Frederick H. Eggers , Michael E. Hogan
IPC分类号： C12Q1/70 , C12Q1/6837
摘要： Provided herein is a method for detecting the presence of a COVID-19 virus RNA or other pathogenic respiratory viruses, such as an influenza virus, or other RNA of interest in a sample. Nucleic acids are obtained from the sample and are used as a template in a combined isothermal reverse transcription, RNAse H and isothermal amplification reaction to generate single stranded RNA amplicons containing sequences complementary to fluorescent labeled detector probes. The single-stranded RNA amplicons hybridize to the detector probe and to hybridization probes with sequences complementary to a sequence determinant in the COVID-19 or other virus RNAs. The microarray is imaged to detect fluorescent signals thereby identifying the virus.
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公开(公告)号：US20140135231A1
公开(公告)日：2014-05-15
申请号：US14070819
申请日：2013-11-04
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , C12Q1/6881 , C12Q2600/156
摘要： The present invention provides a portable system for real-time population-scale HLA genotyping and/or allelotyping in a field environment and methods of such population-scale HLA genotyping. The individual components of the system are portable to and operable within a field environment thereby providing high throughput with real-time geno- or allelotyping. Also provided are HLA gene-specific primers and HLA allele-specific or single nucleotide polymorphism-specific hybridization probes. In addition the present invention provides a microarray comprising the hybridization probes. Further provided is a kit comprising the HLA gene-specific primers and the microarray.
摘要翻译： 本发明提供了一种用于现场环境中实时种群规模HLA基因分型和/或等位基因的便携式系统以及这种群体规模HLA基因分型的方法。 系统的各个组件可以在现场环境中移植到并可操作，从而通过实时基因或等位基因提供高吞吐量。 还提供了HLA基因特异性引物和HLA等位基因特异性或单核苷酸多态性特异性杂交探针。 此外，本发明提供了包含杂交探针的微阵列。 还提供了包含HLA基因特异性引物和微阵列的试剂盒。
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8.发明申请Methods and devices based upon a novel form of nucleic acid duplex on a surface 审中-公开基于表面上核酸双链体的新形式的方法和装置公开(公告)号：US20090011949A1
公开(公告)日：2009-01-08
申请号：US12080720
申请日：2008-04-04
申请人： Michael E. Hogan , Sergy Lemeshko , Yuri Belosludtsev , Thomas F. Powdrill , Rahul Mitra , Joseph G. Utermohlen , Frederick H. Eggers
发明人： Michael E. Hogan , Sergy Lemeshko , Yuri Belosludtsev , Thomas F. Powdrill , Rahul Mitra , Joseph G. Utermohlen , Frederick H. Eggers
CPC分类号： B01J19/0046 , B01J2219/00376 , B01J2219/00497 , B01J2219/005 , B01J2219/00576 , B01J2219/00605 , B01J2219/00653 , B01J2219/00659 , B01J2219/00677 , B01J2219/00691 , B01J2219/00722
摘要： Provided herein are biomolecular hybridization devices comprising a substrate with a permanently and covalently attached surface of functional groups and an adsorbed monolayer of unmodified, single-stranded oligonucleotides all of which are 10 to about 24 bases in length as a saturated film of constrained oligonucleotides on the surface via direct non-covalent phosphate-surface adsorptive contact of substantially all phosphate groups of each oligonucleotide. The constrained oligonucleotides are effective to dissociably hybridize to a complementary single-stranded nucleic acid with asymmetric, non-helical base pairing and without oligonucleotide dissociation from the surface of the device. Also, provided are methods for hybridizing solution-state target nucleic acids to probe nucleic acids and for identifying a nucleotide sequence to which a nucleotide-binding protein binds using the biomolecular hybridization devices.
摘要翻译： 本文提供的生物分子杂交装置包括具有永久且共价连接的官能团表面的底物和未修饰的单链寡核苷酸的吸附单层，其长度为10至约24个碱基，作为约束寡核苷酸的饱和膜 通过每个寡核苷酸的基本上所有磷酸基团的直接非共价磷酸盐表面吸附接触表面。 受约束的寡核苷酸有效地与具有不对称，非螺旋碱基配对并且没有寡核苷酸与装置表面解离的互补单链核酸解离地杂交。 此外，提供了用于将溶液状态靶核酸杂交以探测核酸并用于鉴定核苷酸结合蛋白使用生物分子杂交装置结合的核苷酸序列的方法。
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公开(公告)号：US20110117553A1
公开(公告)日：2011-05-19
申请号：US12924301
申请日：2010-09-24
申请人： Michael E. Hogan , Georgina Lopez Padilla , Melissa R. May , Andrew T. Abalos , Frederick H. Eggars , Kevin M. O'Brien
发明人： Michael E. Hogan , Georgina Lopez Padilla , Melissa R. May , Andrew T. Abalos , Frederick H. Eggars , Kevin M. O'Brien
CPC分类号： C12Q1/6881 , C12Q1/689 , C12Q2600/156
摘要： Provided are methods for amplifying a gene or RNA or sets thereof of interest using a tandem PCR process. The primers in the first PCR or set of PCR reactions are locus-specific. The primers in the second PCR or set of PCR reactions are specific for a sub-sequence of the locus-specific primers and completely consumed during the secondary PCR amplification. For RNA amplification, the first PCR is reverse transcription and the resulting cDNA(s) provide a template for cRNA synthesis, endpoint PCR or real time PCR. Also provided is a method of allelotyping a gene or set thereof by amplifying the gene(s) using tandem PCR on DNA or RNA comprising the sample, hybridizing the resulting amplicon or sets thereof to probes with sequences of gene-associated allele variations. A detectable signal indicating hybridization corresponds to an allelotype of the gene or a set of allelotypes for the set of genes.
摘要翻译： 提供了使用串联PCR方法扩增感兴趣的基因或RNA或其组的方法。 第一个PCR或一组PCR反应中的引物是基因座特异性的。 第二次PCR或一组PCR反应中的引物对基因座特异性引物的亚序列是特异性的，并且在二次PCR扩增期间完全消耗。 对于RNA扩增，第一个PCR是逆转录，所得到的cDNA提供了用于cRNA合成，终点PCR或实时PCR的模板。 还提供了通过使用串联PCR在包含样品的DNA或RNA上扩增基因来等位基因的方法，将得到的扩增子或其组合与具有基因相关等位基因变异序列的探针杂交。 指示杂交的可检测信号对应于基因的等位基因或该组基因的一组等位基因。
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公开(公告)号：US09416419B2
公开(公告)日：2016-08-16
申请号：US13317212
申请日：2011-10-12
申请人： Michael E. Hogan , Georgina Lopez Padilla , Melissa R. May , Andrew T. Abalos , Frederick H. Eggars , Kevin M. O'Brien
发明人： Michael E. Hogan , Georgina Lopez Padilla , Melissa R. May , Andrew T. Abalos , Frederick H. Eggars , Kevin M. O'Brien
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6881 , C12Q1/6804 , C12Q1/686 , C12Q1/689 , C12Q2600/156 , C12Q2600/16 , C12Q2527/143 , C12Q2563/107 , C12Q2565/501
摘要： Provided are methods for amplifying a gene or RNA or sets thereof of interest using a tandem PCR process. The primers in the first PCR or set of PCR reactions are locus-specific. The primers in the second PCR or set of PCR reactions are specific for a sub-sequence of the locus-specific primers and completely consumed during the second PCR amplification. For RNA amplification, the first PCR is reverse transcription and the resulting cDNA(s) provide a template for cRNA synthesis, endpoint PCR or real time PCR. Also provided is a tandem PCR method which accepts raw, completely unpurified mouthwash, cheek swabs and ORAGENE™-stabilized saliva as the sample input, the resulting amplicons serving as the substrate for complex, microarray-based genetic testing. Also provided is a method of allelotyping a gene or set thereof by amplifying the gene(s) using tandem PCR on DNA or RNA comprising the sample.
摘要翻译： 提供了使用串联PCR方法扩增感兴趣的基因或RNA或其组的方法。 第一个PCR或一组PCR反应中的引物是基因座特异性的。 第二次PCR或一组PCR反应中的引物对基因座特异性引物的亚序列是特异的，并且在第二次PCR扩增期间完全消耗。 对于RNA扩增，第一个PCR是逆转录，所得到的cDNA提供了用于cRNA合成，终点PCR或实时PCR的模板。 还提供了串联PCR方法，其接受原始的，完全未纯化的漱口水，颊拭子和ORAGENE TM稳定的唾液作为样品输入，所得扩增子用作复杂的基于微阵列的遗传测试的底物。 还提供了通过使用串联PCR在包含样品的DNA或RNA上扩增基因来等位基因的方法。
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