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公开(公告)号：US20120302457A1
公开(公告)日：2012-11-29
申请号：US13476774
申请日：2012-05-21
CPC分类号： C12Q1/6837 , C12Q1/6881 , C12Q2600/156
摘要： The present invention provides a portable system for real-time population-scale HLA genotyping and/or allelotyping in a field environment and methods of such population-scale HLA genotyping. The individual components of the system are portable to and operable within a field environment thereby providing high throughput with real-time geno- or allelotyping. Also provided are HLA gene-specific primers and HLA allele-specific or single nucleotide polymorphism-specific hybridization probes. In addition the present invention provides a microarray comprising the hybridization probes. Further provided is a kit comprising the HLA gene-specific primers and the microarray.
摘要翻译： 本发明提供了一种用于现场环境中实时种群规模HLA基因分型和/或等位基因的便携式系统以及这种群体规模HLA基因分型的方法。 系统的各个组件可以在现场环境中移植到并可操作，从而通过实时基因或等位基因提供高吞吐量。 还提供了HLA基因特异性引物和HLA等位基因特异性或单核苷酸多态性特异性杂交探针。 此外，本发明提供了包含杂交探针的微阵列。 还提供了包含HLA基因特异性引物和微阵列的试剂盒。
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公开(公告)号：US20140135231A1
公开(公告)日：2014-05-15
申请号：US14070819
申请日：2013-11-04
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , C12Q1/6881 , C12Q2600/156
摘要： The present invention provides a portable system for real-time population-scale HLA genotyping and/or allelotyping in a field environment and methods of such population-scale HLA genotyping. The individual components of the system are portable to and operable within a field environment thereby providing high throughput with real-time geno- or allelotyping. Also provided are HLA gene-specific primers and HLA allele-specific or single nucleotide polymorphism-specific hybridization probes. In addition the present invention provides a microarray comprising the hybridization probes. Further provided is a kit comprising the HLA gene-specific primers and the microarray.
摘要翻译： 本发明提供了一种用于现场环境中实时种群规模HLA基因分型和/或等位基因的便携式系统以及这种群体规模HLA基因分型的方法。 系统的各个组件可以在现场环境中移植到并可操作，从而通过实时基因或等位基因提供高吞吐量。 还提供了HLA基因特异性引物和HLA等位基因特异性或单核苷酸多态性特异性杂交探针。 此外，本发明提供了包含杂交探针的微阵列。 还提供了包含HLA基因特异性引物和微阵列的试剂盒。
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公开(公告)号：US07667026B2
公开(公告)日：2010-02-23
申请号：US11711561
申请日：2007-02-27
CPC分类号： C12Q1/6837 , C12Q1/6881 , C12Q2600/156
摘要： The present invention provides a portable system for real-time population-scale HLA genotyping and/or allelotyping in a field environment and methods of such population-scale HLA genotyping. The individual components of the system are portable to and operable within a field environment thereby providing high throughput with real-time geno- or allelotyping. Also provided are HLA gene-specific primers and HLA allele-specific or single nucleotide polymorphism-specific hybridization probes. In addition the present invention provides a microarray comprising the hybridization probes. Further provided is a kit comprising the HLA gene-specific primers and the microarray.
摘要翻译： 本发明提供了一种用于现场环境中实时种群规模HLA基因分型和/或等位基因的便携式系统以及这种群体规模HLA基因分型的方法。 系统的各个组件可以在现场环境中移植到并可操作，从而通过实时基因或等位基因提供高吞吐量。 还提供了HLA基因特异性引物和HLA等位基因特异性或单核苷酸多态性特异性杂交探针。 此外，本发明提供了包含杂交探针的微阵列。 还提供了包含HLA基因特异性引物和微阵列的试剂盒。
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公开(公告)号：US10272409B2
公开(公告)日：2019-04-30
申请号：US12080720
申请日：2008-04-04
申请人： Michael E. Hogan , Sergy Lemeshko , Yuri Belosludtsev , Thomas F. Powdrill , Rahul Mitra , Joseph G. Utermohlen , Frederick H. Eggers
发明人： Michael E. Hogan , Sergy Lemeshko , Yuri Belosludtsev , Thomas F. Powdrill , Rahul Mitra , Joseph G. Utermohlen , Frederick H. Eggers
摘要： Provided herein are biomolecular hybridization devices comprising a substrate with a permanently and covalently attached surface of functional groups and an adsorbed monolayer of unmodified, single-stranded oligonucleotides all of which are 10 to about 24 bases in length as a saturated film of constrained oligonucleotides on the surface via direct non-covalent phosphate-surface adsorptive contact of substantially all phosphate groups of each oligonucleotide. The constrained oligonucleotides are effective to dissociably hybridize to a complementary single-stranded nucleic acid with asymmetric, non-helical base pairing and without oligonucleotide dissociation from the surface of the device. Also, provided are methods for hybridizing solution-state target nucleic acids to probe nucleic acids and for identifying a nucleotide sequence to which a nucleotide-binding protein binds using the biomolecular hybridization devices.
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5.发明申请Methods and devices based upon a novel form of nucleic acid duplex on a surface 审中-公开基于表面上核酸双链体的新形式的方法和装置公开(公告)号：US20090011949A1
公开(公告)日：2009-01-08
申请号：US12080720
申请日：2008-04-04
申请人： Michael E. Hogan , Sergy Lemeshko , Yuri Belosludtsev , Thomas F. Powdrill , Rahul Mitra , Joseph G. Utermohlen , Frederick H. Eggers
发明人： Michael E. Hogan , Sergy Lemeshko , Yuri Belosludtsev , Thomas F. Powdrill , Rahul Mitra , Joseph G. Utermohlen , Frederick H. Eggers
CPC分类号： B01J19/0046 , B01J2219/00376 , B01J2219/00497 , B01J2219/005 , B01J2219/00576 , B01J2219/00605 , B01J2219/00653 , B01J2219/00659 , B01J2219/00677 , B01J2219/00691 , B01J2219/00722
摘要： Provided herein are biomolecular hybridization devices comprising a substrate with a permanently and covalently attached surface of functional groups and an adsorbed monolayer of unmodified, single-stranded oligonucleotides all of which are 10 to about 24 bases in length as a saturated film of constrained oligonucleotides on the surface via direct non-covalent phosphate-surface adsorptive contact of substantially all phosphate groups of each oligonucleotide. The constrained oligonucleotides are effective to dissociably hybridize to a complementary single-stranded nucleic acid with asymmetric, non-helical base pairing and without oligonucleotide dissociation from the surface of the device. Also, provided are methods for hybridizing solution-state target nucleic acids to probe nucleic acids and for identifying a nucleotide sequence to which a nucleotide-binding protein binds using the biomolecular hybridization devices.
摘要翻译： 本文提供的生物分子杂交装置包括具有永久且共价连接的官能团表面的底物和未修饰的单链寡核苷酸的吸附单层，其长度为10至约24个碱基，作为约束寡核苷酸的饱和膜 通过每个寡核苷酸的基本上所有磷酸基团的直接非共价磷酸盐表面吸附接触表面。 受约束的寡核苷酸有效地与具有不对称，非螺旋碱基配对并且没有寡核苷酸与装置表面解离的互补单链核酸解离地杂交。 此外，提供了用于将溶液状态靶核酸杂交以探测核酸并用于鉴定核苷酸结合蛋白使用生物分子杂交装置结合的核苷酸序列的方法。
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公开(公告)号：US20210130898A1
公开(公告)日：2021-05-06
申请号：US17087099
申请日：2020-11-02
IPC分类号： C12Q1/6883 , G01N33/04 , C12N1/20 , C12N1/14
摘要： Provided herein is a method for identifying a mastitis-causing microbe in a subject. A milk sample is centrifuged to form a microbial pellet, total nucleic acids are extracted from the pellet and a microarray analysis of extracted DNA from which the mastitis-causing microbe is identified from DNA hybridization to mastitis-causing microbe species-specific gene probes. Also provided is a method for diagnosing a bovine mastitis infection in a dairy cow after identifying the bovine mastitis-causing microbe in a raw milk sample from the dairy cow.
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公开(公告)号：US20220403476A1
公开(公告)日：2022-12-22
申请号：US17840835
申请日：2022-06-15
申请人： Fushi Wen , Frederick H. Eggers , Michael E. Hogan
发明人： Fushi Wen , Frederick H. Eggers , Michael E. Hogan
IPC分类号： C12Q1/70 , C12Q1/6837
摘要： Provided herein is a method for detecting the presence of a COVID-19 virus RNA or other pathogenic respiratory viruses, such as an influenza virus, or other RNA of interest in a sample. Nucleic acids are obtained from the sample and are used as a template in a combined isothermal reverse transcription, RNAse H and isothermal amplification reaction to generate single stranded RNA amplicons containing sequences complementary to fluorescent labeled detector probes. The single-stranded RNA amplicons hybridize to the detector probe and to hybridization probes with sequences complementary to a sequence determinant in the COVID-19 or other virus RNAs. The microarray is imaged to detect fluorescent signals thereby identifying the virus.
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公开(公告)号：US20210317540A1
公开(公告)日：2021-10-14
申请号：US17356139
申请日：2021-06-23
申请人： Melissa May , Frederick H. Eggers , Kevin M. O'Brien , Peaches R. Ulrich , Benjamin A. Katchman , Shayla Freeman , Michael E. Hogan
发明人： Melissa May , Frederick H. Eggers , Kevin M. O'Brien , Peaches R. Ulrich , Benjamin A. Katchman , Shayla Freeman , Michael E. Hogan
IPC分类号： C12Q1/6895 , C12Q1/6806 , C12Q1/686 , C12Q1/6816 , G01N21/64
摘要： Provided herein is a method of quantitating a fungus in a plant, plant product or agricultural product. Total nucleic acids are isolated from a sample of the plant or plant product, and an asymmetric PCR amplification reaction is performed using fluorescent labeled primer pairs to obtain fluorescent labeled fungal amplicons. These amplicons are hybridized to fungus specific nucleic acid probes that are attached on a microarray support. The microarray is imaged to detect fluorescent signals from the fluorescent labeled fungal amplicons. The fluorescent signal intensity is correlated to the quantity of fungus.
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公开(公告)号：US12054794B2
公开(公告)日：2024-08-06
申请号：US17840835
申请日：2022-06-15
申请人： Fushi Wen , Frederick H. Eggers , Michael E. Hogan
发明人： Fushi Wen , Frederick H. Eggers , Michael E. Hogan
IPC分类号： C12Q1/70 , C12Q1/6818 , C12Q1/6837 , C12Q1/6853 , C12Q1/689
CPC分类号： C12Q1/701 , C12Q1/6837 , C12Q1/6818 , C12Q1/6853 , C12Q1/689 , C12Q2600/16
摘要： Provided herein is a method for detecting the presence of a COVID-19 virus RNA or other pathogenic respiratory viruses, such as an influenza virus, or other RNA of interest in a sample. Nucleic acids are obtained from the sample and are used as a template in a combined isothermal reverse transcription, RNAse H and isothermal amplification reaction to generate single stranded RNA amplicons containing sequences complementary to fluorescent labeled detector probes. The single-stranded RNA amplicons hybridize to the detector probe and to hybridization probes with sequences complementary to a sequence determinant in the COVID-19 or other virus RNAs. The microarray is imaged to detect fluorescent signals thereby identifying the virus.
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公开(公告)号：US5614503A
公开(公告)日：1997-03-25
申请号：US467114
申请日：1995-06-06
IPC分类号： A61K9/127 , A61K48/00 , C07J41/00 , C07C261/00 , C07J9/00
CPC分类号： C07J41/0055 , A61K48/00 , A61K9/1272
摘要： A nucleic acid transporter to deliver a nucleic acids into cells, comprising a cationic compound having a cationic head group for binding the nucleic acid and a lipid tail for association with the membrane. A cationic compound usually is a polyamine or a short basic peptide. The lipid tail is usually selected from the group consisting of plant steroid, animal steroid, isoprenoid compound, aliphatic lipid, pore forming protein, pore forming peptides and fusogenic peptides. The cationic head and the lipid tail are linked through a carbamate linkage. When polyamine is used, it is preferably either spermidine or spermine and the nucleic acid can be any of a variety, including triplex forming oligonucleotides, antisense oligonucleotide, aptamers, ribozymes, plasmids and DNA for gene therapy. Also described is a method for treating individuals using the transporter linked to a therapeutic nucleic acid.
摘要翻译： 将核酸递送到细胞中的核酸转运蛋白，其包含具有用于结合核酸的阳离子头基的阳离子化合物和与所述膜缔合的脂质尾。 阳离子化合物通常是多胺或短碱性肽。 脂质尾巴通常选自植物类固醇，动物类固醇，类异戊二烯化合物，脂肪族脂质，成孔蛋白，成孔肽和融合肽。 阳离子头和脂质尾巴通过氨基甲酸酯键连接。 当使用多胺时，其优选为亚精胺或精胺，核酸可为任何多种，包括形成三链体的寡核苷酸，反义寡核苷酸，适体，核酶，质粒和用于基因治疗的DNA。 还描述了使用与治疗性核酸连接的转运蛋白来治疗个体的方法。
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