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公开(公告)号：US07892817B2
公开(公告)日：2011-02-22
申请号：US11569084
申请日：2005-05-13
申请人： Menno Willem Jose Prins , Martinus Barnardus Van Der Mark , Paulus Cornelis Duineveld , Mischa Megens , Josephus Arnoldus Henricus Maria Kahlman
发明人： Menno Willem Jose Prins , Martinus Barnardus Van Der Mark , Paulus Cornelis Duineveld , Mischa Megens , Josephus Arnoldus Henricus Maria Kahlman
IPC分类号： C12M3/00
CPC分类号： G01N33/54373 , B82Y5/00 , B82Y25/00 , G01N27/745
摘要： A method and a device are provided for distinguishing a specific binding from a less specific binding between at least one magnetic nanoparticle and a surface of another entity by applying a magnetic field and detecting a physical parameter relating to magnetic nanoparticle rotational or motional freedom while the magnetic nanoparticle is attached to the surface. The method and device may be applied to in-vivo and in-vitro biomolecular diagnostics. The sensor combines in one sensor the detection of magnetic particles or labels and determination of the binding quality and the properties of magnetic particles or labels which are bound to the surface of another entity.
摘要翻译： 提供了一种方法和装置，用于通过施加磁场和检测与磁性纳米颗粒旋转或运动自由相关的物理参数来区分特定结合与至少一个磁性纳米颗粒与另一个实体的表面之间的较小特异性结合，同时磁性 纳米颗粒附着在表面。 该方法和装置可以应用于体内和体外生物分子诊断。 该传感器将一个传感器结合在一起，检测磁性颗粒或标签，并确定结合质量以及结合到另一个实体表面的磁性颗粒或标签的性质。
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公开(公告)号：US20080191688A1
公开(公告)日：2008-08-14
申请号：US11814826
申请日：2006-01-30
申请人： Josephus Arnoldus Henricus Maria Kahlman , Rachel Estelle Thilwind , Menno Willem Jose Prins , Mischa Megens , Reinhold Wimberger-Friedl
发明人： Josephus Arnoldus Henricus Maria Kahlman , Rachel Estelle Thilwind , Menno Willem Jose Prins , Mischa Megens , Reinhold Wimberger-Friedl
IPC分类号： G01N33/543
CPC分类号： G01N27/745 , G01N33/54333 , G01N33/553
摘要： A sensor device (15) for detecting magnetic particles (13) has a binding surface (40) with binding sites thereon and comprises: at least one sensor element (23) for detecting the presence of magnetic particles (13), means for attracting magnetic structures comprising at least one magnetic particle (13) toward and onto the binding surface (40) of the sensor device (15), and means for re-arranging and randomizing the position of individual magnetic particles (13) with respect to the binding sites on the binding surface (40) to give binding sites on all individual particles (13) a substantial probability to have a contact time with binding sites on the binding surface (40). With such sensor device (15), the speed of detection of target molecules in a fluid is enhanced.
摘要翻译： 用于检测磁性颗粒（13）的传感器装置（15）具有在其上具有结合位置的结合表面（40），并包括：用于检测磁性颗粒（13）的存在的至少一个传感器元件（23），用于吸引磁性颗粒 包括朝向和传递到传感器装置（15）的结合表面（40）上的至少一个磁性颗粒（13）的结构，以及用于重新排列和随机化各个磁性颗粒（13）相对于结合位点 在结合表面（40）上以在所有单独的颗粒（13）上产生与结合表面（40）上的结合位点具有接触时间的可能性。 利用这种传感器装置（15），提高了流体中目标分子的检测速度。
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公开(公告)号：US08828740B2
公开(公告)日：2014-09-09
申请号：US11814826
申请日：2006-01-30
申请人： Josephus Arnoldus Henricus Maria Kahlman , Rachel Estelle Thilwind , Menno Willem Jose Prins , Mischa Megens , Reinhold Wimberger-Friedl
发明人： Josephus Arnoldus Henricus Maria Kahlman , Rachel Estelle Thilwind , Menno Willem Jose Prins , Mischa Megens , Reinhold Wimberger-Friedl
IPC分类号： G01N33/553 , G01N27/74 , G01N33/543
CPC分类号： G01N27/745 , G01N33/54333 , G01N33/553
摘要： A sensor device (15) for detecting magnetic particles (13) has a binding surface (40) with binding sites thereon and includes at least one sensor element (23) for detecting the presence of magnetic particles (13), an element or elements for attracting magnetic structures having at least one magnetic particle (13) toward and onto the binding surface (40) of the sensor device (15), and an element or elements for re-arranging and randomizing the position of individual magnetic particles (13) with respect to the binding sites on the binding surface (40) to give binding sites on all individual particles (13) a substantial probability to have a contact time with binding sites on the binding surface (40). With such sensor device (15), the speed of detection of target molecules in a fluid is enhanced.
摘要翻译： 用于检测磁性颗粒（13）的传感器装置（15）具有在其上具有结合位置的结合表面（40），并且包括用于检测磁性颗粒（13）的存在的至少一个传感器元件（23），用于 吸引具有至少一个磁性颗粒（13）的磁性结构朝向和传递到传感器装置（15）的结合表面（40）上，以及用于重新排列和随机化各个磁性颗粒（13）的位置的元件或元件， 相对于结合表面（40）上的结合位点以在所有单个颗粒（13）上产生与结合表面（40）上的结合位点具有接触时间的可能性。 利用这种传感器装置（15），提高了流体中目标分子的检测速度。
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公开(公告)号：US20090206832A1
公开(公告)日：2009-08-20
申请号：US12307790
申请日：2007-07-02
CPC分类号： G01R33/093 , B82Y25/00 , G01N27/745 , G01N33/54333 , G01R33/12 , G01R33/1269
摘要： The present invention provides a magnetic sensor device comprising means (11, 14) for increasing a binding possibility of magnetic or magnetizable objects (15) e.g. magnetic particles, onto the binding sites by applying a moving magnetic field. The present invention furthermore provides a biochip (40) comprising at least one such magnetic sensor and a method for detecting and/or quantifying target moieties in a sample fluid by using such a magnetic sensor.
摘要翻译： 本发明提供了一种磁传感器装置，其包括用于增加磁性或可磁化物体（15）的结合可能性的装置（11,14），例如， 磁性颗粒，通过施加移动磁场到结合位点。 本发明还提供了一种生物芯片（40），其包括至少一个这样的磁传感器以及通过使用这种磁传感器来检测和/或定量样品流体中的目标部分的方法。
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5.发明申请ATTRACTION AND REPULSION OF MAGNETIC OF MAGNETIZABLE OBJECTS TO AND FROM A SENSOR SURFACE 审中-公开磁传感器和磁传感器表面的磁化吸收和释放公开(公告)号：US20090251136A1
公开(公告)日：2009-10-08
申请号：US12373740
申请日：2007-06-06
CPC分类号： G01N15/0656 , B82Y25/00 , G01N27/745 , G01R33/093 , G01R33/12 , G01R33/1269
摘要： The present invention provides a magnetic sensor device a first magnetic field generating means (21a, 21b) for attracting magnetic or magnetizable objects (22), e.g. magnetic particles, to a sensor surface (23) and a second magnetic field generating means (25) for, in combination with the first magnetic field, repelling magnetic or magnetizable objects (22), e.g. magnetic particles, from the sensor surface (23). The magnetic fields generated by the first and second magnetic field generating means have substantially anti-parallel directions. The present invention furthermore provides a method for attracting and repelling magnetic or magnetizable objects (22), e.g. magnetic particles, to and from a sensor surface (23).
摘要翻译： 本发明提供一种磁传感器装置，用于吸引磁性或可磁化物体（22）的第一磁场产生装置（21a，21b） 传感器表面（23）和第二磁场产生装置（25），用于与第一磁场结合，排斥磁性或可磁化物体（22），例如磁性颗粒。 磁性颗粒，来自传感器表面（23）。 由第一和第二磁场产生装置产生的磁场具有大致反平行的方向。 本发明还提供了一种用于吸引和排斥磁性或可磁化物体（22）的方法，例如， 磁性颗粒，传送到传感器表面（23）。
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公开(公告)号：US20080309329A1
公开(公告)日：2008-12-18
申请号：US10597639
申请日：2004-07-29
IPC分类号： G01R33/12
CPC分类号： G01R33/12 , G01N27/745 , G01R33/1269
摘要： A magnetic sensor device is disclosed for the detection or determination of a magnetic field, such as for example, but not limited to, the presence of magnetic particles (15). In particular it relates to an integrated or on-chip magnetic sensor element (11) for the detection of magnetic nano-particles functioning as a label to biological molecules. The device of the present invention offers reduced cross-talk between the magnetic sensor element (11) and a magnetic field generator (12) by compensating capacitive and/or magnetic coupling for instance by using an electrostatic shield and/or modulation and demodulation techniques. The device may be used for magnetic detection of binding of biological molecules on a micro-array or biochip.
摘要翻译： 公开了用于检测或确定磁场的磁传感器装置，例如但不限于存在磁性颗粒（15）。 特别地，其涉及用于检测用作生物分子的标记的磁性纳米颗粒的集成或片上磁传感器元件（11）。 本发明的装置通过例如通过使用静电屏蔽和/或调制和解调技术来补偿电容和/或磁耦合来减小磁传感器元件（11）和磁场发生器（12）之间的串扰。 该装置可用于磁性检测微阵列或生物芯片上生物分子的结合。
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公开(公告)号：US20090186420A1
公开(公告)日：2009-07-23
申请号：US12302903
申请日：2007-05-22
CPC分类号： G01N27/745 , G01N35/0098
摘要： The invention relates to a microelectronic sensor device, particularly to a bio sensor with magnetic sensor units comprising excitation wires (11, 13) and a GMR sensor (12). The device further comprises a washing unit (20) consisting of a series of actuation wires (21) coupled to a driving unit (22). The driving unit (22) can activate the actuation wires selectively, wherein magnetic washing particles (2) are attracted to the activated wires. Shifting the activation pattern (R, S, T) then results in a corresponding movement of washing particles (2), which induces flow of the sample fluid that can wash away weakly bound and/or unbound target substance (3) from the sensor region (10) of the sensor units.
摘要翻译： 本发明涉及一种微电子传感器装置，特别涉及具有包括激励线（11,13）和GMR传感器（12）的磁传感器单元的生物传感器。 该装置还包括由连接到驱动单元（22）的一系列致动线（21）组成的洗涤单元（20）。 驱动单元（22）可以选择性地启动致动线，其中磁性洗涤颗粒（2）被吸引到活化的丝线上。 移动激活模式（R，S，T）然后导致洗涤颗粒（2）的相应移动，其引起样品流体的流动，其可以从传感器区域洗脱弱结合和/或未结合的目标物质（3） （10）的传感器单元。
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公开(公告)号：US20090105087A1
公开(公告)日：2009-04-23
申请号：US12282713
申请日：2007-03-05
CPC分类号： B01L3/502738 , B01L3/502761 , B01L2200/0647 , B01L2200/148 , B01L2200/16 , B01L2300/0816 , B01L2400/0415 , B01L2400/0683 , G01N35/10 , G01N35/1002 , G01N35/1016 , G01N2035/1034
摘要： The invention relates to microelectronic device (100) and a method that allow the controlled release of a reference substance (21, 22, 23) into the sample chamber (5) of a biosensor. In a particular embodiment, this is achieved by a supply unit (20) comprising a control wire (24) that can generate a magnetic field in a storage region of the sample chamber (5). Magnetic reference particles (22) can then enclose and retain reference target molecules (21) until the magnetic field is reduced or switched off, releasing the target molecules at a desired point in time. The controlled release of a reference substance (21, 22, 23) may for instance be used for the calibration of a magnetic biosensor.
摘要翻译： 本发明涉及微电子器件（100）和允许参考物质（21,22,23）被控制释放到生物传感器的样品室（5）中的方法。 在一个具体实施例中，这是通过一个供应单元（20）实现的，该供应单元包括可以在样品室（5）的存储区域中产生磁场的控制线（24）。 磁参考颗粒（22）然后可以包围并保留参考目标分子（21），直到磁场减少或切断，在期望的时间点释放靶分子。 参考物质（21,22,23）的控制释放可以例如用于校准磁性生物传感器。
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公开(公告)号：US20090072815A1
公开(公告)日：2009-03-19
申请号：US12298066
申请日：2007-04-16
CPC分类号： G01V13/00 , B82Y25/00 , G01R33/09 , G01R33/093 , G01R33/12 , G01R33/1269
摘要： The invention relates to the calibration of the magnetic sensor device comprising magnetic excitation wires (11, 13) and a magnetic sensor element, for example a GMR sensor (12), for measuring reaction fields (B2) generated by magnetic particles (2) in reaction to an excitation field (B1) generated by the excitation wires. The magnetic sensor element (12) can be calibrated by saturating the magnetic particles (2) with a magnetic calibration field (B3). Thus the direct (crosstalk) action of the excitation field (B1) on the magnetic sensor element (12) can be determined without disturbing contributions of the magnetic particles (2).
摘要翻译： 本发明涉及包括磁激励线（11,13）和磁传感器元件例如GMR传感器（12）的磁传感器装置的校准，用于测量由磁性颗粒（2）产生的反应场（B2） 对由激励线产生的激发场（B1）的反应。 磁传感器元件（12）可通过用磁性校准场（B3）使磁性颗粒（2）饱和来校准。 因此，可以确定励磁场（B1）对磁性传感器元件（12）的直接（串扰）作用，而不会影响磁性颗粒（2）的贡献。
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公开(公告)号：US08486689B2
公开(公告)日：2013-07-16
申请号：US12747529
申请日：2008-10-31
申请人： Dominique Maria Bruls , Johannes Joseph Hubertina Barbara Schleipen , Josephus Arnoldus Henricus Maria Kahlman , Menno Willem Jose Prins
发明人： Dominique Maria Bruls , Johannes Joseph Hubertina Barbara Schleipen , Josephus Arnoldus Henricus Maria Kahlman , Menno Willem Jose Prins
IPC分类号： C12M1/00
CPC分类号： G01N21/1717 , G01N21/552 , G01N21/648 , G01N2021/1721 , G01N2021/1727 , G01N2201/0691
摘要： The invention relates to a microelectronic sensor device for the examination of target particles (1) that are bound to binding sites (3) at the binding surface (12) of a carrier (11). In a preferred embodiment, an input light beam (L1) is transmitted into the carrier (11), where a frustrated total internal reflection (FTIR) takes place at the binding surface (12). The amount of light in a resulting output light beam (L2) is detected by a light detector (31) and provides information about the presence of target particles at the binding surface. Moreover, an actuation unit (50) induces movements of the bound target particles (1) by an interaction with a magnetic field (B) or an electric field, particularly with a given modulation frequency (COIn), such that by a demodulation of the detector signal (S) effects of the target particles can be distinguished from background.
摘要翻译： 本发明涉及一种用于检查在载体（11）的结合表面（12）处结合到结合位点（3）的靶颗粒（1）的微电子传感器装置。 在优选实施例中，输入光束（L1）被传输到载体（11）中，其中在结合表面（12）处发生挫败的全内反射（FTIR）。 通过光检测器（31）检测所得到的输出光束（L2）中的光量，并提供关于在结合表面处存在目标颗粒的信息。 此外，致动单元（50）通过与磁场（B）或电场的相互作用（特别是给定的调制频率（COIn））的相互作用来诱导结合的目标颗粒（1）的运动，使得通过解调 目标颗粒的检测器信号（S）效应可以与背景区分开。
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