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    Process for purifying a compound
    4.
    发明授权
    Process for purifying a compound 失效
    纯化化合物的方法

    公开(公告)号：US07193056B2

    公开(公告)日：2007-03-20

    申请号：US10863456

    申请日：2004-06-08

    申请人： Hans Ageland ,  Lena Nyström ,  Josefine Persson ,  Folke Tjerneld

    发明人： Hans Ageland ,  Lena Nyström ,  Josefine Persson ,  Folke Tjerneld

    IPC分类号： C07K1/14

    CPC分类号： C07K14/775 ,  A61K38/00

    摘要： The present invention relates to a process for purifying a hydrophobic or amphiphilic compound, by first mixing a starting material containing the hydrophobic or amphiphilic compound with a first polymeric material, water and at least one of a second polymeric material and a surfactant, wherein the first polymeric material and the second polymeric material and/or surfactant are immiscible in the resulting primary aqueous solution. The process further comprises maintaining the primary aqueous solution for a period of time sufficient for essentially separating the phases formed, and then removing the phase containing the main portion of the hydrophobic or amphiphilic compound and the second polymeric material and/or surfactant. The second polymeric material and/or surfactant are separated from the hydrophobic or amphiphilic compound, and subsequently recycled to the initial mixing step. The present invention further relates to a composition for use in purification of apolipoprotein A (ApoA) or apolipoprotein E (ApoE), said composition comprising a first polymeric material and a surfactant, said first polymeric material and surfactant being immiscible in the primary aqueous solution obtained after mixing with water. ApoA or ApoE produced by the inventive process can be used for the manufacture of a medicament in the treatment of atherosclerosis and cardiovascular diseases, sepsis or peripheral atherosclerosis as well as in a method for treatment of atherosclerosis and cardiovascular diseases, sepsis or peripheral atherosclerosis when administered in a therapeutically effective amount.

    摘要翻译： 本发明涉及一种纯化疏水性或两亲性化合物的方法，首先将含有疏水性或两亲性化合物的原料与第一聚合物材料，水和至少一种第二聚合物材料和表面活性剂混合，其中第一 聚合物材料和第二聚合物材料和/或表面活性剂在所得初级水溶液中是不混溶的。 该方法还包括将初级水溶液保持一段时间，足以基本分离所形成的相，然后除去含有疏水性或两亲性化合物和第二聚合物材料和/或表面活性剂的主要部分的相。 将第二聚合物材料和/或表面活性剂与疏水性或两亲性化合物分离，随后循环至初始混合步骤。 本发明还涉及用于纯化载脂蛋白A（ApoA）或载脂蛋白E（ApoE）的组合物，所述组合物包含第一聚合物材料和表面活性剂，所述第一聚合物材料和表面活性剂在获得的主要水溶液中是不混溶的 与水混合后。 本发明方法产生的ApoA或ApoE可用于制备治疗动脉粥样硬化和心血管疾病，败血症或外周动脉粥样硬化的药物，以及用于治疗动脉粥样硬化和心血管疾病，败血症或外周动脉粥样硬化的方法 治疗有效量。

    Process for purifying a compound
    5.
    发明授权
    Process for purifying a compound 失效
    纯化化合物的方法

    公开(公告)号：US06767994B1

    公开(公告)日：2004-07-27

    申请号：US09571683

    申请日：2000-05-16

    申请人： Hans Ageland ,  Lena Nyström ,  Josefine Persson ,  Folke Tjerneld

    发明人： Hans Ageland ,  Lena Nyström ,  Josefine Persson ,  Folke Tjerneld

    IPC分类号： C07K114

    CPC分类号： C07K14/775 ,  A61K38/00

    摘要： The present invention relates to a process for purifying a hydrophobic or amphiphilic compound, by first mixing a starting material containing the hydrophobic or amphiphilic compound with a first polymeric material, water and at least one of a second polymeric material and a surfactant, wherein the first polymeric material and the second polymeric material and/or surfactant are immiscible in the resulting primary aqueous solution. The process further comprises maintaining the primary aqueous solution for a period of time sufficient for essentially separating the phases formed, and then removing the phase containing the main portion of the hydrophobic or amphiphilic compound and the second polymeric material and/or surfactant. The second polymeric material and/or surfactant are separated from the hydrophobic or amphiphilic compound, and subsequently recycled to the initial mixing step. The present invention further relates to a composition for use in purification of apolipoprotein A (ApoA) or apolipoprotein E (ApoE), said composition comprising a first polymeric material and a surfactant, said first polymeric material and surfactant being immiscible in the primary aqueous solution obtained after mixing with water. ApoA or ApoE produced by the inventive process can be used for the manufacture of a medicament in the treatment of atherosclerosis and cardiovascular diseases, sepsis or peripheral atherosclerosis as well as in a method for treatment of atherosclerosis and cardiovascular diseases, sepsis or peripheral atherosclerosis when administered in a therapeutically effective amount.

    摘要翻译： 本发明涉及一种纯化疏水性或两亲性化合物的方法，首先将含有疏水性或两亲性化合物的原料与第一聚合物材料，水和至少一种第二聚合物材料和表面活性剂混合，其中第一 聚合物材料和第二聚合物材料和/或表面活性剂在所得初级水溶液中是不混溶的。 该方法还包括将初级水溶液保持一段时间，足以基本分离所形成的相，然后除去含有疏水性或两亲性化合物和第二聚合物材料和/或表面活性剂的主要部分的相。 将第二聚合物材料和/或表面活性剂与疏水性或两亲性化合物分离，随后循环至初始混合步骤。 本发明还涉及用于纯化载脂蛋白A（ApoA）或载脂蛋白E（ApoE）的组合物，所述组合物包含第一聚合物材料和表面活性剂，所述第一聚合物材料和表面活性剂在获得的主要水溶液中是不混溶的 与水混合后。 本发明方法产生的ApoA或ApoE可用于制备治疗动脉粥样硬化和心血管疾病，败血症或外周动脉粥样硬化的药物，以及用于治疗动脉粥样硬化和心血管疾病，败血症或外周动脉粥样硬化的方法 治疗有效量。

    Process for purifying a protein
    6.
    发明授权
    Process for purifying a protein 失效
    纯化蛋白质的方法

    公开(公告)号：US06559284B1

    公开(公告)日：2003-05-06

    申请号：US08924994

    申请日：1997-09-05

    申请人： Hans Ageland ,  Lena Nyström ,  Josefine Persson ,  Folke Tjerneld

    发明人： Hans Ageland ,  Lena Nyström ,  Josefine Persson ,  Folke Tjerneld

    IPC分类号： C07K114

    CPC分类号： C07K14/775

    摘要： The present invention relates to a composition for use in purification of apolipoprotein A (ApoA) or apolipoprotein E (ApoE), said composition comprising a first and a second polymeric material, wherein the first and second polymeric material are immiscible in the primary aqueous solution, and wherein the second polymeric material is amphiphilic and water soluble. The invention further relates to a process for purifying ApoA or ApoE, or variants or mixtures thereof, by first mixing ApoA or ApoE, the composition containing a first and second polymeric material and water. The resulting primary aqueous solution is maintained for a period of time sufficient for essentially separating the phases formed, and removing the phase containing the second polymeric material and the main portion of ApoA or ApoE. Subsequently, the second polymeric material is separated from ApoA or ApoE. The thus produced ApoA or ApoE can be used for the manufacture of a medicament in the treatment of atherosclerosis and cardiovascular diseases, sepsis or peripheral atherosclerosis as well as in a method for treatment of atherosclerosis and cardiovascular diseases, sepsis or peripheral atherosclerosis when administered in a therapeutically effective amount.

    摘要翻译： 本发明涉及一种用于纯化载脂蛋白A（ApoA）或载脂蛋白E（ApoE）的组合物，所述组合物包含第一和第二聚合物材料，其中第一和第二聚合物材料在初级水溶液中是不混溶的， 并且其中所述第二聚合物材料是两亲性和水溶性的。 本发明还涉及通过首先混合ApoA或ApoE，所述组合物含有第一和第二聚合物材料和水，来纯化ApoA或ApoE或其变体或混合物的方法。 将得到的初级水溶液保持足够长的时间基本上分离所形成的相，并除去含有第二聚合物材料的相和ApoA或ApoE的主要部分。 随后，将第二聚合物材料与ApoA或ApoE分离。 由此产生的ApoA或ApoE可用于制备治疗动脉粥样硬化和心血管疾病，败血症或外周动脉粥样硬化的药物，以及用于治疗动脉粥样硬化和心血管疾病，败血症或外周动脉粥样硬化的方法 治疗有效量。

    Recovery of plasmids in an aqueous two-phase system
    7.
    发明申请
    Recovery of plasmids in an aqueous two-phase system 失效

    公开(公告)号：US20060166349A1

    公开(公告)日：2006-07-27

    申请号：US10526089

    申请日：2003-06-17

    申请人： Cecilia Kepka ,  Jenny Rhodin ,  Folke Tjerneld

    发明人： Cecilia Kepka ,  Jenny Rhodin ,  Folke Tjerneld

    IPC分类号： C12N15/63

    CPC分类号： C12N15/1006

    摘要： The present invention is a method for the purification of plasmid DNA comprising to provide a composition comprising a first polymer having inverse solubility characteristics and a second polymer immiscible in the first polymer; contacting said solution with an aqueous solution comprising plasmid DNA; providing phase separation and isolating the aqueous phase; and increasing the temperature of the isolated phase to a temperature above the cloud point of the first polymer and below the temperature where plasmid DNA is degraded and subsequently isolating the aqueous phase so formed. The invention also encompasses a kit for purification of plasmid DNA as described above.

    Separation method utilizing liquid-liquid partition
    8.
    发明授权
    Separation method utilizing liquid-liquid partition 失效
    利用液 - 液分隔的分离方法

    公开(公告)号：US06454950B1

    公开(公告)日：2002-09-24

    申请号：US09869657

    申请日：2001-10-03

    申请人： Folke Tjerneld ,  Josefine Persson ,  Hans Olof Johansson

    发明人： Folke Tjerneld ,  Josefine Persson ,  Hans Olof Johansson

    IPC分类号： B01D1104

    CPC分类号： C07K1/20

    摘要： A method for the separation of a predetermined compound comprising i) partitioning the compound in a two-phase (system A) in which at least one of the phases is rich (phase 1) and the other is poor (phase 2) in a thermoseparating polymer (I), ii) collecting one phase (phase 1 or phase 2) containing said compound, and iii if desired, further working up said compound from the phase collected in step ii, characterized a) in that polymer (I) is a micell-forming thermoseparating polymer, and b) in that phase 2 possibly contains at least one agent that is cloud point-decreasing for thermoseparating polymer (I), such as a polymer (II) that is incompatible with polymer (I) or a salt.

    摘要翻译： 一种用于分离预定化合物的方法，包括：i）在热分离中将化合物在两相（体系A）中分配，其中至少一个相富含（相1）而另一个较差（相2） 聚合物（I），ii）收集含有所述化合物的一相（相1或相2），如果需要，iii，从步骤ii中收集的相进一步处理所述化合物，其特征在于：a）聚合物（I）为 胶束形成热分离聚合物，和b）在该阶段2中可能含有至少一种对于热分离聚合物（I）为浊点降低的试剂，例如与聚合物（I）不相容的聚合物（II）或盐 。

    Polymer conjugates of polyethylene glycols or oxides with polyethyleneimine or polypropylenimine for extracting carboxylic acids from solutions
    9.
    发明授权
    Polymer conjugates of polyethylene glycols or oxides with polyethyleneimine or polypropylenimine for extracting carboxylic acids from solutions 失效
    聚乙二醇或氧化物与聚乙烯亚胺或聚丙烯亚胺的聚合物共轭物用于从溶液中提取羧酸

    公开(公告)号：US6130075A

    公开(公告)日：2000-10-10

    申请号：US296255

    申请日：1999-04-22

    申请人： Jordi Planas ,  Folke Tjerneld ,  Barbel Hahn-Hagerdal

    发明人： Jordi Planas ,  Folke Tjerneld ,  Barbel Hahn-Hagerdal

    IPC分类号： C07C51/48 ,  C12P7/00 ,  C12P7/40 ,  C12P7/48 ,  C12P7/56

    CPC分类号： C07C51/48

    摘要： The invention provides novel polymer conjugates that are especially useful in the separation of carboxylic acids from complex fermentation compositions. The polymer conjugates comprise polyethylene glycols or oxides and polyethyleneimine or polypropyleneimine in various ratios. The novel polymer conjugates permit enhanced extraction of carboxylic acids from fermentation systems not only through their use in partitioning the carboxylic acids from various polymeric components of the fermentation system, but also through their use in adjusting the pH of the system for optimal performance and separation.

    摘要翻译： 本发明提供了新颖的聚合物共轭物，其特别可用于从复合发酵组合物中分离羧酸。 聚合物共轭物包括各种比例的聚乙二醇或氧化物和聚乙烯亚胺或聚丙烯亚胺。 新型聚合物共轭物允许从发酵系统中增强羧酸的提取，不仅通过其用于从发酵系统的各种聚合物组分分配羧酸，而且还通过其用于调节系统的pH以获得最佳性能和分离。

    Recovery of plasmids in an aqueous two-phase system
    10.
    发明授权
    Recovery of plasmids in an aqueous two-phase system 失效
    在两相水相体系中回收质粒

    公开(公告)号：US07553658B2

    公开(公告)日：2009-06-30

    申请号：US10526089

    申请日：2003-06-17

    申请人： Cecilia Jansson Kepka ,  Jenny Rhodin Edsō ,  Folke Tjerneld

    发明人： Cecilia Jansson Kepka ,  Jenny Rhodin Edsō ,  Folke Tjerneld

    IPC分类号： C12N1/08 ,  C12N15/64 ,  C12N15/10

    CPC分类号： C12N15/1006

    摘要： The present invention is a method for the purification of plasmid DNA comprising to provide a composition comprising a first polymer having inverse solubility characteristics and a second polymer immiscible in the first polymer; contacting said solution with an aqueous solution comprising plasmid DNA; providing phase separation and isolating the aqueous phase; and increasing the temperature of the isolated phase to a temperature above the cloud point of the first polymer and below the temperature where plasmid DNA is degraded and subsequently isolating the aqueous phase so formed. The invention also encompasses a kit for purification of plasmid DNA as described above.

    摘要翻译： 本发明是用于纯化质粒DNA的方法，其包括提供包含具有相反溶解度特征的第一聚合物和在第一聚合物中不混溶的第二聚合物的组合物; 使所述溶液与包含质粒DNA的水溶液接触; 提供相分离和分离水相; 并将分离相的温度升高到高于第一聚合物的浊点的温度，并且低于质粒DNA降解的温度，随后分离如此形成的水相。 本发明还包括如上所述的用于纯化质粒DNA的试剂盒。