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    Chimeric glycoproteins and pseudotyped lentiviral vectors
    4.
    发明授权
    Chimeric glycoproteins and pseudotyped lentiviral vectors 有权
    嵌合糖蛋白和假型慢病毒载体

    公开(公告)号：US08338168B2

    公开(公告)日：2012-12-25

    申请号：US10512474

    申请日：2003-04-25

    申请人： Didier Trono ,  Francois-Loic Cosset ,  Virginie Sandrin ,  Bertrand Boson ,  Didier Negre ,  Patrick Salmon

    发明人： Didier Trono ,  Francois-Loic Cosset ,  Virginie Sandrin ,  Bertrand Boson ,  Didier Negre ,  Patrick Salmon

    IPC分类号： C12N15/00 ,  C12N15/64

    CPC分类号： C12N15/86 ,  A61K48/00 ,  C07K14/005 ,  C07K2319/00 ,  C07K2319/03 ,  C12N7/045 ,  C12N2510/02 ,  C12N2740/13022 ,  C12N2740/13043 ,  C12N2740/15043 ,  C12N2740/15045 ,  C12N2810/60 ,  C12N2830/50

    摘要： The present invention provides improved chimeric glycoproteins (GPs) and improved lentiviral vectors pseudotyped with those glycoproteins. Also provided are methods and compositions for making such glycoproteins and vectors, and improved methods of in vitro and in vivo transduction of cells with such vectors. Improved chimeric GPs encode the extracellular and transmembrane domains of GALV or RD114 GPs fused to the cytoplasmic tail of MLV-A GP. Vectors pseudotyped with these GAL V/TR and RD 114/TR GP chimeras have significantly higher titers than vectors coated with the parental GPs. Additionally, RD114/TR-pseudotyped vectors are efficiently concentrated and are resistant to inactivation induced by the complement of both human and macaque sera. RD114 GP-pseudotyped lentiviral vectors have particular utility for in vivo gene transfer applications.

    摘要翻译： 本发明提供改进的嵌合糖蛋白（GP）和用这些糖蛋白假型分类的改良的慢病毒载体。 还提供了用于制备这种糖蛋白和载体的方法和组合物，以及用这些载体体外和体内转导细胞的改进方法。 改进的嵌合GP编码与MLV-A GP的细胞质尾融合的GALV或RD114GP的细胞外和跨膜结构域。 与这些GAL V / TR和RD 114 / TR GP嵌合体假型分离的载体具有比用亲本GP包被的载体更高的滴度。 此外，RD114 / TR-假型载体有效浓缩，并且对由人和猕猴血清的补体诱导的失活具有抗性。 RD114 GP-假型慢病毒载体对于体内基因转移应用具有特殊的用途。

    Chimeric glycoproteins and pseudotyped lentiviral vectors
    9.
    发明申请
    Chimeric glycoproteins and pseudotyped lentiviral vectors 有权
    嵌合糖蛋白和假型慢病毒载体

    公开(公告)号：US20060257366A1

    公开(公告)日：2006-11-16

    申请号：US10512474

    申请日：2003-04-25

    申请人： Didier Trono ,  Francois-Loic Cosset ,  Virginie Sandrin ,  Didier Negre ,  Patrick Salmon

    发明人： Didier Trono ,  Francois-Loic Cosset ,  Virginie Sandrin ,  Didier Negre ,  Patrick Salmon

    IPC分类号： C12N5/08 ,  A61K48/00 ,  C12N15/86

    CPC分类号： C12N15/86 ,  A61K48/00 ,  C07K14/005 ,  C07K2319/00 ,  C07K2319/03 ,  C12N7/045 ,  C12N2510/02 ,  C12N2740/13022 ,  C12N2740/13043 ,  C12N2740/15043 ,  C12N2740/15045 ,  C12N2810/60 ,  C12N2830/50

    摘要： The present invention provides improved chimeric glycoproteins (GPs) and improved lentiviral vectors pseudotyped with those glycoproteins. Also provided are methods and compositions for making such glycoproteins and vectors, and improved methods of in vitro and in vivo transduction of cells with such vectors. Improved chimeric GPs encode the extracellular and transmembrane domains of GALV or RD114 GPs fused to the cytoplasmic tail of MLV-A GP. Vectors pseudotyped with these GAL V/TR and RD 114/TR GP chimeras have significantly higher titers than vectors coated with the parental GPs. Additionally, RD114/TR-pseudotyped vectors are efficiently concentrated and are resistant to inactivation induced by the complement of both human and macaque sera. RD114 GP-pseudotyped lentiviral vectors have particular utility for in vivo gene transfer applications.

    摘要翻译： 本发明提供改进的嵌合糖蛋白（GP）和用这些糖蛋白假型分类的改良的慢病毒载体。 还提供了用于制备这种糖蛋白和载体的方法和组合物，以及用这些载体体外和体内转导细胞的改进方法。 改进的嵌合GP编码与MLV-A GP的细胞质尾融合的GALV或RD114GP的细胞外和跨膜结构域。 与这些GAL V / TR和RD 114 / TR GP嵌合体假型分离的载体具有比用亲本GP包被的载体更高的滴度。 此外，RD114 / TR-假型载体有效浓缩，并且对由人和猕猴血清的补体诱导的失活具有抗性。 RD114 GP-假型慢病毒载体对于体内基因转移应用具有特殊的用途。