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18 条记录 (耗时 0.019 秒)

    Restriction enzyme mediated adapter
    1.
    发明授权
    Restriction enzyme mediated adapter 有权
    限制酶介导的适配器

    公开(公告)号：US06258539B1

    公开(公告)日：2001-07-10

    申请号：US09303774

    申请日：1999-04-30

    申请人： Michael W. Hunkapiller ,  John H. Richards

    发明人： Michael W. Hunkapiller ,  John H. Richards

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6837 ,  C12Q1/6809 ,  C12Q1/6874 ,  C12Q2565/501 ,  C12Q2525/191 ,  C12Q2521/313 ,  C12Q2525/179 ,  C12Q2525/161

    摘要： The present invention relate to methods and compositions for simultaneously analyzing multiple different polynucleotides of a polynucleotide composition comprising multiple diverse polynucleotide sequences. The subject methods and compositions may also be applied to analyze or identify single polynucleotides; however, the subject methods and compositions are particularly useful for analyzing large diverse populations of polynucleotides, e.g., cDNA libraries. Most embodiments of the invention involve hybridizing terminus probes (of known base sequence) to adapter-modified restriction fragment generated from polynucleotide for analysis, and subsequently joining the terminus probes and internal fragment probes to each other. The terminus probe hybridizes to bases of restriction endonuclease recognition site present at the terminus of a restriction fragment generated from the polynucleotide for analysis. The terminus probes and internal fragment probes may be marked so as to facilitate the simultaneous testing of multiple polynucleotides for the presence of many possible nucleotide base sequences. The identity or expression of a particular polynucleotide of interest may be ascertained (or at least partially determined) by producing a short identifier sequence derived from the nucleotide base sequence information obtained from (1) the hybridization of a terminus probe, and (2) the recognition site of a restriction endonuclease used to generate the polynucleotide molecule of interest. Multiple identification sequences may be obtained in parallel, thereby permitting the rapid characterization of a large number of diverse polynucleotides. Parallel processing may be achieved by differentially marking terminus probes or internal fragment probes. Parallel processing may be achieved by using ordered arrays of oligonucleotides that are terminus probes.

    摘要翻译： 本发明涉及用于同时分析包含多种不同多核苷酸序列的多核苷酸组合物的多种不同多核苷酸的方法和组合物。 主题方法和组合物也可以用于分析或鉴定单个多核苷酸; 然而，本发明的方法和组合物特别可用于分析大量不同种群的多核苷酸，例如cDNA文库。 本发明的大多数实施方案涉及将已知碱基序列的末端探针与由多核苷酸产生的衔接子修饰的限制性片段杂交用于分析，然后将末端探针和内部片段探针彼此连接。 末端探针与存在于由多核苷酸产生的限制性片段的末端存在的限制性内切核酸酶识别位点的碱基杂交，用于分析。 可以标记末端探针和内部片段探针，以促进多个多核苷酸的同时测试以存在许多可能的核苷酸碱基序列。 通过产生源自从（1）末端探针的杂交获得的核苷酸碱基序列信息的短标识符序列，可以确定（或至少部分地确定）特定感兴趣的多核苷酸的身份或表达，和（2） 用于产生感兴趣的多核苷酸分子的限制性内切核酸酶的识别位点。 可以并行获得多个鉴定序列，从而允许快速表征大量不同多核苷酸。 通过差异标记末端探针或内部片段探针可以实现并行处理。 可以通过使用作为末端探针的寡核苷酸的有序阵列来实现并行处理。

    Adapter directed expression analysis
    2.
    发明授权
    Adapter directed expression analysis 有权

    公开(公告)号：US06232067B1

    公开(公告)日：2001-05-15

    申请号：US09135381

    申请日：1998-08-17

    申请人： Michael W. Hunkapiller ,  John H. Richards

    发明人： Michael W. Hunkapiller ,  John H. Richards

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6837 ,  C12Q1/6809 ,  C12Q1/6874 ,  C12Q2565/501 ,  C12Q2525/191 ,  C12Q2521/313 ,  C12Q2525/179 ,  C12Q2525/161

    摘要： The present invention relate to methods and compositions for simultaneously analyzing multiple different polynucleotides of a polynucleotide composition comprising multiple diverse polynucleotide sequences. The subject methods and compositions may also be applied to analyze or identify single polynucleotides; however, the subject methods and compositions are particularly useful for analyzing large diverse populations of polynucleotides, e.g., cDNA libraries. Most embodiments of the invention involve hybridizing terminus probes (of known base sequence) and internal fragment probes (of known base sequence) at adjacent positions on an adapter-modified restriction fragment generated from polynucleotide for analysis, and subsequently joining the terminus probes and internal fragment probes to each other. The terminus probe hybridizes to bases of restriction endonuclease recognition site present at the terminus of a restriction fragment generated from the polynucleotide for analysis. Internal fragment probes hybridizes to the same strand of the restriction fragment that the terminus probe hybridizes to and hybridizes to the restriction fragment portion of adapter-modified representative restriction fragments. The terminus probes and internal fragment probes may be marked so as to facilitate the simultaneous testing of multiple polynucleotides for the presence of many possible nucleotide base sequences. The identity or expression of a particular polynucleotide of interest may be ascertained (or at least partially determined) by producing a short identifier sequence derived from the nucleotide base sequence information obtained from (1) the hybridization of a terminus probe and an internal fragment probe, each of known base sequence, at adjacent positions on a polynucleotide of interest, and (2) the recognition site of a restriction endonuclease used to generate the polynucleotide molecule of interest. Multiple identification sequences may be obtained in parallel, thereby permitting the rapid characterization of a large number of diverse polynucleotides. Parallel processing may be achieved by differentially marking terminus probes or internal fragment probes. Parallel processing may be achieved by using ordered arrays of oligonucleotides that are terminus probes.

    System and method for detecting a head positioning error within a computer memory device
    4.
    发明授权
    System and method for detecting a head positioning error within a computer memory device 失效
    用于检测计算机存储装置内的头部定位误差的系统和方法

    公开(公告)号：US06349079B1

    公开(公告)日：2002-02-19

    申请号：US09414745

    申请日：1999-10-08

    申请人： Karl A. Belser ,  Lawrence M. Bryant ,  John H. Richards

    发明人： Karl A. Belser ,  Lawrence M. Bryant ,  John H. Richards

    IPC分类号： G11B700

    CPC分类号： G11B11/10576 ,  G11B7/0901 ,  G11B7/0938 ,  G11B11/10578

    摘要： A system and method are provided for processing signals in a magneto-optical computer memory device to detect mispositioning of a head with respect to a track centerline. A light beam is scanned over a first and a second set of radially offset optically-detectable position marks formed in the rotating medium surface. A detector receives the reflected light beam and responsively generates a position signal having a plurality of pulses corresponding to the position marks. The position signal is then passed through a differentiator circuit. The differentiated position signal may then be applied to low pass filter and resonator circuits, and is subsequently conveyed to a finite time integrator for rectification and detection of the areas of the pulses associated with the first and second set of position marks. Mispositioning of the head is detected by comparing the pulse areas of the first and second set of position marks.

    摘要翻译： 提供了一种用于处理磁光计算机存储装置中的信号的系统和方法，以检测头相对于轨道中心线的误位。 在形成在旋转介质表面中的第一组和第二组径向偏移的光学可检测位置标记上扫描光束。 检测器接收反射光束并且响应地产生具有对应于位置标记的多个脉冲的位置信号。 然后，位置信号通过微分电路。 差分位置信号然后可以被施加到低通滤波器和谐振器电路，并且随后被传送到有限时间积分器，以对与第一和第二组位置标记相关联的脉冲的区域进行整流和检测。 通过比较第一和第二组位置标记的脉冲区域来检测头部的位置。

    Fail safe device for incubator air warmer
    7.
    发明授权
    Fail safe device for incubator air warmer 有权
    孵化器空气保温器故障安全装置

    公开(公告)号：US06483080B2

    公开(公告)日：2002-11-19

    申请号：US09838789

    申请日：2001-04-20

    申请人： John H. Richards ,  Robert M. McDonough

    发明人： John H. Richards ,  Robert M. McDonough

    IPC分类号： A61F700

    CPC分类号： H05B1/025 ,  A61G11/00 ,  H05B3/00 ,  H05B3/50

    摘要： An infant-support heater assembly having a heater element a radiator associated with the heater element and a sensing element in contact with at least a portion of the radiator. The sensing element is separate from the heater element.

    摘要翻译： 一种婴儿支撑加热器组件，其具有加热器元件，与加热器元件相关联的散热器和与散热器的至少一部分接触的感测元件。 传感元件与加热元件分开。

    DNA Polymerases Having Improved Labeled Nucleotide Incorporation Properties
    8.
    发明申请
    DNA Polymerases Having Improved Labeled Nucleotide Incorporation Properties 审中-公开
    具有改进的标记核苷酸掺入特性的DNA聚合酶

    公开(公告)号：US20110244548A1

    公开(公告)日：2011-10-06

    申请号：US13007570

    申请日：2011-01-14

    申请人： John BRANDIS ,  Curtis Bloom ,  John H. Richards

    发明人： John BRANDIS ,  Curtis Bloom ,  John H. Richards

    IPC分类号： C12N9/12

    CPC分类号： C12N9/1252 ,  C12P19/34 ,  C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6869 ,  C12Q2521/101 ,  C12Q2563/107

    摘要： The present invention relates to mutant DNA polymerases that exhibit reduced discrimination against labeled nucleotides into polynucleotides. The DNA polymerases of the invention have at least one mutation in the nucleotide label interaction region of the enzyme such the mutation results in reduced discrimination against labeled nucleotides. The nucleotide label interaction regions is located at portions of the O-helix, (ii) the K helix, and (iii) the inter O-P helical loop of Taq DNA polymerase or analogous positions in other DNA polymerases. In addition to providing novel mutant DNA polymerases, the invention also provides polynucleotides encoding the subject mutant DNA polymerases. The polynucleotides provided may comprise expression vectors for the recombinant production of the mutant polymerases. The invention also provides host cells containing the subject polynucleotides. The invention also includes numerous methods of using the subject DNA polymerases, including uses for chain termination sequencing and PCR. Another aspect of the invention is to provide kits for synthesizing fluorescently labeled polynucleotides in accordance with the methods of the invention. Kits of the invention comprise a mutant DNA polymerase of the invention and a fluorescently labeled nucleotide that exhibits reduced discrimination with respect to the mutant DNA polymerase in the kit.

    摘要翻译： 本发明涉及对标记的核苷酸表现出降低的对多核苷酸的歧视的突变型DNA聚合酶。 本发明的DNA聚合酶在酶的核苷酸标签相互作用区域中具有至少一个突变，因此突变导致对标记核苷酸的歧视减少。 核苷酸标签相互作用区位于O-螺旋的部分，（ii）K螺旋，和（iii）Taq DNA聚合酶的O-P螺旋环或其它DNA聚合酶中的类似位置。 除了提供新的突变型DNA聚合酶之外，本发明还提供了编码本发明突变体DNA聚合酶的多核苷酸。 提供的多核苷酸可以包含用于重组产生突变型聚合酶的表达载体。 本发明还提供含有受试多核苷酸的宿主细胞。 本发明还包括使用主题DNA聚合酶的许多方法，包括用于链终止测序和PCR的用途。 本发明的另一方面是提供根据本发明的方法合成荧光标记的多核苷酸的试剂盒。 本发明的试剂盒包含本发明的突变型DNA聚合酶和荧光标记的核苷酸，其在试剂盒中显示相对于突变型DNA聚合酶的降低的鉴别。