公开(公告)号：US20110158954A1

公开(公告)日：2011-06-30

申请号：US12450017

申请日：2008-03-04

申请人： Mitsuko Ideno ,  Fumiyo Sakai ,  Tatsuji Enoki ,  Ikunoshin Kato

发明人： Mitsuko Ideno ,  Fumiyo Sakai ,  Tatsuji Enoki ,  Ikunoshin Kato

IPC分类号： A61K35/12 ,  A61P31/04 ,  A61P31/12 ,  C12N5/0783 ,  C12N15/86 ,  C12N15/867 ,  C12N15/861

CPC分类号： C12N5/0636 ,  A61K2035/122 ,  C12N2501/58 ,  C12N2501/999 ,  C12N2533/52

摘要： A method for preparing a γδ T cell population, characterized in that the method includes the step of culturing a cell population containing γδ T cells, in the presence of (a) fibronectin, a fibronectin fragment or a mixture thereof and (b) an activating factor of γδ T cells. According to the present invention, a method for preparing a γδ T cell population is provided. The γδ T cell population obtained by the method is suitably used in, for example, immunotherapy. Therefore, the method of the present invention is expected to greatly contribute to a medical field.

摘要翻译： 一种制备γδT细胞群体的方法，其特征在于该方法包括在（a）纤连蛋白，纤连蛋白片段或其混合物存在下培养含有γδT细胞的细胞群的步骤，和（b）活化 γδT细胞因子。 根据本发明，提供了制备γδT细胞群体的方法。 通过该方法获得的γδT细胞群适用于例如免疫治疗。 因此，预期本发明的方法对医疗领域有很大贡献。

公开(公告)号：US08216837B2

公开(公告)日：2012-07-10

申请号：US12303911

申请日：2007-06-07

申请人： Nobuko Muraki ,  Mitsuko Ideno ,  Kinuko Nagamine ,  Fuyuko Takashima ,  Eiji Kobayashi ,  Akiko Kato ,  Takahiro Marui ,  Hiroaki Sagawa ,  Tatsuji Enoki ,  Ikunoshin Kato

发明人： Nobuko Muraki ,  Mitsuko Ideno ,  Kinuko Nagamine ,  Fuyuko Takashima ,  Eiji Kobayashi ,  Akiko Kato ,  Takahiro Marui ,  Hiroaki Sagawa ,  Tatsuji Enoki ,  Ikunoshin Kato

IPC分类号： C12N5/071

CPC分类号： C07K14/78 ,  A61K35/17 ,  A61K2039/5158 ,  C12N5/0636 ,  C12N2501/58 ,  C12N2533/52

摘要： A method of producing lymphocytes characterized by comprising the step of culturing lymphocytes in the presence of a modified recombinant fibronectin fragment which has overlapping parts of the heparin-binding domain of fibronectin. This method makes it possible to achieve a high cell proliferation rate. The lymphocytes obtained thereby are appropriately usable in, for example, adoptive immunotherapy and, therefore, expected as highly useful in the clinical field. Moreover, a novel modified recombinant fibronectin fragment is provided.

摘要翻译： 一种产生淋巴细胞的方法，其特征在于包括在纤连蛋白的肝素结合域的重叠部分的修饰重组纤连蛋白片段存在下培养淋巴细胞的步骤。 该方法可以实现高细胞增殖率。 由此获得的淋巴细胞可适用于例如过继免疫治疗，因此预期在临床领域中非常有用。 此外，提供了新的修饰的重组纤连蛋白片段。

公开(公告)号：US20090221077A1

公开(公告)日：2009-09-03

申请号：US10568745

申请日：2004-08-19

申请人： Mitsuko Ideno ,  Nobuko Muraki ,  Kinuko Ogawa ,  Masayuki Kishimoto ,  Tatsuji Enoki ,  Hiroaki Sagawa ,  Ikunoshin Kato

发明人： Mitsuko Ideno ,  Nobuko Muraki ,  Kinuko Ogawa ,  Masayuki Kishimoto ,  Tatsuji Enoki ,  Hiroaki Sagawa ,  Ikunoshin Kato

IPC分类号： C12N15/86 ,  C12N5/00 ,  C12N5/02 ,  C12N15/00 ,  C07K14/00 ,  C07H21/00

CPC分类号： C12N5/0636 ,  C12N2501/58

摘要： The present invention provides a method for preparing a cytotoxic lymphocyte characterized in that the method comprises the step of carrying out at least one step selected from induction, maintenance and expansion of a cytotoxic lymphocyte using a medium containing serum and plasma at a total concentration of 0% by volume or more and less than 5% by volume, in the presence of fibronectin, a fragment thereof or a mixture thereof.

摘要翻译： 本发明提供了一种制备细胞毒性淋巴细胞的方法，其特征在于该方法包括使用含有总浓度为0的血清和血浆的培养基进行选自诱导，维持和扩增细胞毒性淋巴细胞的至少一个步骤的步骤 在纤维连接蛋白，其片段或其混合物的存在下，以体积计或更多且小于5体积％。

公开(公告)号：US08927273B2

公开(公告)日：2015-01-06

申请号：US10568745

申请日：2004-08-19

申请人： Mitsuko Ideno ,  Nobuko Muraki ,  Kinuko Ogawa ,  Masayuki Kishimoto ,  Tatsuji Enoki ,  Hiroaki Sagawa ,  Ikunoshin Kato

发明人： Mitsuko Ideno ,  Nobuko Muraki ,  Kinuko Ogawa ,  Masayuki Kishimoto ,  Tatsuji Enoki ,  Hiroaki Sagawa ,  Ikunoshin Kato

IPC分类号： C12N5/07 ,  C12N5/0783

CPC分类号： C12N5/0636 ,  C12N2501/58

摘要： The present invention provides a method for preparing a cytotoxic lymphocyte characterized in that the method comprises the step of carrying out at least one step selected from induction, maintenance and expansion of a cytotoxic lymphocyte using a medium containing serum and plasma at a total concentration of 0% by volume or more and less than 5% by volume, in the presence of fibronectin, a fragment thereof or a mixture thereof.

公开(公告)号：US20100255578A1

公开(公告)日：2010-10-07

申请号：US12303911

申请日：2007-06-07

申请人： Nobuko Muraki ,  Mitsuko Ideno ,  Kinuko Nagamine ,  Fuyuko Takashima ,  Eiji Kobayashi ,  Akiko Kato ,  Takahiro Marui ,  Hiroaki Sagawa ,  Tatsuji Enoki ,  Ikunoshin Kato

发明人： Nobuko Muraki ,  Mitsuko Ideno ,  Kinuko Nagamine ,  Fuyuko Takashima ,  Eiji Kobayashi ,  Akiko Kato ,  Takahiro Marui ,  Hiroaki Sagawa ,  Tatsuji Enoki ,  Ikunoshin Kato

IPC分类号： C12N5/078 ,  C07K14/78 ,  C07H21/00

CPC分类号： C07K14/78 ,  A61K35/17 ,  A61K2039/5158 ,  C12N5/0636 ,  C12N2501/58 ,  C12N2533/52

摘要： A method of producing lymphocytes characterized by comprising the step of culturing lymphocytes in the presence of a modified recombinant fibronectin fragment which has overlapping parts of the heparin-binding domain of fibronectin. This method makes it possible to achieve a high cell proliferation rate. The lymphocytes obtained thereby are appropriately usable in, for example, adoptive immunotherapy and, therefore, expected as highly useful in the clinical field. Moreover, a novel modified recombinant fibronectin fragment is provided.

摘要翻译： 一种产生淋巴细胞的方法，其特征在于包括在纤连蛋白的肝素结合域的重叠部分的修饰重组纤连蛋白片段存在下培养淋巴细胞的步骤。 该方法可以实现高细胞增殖率。 由此获得的淋巴细胞可适用于例如过继免疫治疗，因此预期在临床领域中非常有用。 此外，提供了新的修饰的重组纤连蛋白片段。

公开(公告)号：US20100247579A1

公开(公告)日：2010-09-30

申请号：US12599541

申请日：2008-05-08

申请人： Hiroshi Shiku ,  Shinichi Kageyama ,  Shigehisa Kitano ,  Mitsuko Ideno ,  Keisuke Tomita ,  Tatsuji Enoki ,  Kazutoh Takesako ,  Ikunoshin Kato

发明人： Hiroshi Shiku ,  Shinichi Kageyama ,  Shigehisa Kitano ,  Mitsuko Ideno ,  Keisuke Tomita ,  Tatsuji Enoki ,  Kazutoh Takesako ,  Ikunoshin Kato

IPC分类号： A61K39/00 ,  A61K35/12 ,  C12N5/00 ,  A61P35/04

CPC分类号： A61K35/14 ,  A61K39/0011 ,  A61K45/06 ,  C12N5/0636 ,  C12N2501/2302 ,  A61K2300/00

摘要： The present invention relates to a method for treatment of cancer, which comprises the following steps (A) and (B): (A) applying a treatment which induces the reduction in lymphocytes to a patient; and (B), subsequent to step (A), administering lymphocytes to the patient promptly. The present invention also relates to a cancer therapeutic agent and a cancer treatment kit for use in the method. When the method is used as an immunoreconstructive therapy, the decrease in immunologic competence which may be caused by the reduction in lymphocytes can be avoided and, therefore, the risk of the occurrence of an infectious disease can be decreased.

摘要翻译： 本发明涉及一种治疗癌症的方法，其包括以下步骤（A）和（B）：（A）应用诱导淋巴细胞减少至患者的治疗方法; 和（B），在步骤（A）之后，迅速给予患者淋巴细胞。 本发明还涉及用于该方法的癌症治疗剂和癌症治疗试剂盒。 当该方法用作免疫构建性治疗时，可以避免可能由淋巴细胞减少引起的免疫能力的降低，因此可以降低感染性疾病发生的风险。

公开(公告)号：US20100068192A1

公开(公告)日：2010-03-18

申请号：US11992661

申请日：2006-09-27

申请人： Tatsuji Enoki ,  Akiko Kato ,  Mitsuko Ideno ,  Hiroaki Sagawa ,  Ikunoshin Kato

发明人： Tatsuji Enoki ,  Akiko Kato ,  Mitsuko Ideno ,  Hiroaki Sagawa ,  Ikunoshin Kato

IPC分类号： A61K35/12 ,  C12N5/00 ,  C12N5/02

CPC分类号： C12N5/0636 ,  A61K35/17 ,  A61K38/19 ,  A61K39/00 ,  A61K39/0011 ,  A61K2039/5158 ,  C12N15/86 ,  C12N2501/515 ,  C12N2501/58 ,  C12N2740/10043 ,  A61K2300/00

摘要： A method for preparing a T cell population, wherein the T cell population expresses CD45RA and expresses at least one selected from the group consisting of CD62L, CCR7, CD27, and CD28, characterized in that the method comprises the step of culturing a cell population comprising a T cell, in the presence of fibronectin, a fragment thereof or a mixture thereof.

摘要翻译： 一种制备T细胞群体的方法，其中所述T细胞群表达CD45RA并表达选自CD62L，CCR7，CD27和CD28的至少一种，其特征在于所述方法包括培养细胞群的步骤，所述细胞群包含 在纤连蛋白存在下，其T细胞，其片段或其混合物。

公开(公告)号：US09062287B2

公开(公告)日：2015-06-23

申请号：US13394398

申请日：2010-09-10

申请人： Mitsuko Ideno ,  Mie Yabuuchi ,  Meiko Jin ,  Masae Sato ,  Naoko Ashida ,  Tatsuji Enoki

发明人： Mitsuko Ideno ,  Mie Yabuuchi ,  Meiko Jin ,  Masae Sato ,  Naoko Ashida ,  Tatsuji Enoki

IPC分类号： C12N5/0783 ,  A61K35/12

CPC分类号： C12N5/0646 ,  A61K2035/124 ,  C12N2500/72 ,  C12N2501/2302 ,  C12N2501/24 ,  C12N2501/515 ,  C12N2502/1114

摘要： A process for producing a cell mass containing natural killer cells, characterized by involving a step of carrying out the expansion culture of a cell mass containing natural killer cells and/or cells capable of being differentiated into natural killer cells in the presence of a biological response modifier and cells that has been so treated as to lose a proliferation capability, and others.

摘要翻译： 一种生产含有天然杀伤细胞的细胞块的方法，其特征在于包括在存在生物反应的情况下进行包含天然杀伤细胞和/或能够分化为天然杀伤细胞的细胞的细胞团的扩增培养的步骤 修饰剂和已被如此处理以丧失增殖能力的细胞等。

公开(公告)号：US20120171173A1

公开(公告)日：2012-07-05

申请号：US13394398

申请日：2010-09-10

申请人： Mitsuko Ideno ,  Mie Yabuuchi ,  Meiko Jin ,  Masae Sato ,  Naoko Ashida ,  Tatsuji Enoki

发明人： Mitsuko Ideno ,  Mie Yabuuchi ,  Meiko Jin ,  Masae Sato ,  Naoko Ashida ,  Tatsuji Enoki

IPC分类号： A61K35/14 ,  C12N5/0783

CPC分类号： C12N5/0646 ,  A61K2035/124 ,  C12N2500/72 ,  C12N2501/2302 ,  C12N2501/24 ,  C12N2501/515 ,  C12N2502/1114

摘要： A process for producing a cell mass containing natural killer cells, characterized by involving a step of carrying out the expansion culture of a cell mass containing natural killer cells and/or cells capable of being differentiated into natural killer cells in the presence of a biological response modifier and cells that has been so treated as to lose a proliferation capability, and others.

摘要翻译： 一种生产含有天然杀伤细胞的细胞块的方法，其特征在于包括在存在生物反应的情况下进行包含天然杀伤细胞和/或能够分化为天然杀伤细胞的细胞的细胞团的扩增培养的步骤 修饰剂和已被如此处理以丧失增殖能力的细胞等。

公开(公告)号：US20050042208A1

公开(公告)日：2005-02-24

申请号：US10486512

申请日：2002-08-15

申请人： Hiroaki Sagawa ,  Mitsuko Ideno ,  Ikunoshin Kato

发明人： Hiroaki Sagawa ,  Mitsuko Ideno ,  Ikunoshin Kato

IPC分类号： A61K39/395 ,  A61P31/12 ,  A61P31/22 ,  A61P35/00 ,  A61P37/04 ,  C07K14/78 ,  C12N5/02 ,  C12N5/07 ,  C12N5/0783 ,  C12N15/09 ,  C12N5/08

CPC分类号： C12N5/0636 ,  A61K2039/515 ,  A61K2039/5158 ,  C12N2501/105 ,  C12N2501/12 ,  C12N2501/23 ,  C12N2501/515 ,  C12N2501/58 ,  C12N2501/599 ,  C12N2501/905

摘要： The present invention is a method for inducing cytotoxic T cell having an antigen-specific cytotoxic activity, a method for maintaining the cell, a method for continuously culturing the cell or a method for expanding the cell, comprising the step of culturing a cytotoxic T cell in the presence of at least one substance selected from the group consisting of (A) a substance having a binding activity to CD44; (B) a substance capable of regulating a signal emitted by binding a CD44 ligand to CD44; (C) a substance capable of inhibiting binding of a growth factor to a growth factor receptor; (D) a substance capable of regulating a signal emitted by binding of a growth factor to a growth factor receptor; and (E) fibronectin, a fragment thereof or a mixture thereof.

摘要翻译： 本发明是诱导具有抗原特异性细胞毒活性的细胞毒性T细胞的方法，用于维持细胞的方法，连续培养细胞的方法或扩增细胞的方法，包括培养细胞毒性T细胞的步骤 在至少一种选自（A）与CD44具有结合活性的物质的物质的存在下， （B）能够调节通过将CD44配体结合CD44而发射的信号的物质; （C）能够抑制生长因子与生长因子受体结合的物质; （D）能够调节由生长因子与生长因子受体结合而发出的信号的物质; 和（E）纤连蛋白，其片段或其混合物。