公开(公告)号：US11647753B2

公开(公告)日：2023-05-16

申请号：US16434854

申请日：2019-06-07

Applicant: Monsanto Technology LLC

Inventor： James A. Baum ,  Allen T. Christian ,  Artem Evdokimov ,  Farhad Moshiri ,  Lisa Marie Weaver ,  Haitao Zhang

IPC: A01N63/60 ,  C12N15/67 ,  A01H3/00 ,  C12N15/72 ,  C12N9/22

CPC classification number: A01N63/60 ,  A01H3/00 ,  C12N9/22 ,  C12N15/67 ,  C12N15/72 ,  A01N63/60 ,  A01N63/14

Abstract: Compositions and methods for efficiently producing and delivering double stranded RNA (dsRNA) are provided. Vector constructs useful for in vitro and in vivo expression of dsRNA are described. Also described are cell expression systems for efficient and cost-effective production of dsRNA in living cells and methods and compositions for providing the expressed dsRNA to target organisms. The described compositions and methods can be used to produce RNA molecules for screening or other uses, and to amplify RNA sequences for analysis.

公开(公告)号：US09814243B2

公开(公告)日：2017-11-14

申请号：US14901003

申请日：2014-03-13

Applicant: Monsanto Technology LLC

Inventor： James A. Baum ,  Allen T. Christian ,  Artem Evdokimov ,  Farhad Moshiri ,  Lisa Marie Weaver ,  Haitao Zhang

IPC: C07H21/04 ,  C12N15/67 ,  A01N63/02 ,  A01H3/00 ,  A01N63/00 ,  C12N15/72 ,  C12N9/22

CPC classification number: A01N63/02 ,  A01H3/00 ,  A01N63/00 ,  C12N9/22 ,  C12N15/67 ,  C12N15/72

Abstract: Compositions and methods for efficiently producing and delivering double stranded RNA (dsRNA) are provided. Vector constructs useful for in vitro and in vivo expression of dsRNA are described. Also described are cell expression systems for efficient and cost-effective production of dsRNA in living cells and methods and compositions for providing the expressed dsRNA to target organisms. The described compositions and methods can be used to produce RNA molecules for screening or other uses, and to amplify RNA sequences for analysis.

公开(公告)号：US09445603B2

公开(公告)日：2016-09-20

申请号：US14208788

申请日：2014-03-13

Applicant: Monsanto Technology LLC

Inventor： James A. Baum ,  Allen T. Christian ,  Artem Evdokimov ,  Farhad Moshiri ,  Lisa Marie Weaver ,  Haitao Zhang

IPC: A01N63/00 ,  A01N65/00 ,  C12N1/20 ,  C12N15/00 ,  C07H21/02 ,  A01N63/02 ,  C12N15/67 ,  A01H3/00

CPC classification number: A01N63/02 ,  A01H3/00 ,  A01N63/00 ,  C12N9/22 ,  C12N15/67 ,  C12N15/72

Abstract: Compositions and methods for efficiently producing and delivering double stranded RNA (dsRNA) are provided. Vector constructs useful for in vitro and in vivo expression of dsRNA are described. Also described are cell expression systems for efficient and cost-effective production of dsRNA in living cells and methods and compositions for providing the expressed dsRNA to target organisms. The described compositions and methods can be used to produce RNA molecules for screening or other uses, and to amplify RNA sequences for analysis.

Abstract translation: 提供了有效生产和递送双链RNA（dsRNA）的组合物和方法。 描述了可用于dsRNA的体外和体内表达的载体构建体。 还描述了用于在活细胞中有效和成本有效地生产dsRNA的细胞表达系统，以及用于将表达的dsRNA提供给靶生物的方法和组合物。 所述的组合物和方法可用于产生用于筛选或其它用途的RNA分子，并扩增用于分析的RNA序列。

公开(公告)号：US10757947B2

公开(公告)日：2020-09-01

申请号：US16103451

申请日：2018-08-14

Applicant: Monsanto Technology LLC

Inventor： James A. Baum ,  Allen T. Christian ,  Artem Evdokimov ,  Farhad Moshiri ,  Lisa Marie Weaver ,  Haitao Zhang

IPC: A01N63/10 ,  C12N9/22 ,  C12N15/72 ,  A01N63/00 ,  A01H3/00 ,  C12N15/67

Abstract: Compositions and methods for efficiently producing and delivering double stranded RNA (dsRNA) are provided. Vector constructs useful for in vitro and in vivo expression of dsRNA are described. Also described are cell expression systems for efficient and cost-effective production of dsRNA in living cells and methods and compositions for providing the expressed dsRNA to target organisms. The described compositions and methods can be used to produce RNA molecules for screening or other uses, and to amplify RNA sequences for analysis.

公开(公告)号：US20160145630A1

公开(公告)日：2016-05-26

申请号：US14901003

申请日：2014-03-13

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： James A. Baum ,  Allen T. Christian ,  Artem Evdokimov ,  Farhad Moshiri ,  Lisa Marie Weaver ,  Haitao Zhang

IPC: C12N15/72 ,  A01N63/00 ,  A01N63/02

CPC classification number: A01N63/02 ,  A01H3/00 ,  A01N63/00 ,  C12N9/22 ,  C12N15/67 ,  C12N15/72

Abstract: Compositions and methods for efficiently producing and delivering double stranded RNA (dsRNA) are provided. Vector constructs useful for in vitro and in vivo expression of dsRNA are described. Also described are cell expression systems for efficient and cost-effective production of dsRNA in living cells and methods and compositions for providing the expressed dsRNA to target organisms. The described compositions and methods can be used to produce RNA molecules for screening or other uses, and to amplify RNA sequences for analysis.

Abstract translation: 提供了有效生产和递送双链RNA（dsRNA）的组合物和方法。 描述了可用于dsRNA的体外和体内表达的载体构建体。 还描述了用于在活细胞中有效和成本有效地生产dsRNA的细胞表达系统，以及用于将表达的dsRNA提供给靶生物的方法和组合物。 所述的组合物和方法可用于产生用于筛选或其它用途的RNA分子，并扩增用于分析的RNA序列。

公开(公告)号：US20140271559A1

公开(公告)日：2014-09-18

申请号：US14208788

申请日：2014-03-13

Applicant: Monsanto Technology LLC

Inventor： James A. Baum ,  Allen T. Christian ,  Artem Evdokimov ,  Farhad Moshiri ,  Lisa Marie Weaver ,  Haitao Zhang

IPC: A01N63/02

CPC classification number: A01N63/02 ,  A01H3/00 ,  A01N63/00 ,  C12N9/22 ,  C12N15/67 ,  C12N15/72

Abstract: Compositions and methods for efficiently producing and delivering double stranded RNA (dsRNA) are provided. Vector constructs useful for in vitro and in vivo expression of dsRNA are described. Also described are cell expression systems for efficient and cost-effective production of dsRNA in living cells and methods and compositions for providing the expressed dsRNA to target organisms. The described compositions and methods can be used to produce RNA molecules for screening or other uses, and to amplify RNA sequences for analysis.

Abstract translation: 提供了有效生产和递送双链RNA（dsRNA）的组合物和方法。 描述了可用于dsRNA的体外和体内表达的载体构建体。 还描述了用于在活细胞中有效和成本有效地生产dsRNA的细胞表达系统，以及用于将表达的dsRNA提供给靶生物的方法和组合物。 所述的组合物和方法可用于产生用于筛选或其它用途的RNA分子，并扩增用于分析的RNA序列。

公开(公告)号：US20190000089A1

公开(公告)日：2019-01-03

申请号：US16103451

申请日：2018-08-14

Applicant: Monsanto Technology LLC

Inventor： James A. Baum ,  Allen T. Christian ,  Artem Evdokimov ,  Farhad Moshiri ,  Lisa Marie Weaver ,  Haitao Zhang

IPC: A01N63/02 ,  C12N9/22 ,  C12N15/72 ,  A01H3/00 ,  C12N15/67 ,  A01N63/00

Abstract: Compositions and methods for efficiently producing and delivering double stranded RNA (dsRNA) are provided. Vector constructs useful for in vitro and in vivo expression of dsRNA are described. Also described are cell expression systems for efficient and cost-effective production of dsRNA in living cells and methods and compositions for providing the expressed dsRNA to target organisms. The described compositions and methods can be used to produce RNA molecules for screening or other uses, and to amplify RNA sequences for analysis.

公开(公告)号：US10334858B2

公开(公告)日：2019-07-02

申请号：US15729114

申请日：2017-10-10

Applicant: Monsanto Technology LLC

Inventor： James A. Baum ,  Allen T. Christian ,  Artem Evdokimov ,  Farhad Moshiri ,  Lisa Marie Weaver ,  Haitao Zhang

IPC: C07H21/04 ,  C12N15/67 ,  A01N63/02 ,  A01H3/00 ,  A01N63/00 ,  C12N15/72 ,  C12N9/22

Abstract: Compositions and methods for efficiently producing and delivering double stranded RNA (dsRNA) are provided. Vector constructs useful for in vitro and in vivo expression of dsRNA are described. Also described are cell expression systems for efficient and cost-effective production of dsRNA in living cells and methods and compositions for providing the expressed dsRNA to target organisms. The described compositions and methods can be used to produce RNA molecules for screening or other uses, and to amplify RNA sequences for analysis.

公开(公告)号：US10070652B2

公开(公告)日：2018-09-11

申请号：US15237214

申请日：2016-08-15

Applicant: Monsanto Technology LLC

Inventor： James A. Baum ,  Allen T. Christian ,  Artem Evdokimov ,  Farhad Moshiri ,  Lisa Marie Weaver ,  Haitao Zhang

IPC: A01N63/00 ,  A01N65/00 ,  C12N1/20 ,  C12N15/00 ,  C07H21/02 ,  A01N63/02 ,  C12N15/67 ,  A01H3/00 ,  C12N15/72 ,  C12N9/22

Abstract: Compositions and methods for efficiently producing and delivering double stranded RNA (dsRNA) are provided. Vector constructs useful for in vitro and in vivo expression of dsRNA are described. Also described are cell expression systems for efficient and cost-effective production of dsRNA in living cells and methods and compositions for providing the expressed dsRNA to target organisms. The described compositions and methods can be used to produce RNA molecules for screening or other uses, and to amplify RNA sequences for analysis.

公开(公告)号：US20170035056A1

公开(公告)日：2017-02-09

申请号：US15237214

申请日：2016-08-15

Applicant: Monsanto Technology LLC

Inventor： James A. Baum ,  Allen T. Christian ,  Artem Evdokimov ,  Farhad Moshiri ,  Lisa Marie Weaver ,  Haitao Zhang

IPC: A01N63/02 ,  C12N15/72 ,  A01N63/00 ,  C12N9/22

CPC classification number: A01N63/02 ,  A01H3/00 ,  A01N63/00 ,  C12N9/22 ,  C12N15/67 ,  C12N15/72

Abstract: Compositions and methods for efficiently producing and delivering double stranded RNA (dsRNA) are provided. Vector constructs useful for in vitro and in vivo expression of dsRNA are described. Also described are cell expression systems for efficient and cost-effective production of dsRNA in living cells and methods and compositions for providing the expressed dsRNA to target organisms. The described compositions and methods can be used to produce RNA molecules for screening or other uses, and to amplify RNA sequences for analysis.

Abstract translation: 提供了有效生产和递送双链RNA（dsRNA）的组合物和方法。 描述了可用于dsRNA的体外和体内表达的载体构建体。 还描述了用于在活细胞中有效和成本有效地生产dsRNA的细胞表达系统，以及用于将表达的dsRNA提供给靶生物的方法和组合物。 所述的组合物和方法可用于产生用于筛选或其它用途的RNA分子，并扩增用于分析的RNA序列。