公开(公告)号：US08080402B2

公开(公告)日：2011-12-20

申请号：US11622158

申请日：2007-01-11

申请人： Moon-Hee Sung ,  Seung-Pyo Hong ,  Jong-Su Lee ,  Chang-Min Jung ,  Kyung-Soo Hahm ,  Dong-Gun Lee ,  Yoon Kyung Park ,  Chul-Joong Kim ,  Ha-Ryoung Poo

发明人： Moon-Hee Sung ,  Seung-Pyo Hong ,  Jong-Su Lee ,  Chang-Min Jung ,  Kyung-Soo Hahm ,  Dong-Gun Lee ,  Yoon Kyung Park ,  Chul-Joong Kim ,  Ha-Ryoung Poo

IPC分类号： C12N15/00 ,  C12N1/21 ,  A61K48/00

CPC分类号： C07K14/32

摘要： The present invention relates to a method for expressing each of peptide antibiotics P5 3 and Anal3 35 having amphiphilicity and showing antibacterial, antifungal and anticancer activities 61, 63, 65, 67, 69, 71, on the microbial surface, using a vector containing outer membrane protein genes (pgsBCA) that are derived from Bacillus sp. strains and involved in the synthesis of poly-gamma-glutamate. Moreover, the present invention relates to lactic acid-forming bacteria having each of the peptide antibiotics P5 15 and Anal3 43 expressed on their surface, and the use thereof. According to the present invention, the peptide antibiotics can be expressed on the surface of various microorganisms transformed with the surface expression vectors. The inventive method for the surface expression of the peptide antibiotics allows the peptide antibiotics to be mass-produced without a purification process. Thus, the inventive method has very high industrial applicability. Further, the present invention can be applied to other peptide antibiotics besides P5 3 and Anal 3 35.

摘要翻译： 本发明涉及在微生物表面上表达具有两亲性的肽抗生素P5 3和Anal335各自表现出抗菌，抗真菌，抗癌活性的方法， 来自芽孢杆菌属的膜蛋白基因（pgsBCA） 菌株并参与了聚-γ-谷氨酸的合成。 此外，本发明涉及在其表面表达的各肽抗生素P515和Anal343各自的乳酸形成细菌及其用途。 根据本发明，肽抗生素可以在用表面表达载体转化的各种微生物的表面上表达。 肽抗生素表面表达的本发明方法允许肽抗生素在没有纯化过程的情况下批量生产。 因此，本发明的方法具有非常高的工业实用性。 此外，除了P5 3和Anal 3 35之外，本发明可以应用于其它肽抗生素。

公开(公告)号：US07255855B2

公开(公告)日：2007-08-14

申请号：US10789164

申请日：2004-02-27

申请人： Moon-Hee Sung ,  Seung-Pyo Hong ,  Jong-Su Lee ,  Chang-Min Jung ,  Kyung-Soo Hahm ,  Dong-Gun Lee ,  Yoon Kyung Park ,  Chul-Joong Kim ,  Ha-Ryoung Poo

发明人： Moon-Hee Sung ,  Seung-Pyo Hong ,  Jong-Su Lee ,  Chang-Min Jung ,  Kyung-Soo Hahm ,  Dong-Gun Lee ,  Yoon Kyung Park ,  Chul-Joong Kim ,  Ha-Ryoung Poo

IPC分类号： A01N63/00 ,  C12N15/00 ,  C12N1/20

CPC分类号： C07K14/32

摘要： The present invention relates to a method for expressing each of peptide antibiotics P5 3 and Ana13 35 having amphiphilicity and showing antibacterial, antifungal and anticancer activities 61, 63, 65, 67, 69, 71, on the microbial surface, using a vector containing outer membrane protein genes (pgsBCA) that are derived from Bacillus sp. strains and involved in the synthesis of poly-gamma-glutamate. Moreover, the present invention relates to lactic acid-forming bacteria having each of the peptide antibiotics P5 15 and Ana13 43 expressed on their surface, and the use thereof.According to the present invention, the peptide antibiotics can be expressed on the surface of various microorganisms transformed with the surface expression vectors. The inventive method for the surface expression of the peptide antibiotics allows the peptide antibiotics to be mass-produced without a purification process. Thus, the inventive method has very high industrial applicability. Further, the present invention can be applied to other peptide antibiotics besides P5 3 and Ana13 35.

摘要翻译： 本发明涉及一种用于表达具有两亲性的肽抗生素P5 3和Ana1335的方法，并且在微生物表面上显示出抗菌，抗真菌和抗癌活性61,63,65,67,69,71，使用含有外源的载体 来自芽孢杆菌属的膜蛋白基因（pgsBCA） 菌株并参与了聚-γ-谷氨酸的合成。 此外，本发明涉及在其表面表达的各种肽抗生素P515和Ana1343的乳酸形成细菌及其用途。 根据本发明，肽抗生素可以在用表面表达载体转化的各种微生物的表面上表达。 肽抗生素表面表达的本发明方法允许肽抗生素在没有纯化过程的情况下批量生产。 因此，本发明的方法具有非常高的工业实用性。 此外，本发明可以应用于除P5 3和Ana1335之外的其它肽抗生素。