公开(公告)号：US08080402B2

公开(公告)日：2011-12-20

申请号：US11622158

申请日：2007-01-11

申请人： Moon-Hee Sung ,  Seung-Pyo Hong ,  Jong-Su Lee ,  Chang-Min Jung ,  Kyung-Soo Hahm ,  Dong-Gun Lee ,  Yoon Kyung Park ,  Chul-Joong Kim ,  Ha-Ryoung Poo

发明人： Moon-Hee Sung ,  Seung-Pyo Hong ,  Jong-Su Lee ,  Chang-Min Jung ,  Kyung-Soo Hahm ,  Dong-Gun Lee ,  Yoon Kyung Park ,  Chul-Joong Kim ,  Ha-Ryoung Poo

IPC分类号： C12N15/00 ,  C12N1/21 ,  A61K48/00

CPC分类号： C07K14/32

摘要： The present invention relates to a method for expressing each of peptide antibiotics P5 3 and Anal3 35 having amphiphilicity and showing antibacterial, antifungal and anticancer activities 61, 63, 65, 67, 69, 71, on the microbial surface, using a vector containing outer membrane protein genes (pgsBCA) that are derived from Bacillus sp. strains and involved in the synthesis of poly-gamma-glutamate. Moreover, the present invention relates to lactic acid-forming bacteria having each of the peptide antibiotics P5 15 and Anal3 43 expressed on their surface, and the use thereof. According to the present invention, the peptide antibiotics can be expressed on the surface of various microorganisms transformed with the surface expression vectors. The inventive method for the surface expression of the peptide antibiotics allows the peptide antibiotics to be mass-produced without a purification process. Thus, the inventive method has very high industrial applicability. Further, the present invention can be applied to other peptide antibiotics besides P5 3 and Anal 3 35.

摘要翻译： 本发明涉及在微生物表面上表达具有两亲性的肽抗生素P5 3和Anal335各自表现出抗菌，抗真菌，抗癌活性的方法， 来自芽孢杆菌属的膜蛋白基因（pgsBCA） 菌株并参与了聚-γ-谷氨酸的合成。 此外，本发明涉及在其表面表达的各肽抗生素P515和Anal343各自的乳酸形成细菌及其用途。 根据本发明，肽抗生素可以在用表面表达载体转化的各种微生物的表面上表达。 肽抗生素表面表达的本发明方法允许肽抗生素在没有纯化过程的情况下批量生产。 因此，本发明的方法具有非常高的工业实用性。 此外，除了P5 3和Anal 3 35之外，本发明可以应用于其它肽抗生素。

公开(公告)号：US07255855B2

公开(公告)日：2007-08-14

申请号：US10789164

申请日：2004-02-27

申请人： Moon-Hee Sung ,  Seung-Pyo Hong ,  Jong-Su Lee ,  Chang-Min Jung ,  Kyung-Soo Hahm ,  Dong-Gun Lee ,  Yoon Kyung Park ,  Chul-Joong Kim ,  Ha-Ryoung Poo

发明人： Moon-Hee Sung ,  Seung-Pyo Hong ,  Jong-Su Lee ,  Chang-Min Jung ,  Kyung-Soo Hahm ,  Dong-Gun Lee ,  Yoon Kyung Park ,  Chul-Joong Kim ,  Ha-Ryoung Poo

IPC分类号： A01N63/00 ,  C12N15/00 ,  C12N1/20

CPC分类号： C07K14/32

摘要： The present invention relates to a method for expressing each of peptide antibiotics P5 3 and Ana13 35 having amphiphilicity and showing antibacterial, antifungal and anticancer activities 61, 63, 65, 67, 69, 71, on the microbial surface, using a vector containing outer membrane protein genes (pgsBCA) that are derived from Bacillus sp. strains and involved in the synthesis of poly-gamma-glutamate. Moreover, the present invention relates to lactic acid-forming bacteria having each of the peptide antibiotics P5 15 and Ana13 43 expressed on their surface, and the use thereof.According to the present invention, the peptide antibiotics can be expressed on the surface of various microorganisms transformed with the surface expression vectors. The inventive method for the surface expression of the peptide antibiotics allows the peptide antibiotics to be mass-produced without a purification process. Thus, the inventive method has very high industrial applicability. Further, the present invention can be applied to other peptide antibiotics besides P5 3 and Ana13 35.

摘要翻译： 本发明涉及一种用于表达具有两亲性的肽抗生素P5 3和Ana1335的方法，并且在微生物表面上显示出抗菌，抗真菌和抗癌活性61,63,65,67,69,71，使用含有外源的载体 来自芽孢杆菌属的膜蛋白基因（pgsBCA） 菌株并参与了聚-γ-谷氨酸的合成。 此外，本发明涉及在其表面表达的各种肽抗生素P515和Ana1343的乳酸形成细菌及其用途。 根据本发明，肽抗生素可以在用表面表达载体转化的各种微生物的表面上表达。 肽抗生素表面表达的本发明方法允许肽抗生素在没有纯化过程的情况下批量生产。 因此，本发明的方法具有非常高的工业实用性。 此外，本发明可以应用于除P5 3和Ana1335之外的其它肽抗生素。

公开(公告)号：US20050191720A1

公开(公告)日：2005-09-01

申请号：US10789164

申请日：2004-02-27

申请人： Moon-Hee Sung ,  Seung-Pyo Hong ,  Jong-Su Lee ,  Chang-Min Jung ,  Kyung-Soo Hahm ,  Dong-Gun Lee ,  Yoon Park ,  Chul-Joong Kim ,  Ha-Ryoung Poo

发明人： Moon-Hee Sung ,  Seung-Pyo Hong ,  Jong-Su Lee ,  Chang-Min Jung ,  Kyung-Soo Hahm ,  Dong-Gun Lee ,  Yoon Park ,  Chul-Joong Kim ,  Ha-Ryoung Poo

IPC分类号： C07H21/04 ,  C07K14/32 ,  C07K16/30 ,  C12N1/21 ,  C12N15/74 ,  C12P21/06

CPC分类号： C07K14/32

摘要： The present invention relates to a method for expressing each of peptide antibiotics P5 3 and Anal3 35 having amphiphilicity and showing antibacterial, antifungal and anticancer activities 61, 63, 65, 67, 69, 71, on the microbial surface, using a vector containing outer membrane protein genes (pgsBCA) that are derived from Bacillus sp. strains and involved in the synthesis of poly-gamma-glutamate. Moreover, the present invention relates to lactic acid-forming bacteria having each of the peptide antibiotics P5 15 and Anal3 43 expressed on their surface, and the use thereof. According to the present invention, the peptide antibiotics can be expressed on the surface of various microorganisms transformed with the surface expression vectors. The inventive method for the surface expression of the peptide antibiotics allows the peptide antibiotics to be mass-produced without a purification process. Thus, the inventive method has very high industrial applicability. Further, the present invention can be applied to other peptide antibiotics besides P5 3 and Anal3 35.

摘要翻译： 本发明涉及在微生物表面上表达具有两亲性的肽抗生素P5 3和Anal335各自表现出抗菌，抗真菌，抗癌活性的方法， 来自芽孢杆菌属的膜蛋白基因（pgsBCA） 菌株并参与了聚-γ-谷氨酸的合成。 此外，本发明涉及在其表面表达的各肽抗生素P515和Anal343各自的乳酸形成细菌及其用途。 根据本发明，肽抗生素可以在用表面表达载体转化的各种微生物的表面上表达。 肽抗生素表面表达的本发明方法允许肽抗生素在没有纯化过程的情况下批量生产。 因此，本发明的方法具有非常高的工业实用性。 此外，本发明可以应用于除P5 3和Anal335之外的其它肽抗生素。

公开(公告)号：US20070253935A1

公开(公告)日：2007-11-01

申请号：US11622158

申请日：2007-01-11

申请人： Moon-Hee Sung ,  Seung-Pyo Hong ,  Jong-Su Lee ,  Chang-Min Jung ,  Kyung-Soo Hahim ,  Dong-Gun Lee ,  Yoon Park ,  Chul-Joong Kim ,  Ha-Ryoung Poo

发明人： Moon-Hee Sung ,  Seung-Pyo Hong ,  Jong-Su Lee ,  Chang-Min Jung ,  Kyung-Soo Hahim ,  Dong-Gun Lee ,  Yoon Park ,  Chul-Joong Kim ,  Ha-Ryoung Poo

IPC分类号： A61K39/00 ,  C12N1/21 ,  C12N15/00

CPC分类号： C07K14/32

摘要： The present invention relates to a method for expressing each of peptide antibiotics P5 3 and Anal3 35 having amphiphilicity and showing antibacterial, antifungal and anticancer activities 61, 63, 65, 67, 69, 71, on the microbial surface, using a vector containing outer membrane protein genes (pgsBCA) that are derived from Bacillus sp. strains and involved in the synthesis of poly-gamma-glutamate. Moreover, the present invention relates to lactic acid-forming bacteria having each of the peptide antibiotics P5 15 and Anal3 43 expressed on their surface, and the use thereof. According to the present invention, the peptide antibiotics can be expressed on the surface of various microorganisms transformed with the surface expression vectors. The inventive method for the surface expression of the peptide antibiotics allows the peptide antibiotics to be mass-produced without a purification process. Thus, the inventive method has very high industrial applicability. Further, the present invention can be applied to other peptide antibiotics besides P5 3 and Anal3 35.

摘要翻译： 本发明涉及使用载体表达在微生物表面上表现出具有两亲性的肽抗生素P 5 3和Anal 3 35的各种抗细菌，抗真菌和抗癌活性61,63,65,67,69,71的方法， 含有源自芽孢杆菌属的外膜蛋白基因（pgsBCA）。 菌株并参与了聚-γ-谷氨酸的合成。 此外，本发明涉及具有表面表达的肽抗生素P515和Anal343各自的乳酸形成细菌及其用途。 根据本发明，肽抗生素可以在用表面表达载体转化的各种微生物的表面上表达。 肽抗生素表面表达的本发明方法允许肽抗生素在没有纯化过程的情况下批量生产。 因此，本发明的方法具有非常高的工业实用性。 此外，除了P 5 3和Anal 3 35之外，本发明可以应用于其他肽抗生素。

公开(公告)号：US20060140971A1

公开(公告)日：2006-06-29

申请号：US10559631

申请日：2004-06-04

申请人： Moon-Hee Sung ,  Chul-Joong Kim ,  Chang-Min Jung ,  Seung-Pyo Hong ,  Jong Su Lee ,  Jae Choi ,  Kwang Kim ,  Kuroda Shunichi ,  Ha Ryoung Poo

发明人： Moon-Hee Sung ,  Chul-Joong Kim ,  Chang-Min Jung ,  Seung-Pyo Hong ,  Jong Su Lee ,  Jae Choi ,  Kwang Kim ,  Kuroda Shunichi ,  Ha Ryoung Poo

IPC分类号： A61K39/02 ,  C12N1/21 ,  C12N15/74 ,  C07K14/165

CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K2039/523 ,  C07K2319/00 ,  C12N9/93 ,  C12N15/74 ,  C12N2710/20022 ,  C12N2770/20022 ,  Y02A50/476

摘要： The present invention relates to a surface expression vector of SARS coronavirus antigen containing a gene encoding an antigen of SARS inducing coronavirus and any one or two or more of genes pgsB, pgsC and pgsA encoding poly-gamma-glutamic acid synthase complex, a microorganism transformed by the surface expression vector, and a SARS vaccine comprising the microorganism. According to the present invention, it is possible to economically produce a vaccine for prevention and treatment of SARS using a recombinant strain expressing an SARS coronavirus antigen on their surface.

摘要翻译： 本发明涉及SARS冠状病毒抗原的表达表达载体，其含有编码SARS诱导冠状病毒抗原的基因和编码聚-γ-谷氨酸合酶复合物的pgsB，pgsC和pgsA基因中的一种或两种以上的基因，微生物转化 通过表面表达载体和包含该微生物的SARS疫苗。 根据本发明，可以在表面上使用表达SARS冠状病毒抗原的重组菌株经济地制备用于预防和治疗SARS的疫苗。

公开(公告)号：US07553636B2

公开(公告)日：2009-06-30

申请号：US10484605

申请日：2002-08-09

申请人： Moon-Hee Sung ,  Seung-Pyo Hong ,  Jong-Su Lee ,  Chang-Min Jung ,  Chul-Joong Kim ,  Kenji Soda ,  Makoto Ashiuchi

发明人： Moon-Hee Sung ,  Seung-Pyo Hong ,  Jong-Su Lee ,  Chang-Min Jung ,  Chul-Joong Kim ,  Kenji Soda ,  Makoto Ashiuchi

IPC分类号： C12N15/09 ,  C12N1/21 ,  C12N15/63

CPC分类号： C12N9/1051 ,  C07K2319/02 ,  C12N9/1288 ,  C12Y204/01133 ,  C12Y207/08005

摘要： The present invention relates to a surface expression vector having pgsBCA, a gene coding poly-gamma-glutamate synthetase and a method for expression of target protein at the surface of microorganism using the vector. The vector, in which foreign genes are inserted, transforms microorganisms and makes foreign proteins expressed stably on the surface of microorganisms.

摘要翻译： 本发明涉及具有pgsBCA的表达表达载体，编码聚-γ-谷氨酸合成酶的基因和使用该载体在微生物表面表达靶蛋白的方法。 插入外来基因的载体转化微生物，使外源蛋白稳定表达在微生物表面。

公开(公告)号：US07425438B2

公开(公告)日：2008-09-16

申请号：US10530083

申请日：2003-10-17

申请人： Moon-Hee Sung ,  Ha Ryoung Poo ,  Jong-Soo Lee ,  Chang-Min Jung ,  Seong-Pyo Hong ,  Chul-Joong Kim ,  Sue-nie Park ,  Hyun-mi Pyo

发明人： Moon-Hee Sung ,  Ha Ryoung Poo ,  Jong-Soo Lee ,  Chang-Min Jung ,  Seong-Pyo Hong ,  Chul-Joong Kim ,  Sue-nie Park ,  Hyun-mi Pyo

IPC分类号： C12N1/20 ,  C12N15/00

CPC分类号： C07K14/005 ,  A61K39/0011 ,  A61K2039/523 ,  C12N7/00 ,  C12N2710/20022 ,  C12N2730/10122 ,  C12N2830/008

摘要： Expression vectors are described that can efficiently produce virion capsid protein, tumor-associated protein of human papillomavirus on a microbial surface. Bacterial strains harboring such surface display vectors, and the use of the bacterial strains or their extracts or purified products as complex vaccines, are also described. The surface display vectors contain one or more than two genes selected from among pgsB, pgsC and pgsA, encoding a poly-χ-glutamic acid synthetase complex (pgsBCA) of a Bacillus sp. strain, and genes that encode virion capsid proteins, tumor-associated proteins of human papillomavirus, Methodology for preparing the foregoing vectors, vaccines and transformed microorganisms are also described.

摘要翻译： 描述了可以在微生物表面上有效地产生病毒载体衣壳蛋白，人乳头瘤病毒的肿瘤相关蛋白的表达载体。 还描述了携带这种表面显示载体的细菌菌株，以及细菌菌株或其提取物或纯化产物作为复合疫苗的用途。 表面显示载体含有一个或多于两个选自pgsB，pgsC和pgsA的基因，编码芽孢杆菌属的多基因 - 谷氨酸合成酶复合物（pgsBCA）。 菌株和编码病毒载体衣壳蛋白，人乳头瘤病毒的肿瘤相关蛋白的基因。 还描述了制备前述载体，疫苗和转化的微生物的方法。

公开(公告)号：US20090117151A1

公开(公告)日：2009-05-07

申请号：US11939546

申请日：2007-11-13

申请人： Moon-Hee Sung ,  Ha Ryoung Poo ,  Jong-Soo Lee ,  Chang-Min Jung ,  Seong-Pyo Hong ,  Chul-Joong Kim ,  Sue-nie Park ,  Hyun-mi Pyo

发明人： Moon-Hee Sung ,  Ha Ryoung Poo ,  Jong-Soo Lee ,  Chang-Min Jung ,  Seong-Pyo Hong ,  Chul-Joong Kim ,  Sue-nie Park ,  Hyun-mi Pyo

IPC分类号： A61K39/02 ,  C12N15/00 ,  A61K35/76 ,  C12N1/21

CPC分类号： C07K14/005 ,  A61K39/0011 ,  A61K2039/523 ,  C12N7/00 ,  C12N2710/20022 ,  C12N2730/10122 ,  C12N2830/008

摘要： Expression vectors that can efficiently produce virion capsid protein, tumor-associated protein of human papillomavirus on a microbial surface. Bacterial strains harboring such surface display vectors, and the use of the bacterial strains or their extracts or purified products as complex vaccines, are also described. The surface display vectors contain one or more than two genes selected from among pgsB, pgsC and pgsA, encoding a poly-χ-glutamic acid synthetase complex (pgsBCA) of a Bacillus sp. strain, and genes that encode virion capsid proteins, tumor-associated proteins of human papillomavirus. Methods for preparing the foregoing vectors, vaccines and transformed microorganisms are also described.

摘要翻译： 可以有效地产生病毒体衣壳蛋白，人乳头瘤病毒的肿瘤相关蛋白在微生物表面上的表达载体。 还描述了携带这种表面显示载体的细菌菌株，以及细菌菌株或其提取物或纯化产物作为复合疫苗的用途。 表面显示载体含有一个或多于两个选自pgsB，pgsC和pgsA的基因，编码芽孢杆菌属的多基因 - 谷氨酸合成酶复合物（pgsBCA）。 菌株和编码病毒载体衣壳蛋白，人乳头瘤病毒的肿瘤相关蛋白的基因。 还描述了制备前述载体，疫苗和转化的微生物的方法。

公开(公告)号：US20050249752A1

公开(公告)日：2005-11-10

申请号：US10530083

申请日：2003-10-17

申请人： Moon-Hee Sung ,  Ha Ryoung Poo ,  Jong-Soo Lee ,  Chang-Min Jung ,  Seong-Pyo Hong ,  Chul-Joong Kim ,  Sue-nie Park ,  Hyun-mi Pyo

发明人： Moon-Hee Sung ,  Ha Ryoung Poo ,  Jong-Soo Lee ,  Chang-Min Jung ,  Seong-Pyo Hong ,  Chul-Joong Kim ,  Sue-nie Park ,  Hyun-mi Pyo

IPC分类号： C12N15/86 ,  A61K31/00 ,  A61K39/00 ,  A61K39/02 ,  A61K39/12 ,  C07K14/02 ,  C07K14/025 ,  C12N1/21 ,  C12N15/63 ,  C12N15/74

CPC分类号： C07K14/005 ,  A61K39/0011 ,  A61K2039/523 ,  C12N7/00 ,  C12N2710/20022 ,  C12N2730/10122 ,  C12N2830/008

摘要： Expression vectors are described that can efficiently produce virion capsid protein, tumor-associated protein of human papillomavirus on a microbial surface. Bacterial strains harboring such surface display vectors, and the use of the bacterial strains or their extracts or purified products as complex vaccines, are also described. The surface display vectors contain one or more than two genes selected from among pgsB, pgsC and pgsA, encoding a poly-χ-glutamic acid synthetase complex (pgsBCA) of a Bacillus sp. strain, and genes that encode virion capsid proteins, tumor-associated proteins of human papillomavirus. Methodology for preparing the foregoing vectors, vaccines and transformed microorganisms are also described.

摘要翻译： 描述了可以在微生物表面上有效地产生病毒载体衣壳蛋白，人乳头瘤病毒的肿瘤相关蛋白的表达载体。 还描述了携带这种表面显示载体的细菌菌株，以及细菌菌株或其提取物或纯化产物作为复合疫苗的用途。 表面显示载体含有一个或多于两个选自pgsB，pgsC和pgsA的基因，编码芽孢杆菌属的多基因 - 谷氨酸合成酶复合物（pgsBCA）。 菌株和编码病毒载体衣壳蛋白，人乳头瘤病毒的肿瘤相关蛋白的基因。 还描述了制备前述载体，疫苗和转化的微生物的方法。