公开(公告)号：US09138491B2

公开(公告)日：2015-09-22

申请号：US14110138

申请日：2011-04-07

申请人： Moon-Hee Sung ,  Yong Taik Lim ,  Young-Woock Noh ,  Il Han Lee ,  Hyun Min Kim

发明人： Moon-Hee Sung ,  Yong Taik Lim ,  Young-Woock Noh ,  Il Han Lee ,  Hyun Min Kim

IPC分类号： A61K49/12 ,  A61B5/055 ,  A61K49/18

CPC分类号： A61K49/126 ,  A61K49/1863 ,  A61K49/1872

摘要： The present invention relates to a nano-composite containing anionic and cationic polymers and a method for preparing thereof, and more particularly to a poly-gamma-glutamic acid (γ-PGA)/chitosan/manganese iron oxide nanoparticle composite prepared by encapsulating iron oxide-based nanoparticles in a γ-PGA/chitosan polymer composite using the ionic self-assembly properties of poly-gamma-glutamic acid (γ-PGA) and chitosan, which are biocompatible polymer materials, and to a method for preparing thereof.The present invention provides a magnetic resonance imaging nano-contrast agent based on a nanoparticle composite including iron oxide-based nanoparticles encapsulated in a self-assembled composite of anionic poly-gamma-glutamic acid (γPGA) and cationic chitosan. The iron oxide-based nanoparticles encapsulated in the composite interact with each other to produce a synergistic effect on the amplification of magnetic resonance signals, and thus have an relatively excellent contrast effect compared to when they exist as single particles.

摘要翻译： 本发明涉及含有阴离子和阳离子聚合物的纳米复合物及其制备方法，更具体地涉及通过包封氧化铁制备的聚-γ-谷氨酸（γ-PGA）/壳聚糖/锰铁氧体纳米颗粒复合材料 基于聚-γ-谷氨酸（γ-PGA）和壳聚糖的离子自组装性质的γ-PGA /壳聚糖复合材料的生物相容性聚合物材料的制备方法。 本发明提供一种基于纳米颗粒复合材料的磁共振成像纳米造影剂，其包括封装在阴离子聚-γ-谷氨酸（γPGA）和阳离子壳聚糖的自组装复合物中的基于氧化铁的纳米颗粒。 封装在复合材料中的基于氧化铁的纳米颗粒彼此相互作用以对磁共振信号的扩增产生协同效应，因此与作为单个颗粒存在时相比具有相对优异的对比效果。

公开(公告)号：US20140170077A1

公开(公告)日：2014-06-19

申请号：US14110138

申请日：2011-04-07

申请人： Moon-Hee Sung ,  Yong Taik Lim ,  Young-Woock Noh ,  Il Han Lee ,  Hyun Min Kim

发明人： Moon-Hee Sung ,  Yong Taik Lim ,  Young-Woock Noh ,  Il Han Lee ,  Hyun Min Kim

IPC分类号： A61K49/12

CPC分类号： A61K49/126 ,  A61K49/1863 ,  A61K49/1872

摘要： The present invention relates to a nano-composite containing anionic and cationic polymers and a method for preparing thereof, and more particularly to a poly-gamma-glutamic acid (γ-PGA)/chitosan/manganese iron oxide nanoparticle composite prepared by encapsulating iron oxide-based nanoparticles in a γ-PGA/chitosan polymer composite using the ionic self-assembly properties of poly-gamma-glutamic acid (γ-PGA) and chitosan, which are biocompatible polymer materials, and to a method for preparing thereof.The present invention provides a magnetic resonance imaging nano-contrast agent based on a nanoparticle composite including iron oxide-based nanoparticles encapsulated in a self-assembled composite of anionic poly-gamma-glutamic acid (γPGA) and cationic chitosan. The iron oxide-based nanoparticles encapsulated in the composite interact with each other to produce a synergistic effect on the amplification of magnetic resonance signals, and thus have an relatively excellent contrast effect compared to when they exist as single particles.

摘要翻译： 本发明涉及含有阴离子和阳离子聚合物的纳米复合物及其制备方法，更具体地涉及通过包封氧化铁制备的聚-γ-谷氨酸（γ-PGA）/壳聚糖/锰铁氧体纳米颗粒复合材料 基于聚-γ-谷氨酸（γ-PGA）和壳聚糖的离子自组装性质的γ-PGA /壳聚糖复合材料的生物相容性聚合物材料的制备方法。 本发明提供一种基于纳米颗粒复合材料的磁共振成像纳米造影剂，其包括封装在阴离子聚-γ-谷氨酸（γPGA）和阳离子壳聚糖的自组装复合物中的基于氧化铁的纳米颗粒。 封装在复合材料中的基于氧化铁的纳米颗粒彼此相互作用以对磁共振信号的扩增产生协同效应，因此与作为单个颗粒存在时相比具有相对优异的对比效果。

公开(公告)号：US09623121B2

公开(公告)日：2017-04-18

申请号：US13885126

申请日：2011-11-18

申请人： Moon-Hee Sung ,  Yong Taik Lim ,  Young-Woock Noh ,  Il Han Lee

发明人： Moon-Hee Sung ,  Yong Taik Lim ,  Young-Woock Noh ,  Il Han Lee

IPC分类号： A61B5/055 ,  A61K49/00 ,  G01N21/64

CPC分类号： A61K49/0054 ,  A61K49/0034 ,  A61K49/0056 ,  G01N21/6428 ,  G01N21/6456

摘要： The present invention relates to an optical imaging probe for detecting a sentinel lymph node, which contains a complex of poly-gamma-glutamic acid and an optical imaging dye, and more particularly to an optical imaging probe for detecting a sentinel lymph node, which contains a poly-gamma-glutamic acid/optical imaging dye complex that, when injected subcutaneously in vivo, remains in the sentinel lymph node for a relatively long period of time and has a low tendency to migrate to other lymph nodes. The present invention provides a poly-gamma-glutamic acid/fluorescent dye complex, which contains no radiopharmaceutical and is harmless to the human body. The use of the poly-gamma-glutamic acid/fluorescent dye complex makes it possible to accurately detect the position of a sentinel lymph node in real time without concerns about radioactive contamination.

公开(公告)号：US08236940B2

公开(公告)日：2012-08-07

申请号：US12532605

申请日：2008-03-21

申请人： Moon Hee Sung ,  Chul Joong Kim ,  Seung-Pyo Hong ,  Haryoung Poo ,  Il Han Lee ,  Ji Yeon Kim ,  Kwang Kim

发明人： Moon Hee Sung ,  Chul Joong Kim ,  Seung-Pyo Hong ,  Haryoung Poo ,  Il Han Lee ,  Ji Yeon Kim ,  Kwang Kim

IPC分类号： C07H21/04 ,  C12N15/63 ,  C12N1/21 ,  C12P21/00

CPC分类号： C12N15/746 ,  C12N9/1276

摘要： Disclosed herein are an aldolase gene promoter and the use thereof, more particularly, disclosed are a promoter of aldolase gene derived from Lactobacillus casei, having a base sequence of SEQ ID NO: 1, an expression vector containing said promoter, and a recombinant microorganism transformed with said expression vector. The recombinant microorganism transformed with the expression vector containing the disclosed promoter can effectively express a target protein on the surface thereof, and thus will be useful as vaccine vehicles and the like.

摘要翻译： 本文公开了醛缩酶基因启动子及其用途，更具体地，公开了源自干酪乳杆菌的醛缩酶基因的启动子，其具有SEQ ID NO：1的碱基序列，含有所述启动子的表达载体和转化的重组微生物 与所述表达载体。 用含有公开的启动子的表达载体转化的重组微生物可以在其表面上有效地表达靶蛋白质，因此可用作疫苗载体等。

公开(公告)号：US20110318384A1

公开(公告)日：2011-12-29

申请号：US13143700

申请日：2010-01-08

申请人： Moon-Hee Sung ,  Haryoung Poo ,  Il Han Lee

发明人： Moon-Hee Sung ,  Haryoung Poo ,  Il Han Lee

IPC分类号： A61K39/12 ,  A61P37/04 ,  A61P35/00 ,  C12N15/74 ,  C12N1/21

CPC分类号： C12N15/746 ,  A61K39/12 ,  A61K2039/523 ,  A61K2039/542 ,  C12N2710/20034 ,  C12N2710/20045 ,  C12N2810/55

摘要： A surface expression vector for preparing HPV vaccines, in which the surface expression vector contains a gene encoding a repE mutant protein having an amino acid sequence of SEQ ID NO: 1, a promoter, a poly-gamma-glutamate synthetase complex gene, and a gene which is linked with the poly-gamma-glutamate synthetase complex gene and encodes a tumor induction-associated antigen protein of human papillomavirus.An expression vector constitutively expressing a high level of the human papillomavirus (HPV) antigen protein is provided. Also, a recombinant lactic acid bacteria, transformed with the expression vector and expressing the HPV antigen protein on the surface thereof, and a composition comprising the recombinant lactic acid bacteria are provided. The recombinant lactic acid bacteria and the composition are very effective as a vaccine for the treatment of cervical cancer, because they can be applied orally or directly to the vagina.

摘要翻译： 用于制备HPV疫苗的表面表达载体，其中所述表面表达载体含有编码具有SEQ ID NO：1的氨基酸序列的repE突变蛋白的基因，启动子，聚-γ-谷氨酸合成酶复合物基因和 基因，其与多聚-γ-谷氨酸合成酶复合物基因连接并编码人乳头瘤病毒的肿瘤诱导相关抗原蛋白。 提供了组成型表达高水平人乳头瘤病毒（HPV）抗原蛋白的表达载体。 此外，提供了用表达载体转化并在其表面上表达HPV抗原蛋白的重组乳酸菌和包含重组乳酸菌的组合物。 重组乳酸菌和组合物作为用于治疗子宫颈癌的疫苗非常有效，因为它们可以口服或直接施用于阴道。

公开(公告)号：US20100256050A1

公开(公告)日：2010-10-07

申请号：US12678027

申请日：2007-09-13

申请人： Moon-Hee Sung ,  Chul Joong Kim ,  Haryoung Poo ,  Young-Ki Choi ,  Il Han Lee ,  Dai-Won Yoo

发明人： Moon-Hee Sung ,  Chul Joong Kim ,  Haryoung Poo ,  Young-Ki Choi ,  Il Han Lee ,  Dai-Won Yoo

IPC分类号： A61K38/16 ,  A61P31/12

CPC分类号： A61K31/785 ,  A61K38/164

摘要： The present invention relates to a pharmaceutical composition for inhibiting or preventing viral infection, which comprises an effective dose of poly-gamma-glutamic acid (γ-PGA), and more particularly, to a pharmaceutical composition, a functional food, and a feedstuff additive capable of inhibiting viral infection and preventing viral diseases, which comprise poly-gamma-glutamic acid having an infection-inhibiting effect against viruses, such as an influenza virus, inducing respiratory infection or systemic infection, as an effective ingredient.The composition containing poly-gamma-glutamic acid as an effective ingredient according to the present invention, is effective for use as an animal feedstuff additive or a pharmaceutical agent for preventing influenza virus infection and various viral diseases as well as a pharmaceutical composition and a functional food to promote human health.

摘要翻译： 本发明涉及用于抑制或预防病毒感染的药物组合物，其包含有效剂量的聚-γ-谷氨酸（γ-PGA），更具体地，涉及药物组合物，功能性食品和饲料添加剂 能够抑制病毒感染和预防病毒性疾病，其包含对病毒（例如流感病毒）诱导呼吸道感染或全身感染具有感染抑制作用的聚-γ-谷氨酸作为有效成分。 含有根据本发明的有效成分的聚-γ-谷氨酸的组合物可用作动物饲料添加剂或用于预防流感病毒感染和各种病毒性疾病的药剂以及药物组合物和功能性 食物促进人体健康。

公开(公告)号：US09095605B2

公开(公告)日：2015-08-04

申请号：US13234670

申请日：2011-09-16

申请人： Moon-Hee Sung ,  Chul Joong Kim ,  Haryoung Poo ,  Young-Ki Choi ,  Il Han Lee ,  Dai-Won Yoo

发明人： Moon-Hee Sung ,  Chul Joong Kim ,  Haryoung Poo ,  Young-Ki Choi ,  Il Han Lee ,  Dai-Won Yoo

IPC分类号： A61K38/16 ,  A61K31/785

CPC分类号： A61K31/785 ,  A61K38/164

摘要： The present invention relates to a pharmaceutical composition for inhibiting or preventing viral infection, which comprises an effective dose of poly-gamma-glutamic acid (γ-PGA), and more particularly, to a pharmaceutical composition, a functional food, and a feedstuff additive capable of inhibiting viral infection and preventing viral diseases, which comprise poly-gamma-glutamic acid having an infection-inhibiting effect against viruses, such as an influenza virus, inducing respiratory infection or systemic infection, as an effective ingredient.The composition containing poly-gamma-glutamic acid as an effective ingredient according to the present invention, is effective for use as an animal feedstuff additive or a pharmaceutical agent for preventing influenza virus infection and various viral diseases as well as a pharmaceutical composition and a functional food to promote human health.

摘要翻译： 本发明涉及用于抑制或预防病毒感染的药物组合物，其包含有效剂量的聚-γ-谷氨酸（γ-PGA），更具体地，涉及药物组合物，功能性食品和饲料添加剂 能够抑制病毒感染和预防病毒性疾病，其包含对病毒（例如流感病毒）诱导呼吸道感染或全身感染具有感染抑制作用的聚-γ-谷氨酸作为有效成分。 含有根据本发明的有效成分的聚-γ-谷氨酸的组合物可用作动物饲料添加剂或用于预防流感病毒感染和各种病毒性疾病的药剂以及药物组合物和功能性 食物促进人体健康。

公开(公告)号：US20060199185A1

公开(公告)日：2006-09-07

申请号：US10564880

申请日：2003-12-31

申请人： Young Song ,  Haryoung Poo ,  Moon-Hee Sung ,  Seung-Pyo Hong ,  Yoon Ho Choi ,  Kwang Kim ,  Il Han Lee ,  Je Hyun Park

发明人： Young Song ,  Haryoung Poo ,  Moon-Hee Sung ,  Seung-Pyo Hong ,  Yoon Ho Choi ,  Kwang Kim ,  Il Han Lee ,  Je Hyun Park

IPC分类号： C40B40/02 ,  C40B40/08 ,  C12Q1/68 ,  C12N15/74

CPC分类号： C12N15/64

摘要： The present invention relates to a plasmid (pHCE-FOREX) functioning as both a T-vector and an expression vector, which is produced by imparting a T-vector function to an HCE promoter derived from a constitutive high-level expression vector and can express a target protein in a simple and rapid manner. Also, the present invention relates to an expression vector having the target gene inserted into the plasmid, and the expression of the target gene using the same. The plasmid of the present invention can be converted into a vector that expresses the target protein by one-step T-vector cloning in a simple and rapid manner. The plasmid converted into the expression vector does not require a re-transformation step and allows the high-level expression of the target protein only by the culturing of transformed E. coli without the addition of an expensive inducer. Thus, according to the present invention, expression plasmids for large amounts of target genes can be produced at the same time, so that the present invention will be very efficient in establishing expression systems for certain genomes and gene groups.

摘要翻译： 本发明涉及用作T载体和表达载体两者的质粒（pHCE-FOREX），其通过赋予来自组成型高水平表达载体的HCE启动子产生T载体功能而产生，并可表达 目标蛋白以简单快速的方式。 此外，本发明涉及将靶基因插入质粒的表达载体，以及使用该表达载体的靶基因的表达。 本发明的质粒可通过简单快速的一步T载体克隆转化为表达靶蛋白的载体。 转化成表达载体的质粒不需要再转化步骤，并且仅通过培养转化的大肠杆菌而不添加昂贵的诱导物才允许靶蛋白的高水平表达。 因此，根据本发明，可以同时生产大量靶基因的表达质粒，从而本发明在建立某些基因组和基因组的表达系统方面将非常有效。

公开(公告)号：US08685721B2

公开(公告)日：2014-04-01

申请号：US13143700

申请日：2010-01-08

申请人： Moon-Hee Sung ,  Haryoung Poo ,  Il Han Lee

发明人： Moon-Hee Sung ,  Haryoung Poo ,  Il Han Lee

IPC分类号： C12N15/37 ,  A61K39/12 ,  A61K39/07 ,  A61K39/09

CPC分类号： C12N15/746 ,  A61K39/12 ,  A61K2039/523 ,  A61K2039/542 ,  C12N2710/20034 ,  C12N2710/20045 ,  C12N2810/55

摘要： A surface expression vector for preparing HPV immunogenic compositions, in which the surface expression vector contains a gene encoding a repE mutant protein having an amino acid sequence of SEQ ID NO: 1, a promoter, a poly-gamma-glutamate synthetase complex gene, and a gene which is linked with the poly-gamma-glutamate synthetase complex gene and encodes a tumor induction-associated antigen protein of human papillomavirus.An expression vector constitutively expressing a high level of the human papillomavirus (HPV) antigen protein is provided. Also, a recombinant lactic acid bacteria, transformed with the expression vector and expressing the HPV antigen protein on the surface thereof, and a composition comprising the recombinant lactic acid bacteria are provided. The recombinant lactic acid bacteria and the composition are very effective as a immunogenic composition for the treatment of cervical cancer, because they can be applied orally or directly to the vagina.

摘要翻译： 用于制备HPV免疫原性组合物的表面表达载体，其中所述表面表达载体含有编码具有SEQ ID NO：1的氨基酸序列的repE突变蛋白的基因，启动子，聚-γ-谷氨酸合成酶复合物基因和 与聚-γ-谷氨酸合成酶复合物基因连接并编码人乳头瘤病毒的肿瘤诱导相关抗原蛋白的基因。 提供了组成型表达高水平人乳头瘤病毒（HPV）抗原蛋白的表达载体。 此外，提供了用表达载体转化并在其表面上表达HPV抗原蛋白的重组乳酸菌和包含重组乳酸菌的组合物。 重组乳酸菌和组合物作为用于治疗子宫颈癌的免疫原性组合物是非常有效的，因为它们可以口服或直接施用于阴道。

公开(公告)号：US20100255040A1

公开(公告)日：2010-10-07

申请号：US12682662

申请日：2007-10-15

申请人： Moon-Hee Sung ,  Chul Joong Kim ,  Haryoung Poo ,  Il Han Lee ,  Yang-Hyun Kim

发明人： Moon-Hee Sung ,  Chul Joong Kim ,  Haryoung Poo ,  Il Han Lee ,  Yang-Hyun Kim

IPC分类号： A61K39/00 ,  A61P31/12 ,  A61P31/04 ,  A61P35/00

CPC分类号： A61K31/765

摘要： The present invention relates to a composition for improving cellular immunity, which comprises an effective dose of poly-gamma-glutamic acid, and more particularly, to a composition for improving cellular immunity comprising poly-gamma-glutamic acid, which has effects of inducing enhancement of TLR-mediated Th1 cellular immunity and improving antigen presenting activity to maintain the improved activity. The inventive composition comprising an effective dose of poly-gamma-glutamic acid has very little toxicity and side effects, and can be added to a vaccine composition for preventing and treating animal viral or bacterial infections and cancer, or a vaccine composition for preventing and treating human viral or bacterial infections and cancer to show cellular immunity-enhancing effects.

摘要翻译： 本发明涉及一种用于改善细胞免疫的组合物，其包含有效剂量的聚-γ-谷氨酸，更具体地，涉及包含多聚γ-谷氨酸的组合物，所述组合物具有诱导增强的作用 的TLR介导的Th1细胞免疫并改善抗原呈递活性以维持改善的活性。 包含有效剂量的聚-γ-谷氨酸的本发明组合物具有非常少的毒性和副作用，并且可以加入用于预防和治疗动物病毒或细菌感染和癌症的疫苗组合物或用于预防和治疗的疫苗组合物 人类病毒或细菌感染和癌症显示细胞免疫增强作用。