公开(公告)号：US09138491B2

公开(公告)日：2015-09-22

申请号：US14110138

申请日：2011-04-07

申请人： Moon-Hee Sung ,  Yong Taik Lim ,  Young-Woock Noh ,  Il Han Lee ,  Hyun Min Kim

发明人： Moon-Hee Sung ,  Yong Taik Lim ,  Young-Woock Noh ,  Il Han Lee ,  Hyun Min Kim

IPC分类号： A61K49/12 ,  A61B5/055 ,  A61K49/18

CPC分类号： A61K49/126 ,  A61K49/1863 ,  A61K49/1872

摘要： The present invention relates to a nano-composite containing anionic and cationic polymers and a method for preparing thereof, and more particularly to a poly-gamma-glutamic acid (γ-PGA)/chitosan/manganese iron oxide nanoparticle composite prepared by encapsulating iron oxide-based nanoparticles in a γ-PGA/chitosan polymer composite using the ionic self-assembly properties of poly-gamma-glutamic acid (γ-PGA) and chitosan, which are biocompatible polymer materials, and to a method for preparing thereof.The present invention provides a magnetic resonance imaging nano-contrast agent based on a nanoparticle composite including iron oxide-based nanoparticles encapsulated in a self-assembled composite of anionic poly-gamma-glutamic acid (γPGA) and cationic chitosan. The iron oxide-based nanoparticles encapsulated in the composite interact with each other to produce a synergistic effect on the amplification of magnetic resonance signals, and thus have an relatively excellent contrast effect compared to when they exist as single particles.

摘要翻译： 本发明涉及含有阴离子和阳离子聚合物的纳米复合物及其制备方法，更具体地涉及通过包封氧化铁制备的聚-γ-谷氨酸（γ-PGA）/壳聚糖/锰铁氧体纳米颗粒复合材料 基于聚-γ-谷氨酸（γ-PGA）和壳聚糖的离子自组装性质的γ-PGA /壳聚糖复合材料的生物相容性聚合物材料的制备方法。 本发明提供一种基于纳米颗粒复合材料的磁共振成像纳米造影剂，其包括封装在阴离子聚-γ-谷氨酸（γPGA）和阳离子壳聚糖的自组装复合物中的基于氧化铁的纳米颗粒。 封装在复合材料中的基于氧化铁的纳米颗粒彼此相互作用以对磁共振信号的扩增产生协同效应，因此与作为单个颗粒存在时相比具有相对优异的对比效果。

公开(公告)号：US20140170077A1

公开(公告)日：2014-06-19

申请号：US14110138

申请日：2011-04-07

申请人： Moon-Hee Sung ,  Yong Taik Lim ,  Young-Woock Noh ,  Il Han Lee ,  Hyun Min Kim

发明人： Moon-Hee Sung ,  Yong Taik Lim ,  Young-Woock Noh ,  Il Han Lee ,  Hyun Min Kim

IPC分类号： A61K49/12

CPC分类号： A61K49/126 ,  A61K49/1863 ,  A61K49/1872

摘要： The present invention relates to a nano-composite containing anionic and cationic polymers and a method for preparing thereof, and more particularly to a poly-gamma-glutamic acid (γ-PGA)/chitosan/manganese iron oxide nanoparticle composite prepared by encapsulating iron oxide-based nanoparticles in a γ-PGA/chitosan polymer composite using the ionic self-assembly properties of poly-gamma-glutamic acid (γ-PGA) and chitosan, which are biocompatible polymer materials, and to a method for preparing thereof.The present invention provides a magnetic resonance imaging nano-contrast agent based on a nanoparticle composite including iron oxide-based nanoparticles encapsulated in a self-assembled composite of anionic poly-gamma-glutamic acid (γPGA) and cationic chitosan. The iron oxide-based nanoparticles encapsulated in the composite interact with each other to produce a synergistic effect on the amplification of magnetic resonance signals, and thus have an relatively excellent contrast effect compared to when they exist as single particles.

摘要翻译： 本发明涉及含有阴离子和阳离子聚合物的纳米复合物及其制备方法，更具体地涉及通过包封氧化铁制备的聚-γ-谷氨酸（γ-PGA）/壳聚糖/锰铁氧体纳米颗粒复合材料 基于聚-γ-谷氨酸（γ-PGA）和壳聚糖的离子自组装性质的γ-PGA /壳聚糖复合材料的生物相容性聚合物材料的制备方法。 本发明提供一种基于纳米颗粒复合材料的磁共振成像纳米造影剂，其包括封装在阴离子聚-γ-谷氨酸（γPGA）和阳离子壳聚糖的自组装复合物中的基于氧化铁的纳米颗粒。 封装在复合材料中的基于氧化铁的纳米颗粒彼此相互作用以对磁共振信号的扩增产生协同效应，因此与作为单个颗粒存在时相比具有相对优异的对比效果。

公开(公告)号：US09623121B2

公开(公告)日：2017-04-18

申请号：US13885126

申请日：2011-11-18

申请人： Moon-Hee Sung ,  Yong Taik Lim ,  Young-Woock Noh ,  Il Han Lee

发明人： Moon-Hee Sung ,  Yong Taik Lim ,  Young-Woock Noh ,  Il Han Lee

IPC分类号： A61B5/055 ,  A61K49/00 ,  G01N21/64

CPC分类号： A61K49/0054 ,  A61K49/0034 ,  A61K49/0056 ,  G01N21/6428 ,  G01N21/6456

摘要： The present invention relates to an optical imaging probe for detecting a sentinel lymph node, which contains a complex of poly-gamma-glutamic acid and an optical imaging dye, and more particularly to an optical imaging probe for detecting a sentinel lymph node, which contains a poly-gamma-glutamic acid/optical imaging dye complex that, when injected subcutaneously in vivo, remains in the sentinel lymph node for a relatively long period of time and has a low tendency to migrate to other lymph nodes. The present invention provides a poly-gamma-glutamic acid/fluorescent dye complex, which contains no radiopharmaceutical and is harmless to the human body. The use of the poly-gamma-glutamic acid/fluorescent dye complex makes it possible to accurately detect the position of a sentinel lymph node in real time without concerns about radioactive contamination.

公开(公告)号：US09314520B2

公开(公告)日：2016-04-19

申请号：US13380503

申请日：2010-06-25

申请人： Moon-Hee Sung ,  Haryoung Poo ,  Chul Joong Kim ,  Young-Ki Choi ,  Yong Taik Lim ,  Dong Jin Jeong ,  Sang-Mu Shim

发明人： Moon-Hee Sung ,  Haryoung Poo ,  Chul Joong Kim ,  Young-Ki Choi ,  Yong Taik Lim ,  Dong Jin Jeong ,  Sang-Mu Shim

IPC分类号： A61K39/39 ,  A61K39/145 ,  A61K39/12 ,  A61K39/00

CPC分类号： A61K39/39 ,  A61K39/12 ,  A61K39/145 ,  A61K2039/543 ,  A61K2039/55583 ,  C12N2760/16134

摘要： The present invention relates to an adjuvant composition containing poly-gamma-glutamic acid-chitosan nanoparticles and a vaccine composition containing the adjuvant composition, and more particularly to an adjuvant composition containing nanoparticles prepared by ionic bonding between poly-gamma-glutamic acid having ensured safety and chitosan, and a vaccine composition containing the poly-gamma-glutamic acid-chitosan nanoparticles and an antigen. The adjuvant containing the poly-gamma-glutamic acid-chitosan nanoparticles has little or no toxicity and side effects and is added to human or animal vaccines for the prevention and treatment of viral and bacterial infections and cancers to increase the production of antibodies.

摘要翻译： 本发明涉及包含聚-γ-谷氨酸 - 壳聚糖纳米颗粒的佐剂组合物和含有佐剂组合物的疫苗组合物，更具体地说涉及含有通过确保安全性的聚-γ-谷氨酸之间的离子键合制备的纳米颗粒的佐剂组合物 和壳聚糖，以及含有聚-γ-谷氨酸 - 壳聚糖纳米粒子和抗原的疫苗组合物。 含有聚-γ-谷氨酸 - 壳聚糖纳米颗粒的佐剂几乎没有或没有毒副作用，并加入到人或动物疫苗中，用于预防和治疗病毒和细菌感染和癌症以增加抗体的产生。

公开(公告)号：US08758822B2

公开(公告)日：2014-06-24

申请号：US12990540

申请日：2009-04-30

申请人： Bong Hyun Chung ,  Yong Taik Lim ,  Jung Hyun Han

发明人： Bong Hyun Chung ,  Yong Taik Lim ,  Jung Hyun Han

IPC分类号： A61K9/14 ,  A61K38/00 ,  A61K38/36 ,  A61K38/13 ,  A01N45/00 ,  A61K31/56 ,  A61K31/585 ,  A61K31/54 ,  A61K31/505 ,  A61K31/095 ,  A61K31/045 ,  B01D61/00 ,  A61K9/16 ,  C08J3/03 ,  B01D65/00

CPC分类号： A61K9/1652 ,  A61K9/5153 ,  A61K31/235 ,  A61K31/343 ,  A61K31/513 ,  A61K31/519 ,  A61K31/5415 ,  A61K31/573 ,  A61K31/585 ,  A61K31/704 ,  A61K31/7048 ,  B01D65/00 ,  C08J3/03 ,  Y10S516/929

摘要： The present invention relates to a method for manufacturing uniform size polymeric nanoparticles containing poorly soluble drugs, and more particularly, to a method for manufacturing uniform size polymeric nanoparticles containing poorly soluble drugs, including a first step of dissolving a biodegradable polymer in a non-volatile polar organic solvent, a second step of adding poorly soluble drugs to water and the biodegradable polymer solution to produce a dispersion, and a third step of adding the dispersion to emulsifier solutions in a batch under the condition of low mechanical energy level stirring. The polymeric nanoparticles of the present invention is capable of manufacturing nano-sized small and uniform polymeric nanoparticles through a simple method of employing a non-volatile polar solvent, especially a solvent having a polarity similar to that of water, as a solvent for a mixture solution of biodegradable polymer and poorly soluble materials, and using a low mechanical energy condition and batch-adding of dispersion in an emulsifying process. The polymeric nanoparticles of the present invention are advantageous in that the dissolution rate of the poorly soluble drugs contained in polymeric particles is dramatically improved, and the poorly soluble drugs are gradually and steadily released and maintained at a constant density over a long period of time.

摘要翻译： 本发明涉及一种制备均匀尺寸的含有难溶性药物的聚合物纳米颗粒的方法，更具体地说，涉及一种制备均匀尺寸的含有难溶性药物的聚合物纳米颗粒的方法，包括将可生物降解聚合物溶解在非挥发性物质中的第一步骤 极性有机溶剂，向水中添加难溶性药物和生物降解性聚合物溶液以产生分散体的第二步骤，以及在低机械能级搅拌的条件下将分散体分批加入到乳化剂溶液中的第三步骤。 本发明的聚合物纳米颗粒能够通过使用非挥发性极性溶剂，特别是极性类似于水的溶剂的简单方法作为混合物的溶剂来制造纳米尺寸的小而均匀的聚合物纳米颗粒 可生物降解聚合物和难溶性物质的溶液，并且使用低机械能条件和在乳化过程中分批添加分散体。 本发明的聚合物纳米颗粒的优点在于，包含在聚合物颗粒中的难溶性药物的溶解速度显着提高，并且难溶性药物在长时间内逐渐稳定地释放并保持在恒定的密度。

公开(公告)号：US07652370B2

公开(公告)日：2010-01-26

申请号：US10899177

申请日：2004-07-27

申请人： Dae Sik Lee ,  Hae Sik Yang ,  Yong Taik Lim ,  Kwang Hyo Chung ,  Sung Jin Kim ,  Se Ho Park ,  Kyu Won Kim ,  Yun Tae Kim

发明人： Dae Sik Lee ,  Hae Sik Yang ,  Yong Taik Lim ,  Kwang Hyo Chung ,  Sung Jin Kim ,  Se Ho Park ,  Kyu Won Kim ,  Yun Tae Kim

IPC分类号： H01L29/06

CPC分类号： B01J19/0093 ,  B01J2219/00495 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00635 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00783 ,  B01J2219/00804 ,  B01J2219/00833 ,  B01J2219/00853 ,  B01J2219/00858 ,  B01J2219/00873 ,  B01J2219/00961 ,  B01L3/502707 ,  B01L3/50851 ,  B01L2200/12 ,  B01L2200/147 ,  B01L2300/0819 ,  B01L2300/12 ,  B01L2300/1827 ,  B33Y80/00 ,  C40B60/14 ,  G01N27/128

摘要： Provided are a plastic microfabricated structure, and a microfabricated thermal device, a microfabricated reactor, a microfabricated reactor array and a micro array using the same, which may be applied to a bio chip, and the present invention may fabricate the plastic microfabricated structure for providing a heating region by means of insulating plastic, which has a thin thickness, flatness enough to allow a photolithography process to be performed, thermal isolation in its some or total area, and a small thermal mass, and on top of the heating region of the plastic microfabricated structure, a heater, a temperature sensor for sensing a temperature, an electrode, and an electrode pad are formed to thereby fabricate the microfabricated heating device, whereby element may be readily fabricated at a low cost, and the heating region is formed of a plastic thin layer, so that uniform temperature control is possible even with a low power, and various samples may be thermally treated at a fast speed to obtain their reaction and analysis.

摘要翻译： 本发明提供一种塑料微制造结构，微制造热装置，微制造反应器，微制造反应器阵列和使用其的微阵列，其可以应用于生物芯片，并且本发明可以制造塑料微制造结构以提供 通过绝缘塑料的加热区域，其具有薄的厚度，足够的平坦度，以允许执行光刻工艺，其一些或总面积的热隔离和小的热质量，以及在 形成塑料微加工结构，加热器，用于感测温度的温度传感器，电极和电极焊盘，从而制造微制造加热装置，从而可以以低成本容易地制造元件，并且加热区域由 塑料薄层，使得即使在低功率下也能进行均匀的温度控制，并且各种样品可以在fa处进行热处理 速度获得反应和分析。

公开(公告)号：US5673084A

公开(公告)日：1997-09-30

申请号：US493392

申请日：1995-06-21

申请人： Jae Cheol Lim ,  Myung Keon Yeo ,  Yong Taik Lim ,  Nam Su Lee ,  Jim Woo Seo ,  Dae Sul Shim

发明人： Jae Cheol Lim ,  Myung Keon Yeo ,  Yong Taik Lim ,  Nam Su Lee ,  Jim Woo Seo ,  Dae Sul Shim

IPC分类号： H04N5/225 ,  H04N5/232 ,  H04N5/74

CPC分类号： G03B17/54 ,  H04N5/23293 ,  H04N5/7441 ,  H04N9/3176

摘要： A movie camera system having a function for projecting an image of an object being presently or previously imaged on an external screen, as well as a function for monitoring the image and a method of displaying a video signal therein. A composite video signal from a camera section or a VCR section is processed by a video signal processor in a display section so that it can be displayed on a display device including a matrix of pixels. The processed video signal from the video signal processor includes red, green and blue color signals. Arrangement of the proposed video signal is changed according to whether the projecting function or the view finding function is selected. According to the selected function, an optical image resulting from the video signal displayed on the display device is lighted by a large or small amount or light from a light source at the rear. The user can view or monitor the image on the display device through a focus lens unit at a front portion thereof. The image on the display device is also projected on an external screen by a large amount of light from the light source. A memory may be used to change the arrangement of the video signal before application to the display section. In this case, the display section functions only to sequentially display the video signals therein.

摘要翻译： 具有用于投影当前或先前成像在外部屏幕上的对象的图像的功能的电影摄像机系统以及用于监视图像的功能以及在其中显示视频信号的方法。 来自相机部分或VCR部分的复合视频信号由显示部分中的视频信号处理器处理，使得其可以显示在包括像素矩阵的显示装置上。 来自视频信号处理器的经处理的视频信号包括红色，绿色和蓝色信号。 所提出的视频信号的布置根据是否选择投影功能或查看功能而改变。 根据所选择的功能，由显示装置上显示的视频信号产生的光学图像被大量或少量的光照射在后面的光源处。 用户可以通过其前部的聚焦透镜单元来观看或监视显示设备上的图像。 显示装置上的图像也通过来自光源的大量光投射在外部屏幕上。 可以使用存储器来在施加到显示部分之前改变视频信号的布置。 在这种情况下，显示部分仅用于顺序地显示其中的视频信号。

公开(公告)号：US08697389B2

公开(公告)日：2014-04-15

申请号：US12937875

申请日：2009-03-24

申请人： Bong Hyun Chung ,  Yong Taik Lim

发明人： Bong Hyun Chung ,  Yong Taik Lim

IPC分类号： C12Q1/02

CPC分类号： G01N33/532 ,  B82Y15/00 ,  G01N33/5005 ,  G01N33/582 ,  G01N33/587 ,  G01N33/588

摘要： The present invention relates to cell labeling and imaging using a multifunctional perfluorocarbon (PFC) nanoemulsion. In particular, the perfluorocarbon nanoemulsion containing optical nanoparticle is provided with both optical characteristics and 19F magnetic resonance characteristics, thus can be used for magnetic resonance imaging (MRI) and optical imaging (OI) simultaneously. Cell labeling and imaging using the perfluorocarbon nanoemulsion containing quantum dot nanoparticle exerted no effect on cell viability. Therefore, perfluorocarbon nanoemulsion can be applied to methods for multifunctional and effectively cell labeling and imaging.

摘要翻译： 本发明涉及使用多功能全氟化碳（PFC）纳米乳液的细胞标记和成像。 特别是，含有光学纳米颗粒的全氟化碳纳米乳液具有光学特性和19F磁共振特性，因此可同时用于磁共振成像（MRI）和光学成像（OI）。 使用含有量子点纳米颗粒的全氟化碳纳米乳液的细胞标记和成像对细胞活力没有影响。 因此，全氟化碳纳米乳液可用于多功能，有效的细胞标记和成像方法。

公开(公告)号：US20120088261A1

公开(公告)日：2012-04-12

申请号：US12937875

申请日：2009-03-24

申请人： Bong Hyun Chung ,  Yong Taik Lim

发明人： Bong Hyun Chung ,  Yong Taik Lim

IPC分类号： C12Q1/02 ,  B82Y5/00

CPC分类号： G01N33/532 ,  B82Y15/00 ,  G01N33/5005 ,  G01N33/582 ,  G01N33/587 ,  G01N33/588

摘要： The present invention relates to cell labeling and imaging using a multifunctional perfluorocarbon (PFC) nanoemulsion. In particular, the perfluorocarbon nanoemulsion containing optical nanoparticle is provided with both optical characteristics and 19F magnetic resonance characteristics, thus can be used for magnetic resonance imaging (MRI) and optical imaging (OI) simultaneously. Cell labeling and imaging using the perfluorocarbon nanoemulsion containing quantum dot nanoparticle exerted no effect on cell viability. Therefore, perfluorocarbon nanoemulsion can be applied to methods for multifunctional and effectively cell labeling and imaging.

摘要翻译： 本发明涉及使用多功能全氟化碳（PFC）纳米乳液的细胞标记和成像。 特别是，含有光学纳米颗粒的全氟化碳纳米乳液具有光学特性和19F磁共振特性，因此可同时用于磁共振成像（MRI）和光学成像（OI）。 使用含有量子点纳米颗粒的全氟化碳纳米乳液的细胞标记和成像对细胞活力没有影响。 因此，全氟化碳纳米乳液可用于多功能，有效的细胞标记和成像方法。

公开(公告)号：US20110110867A1

公开(公告)日：2011-05-12

申请号：US13000197

申请日：2009-06-19

申请人： Bong Hyun Chung ,  Yong Taik Lim

发明人： Bong Hyun Chung ,  Yong Taik Lim

IPC分类号： A61K49/06 ,  A61K49/00 ,  B82Y15/00

CPC分类号： A61K49/0078 ,  A61K49/0002 ,  A61K49/0017 ,  A61K49/0067 ,  A61K49/1806

摘要： The present invention relates to a multimodal imaging method using a nano-emulsion comprising optical nano-particles and perfluorocarbons, and more particularly, to a method of obtaining multimodal images using a nano-emulsion consisted of perfluorocarbons for multispectral magnetic resonance imaging (MRI) and nano-particles for multicolor fluorescent detection. In the multimodal imaging method, various multispectral MRI images and multicolor fluorescent rays can be obtained by varying the kind and combination of perfluorocarbons and the kind and combination of optical nano-particles, so that multiplexed analysis is possible. Accordingly, the multimodal imaging method can be applied to various fields, including biological and medical fields in which studies on cell molecular imaging has been conducted.

摘要翻译： 本发明涉及使用包含光学纳米颗粒和全氟化碳的纳米乳液的多模式成像方法，更具体地说，涉及使用由全氟化碳组成的纳米乳液用于多光谱磁共振成像（MRI）和 用于多色荧光检测的纳米颗粒。 在多模式成像方法中，通过改变全氟化碳的种类和组合以及光学纳米颗粒的种类和组合，可以获得各种多光谱MRI图像和多色荧光光谱，从而可以进行多重分析。 因此，多模式成像方法可以应用于已经进行细胞分子成像研究的生物和医学领域的各个领域。