公开(公告)号：US20060199185A1

公开(公告)日：2006-09-07

申请号：US10564880

申请日：2003-12-31

申请人： Young Song ,  Haryoung Poo ,  Moon-Hee Sung ,  Seung-Pyo Hong ,  Yoon Ho Choi ,  Kwang Kim ,  Il Han Lee ,  Je Hyun Park

发明人： Young Song ,  Haryoung Poo ,  Moon-Hee Sung ,  Seung-Pyo Hong ,  Yoon Ho Choi ,  Kwang Kim ,  Il Han Lee ,  Je Hyun Park

IPC分类号： C40B40/02 ,  C40B40/08 ,  C12Q1/68 ,  C12N15/74

CPC分类号： C12N15/64

摘要： The present invention relates to a plasmid (pHCE-FOREX) functioning as both a T-vector and an expression vector, which is produced by imparting a T-vector function to an HCE promoter derived from a constitutive high-level expression vector and can express a target protein in a simple and rapid manner. Also, the present invention relates to an expression vector having the target gene inserted into the plasmid, and the expression of the target gene using the same. The plasmid of the present invention can be converted into a vector that expresses the target protein by one-step T-vector cloning in a simple and rapid manner. The plasmid converted into the expression vector does not require a re-transformation step and allows the high-level expression of the target protein only by the culturing of transformed E. coli without the addition of an expensive inducer. Thus, according to the present invention, expression plasmids for large amounts of target genes can be produced at the same time, so that the present invention will be very efficient in establishing expression systems for certain genomes and gene groups.

摘要翻译： 本发明涉及用作T载体和表达载体两者的质粒（pHCE-FOREX），其通过赋予来自组成型高水平表达载体的HCE启动子产生T载体功能而产生，并可表达 目标蛋白以简单快速的方式。 此外，本发明涉及将靶基因插入质粒的表达载体，以及使用该表达载体的靶基因的表达。 本发明的质粒可通过简单快速的一步T载体克隆转化为表达靶蛋白的载体。 转化成表达载体的质粒不需要再转化步骤，并且仅通过培养转化的大肠杆菌而不添加昂贵的诱导物才允许靶蛋白的高水平表达。 因此，根据本发明，可以同时生产大量靶基因的表达质粒，从而本发明在建立某些基因组和基因组的表达系统方面将非常有效。

公开(公告)号：US20090186804A1

公开(公告)日：2009-07-23

申请号：US12159561

申请日：2006-12-22

申请人： Moon-Hee Sung ,  Chung Park ,  Seung-Pyo Hong ,  Haryoung Poo ,  Tae-Woo Kim

发明人： Moon-Hee Sung ,  Chung Park ,  Seung-Pyo Hong ,  Haryoung Poo ,  Tae-Woo Kim

IPC分类号： A61K38/00 ,  A61P7/02

CPC分类号： A61K31/74 ,  A23L33/175 ,  A61K9/0095

摘要： The present invention relates to an anticoagulant and a composition for preventing thrombus formation, which contain poly-gamma-glutamic acid (PGA) as an active ingredient. The inventive PGA is a water-soluble, anionic, biodegradable and edible amino acid polymer material, which has an anticoagulant effect of preventing thrombi from being accumulated in blood vessels, shows an excellent sustained-release effect and is harmless to the human body. Thus, it is useful as a high-value-added anticoagulant and a food or beverage composition for preventing thrombus formation.

摘要翻译： 本发明涉及含有聚-γ-谷氨酸（PGA）作为活性成分的用于预防血栓形成的抗凝血剂和组合物。 本发明的PGA是一种水溶性，阴离子，可生物降解和可食用的氨基酸聚合物材料，其具有防止血栓在血管中积聚的抗凝作用，显示出优异的持续释放作用并对人体无害。 因此，作为高附加值抗凝剂和用于预防血栓形成的食品或饮料组合物是有用的。

公开(公告)号：US08618057B2

公开(公告)日：2013-12-31

申请号：US13444324

申请日：2012-04-11

申请人： Moon-Hee Sung ,  Chung Park ,  Seung-Pyo Hong ,  Haryoung Poo ,  Tae-Woo Kim

发明人： Moon-Hee Sung ,  Chung Park ,  Seung-Pyo Hong ,  Haryoung Poo ,  Tae-Woo Kim

IPC分类号： A61K38/02

CPC分类号： A61K31/74 ,  A23L33/175 ,  A61K9/0095

摘要： The present invention relates to an anticoagulant and a composition for preventing thrombus formation, which contain poly-gamma-glutamic acid (PGA) as an active ingredient. The inventive PGA is a water-soluble, anionic, biodegradable and edible amino acid polymer material, which has an anticoagulant effect of preventing thrombi from being accumulated in blood vessels, shows an excellent sustained-release effect and is harmless to the human body. Thus, it is useful as a high-value-added anticoagulant and a food or beverage composition for preventing thrombus formation.

摘要翻译： 本发明涉及含有聚-γ-谷氨酸（PGA）作为活性成分的用于预防血栓形成的抗凝血剂和组合物。 本发明的PGA是一种水溶性，阴离子，可生物降解和可食用的氨基酸聚合物材料，其具有防止血栓在血管中积聚的抗凝作用，显示出优异的持续释放作用并对人体无害。 因此，作为高附加值抗凝剂和用于预防血栓形成的食品或饮料组合物是有用的。

公开(公告)号：US20110318384A1

公开(公告)日：2011-12-29

申请号：US13143700

申请日：2010-01-08

申请人： Moon-Hee Sung ,  Haryoung Poo ,  Il Han Lee

发明人： Moon-Hee Sung ,  Haryoung Poo ,  Il Han Lee

IPC分类号： A61K39/12 ,  A61P37/04 ,  A61P35/00 ,  C12N15/74 ,  C12N1/21

CPC分类号： C12N15/746 ,  A61K39/12 ,  A61K2039/523 ,  A61K2039/542 ,  C12N2710/20034 ,  C12N2710/20045 ,  C12N2810/55

摘要： A surface expression vector for preparing HPV vaccines, in which the surface expression vector contains a gene encoding a repE mutant protein having an amino acid sequence of SEQ ID NO: 1, a promoter, a poly-gamma-glutamate synthetase complex gene, and a gene which is linked with the poly-gamma-glutamate synthetase complex gene and encodes a tumor induction-associated antigen protein of human papillomavirus.An expression vector constitutively expressing a high level of the human papillomavirus (HPV) antigen protein is provided. Also, a recombinant lactic acid bacteria, transformed with the expression vector and expressing the HPV antigen protein on the surface thereof, and a composition comprising the recombinant lactic acid bacteria are provided. The recombinant lactic acid bacteria and the composition are very effective as a vaccine for the treatment of cervical cancer, because they can be applied orally or directly to the vagina.

摘要翻译： 用于制备HPV疫苗的表面表达载体，其中所述表面表达载体含有编码具有SEQ ID NO：1的氨基酸序列的repE突变蛋白的基因，启动子，聚-γ-谷氨酸合成酶复合物基因和 基因，其与多聚-γ-谷氨酸合成酶复合物基因连接并编码人乳头瘤病毒的肿瘤诱导相关抗原蛋白。 提供了组成型表达高水平人乳头瘤病毒（HPV）抗原蛋白的表达载体。 此外，提供了用表达载体转化并在其表面上表达HPV抗原蛋白的重组乳酸菌和包含重组乳酸菌的组合物。 重组乳酸菌和组合物作为用于治疗子宫颈癌的疫苗非常有效，因为它们可以口服或直接施用于阴道。

公开(公告)号：US20100256050A1

公开(公告)日：2010-10-07

申请号：US12678027

申请日：2007-09-13

申请人： Moon-Hee Sung ,  Chul Joong Kim ,  Haryoung Poo ,  Young-Ki Choi ,  Il Han Lee ,  Dai-Won Yoo

发明人： Moon-Hee Sung ,  Chul Joong Kim ,  Haryoung Poo ,  Young-Ki Choi ,  Il Han Lee ,  Dai-Won Yoo

IPC分类号： A61K38/16 ,  A61P31/12

CPC分类号： A61K31/785 ,  A61K38/164

摘要： The present invention relates to a pharmaceutical composition for inhibiting or preventing viral infection, which comprises an effective dose of poly-gamma-glutamic acid (γ-PGA), and more particularly, to a pharmaceutical composition, a functional food, and a feedstuff additive capable of inhibiting viral infection and preventing viral diseases, which comprise poly-gamma-glutamic acid having an infection-inhibiting effect against viruses, such as an influenza virus, inducing respiratory infection or systemic infection, as an effective ingredient.The composition containing poly-gamma-glutamic acid as an effective ingredient according to the present invention, is effective for use as an animal feedstuff additive or a pharmaceutical agent for preventing influenza virus infection and various viral diseases as well as a pharmaceutical composition and a functional food to promote human health.

摘要翻译： 本发明涉及用于抑制或预防病毒感染的药物组合物，其包含有效剂量的聚-γ-谷氨酸（γ-PGA），更具体地，涉及药物组合物，功能性食品和饲料添加剂 能够抑制病毒感染和预防病毒性疾病，其包含对病毒（例如流感病毒）诱导呼吸道感染或全身感染具有感染抑制作用的聚-γ-谷氨酸作为有效成分。 含有根据本发明的有效成分的聚-γ-谷氨酸的组合物可用作动物饲料添加剂或用于预防流感病毒感染和各种病毒性疾病的药剂以及药物组合物和功能性 食物促进人体健康。

公开(公告)号：US09095605B2

公开(公告)日：2015-08-04

申请号：US13234670

申请日：2011-09-16

申请人： Moon-Hee Sung ,  Chul Joong Kim ,  Haryoung Poo ,  Young-Ki Choi ,  Il Han Lee ,  Dai-Won Yoo

发明人： Moon-Hee Sung ,  Chul Joong Kim ,  Haryoung Poo ,  Young-Ki Choi ,  Il Han Lee ,  Dai-Won Yoo

IPC分类号： A61K38/16 ,  A61K31/785

CPC分类号： A61K31/785 ,  A61K38/164

摘要： The present invention relates to a pharmaceutical composition for inhibiting or preventing viral infection, which comprises an effective dose of poly-gamma-glutamic acid (γ-PGA), and more particularly, to a pharmaceutical composition, a functional food, and a feedstuff additive capable of inhibiting viral infection and preventing viral diseases, which comprise poly-gamma-glutamic acid having an infection-inhibiting effect against viruses, such as an influenza virus, inducing respiratory infection or systemic infection, as an effective ingredient.The composition containing poly-gamma-glutamic acid as an effective ingredient according to the present invention, is effective for use as an animal feedstuff additive or a pharmaceutical agent for preventing influenza virus infection and various viral diseases as well as a pharmaceutical composition and a functional food to promote human health.

摘要翻译： 本发明涉及用于抑制或预防病毒感染的药物组合物，其包含有效剂量的聚-γ-谷氨酸（γ-PGA），更具体地，涉及药物组合物，功能性食品和饲料添加剂 能够抑制病毒感染和预防病毒性疾病，其包含对病毒（例如流感病毒）诱导呼吸道感染或全身感染具有感染抑制作用的聚-γ-谷氨酸作为有效成分。 含有根据本发明的有效成分的聚-γ-谷氨酸的组合物可用作动物饲料添加剂或用于预防流感病毒感染和各种病毒性疾病的药剂以及药物组合物和功能性 食物促进人体健康。

公开(公告)号：US08685721B2

公开(公告)日：2014-04-01

申请号：US13143700

申请日：2010-01-08

申请人： Moon-Hee Sung ,  Haryoung Poo ,  Il Han Lee

发明人： Moon-Hee Sung ,  Haryoung Poo ,  Il Han Lee

IPC分类号： C12N15/37 ,  A61K39/12 ,  A61K39/07 ,  A61K39/09

CPC分类号： C12N15/746 ,  A61K39/12 ,  A61K2039/523 ,  A61K2039/542 ,  C12N2710/20034 ,  C12N2710/20045 ,  C12N2810/55

摘要： A surface expression vector for preparing HPV immunogenic compositions, in which the surface expression vector contains a gene encoding a repE mutant protein having an amino acid sequence of SEQ ID NO: 1, a promoter, a poly-gamma-glutamate synthetase complex gene, and a gene which is linked with the poly-gamma-glutamate synthetase complex gene and encodes a tumor induction-associated antigen protein of human papillomavirus.An expression vector constitutively expressing a high level of the human papillomavirus (HPV) antigen protein is provided. Also, a recombinant lactic acid bacteria, transformed with the expression vector and expressing the HPV antigen protein on the surface thereof, and a composition comprising the recombinant lactic acid bacteria are provided. The recombinant lactic acid bacteria and the composition are very effective as a immunogenic composition for the treatment of cervical cancer, because they can be applied orally or directly to the vagina.

摘要翻译： 用于制备HPV免疫原性组合物的表面表达载体，其中所述表面表达载体含有编码具有SEQ ID NO：1的氨基酸序列的repE突变蛋白的基因，启动子，聚-γ-谷氨酸合成酶复合物基因和 与聚-γ-谷氨酸合成酶复合物基因连接并编码人乳头瘤病毒的肿瘤诱导相关抗原蛋白的基因。 提供了组成型表达高水平人乳头瘤病毒（HPV）抗原蛋白的表达载体。 此外，提供了用表达载体转化并在其表面上表达HPV抗原蛋白的重组乳酸菌和包含重组乳酸菌的组合物。 重组乳酸菌和组合物作为用于治疗子宫颈癌的免疫原性组合物是非常有效的，因为它们可以口服或直接施用于阴道。

公开(公告)号：US20100255040A1

公开(公告)日：2010-10-07

申请号：US12682662

申请日：2007-10-15

申请人： Moon-Hee Sung ,  Chul Joong Kim ,  Haryoung Poo ,  Il Han Lee ,  Yang-Hyun Kim

发明人： Moon-Hee Sung ,  Chul Joong Kim ,  Haryoung Poo ,  Il Han Lee ,  Yang-Hyun Kim

IPC分类号： A61K39/00 ,  A61P31/12 ,  A61P31/04 ,  A61P35/00

CPC分类号： A61K31/765

摘要： The present invention relates to a composition for improving cellular immunity, which comprises an effective dose of poly-gamma-glutamic acid, and more particularly, to a composition for improving cellular immunity comprising poly-gamma-glutamic acid, which has effects of inducing enhancement of TLR-mediated Th1 cellular immunity and improving antigen presenting activity to maintain the improved activity. The inventive composition comprising an effective dose of poly-gamma-glutamic acid has very little toxicity and side effects, and can be added to a vaccine composition for preventing and treating animal viral or bacterial infections and cancer, or a vaccine composition for preventing and treating human viral or bacterial infections and cancer to show cellular immunity-enhancing effects.

摘要翻译： 本发明涉及一种用于改善细胞免疫的组合物，其包含有效剂量的聚-γ-谷氨酸，更具体地，涉及包含多聚γ-谷氨酸的组合物，所述组合物具有诱导增强的作用 的TLR介导的Th1细胞免疫并改善抗原呈递活性以维持改善的活性。 包含有效剂量的聚-γ-谷氨酸的本发明组合物具有非常少的毒性和副作用，并且可以加入用于预防和治疗动物病毒或细菌感染和癌症的疫苗组合物或用于预防和治疗的疫苗组合物 人类病毒或细菌感染和癌症显示细胞免疫增强作用。

公开(公告)号：US20060140971A1

公开(公告)日：2006-06-29

申请号：US10559631

申请日：2004-06-04

申请人： Moon-Hee Sung ,  Chul-Joong Kim ,  Chang-Min Jung ,  Seung-Pyo Hong ,  Jong Su Lee ,  Jae Choi ,  Kwang Kim ,  Kuroda Shunichi ,  Ha Ryoung Poo

发明人： Moon-Hee Sung ,  Chul-Joong Kim ,  Chang-Min Jung ,  Seung-Pyo Hong ,  Jong Su Lee ,  Jae Choi ,  Kwang Kim ,  Kuroda Shunichi ,  Ha Ryoung Poo

IPC分类号： A61K39/02 ,  C12N1/21 ,  C12N15/74 ,  C07K14/165

CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K2039/523 ,  C07K2319/00 ,  C12N9/93 ,  C12N15/74 ,  C12N2710/20022 ,  C12N2770/20022 ,  Y02A50/476

摘要： The present invention relates to a surface expression vector of SARS coronavirus antigen containing a gene encoding an antigen of SARS inducing coronavirus and any one or two or more of genes pgsB, pgsC and pgsA encoding poly-gamma-glutamic acid synthase complex, a microorganism transformed by the surface expression vector, and a SARS vaccine comprising the microorganism. According to the present invention, it is possible to economically produce a vaccine for prevention and treatment of SARS using a recombinant strain expressing an SARS coronavirus antigen on their surface.

摘要翻译： 本发明涉及SARS冠状病毒抗原的表达表达载体，其含有编码SARS诱导冠状病毒抗原的基因和编码聚-γ-谷氨酸合酶复合物的pgsB，pgsC和pgsA基因中的一种或两种以上的基因，微生物转化 通过表面表达载体和包含该微生物的SARS疫苗。 根据本发明，可以在表面上使用表达SARS冠状病毒抗原的重组菌株经济地制备用于预防和治疗SARS的疫苗。

公开(公告)号：US06967087B1

公开(公告)日：2005-11-22

申请号：US10220174

申请日：2000-07-14

申请人： Moon-Hee Sung ,  Seung-Goo Lee ,  Sung-Keun Rhee ,  Seung-Pyo Hong ,  Dae-Joong Kang ,  Su-Mi Kim ,  Chul-Joong Kim ,  Jin-Woo Bae ,  Che Ok Jeon ,  Joong-Jae Kim ,  Kwang Kim ,  Jae Jun Song

发明人： Moon-Hee Sung ,  Seung-Goo Lee ,  Sung-Keun Rhee ,  Seung-Pyo Hong ,  Dae-Joong Kang ,  Su-Mi Kim ,  Chul-Joong Kim ,  Jin-Woo Bae ,  Che Ok Jeon ,  Joong-Jae Kim ,  Kwang Kim ,  Jae Jun Song

IPC分类号： G01N33/569 ,  C12N1/20 ,  C12N9/88 ,  C12P13/22 ,  C12Q1/06 ,  C12R1/01 ,  G01N21/77 ,  C12P39/00 ,  C12Q1/12

CPC分类号： C12R1/01 ,  C12N9/88 ,  C12P13/227

摘要： The present invention relates to a novel obligately symbiotic thermophile Symbiobacterium toebii SC-1(Accession NO: KCTC 0685BP), and a thermostable L-tyrosine phenol-lyase and L-tryptophan indole-lyase produced by Symbiobacterium toebii SC-1. The present invention also relates to a pure culture method of Symbiobacterium toebii SC-1, a method of growth measurement for Symbiobacterium toebu SC-1 and its relative symbiotic bacteria showing low-growth yield using nitrate respiration, and a screening method of relative symbiotic strains of Symbiobacterium toebii SC-1 from the environment using a specific antibody to it. Therefore, the thermostable L-tyrosine phenol-lyase and L-tryptophan indole-lyase produced by Symbiobacterium toebii SC-1 in this invention can provide more stable catalysts in enzymatic biotransformation processes which enzymatically produce valuable medico-amino acids 3,4-dehydroxy-L-phenylalanine (L-DOPA) and L-tryptophan.

摘要翻译： 本发明涉及一种新型的共生共生嗜热ile ile i i i i i i i i））i i i i i i i i i i i i i i i i i。。。。。。。。。。。。。。。。。。。。 本发明还涉及使用硝酸盐呼吸的共生杆菌SC-1的纯培养方法，使用硝酸盐呼吸显示低生长量的共生杆菌SC-1及其相对共生菌的生长测定方法以及相对共生菌株的筛选方法 的来自环境的共表达杆菌SC-1使用特异性抗体。 因此，在本发明中由共生杆菌（Toibii）SC-1产生的热稳定性L-酪氨酸苯酚裂解酶和L-色氨酸吲哚裂解酶可以在酶生物转化过程中提供更稳定的催化剂，其中酶促产生有价值的羟基 - 氨基酸3,4-脱羟基 - L-苯丙氨酸（L-DOPA）和L-色氨酸。