公开(公告)号：US4886749A

公开(公告)日：1989-12-12

申请号：US54975

申请日：1987-05-28

申请人： Munehiko Dombou ,  Isao Tomioka ,  Senji Kitabatake ,  Hiroshi Nakejima

发明人： Munehiko Dombou ,  Isao Tomioka ,  Senji Kitabatake ,  Hiroshi Nakejima

IPC分类号： C12N9/00 ,  C12P19/36

CPC分类号： C12N9/93 ,  C12P19/36 ,  Y10S435/822 ,  Y10S435/832

摘要： A process for producing diadenosine tetraphosphate or derivatives thereof in very high yields with little formation of undesired by-products is disclosed. The process comprises reacting adenosine-5'-triphosphate or a derivative thereof with an amino acid under the catalytic action of an aminoacyl-tRNA synthetase in the presence of an enzyme that converts adenosine-5'-diphosphate to adenosine-5'-triphosphate. The formation of by-products can be substantially completely inhibited if the last-mentioned enzyme is used in combination with an enzyme that converts adenosine-5'-monophosphate to adenosine-5'-diphosphate.

摘要翻译： 公开了以非常高的产率生产二腺苷四磷酸或其衍生物的方法，几乎​​没有形成不需要的副产物。 该方法包括在氨基-5'-二磷酸腺苷转化为腺苷-5'-三磷酸的酶存在下，在氨基-tRNA合成酶的催化作用下使腺苷-5'-三磷酸或其衍生物与氨基酸反应。 如果最后提到的酶与将腺苷-5'-单磷酸腺苷转化为腺苷-5'-二磷酸的酶组合使用，副产物的形成可以基本上完​​全被抑制。

公开(公告)号：US5294546A

公开(公告)日：1994-03-15

申请号：US799881

申请日：1991-11-29

申请人： Munehiko Dombou ,  Isao Tomioka ,  Ryoichi Tsurutani ,  Senji Kitabatake ,  Hiroshi Nakajima

发明人： Munehiko Dombou ,  Isao Tomioka ,  Ryoichi Tsurutani ,  Senji Kitabatake ,  Hiroshi Nakajima

IPC分类号： C12N1/38 ,  C12N1/20 ,  C12P19/00 ,  C12P19/02 ,  C12P19/04 ,  C12R1/645 ,  C12R1/88

CPC分类号： C12N1/20 ,  C12N1/38 ,  C12P19/02 ,  C12P19/04 ,  Y10S435/921 ,  Y10S435/922 ,  Y10S435/923 ,  Y10S435/924 ,  Y10S435/938 ,  Y10S435/944

摘要： A method of producing a growth promoting factor for Bifidobacterium species from lactose which comprises contacting lactose with resting cells of a lactose-utilizing yeast strain having activity to rearrange lactose to galacto-oligosaccharides.

摘要翻译： 一种从乳糖生产双歧杆菌物种生长促进因子的方法，其包括将乳糖与具有将乳糖重排乳糖寡糖的活性的乳糖利用酵母菌株的静息细胞接触。

公开(公告)号：US5001055A

公开(公告)日：1991-03-19

申请号：US862731

申请日：1986-05-13

申请人： Kazutomo Imahori ,  Isao Tomioka ,  Hiroshi Nakajima ,  Senji Kitabatake

发明人： Kazutomo Imahori ,  Isao Tomioka ,  Hiroshi Nakajima ,  Senji Kitabatake

IPC分类号： C07H19/20 ,  C12P1/00 ,  C12P13/00 ,  C12P13/04 ,  C12P13/14 ,  C12P19/02 ,  C12P19/32

CPC分类号： C12P13/001 ,  C07H19/20 ,  C12P1/00 ,  C12P13/00 ,  C12P13/04 ,  C12P13/06 ,  C12P13/14 ,  C12P13/20 ,  C12P19/02 ,  C12P19/32 ,  Y10S435/813

摘要： A process for producing a physiologically active substance by a combined enzymatic method is disclosed. In the combined enzymatic method, a reactant solution containing a precursor or precursors for the physiologically active substance, AXP, and a divalent metal ion is supplied at one end of a reactor incorporating either the combined enzymatic reaction system (a) or (b), wherein (a) is a reaction system including an enzyme for converting AMP to ADP, an enzyme for converting ADP to ATP, and an enzyme which catalyzes the synthesis of the physiologically active substance as it converts ATP to AMP; and (b) is a reaction system including an enzyme for converting ADP to ATP and an enzyme which catalyzes the synthesis of the physiologically active substance as it converts ATP to ADP, wherein the concentration of the divalent metal ion supplied into the reactor is held at a level no higher than 30 mM while the concentration of the AXP is held below that of that of the precursor or precursors for the physilogically active substance, and the physiologically active substance produced is withdrawn from the other end of the reactor.

摘要翻译： 公开了通过组合的酶法生产生理活性物质的方法。 在组合的酶法中，在包含组合的酶反应体系（a）或（b）的反应器的一端提供含有生理活性物质前体或前体的反应物溶液AXP和二价金属离子， 其中（a）是包含用于将AMP转化成ADP的酶的反应体系，用于将ADP转化为ATP的酶，以及当ATP转化成AMP时催化生理活性物质合成的酶; 和（b）是包含用于将ADP转化为ATP的酶的反应体系，以及当ATP向ADP转化时催化生理活性物质的合成的酶，其中供给到反应器中的二价金属离子的浓度保持在 水平不高于30mM，而AXP的浓度低于物理活性物质的前体或前体的浓度，生成的生理活性物质从反应器的另一端排出。