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公开(公告)号：US20220114819A1
公开(公告)日：2022-04-14
申请号：US17509882
申请日：2021-10-25
申请人： NIKON CORPORATION
发明人： Toru ICHIHASHI , Hiroaki KII , Tetsuomi TAKASAKI , Takayuki UOZUMI , Yoichi YAMAZAKI , Masaki KINEHARA
摘要： A cell tracking method for performing cell tracking on the basis of a plurality of cell images captured in time series, including an extraction process of extracting a tracking region corresponding to the cell for each cell images; a tracking process of calculating a change information in a position of the extracted tracking region and tracking the cell on the basis of the change information; a determination process of determining whether or not the cell as a tracking target is in a cell division state on the basis of the cell image; and an analysis process of analyzing a movement state of the cell on the basis of the change information from which the change information in a period of the cell division state is excluded when the cell is determined to be in the cell division state in the determination process.
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公开(公告)号：US20160140710A1
公开(公告)日：2016-05-19
申请号：US15008225
申请日：2016-01-27
申请人： NIKON CORPORATION
发明人： Hiroaki KII , Chisako IWAMOTO
CPC分类号： G06T7/0012 , G01N21/6458 , G06K9/00127 , G06K9/52 , G06K2009/4666 , G06T7/62 , G06T2207/10056 , G06T2207/20036 , G06T2207/30024
摘要： An image processing apparatus, a method, and a program for allowing cells to be quantitatively observed. A computer obtains a cell membrane image obtained by performing fluorescent observation on a cell membrane of a cell serving as a sample and a tricellular tight junction (tTJ) image obtained by performing fluorescent observation on a protein localized in a tTJ of the cell. The computer derives the size of area of a region of the cell by identifying the region of each cell from the cell membrane image, derives the size of area of the region of the protein localized in the cell from the tTJ image, and dividing the obtained size of area of the region of the protein by the size of area of the region of the cell, thus calculating an index of adhesion strength of the cells. The invention can be applied to an observation system.
摘要翻译： 用于允许定量观察细胞的图像处理装置，方法和程序。 计算机获得通过对作为样品的细胞的细胞膜进行荧光观察获得的细胞膜图像和通过对位于细胞的tTJ中的蛋白进行荧光观察获得的三角形紧密连接（tTJ）图像。 计算机通过从细胞膜图像识别每个细胞的区域来导出细胞区域的面积的大小，从tTJ图像中导出定位在细胞中的蛋白质区域的面积的大小，并将得到的 蛋白质区域的面积大小与细胞区域面积的大小，从而计算出细胞的粘附强度指数。 本发明可以应用于观察系统。
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公开(公告)号：US20140099014A1
公开(公告)日：2014-04-10
申请号：US14103040
申请日：2013-12-11
申请人： NIKON CORPORATION
发明人： Hiroaki KII , Chisako IWAMOTO
IPC分类号： G06K9/00
CPC分类号： G06T7/0012 , G01N21/6458 , G06K9/00127 , G06K9/52 , G06K2009/4666 , G06T7/62 , G06T2207/10056 , G06T2207/20036 , G06T2207/30024
摘要： An image processing apparatus, a method, and a program for allowing cells to be quantitatively observed. A computer obtains a cell membrane image obtained by performing fluorescent observation on a cell membrane of a cell serving as a sample and a tricellular tight junction (tTJ) image obtained by performing fluorescent observation on a protein localized in a tTJ of the cell. The computer derives the size of area of a region of the cell by identifying the region of each cell from the cell membrane image, derives the size of area of the region of the protein localized in the cell from the tTJ image, and dividing the obtained size of area of the region of the protein by the size of area of the region of the cell, thus calculating an index of adhesion strength of the cells. The invention can be applied to an observation system.
摘要翻译： 用于允许定量观察细胞的图像处理装置，方法和程序。 计算机获得通过对作为样品的细胞的细胞膜进行荧光观察获得的细胞膜图像和通过对位于细胞的tTJ中的蛋白进行荧光观察获得的三角形紧密连接（tTJ）图像。 计算机通过从细胞膜图像识别每个细胞的区域来导出细胞区域的面积的大小，从tTJ图像中导出定位在细胞中的蛋白质区域的面积的大小，并将得到的 蛋白质区域的面积大小与细胞区域面积的大小，从而计算出细胞的粘附强度指数。 本发明可以应用于观察系统。
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公开(公告)号：US20210133425A1
公开(公告)日：2021-05-06
申请号：US17148431
申请日：2021-01-13
申请人： NIKON CORPORATION
摘要： An image processing device includes: an associating unit configured to associate information on an identification element used to identify a cell in imaged cell information with information on a non-identification element serving as an element of the cell which is not the identification element on the basis of the information on the identification element and information of the non-identification element; and a status determination unit configured to determine a cell status on the basis of the information on the identification element and the information on the non-identification element associated together by the linking unit.
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公开(公告)号：US20190073517A1
公开(公告)日：2019-03-07
申请号：US16124804
申请日：2018-09-07
申请人： NIKON CORPORATION
发明人： Hiroaki KII , Yasujiro KIYOTA , Takayuki UOZUMI , Yoichi YAMAZAKI
摘要： An image processing device includes an image processing unit that performs image processing on an observed image in which a cell is imaged and an image processing method selector that is configured to determine an observed image processing method for analyzing the imaged cell on the basis of information of a processed image obtained through image processing of the image processing unit.
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公开(公告)号：US20190073516A1
公开(公告)日：2019-03-07
申请号：US16124782
申请日：2018-09-07
申请人： NIKON CORPORATION
摘要： An image processing device includes: an associating unit configured to associate information on an identification element used to identify a cell in imaged cell information with information on a non-identification element serving as an element of the cell which is not the identification element on the basis of the information on the identification element and information of the non-identification element; and a status determination unit configured to determine a cell status on the basis of the information on the identification element and the information on the non-identification element associated together by the linking unit.
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公开(公告)号：US20210292703A1
公开(公告)日：2021-09-23
申请号：US17224591
申请日：2021-04-07
发明人： Tomoro DAN , Hiroaki KII , Takayuki UOZUMI , Yoshiko SATO , Shinsuke YOSHIDA
摘要： A passage timing calculation device includes a processor and a memory encoded with instructions executed by the processor, wherein the instructions causing the processor to perform operations comprising calculating a time change in an area occupied by an extraction target region that is a region in which a striated pattern appears from a plurality of images of pluripotent stem cells captured at different times, detecting a change point of the time change in an area occupied by the extraction target region, and calculating a passage timing of the pluripotent stem cells on the basis of the change point.
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公开(公告)号：US20210125335A1
公开(公告)日：2021-04-29
申请号：US17130294
申请日：2020-12-22
申请人： NIKON CORPORATION
发明人： Hiroaki KII , Shinichi TAKAYAMA
IPC分类号： G06T7/00
摘要： A device includes an image processing unit configured to calculate color information of at least one cell in a captured image and a determination unit configured to determine a cultured state of the cell on the basis of the color information calculated by the image processing unit.
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9.发明申请公开(公告)号：US20200240975A1
公开(公告)日：2020-07-30
申请号：US16849529
申请日：2020-04-15
申请人： NIKON CORPORATION
发明人： Yasujiro KIYOTA , Hiroaki KII
IPC分类号： G01N33/483 , C12Q1/02 , C12M1/34 , C12M1/36 , G01N15/06 , G06T7/00 , G06K9/00 , G02B21/36 , G01N15/02
摘要： An observation apparatus includes: an area calculation unit that calculates a colony area based on an image in which a cell colony is captured; a cell number calculation unit that calculates, based on the image, the number of cells included in a target colony of which an area is calculated by the area calculation unit; and a density calculation unit that calculates, based on the area of the target colony calculated by the area calculation unit and the number of the cells included in the target colony calculated by the cell number calculation unit, a density of the cells included in the target colony.
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公开(公告)号：US20180180054A1
公开(公告)日：2018-06-28
申请号：US15899766
申请日：2018-02-20
申请人： NIKON CORPORATION
发明人： Hiroaki KII
IPC分类号： F04D29/02 , F04D29/058 , F04D29/059 , F04D29/053 , F16C3/02 , F16C35/04
CPC分类号： F04D29/023 , F04D29/053 , F04D29/058 , F04D29/059 , F05D2230/41 , F05D2230/90 , F05D2300/2263 , F05D2300/506 , F05D2300/701 , F16C3/02 , F16C35/04 , F16C2360/44 , G01N21/6408 , G01N21/6458 , G02B21/367
摘要： A certain material irregularly expressed in an observation area is effectively observed. An observing apparatus includes a first observing unit performing a time lapse shooting of a predetermined observation area, a first discriminating unit discriminating whether or not a first material is expressed in the observation area based on an image obtained by the first observing unit, and a second observing unit starting a time lapse shooting relating to a part where the first material is expressed at a timing when the first material is expressed in the observation area, in which a shooting frequency of the time lapse shooting by the second observing unit is higher than a shooting frequency of the time lapse shooting by the first observing unit.
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