HIGH-THROUGHPUT 3D PRINTING OF CUSTOMIZED ASPHERIC IMAGING LENSES

    公开(公告)号:US20210016496A1

    公开(公告)日:2021-01-21

    申请号:US16975458

    申请日:2019-03-08

    Abstract: In an aspect, a method for additive manufacture of a three-dimensional object based on a computational model comprises steps of: grayscale photohardening a precursor material to form a portion of the object; and applying a hardened meniscus coating at a feature of the object; wherein the three-dimensional object is formed via at least the combination of the steps of gray scale photohardening and applying the meniscus coating. In some embodiments, the grayscale photohardening step is a grayscale photopolymerization step. In some embodiments, the applying a hardened meniscus coating step is a meniscus equilibrium post-curing step.

    SPECTROSCOPIC SINGLE-MOLECULE LOCALIZATION MICROSCOPY

    公开(公告)号:US20210396982A1

    公开(公告)日:2021-12-23

    申请号:US17323302

    申请日:2021-05-18

    Abstract: A method of microscopy comprises collecting an emission light; symmetrically dispersing the collected emission light into a first order (“1st”) light and a negative first order (“−1st”) light using a grating; wherein the 1st light comprises spectral information and the −1st light comprises spectral information; capturing the 1st light and the −1st light using a camera, localizing the one or more light-emitting materials using localization information determined from both the first spectral image and the second spectral image; and determining spectral information from the one or more light-emitting materials using the first spectral image and/or the second spectral image; wherein the steps of localizing and obtaining are performed simultaneously. A spectrometer for a microscope comprises a dual-wedge prism (“DWP”) for receiving and spectrally dispersing a light beam, wherein the DWP comprises a first dispersive optical device and a second dispersive optical device adhered to each other.

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