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公开(公告)号：US20150218567A1
公开(公告)日：2015-08-06
申请号：US14426557
申请日：2013-09-26
Applicant: Randy BERKA , Barbara CHERRY , NOVOZYMES, INC.
Inventor： Randy Berka , Barbara Cherry
CPC classification number: C12N15/75 , C07K14/32 , C12P5/007 , C12P5/02 , C12P5/026 , C12P7/04 , C12P13/04 , C12P21/00 , C12P21/02 , Y02E50/343
Abstract: Provided herein are Bacillus mutants having improved transformation efficiency, comprising a disruption to an endogenous mecA gene and disruption to an endogenous sinI gene. Also described are methods for producing the mutants, methods for generating transformants using the mutants, and methods for producing a polypeptide or fermentation product using the mutants.
Abstract translation: 本文提供了具有改善的转化效率的芽孢杆菌突变体，包括内源性mecA基因的破坏和内源性sinI基因的破坏。 还描述了用于产生突变体的方法，使用突变体产生转化体的方法，以及使用突变体产生多肽或发酵产物的方法。
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公开(公告)号：US08685738B2
公开(公告)日：2014-04-01
申请号：US13760763
申请日：2013-02-06
Applicant: Novozymes, Inc.
Inventor： Randy Berka , Michelle Maranta , Maria Tang , Barbara Cherry
Abstract: The present invention relates to methods of obtaining genetic competence in non-competent Bacillus cells for their transformation with exogenous DNA.
Abstract translation: 本发明涉及在非感受态芽孢杆菌细胞中获得外源DNA转化的遗传能力的方法。
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公开(公告)号：US10316344B2
公开(公告)日：2019-06-11
申请号：US15183474
申请日：2016-06-15
Applicant: Novozymes, Inc.
Inventor： Randy Berka , Joel Cherry
IPC: C12P19/14 , C12P7/26 , C12P7/00 , C12P7/02 , C12P7/04 , C12P19/02 , C12P17/04 , C12P13/04 , C12P7/40 , C12P7/28 , C12P5/02 , C12P7/20 , C12P7/18 , C12P7/16 , C12P7/10 , C12P7/06
Abstract: The present invention relates to methods for converting plant cell wall polysaccharides into one or more products, comprising: treating the plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant microorganism, wherein the recombinant microorganism expresses one or more heterologous genes encoding enzymes which degrade or convert the plant cell wall polysaccharides into the one or more products. The present invention also relates to methods for producing an organic substance, comprising: (a) saccharifying plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant microorganism, wherein the recombinant microorganism expresses one or more heterologous genes encoding enzymes which degrade or convert the plant cell wall polysaccharides into saccharified material; (b) fermenting the saccharified material of step (a) with one or more fermenting microoganisms; and (c) recovering the organic substance from the fermentation.
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公开(公告)号：US20150259389A9
公开(公告)日：2015-09-17
申请号：US13961146
申请日：2013-08-07
Applicant: Novozymes, Inc.
Inventor： Randy Berka , Michael Rey , Preethi Ramaiya
IPC: C07K14/32
Abstract: The present invention relates to an isolated polynucleotide of the complete chromosome of Bacillus licheniformis SJ1904 (ATCC PTA-7992). The present invention also relates to isolated polynucleotides of the chromosome of Bacillus licheniformis SJ1904 that encode biologically active substances and to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing biologically active substances encoded by the polynucleotides and to methods of using the isolated polynucleotides of the complete chromosome of Bacillus licheniformis SJ1904.
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Patent Agency Ranking
公开(公告)号：US20150045535A1
公开(公告)日：2015-02-12
申请号：US13961146
申请日：2013-08-07
Applicant: Novozymes, Inc.
Inventor： Randy Berka , Michael Rey , Preethi Ramaiya
IPC: C07K14/32
Abstract: The present invention relates to an isolated polynucleotide of the complete chromosome of Bacillus licheniformis SJ1904 (ATCC PTA-7992). The present invention also relates to isolated polynucleotides of the chromosome of Bacillus licheniformis SJ1904 that encode biologically active substances and to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing biologically active substances encoded by the polynucleotides and to methods of using the isolated polynucleotides of the complete chromosome of Bacillus licheniformis SJ1904.
Abstract translation: 本发明涉及地衣芽孢杆菌SJ1904（ATCC PTA-7992）的完整染色体的分离的多核苷酸。 本发明还涉及编码生物活性物质的地衣芽孢杆菌SJ1904的染色体和包含多核苷酸的核酸构建体，载体和宿主细胞的分离的多核苷酸以及由多核苷酸编码的生物活性物质的制备方法和方法 使用地衣芽孢杆菌SJ1904的完整染色体的分离的多核苷酸。
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公开(公告)号：US20140141472A1
公开(公告)日：2014-05-22
申请号：US14157383
申请日：2014-01-16
Applicant: NOVOZYMES, INC.
Inventor： Randy Berka , Joel Cherry
CPC classification number: C12P19/14 , C12P5/023 , C12P7/00 , C12P7/02 , C12P7/04 , C12P7/06 , C12P7/10 , C12P7/16 , C12P7/18 , C12P7/20 , C12P7/26 , C12P7/28 , C12P7/40 , C12P13/04 , C12P17/04 , C12P19/02 , Y02E50/10 , Y02E50/16 , Y02E50/17 , Y02E50/343
Abstract: The present invention relates to methods for converting plant cell wall polysaccharides into one or more products, comprising: treating the plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant microorganism, wherein the recombinant microorganism expresses one or more heterologous genes encoding enzymes which degrade or convert the plant cell wall polysaccharides into the one or more products. The present invention also relates to methods for producing an organic substance, comprising: (a) saccharifying plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant microorganism, wherein the recombinant microorganism expresses one or more heterologous genes encoding enzymes which degrade or convert the plant cell wall polysaccharides into saccharified material; (b) fermenting the saccharified material of step (a) with one or more fermenting microoganisms; and (c) recovering the organic substance from the fermentation.
Abstract translation: 本发明涉及将植物细胞壁多糖转化为一种或多种产品的方法，其包括：用有效量的重组微生物的消耗的全发酵液处理植物细胞壁多糖，其中重组微生物表达一种或多种异源 编码将植物细胞壁多糖降解或转化成一种或多种产物的酶的基因。 本发明还涉及有机物质的制造方法，其特征在于，包括：（a）用有效量的重组微生物用的全部发酵肉汤糖化植物细胞壁多糖，其中所述重组微生物表达一种或多种编码酶的异源基因 其将植物细胞壁多糖降解或转化为糖化物质; （b）用一种或多种发酵微生物发酵步骤（a）的糖化物质; 和（c）从发酵中回收有机物质。
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公开(公告)号：US20160002591A1
公开(公告)日：2016-01-07
申请号：US14803808
申请日：2015-07-20
Applicant: NOVOZYMES INC.
Inventor： Randy Berka , Michael Rey , Preethi Ramaiya
Abstract: The present invention relates to isolated polynucleotides of the chromosome of Bacillus licheniformis SJ1904 that encode biologically active substances and to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing biologically active substances encoded by the polynucleotides and to methods of using the isolated polynucleotides of the complete chromosome of Bacillus licheniformis SJ1904.
Abstract translation: 本发明涉及编码生物活性物质的地衣芽孢杆菌SJ1904的染色体和包含多核苷酸的核酸构建体，载体和宿主细胞的分离的多核苷酸以及由多核苷酸编码的生物活性物质的制备方法， 使用地衣芽孢杆菌SJ1904完整染色体的分离多核苷酸。
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公开(公告)号：US20160289722A1
公开(公告)日：2016-10-06
申请号：US15183474
申请日：2016-06-15
Applicant: Novozymes, Inc.
Inventor： Randy Berka , Joel Cherry
CPC classification number: C12P19/14 , C12P5/023 , C12P7/00 , C12P7/02 , C12P7/04 , C12P7/06 , C12P7/10 , C12P7/16 , C12P7/18 , C12P7/20 , C12P7/26 , C12P7/28 , C12P7/40 , C12P13/04 , C12P17/04 , C12P19/02 , Y02E50/10 , Y02E50/16 , Y02E50/17 , Y02E50/343
Abstract: The present invention relates to methods for converting plant cell wall polysaccharides into one or more products, comprising: treating the plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant microorganism, wherein the recombinant microorganism expresses one or more heterologous genes encoding enzymes which degrade or convert the plant cell wall polysaccharides into the one or more products. The present invention also relates to methods for producing an organic substance, comprising: (a) saccharifying plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant microorganism, wherein the recombinant microorganism expresses one or more heterologous genes encoding enzymes which degrade or convert the plant cell wall polysaccharides into saccharified material; (b) fermenting the saccharified material of step (a) with one or more fermenting microoganisms; and (c) recovering the organic substance from the fermentation.
Abstract translation: 本发明涉及将植物细胞壁多糖转化为一种或多种产品的方法，其包括：用有效量的重组微生物的消耗的全发酵液处理植物细胞壁多糖，其中重组微生物表达一种或多种异源 编码将植物细胞壁多糖降解或转化成一种或多种产物的酶的基因。 本发明还涉及有机物质的制造方法，其特征在于，包括：（a）用有效量的重组微生物用的全部发酵肉汤糖化植物细胞壁多糖，其中所述重组微生物表达一种或多种编码酶的异源基因 其将植物细胞壁多糖降解或转化为糖化物质; （b）用一种或多种发酵微生物发酵步骤（a）的糖化物质; 和（c）从发酵中回收有机物质。
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公开(公告)号：US20130157309A1
公开(公告)日：2013-06-20
申请号：US13760763
申请日：2013-02-06
Applicant: NOVOZYMES, INC.
Inventor： Randy Berka , Michelle Maranta , Maria Tang , Barbara Cherry
IPC: C12N15/75
Abstract: The present invention relates to methods of obtaining genetic competence in non-competent Bacillus cells for their transformation with exogenous DNA.
Abstract translation: 本发明涉及在非感受态芽孢杆菌细胞中获得外源DNA转化的遗传能力的方法。
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