公开(公告)号：US08980073B2

公开(公告)日：2015-03-17

申请号：US13411221

申请日：2012-03-02

申请人： Nader Pourmand ,  Boaz Vilozny ,  Paolo Actis ,  R. Adam Seger ,  Bakthan Singaram

发明人： Nader Pourmand ,  Boaz Vilozny ,  Paolo Actis ,  R. Adam Seger ,  Bakthan Singaram

IPC分类号： G01N27/327 ,  G01N27/333 ,  B82Y35/00 ,  B01L3/02 ,  B01L3/00 ,  G01N33/487 ,  G01Q60/44

CPC分类号： G01N27/44791 ,  B01L3/021 ,  B01L3/50273 ,  B01L2300/0896 ,  B82Y35/00 ,  G01N33/48721 ,  G01Q60/44

摘要： Disclosed are methods and devices for detection of ion migration and binding, utilizing a nanopipette adapted for use in an electrochemical sensing circuit. The nanopipette may be functionalized on its interior bore with metal chelators for binding and sensing metal ions or other specific binding molecules such as boronic acid for binding and sensing glucose. Such a functionalized nanopipette is comprised in an electrical sensor that detects when the nanopipette selectively and reversibly binds ions or small molecules. Also disclosed is a nanoreactor, comprising a nanopipette, for controlling precipitation in aqueous solutions by voltage-directed ion migration, wherein ions may be directed out of the interior bore by a repulsing charge in the bore.

摘要翻译： 公开了用于检测离子迁移和结合的方法和装置，利用适用于电化学感测电路的纳米管。 可以在其内孔上用金属螯合剂官能化纳米针管，用于结合和感测金属离子或其它特异性结合分子，例如用于结合和感测葡萄糖的硼酸。 这种功能化的纳米移液管包含在电传感器中，该传感器检测纳米管片何时选择性地和可逆地结合离子或小分子。 还公开了一种纳米反应器，其包括纳米管，用于通过电压导向的离子迁移控制水溶液中的沉淀，其中离子可以通过孔中的排斥电荷引出到内孔内。

公开(公告)号：US20120222958A1

公开(公告)日：2012-09-06

申请号：US13411221

申请日：2012-03-02

申请人： Nader Pourmand ,  Boaz Vilozny ,  Paolo Actis ,  R. Adam Seger ,  Bakthan Singaram

发明人： Nader Pourmand ,  Boaz Vilozny ,  Paolo Actis ,  R. Adam Seger ,  Bakthan Singaram

IPC分类号： B01L3/02 ,  G01N27/00 ,  C25B7/00 ,  B82Y35/00 ,  B82Y30/00

CPC分类号： G01N27/44791 ,  B01L3/021 ,  B01L3/50273 ,  B01L2300/0896 ,  B82Y35/00 ,  G01N33/48721 ,  G01Q60/44

摘要： Disclosed are methods and devices for detection of ion migration and binding, utilizing a nanopipette adapted for use in an electrochemical sensing circuit. The nanopipette may be functionalized on its interior bore with metal chelators for binding and sensing metal ions or other specific binding molecules such as boronic acid for binding and sensing glucose. Such a functionalized nanopipette is comprised in an electrical sensor that detects when the nanopipette selectively and reversibly binds ions or small molecules. Also disclosed is a nanoreactor, comprising a nanopipette, for controlling precipitation in aqueous solutions by voltage-directed ion migration, wherein ions may be directed out of the interior bore by a repulsing charge in the bore.

摘要翻译： 公开了用于检测离子迁移和结合的方法和装置，利用适用于电化学感测电路的纳米管。 可以在其内孔上用金属螯合剂官能化纳米针管，用于结合和感测金属离子或其它特异性结合分子，例如用于结合和感测葡萄糖的硼酸。 这种功能化的纳米移液管包含在电传感器中，该传感器检测纳米管片何时选择性地和可逆地结合离子或小分子。 还公开了一种纳米反应器，其包括纳米管，用于通过电压导向的离子迁移控制水溶液中的沉淀，其中离子可以通过孔中的排斥电荷被引导出内孔。

公开(公告)号：US09598281B2

公开(公告)日：2017-03-21

申请号：US13406269

申请日：2012-02-27

申请人： R. Adam Seger ,  Paolo Actis ,  Boaz Vilozny ,  Nader Pourmand

发明人： R. Adam Seger ,  Paolo Actis ,  Boaz Vilozny ,  Nader Pourmand

IPC分类号： B82Y15/00 ,  B82Y5/00 ,  B01L3/00 ,  G01Q60/44 ,  G01N33/487

CPC分类号： B82Y5/00 ,  B01J2219/00371 ,  B01L3/502715 ,  B01L3/50273 ,  B01L2300/0645 ,  B82Y15/00 ,  G01N33/48728 ,  G01Q60/44

摘要： Disclosed herein are methods and systems for controlled ejection of desired material onto surfaces including in single cells using nanopipettes, as well as ejection onto and into cells. Some embodiments are directed to a method and system comprising nanopipettes combined with an xyz controller for depositing a user defined pattern on an arbitrary substrate for the purpose of controlled cell adhesion and growth. Alternate embodiments are directed to a method and system comprising nanopipettes combined with an xyz controller and electronic control of a voltage differential in a bore of the nanopipette electroosmotically injecting material into a cell in a high-throughput manner and with minimal damage to the cell. Yet other embodiments are directed to method and system comprising functionalized nanopipettes combined with scanning ion conductance microscopy for studying molecular interactions and detection of biomolecules inside a single living cell.

公开(公告)号：US20120225435A1

公开(公告)日：2012-09-06

申请号：US13406269

申请日：2012-02-27

申请人： R. Adam Seger ,  Paolo Actis ,  Boaz Vilozny ,  Nader Pourmand

发明人： R. Adam Seger ,  Paolo Actis ,  Boaz Vilozny ,  Nader Pourmand

IPC分类号： G01N33/53 ,  C12M1/34 ,  C12N5/071 ,  B82Y5/00 ,  B82Y99/00

CPC分类号： B82Y5/00 ,  B01J2219/00371 ,  B01L3/502715 ,  B01L3/50273 ,  B01L2300/0645 ,  B82Y15/00 ,  G01N33/48728 ,  G01Q60/44

摘要： Disclosed herein are methods and systems for controlled ejection of desired material onto surfaces including in single cells using nanopipettes, as well as ejection onto and into cells. Some embodiments are directed to a method and system comprising nanopipettes combined with an xyz controller for depositing a user defined pattern on an arbitrary substrate for the purpose of controlled cell adhesion and growth. Alternate embodiments are directed to a method and system comprising nanopipettes combined with an xyz controller and electronic control of a voltage differential in a bore of the nanopipette electroosmotically injecting material into a cell in a high-throughput manner and with minimal damage to the cell. Yet other embodiments are directed to method and system comprising functionalized nanopipettes combined with scanning ion conductance microscopy for studying molecular interactions and detection of biomolecules inside a single living cell.

摘要翻译： 本文公开了用于将所需材料控制性地喷射到包括在使用纳米移液管的单个细胞中的表面的方法和系统，以及喷射到细胞中并进入细胞。 一些实施例涉及包括与xyz控制器组合的纳米移液器的方法和系统，用于在任意基板上沉积用户限定的图案，以便控制细胞粘附和生长。 替代实施例涉及一种方法和系统，其包括与xyz控制器组合的纳米移液器和将纳米片的孔中的电压差的电子控制电子控制以高通量方式以高通量方式电动注入细胞并以最小的细胞损伤进行电子控制。 其他实施方案涉及包括与扫描离子电导显微镜结合的功能化纳米小片剂用于研究单个活细胞内的分子相互作用和生物分子的检测的方法和系统。