公开(公告)号：US20070240239A1

公开(公告)日：2007-10-11

申请号：US11765491

申请日：2007-06-20

申请人： Nadine Carozzi ,  Tracy Hargiss ,  Michael Koziel ,  Nicholas Duck ,  Brian Carr

发明人： Nadine Carozzi ,  Tracy Hargiss ,  Michael Koziel ,  Nicholas Duck ,  Brian Carr

IPC分类号： C12N15/63 ,  A01H1/00 ,  A01H5/00 ,  A01H7/00 ,  C12N5/04 ,  C12N5/14 ,  C12N15/82 ,  C07H21/04 ,  C12N1/20

CPC分类号： C07H21/04 ,  C07K14/325

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2 or 4, or the nucleotide sequence set forth in SEQ ID NO:1 or 3, as well as variants thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了包含编码SEQ ID NO：2或4所示的氨基酸序列的核苷酸序列或SEQ ID NO：1或3所示的核苷酸序列的分离的核酸分子及其变体 。

公开(公告)号：US20060242732A1

公开(公告)日：2006-10-26

申请号：US11396808

申请日：2006-04-03

申请人： Nadine Carozzi ,  Tracy Hargiss ,  Michael Koziel ,  Nicholas Duck ,  Theodore Kahn

发明人： Nadine Carozzi ,  Tracy Hargiss ,  Michael Koziel ,  Nicholas Duck ,  Theodore Kahn

IPC分类号： A01H1/00 ,  C07H21/04 ,  C12N15/82 ,  C12N5/04 ,  C12N1/21 ,  C07K14/325

CPC分类号： C07K14/325 ,  A01N63/02 ,  C12N15/8286 ,  Y02A40/162

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NOS:2, 11 and 13, or the nucleotide sequences set forth in SEQ ID NOS:1, 10 and 12, as well as variants and fragments thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：2,11和13所示的氨基酸序列的核苷酸序列或SEQ ID NO：1,10和12所示的核苷酸序列，作为 以及其变体和片段。

公开(公告)号：US20060212965A1

公开(公告)日：2006-09-21

申请号：US11343533

申请日：2006-01-31

申请人： Nadine Carozzi ,  Tracy Hargiss ,  Michael Koziel ,  Nicholas Duck

发明人： Nadine Carozzi ,  Tracy Hargiss ,  Michael Koziel ,  Nicholas Duck

IPC分类号： A01H1/00 ,  C12N15/82 ,  A01H5/00 ,  C12N5/04 ,  A01N63/00 ,  C07K14/415

CPC分类号： C07K14/325 ,  A01N63/02 ,  C12N15/8281 ,  C12N15/8285 ,  C12N15/8286 ,  Y02A40/162 ,  Y02A40/164

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, or 6, or the nucleotide sequence set forth in SEQ ID NO:1, 3, or 5, as well as variants and fragments thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：2,4或6所示氨基酸序列的核苷酸序列或SEQ ID NO：1,3或5所示的核苷酸序列 ，以及其变体和片段。

公开(公告)号：US20080070829A1

公开(公告)日：2008-03-20

申请号：US11763947

申请日：2007-06-15

申请人： Nadine Carozzi ,  Michael Koziel ,  Nicholas Duck ,  Nalini Desai ,  Rong Guo ,  Daniel Tomso ,  Rebekah Deter ,  Tracy Hargiss

发明人： Nadine Carozzi ,  Michael Koziel ,  Nicholas Duck ,  Nalini Desai ,  Rong Guo ,  Daniel Tomso ,  Rebekah Deter ,  Tracy Hargiss

IPC分类号： A01N37/18 ,  A01H1/00 ,  A01H5/00 ,  A01P5/00 ,  C12N1/00 ,  C12N15/63 ,  C12P21/00 ,  C12N5/04 ,  C12N1/21 ,  C07H21/00 ,  A01P15/00 ,  A01H5/10

CPC分类号： C12N15/8286 ,  A01N37/44 ,  C07K14/325 ,  C12P21/02 ,  Y02A40/162

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 6, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37 or 61, the nucleotide sequence set forth in SEQ ID NO:1, 3, 5, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, or 60, or the nucleotide sequence deposited in a bacterial host as Accession No. NRRL B-30961, B-30955, B-30956, B-30957, B-30958, B-30942, B-30939, B-30941, B-50047, B-50047, B-30959, B-30960, B-30943, B-50048, or B-50048, as well as variants and fragments thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含杀虫多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的杀虫核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO： 25，27，29，31，33，35，37或61所述的核苷酸序列，所述核苷酸序列如SEQ ID NO：1,3,5,8,10,12,14,16,18,20,22,24， 26,28,30,32,34,36或60个核苷酸序列，或作为保藏号NRRL B-30961，B-30955，B-30956，B-30957，B-30958，B -30942，B-30939，B-30941，B-50047，B-50047，B-30959，B-30960，B-30943，B-50048或B-50048，以及其变体和片段。

公开(公告)号：US20070238646A1

公开(公告)日：2007-10-11

申请号：US11765494

申请日：2007-06-20

申请人： Nadine Carozzi ,  Tracy Hargiss ,  Michael Koziel ,  Nicholas Duck ,  Brian Carr

发明人： Nadine Carozzi ,  Tracy Hargiss ,  Michael Koziel ,  Nicholas Duck ,  Brian Carr

IPC分类号： A61K38/00 ,  C07K1/00

CPC分类号： C07H21/04 ,  C07K14/325

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2 or 4, or the nucleotide sequence set forth in SEQ ID NO:1 or 3, as well as variants thereof

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了包含编码SEQ ID NO：2或4所示的氨基酸序列的核苷酸序列或SEQ ID NO：1或3所示的核苷酸序列的分离的核酸分子及其变体

公开(公告)号：US20070044178A1

公开(公告)日：2007-02-22

申请号：US11416261

申请日：2006-05-02

申请人： Nadine Carozzi ,  Michael Koziel ,  Tracy Hargiss ,  Nicholas Duck ,  Theodore Kahn

发明人： Nadine Carozzi ,  Michael Koziel ,  Tracy Hargiss ,  Nicholas Duck ,  Theodore Kahn

IPC分类号： A01H5/00 ,  C07H21/04 ,  C12N15/82 ,  C12N5/04 ,  A01N65/00 ,  C07K14/415 ,  A01H1/00

CPC分类号： C07K14/325 ,  C12N15/8286 ,  Y02A40/162

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 15, 17, or 19, or the nucleotide sequence set forth in SEQ ID NO:1, 3, 14, 16, or 18, as well as variants and fragments thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：2,4,15,17或19所示氨基酸序列的核苷酸序列或SEQ ID NO：1所示的核苷酸序列， 3,14,16或18，以及其变体和片段。

公开(公告)号：US20050183161A1

公开(公告)日：2005-08-18

申请号：US10958008

申请日：2004-10-04

申请人： Nadine Carozzi ,  Tracy Hargiss ,  Michael Koziel ,  Nicholas Duck ,  Brian Carr

发明人： Nadine Carozzi ,  Tracy Hargiss ,  Michael Koziel ,  Nicholas Duck ,  Brian Carr

IPC分类号： C07K14/325 ,  C12N15/82 ,  A01H1/00 ,  A01N63/00 ,  C12Q1/68 ,  C07H21/04

CPC分类号： C12N15/8286 ,  C07K14/325 ,  Y02A40/162

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, or the nucleotide sequence set forth in SEQ ID NO: 1, as well as variants thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了包含编码SEQ ID NO：2所示的氨基酸序列的核苷酸序列或SEQ ID NO：1所示的核苷酸序列的分离的核酸分子及其变体。

公开(公告)号：US20050049410A1

公开(公告)日：2005-03-03

申请号：US10926819

申请日：2004-08-26

申请人： Nadine Carozzi ,  Tracy Hargiss ,  Michael Koziel ,  Nicholas Duck ,  Brian Carr

发明人： Nadine Carozzi ,  Tracy Hargiss ,  Michael Koziel ,  Nicholas Duck ,  Brian Carr

IPC分类号： A01N63/02 ,  C07H21/04 ,  C07K14/325 ,  C12N15/82 ,  C12N1/20

CPC分类号： C07H21/04 ,  C07K14/325

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2 or 4, or the nucleotide sequence set forth in SEQ ID NO:1 or 3, as well as variants thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了包含编码SEQ ID NO：2或4所示的氨基酸序列的核苷酸序列或SEQ ID NO：1或3所示的核苷酸序列的分离的核酸分子及其变体 。

公开(公告)号：US20140344999A1

公开(公告)日：2014-11-20

申请号：US14451659

申请日：2014-08-05

申请人： Nadine Carozzi ,  Michael G. Koziel ,  Nicholas Duck ,  Nalini M. Desai ,  Rong Guo ,  Daniel John Tomso ,  Rebekah Deter ,  Tracy Hargiss

发明人： Nadine Carozzi ,  Michael G. Koziel ,  Nicholas Duck ,  Nalini M. Desai ,  Rong Guo ,  Daniel John Tomso ,  Rebekah Deter ,  Tracy Hargiss

IPC分类号： C12N15/82 ,  C07K14/325 ,  C12P21/02 ,  A01N37/44

CPC分类号： C12N15/8286 ,  A01N37/44 ,  C07K14/325 ,  C12P21/02 ,  Y02A40/162

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO: 2, 4, 6, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37 or 61, the nucleotide sequence set forth in SEQ ID NO: 1, 3, 5, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, or 60, or the nucleotide sequence deposited in a bacterial host as Accession No. B-30955, B-30956, B-30957, B-30958, B-30942, B-30939, B-30941, B-50047, B-30959, B-30960, B-30943, or B-50048, as well as variants and fragments thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含杀虫多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的杀虫核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO： 25，27，29，31，33，35，37或61所述的核苷酸序列，所述核苷酸序列如SEQ ID NO：1,3,5,8,10,12,14,16,18,20,22,24， 26,28,30,32,34,36或60个核苷酸序列，或作为保藏号B-30955，B-30956，B-30957，B-30958，B-30942，B- 30939，B-30941，B-50047，B-30959，B-30960，B-30943或B-50048，以及其变体和片段。

公开(公告)号：US08829279B2

公开(公告)日：2014-09-09

申请号：US13168454

申请日：2011-06-24

申请人： Nadine Carozzi ,  Michael G. Koziel ,  Nicholas Duck ,  Nalini M. Desai ,  Rong Guo ,  Daniel John Tomso ,  Rebekah Deter ,  Tracy Hargiss

发明人： Nadine Carozzi ,  Michael G. Koziel ,  Nicholas Duck ,  Nalini M. Desai ,  Rong Guo ,  Daniel John Tomso ,  Rebekah Deter ,  Tracy Hargiss

IPC分类号： A01H5/00 ,  C12N15/82 ,  C07K14/325 ,  A01N37/18

CPC分类号： C12N15/8286 ,  A01N37/44 ,  C07K14/325 ,  C12P21/02 ,  Y02A40/162

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 6, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37 or 61, the nucleotide sequence set forth in SEQ ID NO:1, 3, 5, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, or 60, or the nucleotide sequence deposited in a bacterial host as Accession No. NRRL B-30961, B-30955, B-30956, B-30957, B-30958, B-30942, B-30939, B-30941, B-50047, B-30959, B-30960, B-30943, or B-50048, as well as variants and fragments thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含杀虫多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的杀虫核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO： 25，27，29，31，33，35，37或61所述的核苷酸序列，所述核苷酸序列如SEQ ID NO：1,3,5,8,10,12,14,16,18,20,22,24， 26,28,30,32,34,36或60个核苷酸序列，或作为保藏号NRRL B-30961，B-30955，B-30956，B-30957，B-30958，B -30942，B-30939，B-30941，B-50047，B-30959，B-30960，B-30943或B-50048，以及其变体和片段。