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    Isolating Cells Expressing Secreted Proteins
    2.
    发明申请
    Isolating Cells Expressing Secreted Proteins 审中-公开
    分离表达分泌蛋白的细胞

    公开(公告)号：US20090137416A1

    公开(公告)日：2009-05-28

    申请号：US12240541

    申请日：2008-09-29

    申请人： James P. Fandl ,  Gang Chen ,  Neil Stahl ,  George D. Yancopoulos

    发明人： James P. Fandl ,  Gang Chen ,  Neil Stahl ,  George D. Yancopoulos

    IPC分类号： C40B30/04 ,  G01N33/53

    CPC分类号： G01N33/6854 ,  C12N15/65 ,  G01N33/566 ,  G01N33/56966 ,  G01N2333/7051

    摘要： A method of detecting and isolating cells that produce a secreted protein of interest (POI) that has a T cell receptor variable domain, comprising: a) constructing a cell line transiently or stably expressing a cell surface capture molecule, which binds the POI, by transfecting the cell line with a nucleic acid that encodes such cell surface capture molecule; b) transfecting said cell simultaneously or subsequently with a second nucleic acid that encodes a POI wherein such POI is secreted; c) detecting the surface-displayed POI by contacting the cells with a detection molecule, which binds the POI; and d) isolating cells based on the detection molecule.

    摘要翻译： 一种检测和分离产生具有T细胞受体可变区的目标分泌蛋白质（POI）的细胞的方法，包括：a）通过以下步骤构建细胞系：瞬时或稳定表达结合POI的细胞表面捕获分子，通过 用编码这种细胞表面捕获分子的核酸转染细胞系; b）同时或随后用编码POI的第二核酸同时转染所述细胞，其中所述POI被分泌; c）通过使细胞与结合POI的检测分子接触来检测表面显示的POI; 和d）基于检测分子分离细胞。

    Methods of treating anemia by inhibition of vascular endothelial growth factor (VEGF)
    4.
    发明申请
    Methods of treating anemia by inhibition of vascular endothelial growth factor (VEGF) 有权
    通过抑制血管内皮生长因子（VEGF）治疗贫血的方法

    公开(公告)号：US20060210566A1

    公开(公告)日：2006-09-21

    申请号：US11373358

    申请日：2006-03-10

    申请人： Jocelyn Holash ,  Jesse Cedarbaum ,  John Rudge ,  George Yancopoulos ,  Neil Stahl

    发明人： Jocelyn Holash ,  Jesse Cedarbaum ,  John Rudge ,  George Yancopoulos ,  Neil Stahl

    IPC分类号： A61K39/395

    CPC分类号： C07K14/71 ,  A61K38/00 ,  C07K2319/30

    摘要： Methods of treating anemic disorders in human subjects comprising administering a VEGF antagonist comprising a fusion polypeptide having an immunoglobulin-like (Ig) domain 2 of the VEGF receptor Flt1 and Ig domain 3 of the VEGF receptor Flk1 or Flt4, and a multimerizing component. Further included are methods of preventing anemia, increasing hematocrit levels, and increasing or stimulating erythropoietin, particularly in subjects being treated for cancer with chemotherapeutic agents and/or radiation.

    摘要翻译： 治疗人受试者中贫血障碍的方法包括给予包含融合多肽的VEGF拮抗剂，所述融合多肽具有VEGF受体Flt1的免疫球蛋白样（Ig）结构域2和VEGF受体Flk1或Flt4的Ig结构域3和多聚化成分。 还包括预防贫血，增加血细胞比容水平以及增加或刺激促红细胞生成素的方法，特别是在用化学治疗剂和/或辐射治疗癌症的受试者中。

    Methods of treating diseases with a VEGF antagonist
    7.
    发明授权
    Methods of treating diseases with a VEGF antagonist 有权
    用VEGF拮抗剂治疗疾病的方法

    公开(公告)号：US07531172B2

    公开(公告)日：2009-05-12

    申请号：US11502736

    申请日：2006-08-11

    申请人： Neil Stahl ,  George D. Yancopoulos ,  Eric Furfine ,  Jesse M. Cedarbaum

    发明人： Neil Stahl ,  George D. Yancopoulos ,  Eric Furfine ,  Jesse M. Cedarbaum

    IPC分类号： A61K38/18 ,  C07K14/71 ,  C12N15/62

    CPC分类号： A61K38/179

    摘要： A method of reducing or preventing hypertension associated with administration of a vascular endothelial growth factor (VEGF) antagonist in a human subjects suffering from a disease or condition treatable with a VEGF antagonist in which is it desirable to reduce or prevent hypertension. The method is particularly useful for treatment of patients unresponsive to treatment with a VEGF inhibitor administered intravenously.

    摘要翻译： 在患有可用VEGF拮抗剂治疗的疾病或病症的人类受试者中降低或预防与血管内皮生长因子（VEGF）拮抗剂的给药相关的高血压的方法，其中希望减少或预防高血压。 该方法对于治疗对用静脉内施用的VEGF抑制剂治疗无反应的患者特别有用。

    Methods of using cytokine antagonists to treat HIV infection and AIDS
    8.
    发明授权
    Methods of using cytokine antagonists to treat HIV infection and AIDS 有权
    使用细胞因子拮抗剂治疗艾滋病毒和艾滋病的方法

    公开(公告)号：US06811780B2

    公开(公告)日：2004-11-02

    申请号：US10427863

    申请日：2003-05-01

    申请人： Eric S. Furfine ,  Neil Stahl

    发明人： Eric S. Furfine ,  Neil Stahl

    IPC分类号： A61K39395

    CPC分类号： C07K16/247 ,  A61K38/1793 ,  A61K39/39 ,  A61K2039/505 ,  A61K2039/55516 ,  A61K2039/57

    摘要： The invention is methods of using cytokine antagonists to treat HIV. In particular, the invention is methods of treating HIV using L-4 and IL-13 cytokine antagonists, including cytokine traps and anti-IL-4 or anti-IL-13 antibodies. The invention also includes augmenting the efficacy of HIV vaccination by co-administration of the vaccine with IL-4 and IL-13 cytokine antagonists.

    摘要翻译： 本发明是使用细胞因子拮抗剂治疗HIV的方法。 特别地，本发明是使用L-4和IL-13细胞因子拮抗剂（包括细胞因子捕获物和抗IL-4或抗IL-13抗体）来治疗HIV的方法。 本发明还包括通过将疫苗与IL-4和IL-13细胞因子拮抗剂共同给药来增强HIV疫苗接种的功效。

    DCR5, a BMP-binding protein, and applications thereof
    9.
    发明授权
    DCR5, a BMP-binding protein, and applications thereof 有权
    DCR5，BMP结合蛋白及其应用

    公开(公告)号：US06660499B1

    公开(公告)日：2003-12-09

    申请号：US09762960

    申请日：2001-02-14

    申请人： Aris N. Economides ,  Neil Stahl

    发明人： Aris N. Economides ,  Neil Stahl

    IPC分类号： C12P2106

    CPC分类号： C07K14/475 ,  A61K38/00 ,  C07K2319/02

    摘要： DCR5, a protein related to DAN (Differential-screening-selected gene Aberrative in Neuroblastoma) and related nucleic acids are provided. Included are natural DCR5 homologs from several species and proteins comprising a DCR5 domain having specific activity, particularly the ability to antagonize a bone morphogenetic protein. The proteins may be produced recombinantly from transformed host cells with the subject nucleic acids. Also provided are isolated hybridization probes and primers capable of specifically hybridizing with the disclosed genes, specific binding agents and methods of making and using the subject compositions.

    摘要翻译： 提供了与DAN相关的蛋白质（神经母细胞瘤中的差异筛选选择基因Aberrative）和相关核酸的DCR5。 包括来自几种物种的天然DCR5同源物和包含具有比活性的DCR5结构域的蛋白质，特别是拮抗骨形态发生蛋白的能力。 蛋白质可以用受试核酸从转化的宿主细胞重组产生。 还提供了能够与所公开的基因，特异性结合剂和制备和使用本发明组合物的方法特异性杂交的分离的杂交探针和引物。

    Isolating cells expressing secreted proteins
    10.
    发明授权
    Isolating cells expressing secreted proteins 有权
    分离表达分泌蛋白的细胞

    公开(公告)号：US07435553B2

    公开(公告)日：2008-10-14

    申请号：US11434403

    申请日：2006-05-15

    申请人： James P Fandl ,  Neil Stahl ,  Gang Chen ,  George D. Yancopoulos

    发明人： James P Fandl ,  Neil Stahl ,  Gang Chen ,  George D. Yancopoulos

    IPC分类号： G01N33/53 ,  C12N15/09

    CPC分类号： C40B30/04 ,  A01K2217/05 ,  A01K2267/01 ,  C07K14/315 ,  C07K14/52 ,  C07K16/2863 ,  C07K2317/77 ,  C07K2319/00 ,  C12N15/1051 ,  G01N33/56966 ,  G01N33/6845 ,  G01N2500/10

    摘要： A method of detecting and isolating cells that produce any secreted protein of interest (POI), comprising: a) constructing a cell line transiently or stably expressing a cell surface capture molecule, which binds the POI, by transfecting the cell line with a nucleic acid that encodes such cell surface capture molecule; b) transfecting said cell simultaneously or subsequently with a second nucleic acid that encodes a POI wherein such POI is secreted; c) detecting the surface-displayed POI by contacting the cells with a detection molecule, which binds the POI; and d) isolating cells based on the detection molecule.

    摘要翻译： 一种检测和分离产生任何分泌的感兴趣蛋白质（POI）的细胞的方法，包括：a）通过用核酸转染细胞系来构建瞬时或稳定表达结合POI的细胞表面捕获分子的细胞系 编码这种细胞表面捕获分子; b）同时或随后用编码POI的第二核酸同时转染所述细胞，其中所述POI被分泌; c）通过使细胞与结合POI的检测分子接触来检测表面显示的POI; 和d）基于检测分子分离细胞。