利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

21 条记录 (耗时 0.02 秒)

    Method for detecting a target nucleic acid sequence
    2.
    发明授权
    Method for detecting a target nucleic acid sequence 有权
    检测靶核酸序列的方法

    公开(公告)号：US07919252B2

    公开(公告)日：2011-04-05

    申请号：US12341295

    申请日：2008-12-22

    申请人： Naoko Nakamura ,  Koji Hashimoto

    发明人： Naoko Nakamura ,  Koji Hashimoto

    IPC分类号： C12Q1/68 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12Q1/6813

    摘要： A method of detecting a target nucleic acid sequence comprising providing a stem-and-loop structured nucleic acid for measurement wherein the nucleic acid comprises complementary sequence portions located at both terminals and a target sequence portion therebetween as well as a double-stranded portion formed by hybridization of the complementary sequence portions located at both terminals and a remaining looped single-stranded portion, providing a probe nucleic acid having a sequence complementary to the target sequence portion wherein one end of the probe nucleic acid being immobilized to a solid substrate surface, reacting the nucleic acid for measurement with the probe nucleic acid to specifically hybridize the target sequence portion of the nucleic acid for measurement to the probe nucleic acid, and detecting presence or absence of the nucleic acid for measurement hybridized to the probe nucleic acid.

    摘要翻译： 一种检测靶核酸序列的方法，包括提供用于测量的茎 - 环结构化核酸，其中所述核酸包含位于两端的互补序列部分和其间的靶序列部分以及由双链部分形成的双链部分 位于两个末端的互补序列部分和剩余的环状单链部分的杂交，提供具有与靶序列部分互补的序列的探针核酸，其中探针核酸的一端被固定在固体基质表面上，使 用于与探针核酸进行测量的核酸以将用于测量的核酸的靶序列部分特异性杂交到探针核酸，并检测与探针核酸杂交的用于测量的核酸的存在或不存在。

    Sample analysis method and assay kit used therein
    3.
    发明授权
    Sample analysis method and assay kit used therein 失效
    其中使用的样品分析方法和测定试剂盒

    公开(公告)号：US08673595B2

    公开(公告)日：2014-03-18

    申请号：US13340572

    申请日：2011-12-29

    申请人： Naoko Nakamura ,  Koji Hashimoto ,  Nobuhiro Gemma

    发明人： Naoko Nakamura ,  Koji Hashimoto ,  Nobuhiro Gemma

    IPC分类号： C12P19/34

    CPC分类号： C12Q1/6837 ,  C12Q1/6809 ,  C12Q1/6853 ,  C12Q2525/161 ,  C12Q2525/301 ,  C12Q2537/143 ,  C12Q2563/179 ,  C12Q2565/514

    摘要： One embodiment is related to a method of analyzing plural samples. The method includes amplifying a plurality of samples using a first primer and second primer, wherein the first primer includes a tag sequence having a sequence different from a sample to one another and wherein a second primer used in pair with the first primer in independent reaction systems for the respective samples to obtain an amplified product in which the tag sequence is introduced, mixing amplified products obtained in the plurality of reaction systems, making the mixed amplified product react with a nucleic acid probe immobilized on a substrate, and detecting the amount of hybridization that has occurred.

    摘要翻译： 一个实施例涉及分析多个样本的方法。 该方法包括使用第一引物和第二引物扩增多个样品，其中第一引物包含具有不同于样品的序列的标签序列，并且其中与独立反应体系中的第一引物成对配对的第二引物 对于各样品获得其中引入标签序列的扩增产物，混合在多个反应体系中获得的扩增产物，使得混合的扩增产物与固定在底物上的核酸探针反应，并检测杂交的量 已经发生了。

    METHOD OF DETECTING TARGET NUCLEIC ACID
    6.
    发明申请
    METHOD OF DETECTING TARGET NUCLEIC ACID 审中-公开
    检测目标核酸的方法

    公开(公告)号：US20090275028A1

    公开(公告)日：2009-11-05

    申请号：US12329898

    申请日：2008-12-08

    申请人： Naoko Nakamura ,  Koji Hashimoto ,  Nobuhiro Gemma

    发明人： Naoko Nakamura ,  Koji Hashimoto ,  Nobuhiro Gemma

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6832 ,  C12Q1/6837 ,  C12Q2527/101 ,  C12Q2525/204 ,  C12Q2565/501

    摘要： The present invention provides a method of detecting a target nucleic acid which includes a step of examining whether a washing step has been normally conducted. In an aspect of the invention, a monitoring nucleic acid probe to monitor the washing level is used. The probe shows a change in signal intensity by washing at a washing temperature changed in the optimum temperature range for washing the target nucleic acid and in a temperature range in the vicinity of the optimum temperature range for washing.

    摘要翻译： 本发明提供了检测目标核酸的方法，其包括检查洗涤步骤是否正常进行的步骤。 在本发明的一个方面，使用监测洗涤水平的监测核酸探针。 探针通过在洗涤目标核酸的最适温度范围内和在用于洗涤的最佳温度范围附近的温度范围内改变的洗涤温度下洗涤来显示信号强度的变化。

    Method for detecting a target nucleic acid sequence
    7.
    发明授权
    Method for detecting a target nucleic acid sequence 有权
    检测靶核酸序列的方法

    公开(公告)号：US07488581B2

    公开(公告)日：2009-02-10

    申请号：US11378453

    申请日：2006-03-20

    申请人： Naoko Nakamura ,  Koji Hashimoto

    发明人： Naoko Nakamura ,  Koji Hashimoto

    IPC分类号： C12Q1/68 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12Q1/6813

    摘要： A method of detecting a target nucleic acid sequence comprising providing a stem-and-loop structured nucleic acid for measurement wherein the nucleic acid comprises complementary sequence portions located at both terminals and a target sequence portion therebetween as well as a double-stranded portion formed by hybridization of the complementary sequence portions located at both terminals and a remaining looped single-stranded portion, providing a probe nucleic acid having a sequence complementary to the target sequence portion wherein one end of the probe nucleic acid being immobilized to a solid substrate surface, reacting the nucleic acid for measurement with the probe nucleic acid to specifically hybridize the target sequence portion of the nucleic acid for measurement to the probe nucleic acid, and detecting presence or absence of the nucleic acid for measurement hybridized to the probe nucleic acid.

    摘要翻译： 一种检测靶核酸序列的方法，包括提供用于测量的茎 - 环结构化核酸，其中所述核酸包含位于两端的互补序列部分和其间的靶序列部分以及由双链部分形成的双链部分 位于两个末端的互补序列部分和剩余的环状单链部分的杂交，提供具有与靶序列部分互补的序列的探针核酸，其中探针核酸的一端被固定在固体基质表面上，使 用于与探针核酸进行测量的核酸以将用于测量的核酸的靶序列部分特异性杂交到探针核酸，并检测与探针核酸杂交的用于测量的核酸的存在或不存在。

    Method for detecting a target nucleic acid sequence
    8.
    发明申请
    Method for detecting a target nucleic acid sequence 有权
    检测靶核酸序列的方法

    公开(公告)号：US20070218464A1

    公开(公告)日：2007-09-20

    申请号：US11378453

    申请日：2006-03-20

    申请人： Naoko Nakamura ,  Koji Hashimoto

    发明人： Naoko Nakamura ,  Koji Hashimoto

    IPC分类号： C12Q1/68 ,  C12M3/00

    CPC分类号： C12Q1/6813

    摘要： A method of detecting a target nucleic acid sequence comprising providing a stem-and-loop structured nucleic acid for measurement wherein the nucleic acid comprises complementary sequence portions located at both terminals and a target sequence portion therebetween as well as a double-stranded portion formed by hybridization of the complementary sequence portions located at both terminals and a remaining looped single-stranded portion, providing a probe nucleic acid having a sequence complementary to the target sequence portion wherein one end of the probe nucleic acid being immobilized to a solid substrate surface, reacting the nucleic acid for measurement with the probe nucleic acid to specifically hybridize the target sequence portion of the nucleic acid for measurement to the probe nucleic acid, and detecting presence or absence of the nucleic acid for measurement hybridized to the probe nucleic acid.

    摘要翻译： 一种检测靶核酸序列的方法，包括提供用于测量的茎 - 环结构化核酸，其中所述核酸包含位于两端的互补序列部分和其间的靶序列部分以及由双链部分形成的双链部分 位于两个末端的互补序列部分和剩余的环状单链部分的杂交，提供具有与靶序列部分互补的序列的探针核酸，其中探针核酸的一端被固定在固体基质表面上，使 用于与探针核酸进行测量的核酸以将用于测量的核酸的靶序列部分特异性杂交到探针核酸，并检测与探针核酸杂交的用于测量的核酸的存在或不存在。

    Nucleotide primer set and nucleotide probe for detecting genotype of N-acetyltransferase-2 (NAT2)
    9.
    发明授权
    Nucleotide primer set and nucleotide probe for detecting genotype of N-acetyltransferase-2 (NAT2) 失效
    用于检测N-乙酰转移酶-2（NAT2）基因型的核苷酸引物组和核苷酸探针

    公开(公告)号：US07919611B2

    公开(公告)日：2011-04-05

    申请号：US12014592

    申请日：2008-01-15

    申请人： Naoko Nakamura ,  Keiko Ito ,  Koji Hashimoto ,  Nobuhiro Gemma

    发明人： Naoko Nakamura ,  Keiko Ito ,  Koji Hashimoto ,  Nobuhiro Gemma

    IPC分类号： C07H21/04 ,  C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6883 ,  C12Q2600/156

    摘要： There is provided a nucleotide primer set for LAMP amplification, used for detecting genotypes of single-nucleotide polymorphisms G590A, G857A and T341C of a NAT2 gene. There is also provided a nucleotide probe for detection of an amplification product amplified with the primer set according to the present invention. There is also provided a method of detecting the genotypes of NAT2 gene single-nucleotide polymorphisms G590A, G857A and T341C by using the primer set according to the present invention.

    摘要翻译： 提供了LAMP扩增的核苷酸引物组，用于检测NAT2基因的单核苷酸多态性G590A，G857A和T341C的基因型。 还提供了用于检测根据本发明的引物组扩增的扩增产物的核苷酸探针。 还提供了通过使用本发明的引物组来检测NAT2基因单核苷酸多态性G590A，G857A和T341C的基因型的方法。

    NUCLEOTIDE PRIMER SET AND NUCLEOTIDE PROBE FOR DETECTING GENOTYPE OF N-ACETYLTRANSFERASE-2 (NAT2)
    10.
    发明申请
    NUCLEOTIDE PRIMER SET AND NUCLEOTIDE PROBE FOR DETECTING GENOTYPE OF N-ACETYLTRANSFERASE-2 (NAT2) 失效
    用于检测N-乙酰基转移酶-2（NAT2）基因组的核酸酶基因组和核酸探针

    公开(公告)号：US20100003671A1

    公开(公告)日：2010-01-07

    申请号：US12014592

    申请日：2008-01-15

    申请人： Naoko Nakamura ,  Keiko Ito ,  Koji Hashimoto ,  Nobuhiro Gemma

    发明人： Naoko Nakamura ,  Keiko Ito ,  Koji Hashimoto ,  Nobuhiro Gemma

    IPC分类号： C12Q1/68 ,  C07H21/00

    CPC分类号： C12Q1/6883 ,  C12Q2600/156

    摘要： There is provided a nucleotide primer set for LAMP amplification, used for detecting genotypes of single-nucleotide polymorphisms G590A, G857A and T341C of a NAT2 gene. There is also provided a nucleotide probe for detection of an amplification product amplified with the primer set according to the present invention. There is also provided a method of detecting the genotypes of NAT2 gene single-nucleotide polymorphisms G590A, G857A and T341C by using the primer set according to the present invention.

    摘要翻译： 提供了LAMP扩增的核苷酸引物组，用于检测NAT2基因的单核苷酸多态性G590A，G857A和T341C的基因型。 还提供了用于检测根据本发明的引物组扩增的扩增产物的核苷酸探针。 还提供了通过使用本发明的引物组来检测NAT2基因单核苷酸多态性G590A，G857A和T341C的基因型的方法。