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    METHOD OF DESIGNING PRIMERS FOR USE IN METHOD OF DETECTING TARGET NUCLEIC ACID AND ASSAY KIT
    1.
    发明申请
    METHOD OF DESIGNING PRIMERS FOR USE IN METHOD OF DETECTING TARGET NUCLEIC ACID AND ASSAY KIT 有权
    设计用于检测目标核酸和测定试剂盒的方法中使用的方法

    公开(公告)号：US20110021379A1

    公开(公告)日：2011-01-27

    申请号：US12851675

    申请日：2010-08-06

    申请人： Naoko NAKAMURA ,  Keiko Ito

    发明人： Naoko NAKAMURA ,  Keiko Ito

    IPC分类号： C40B40/06 ,  C12Q1/68

    CPC分类号： C12Q1/6844 ,  C12Q2525/161 ,  C12Q2525/301 ,  C12Q2531/101

    摘要： Kits for use in a method of detecting an amplification product by hybridizing it with a probe, the amplification product is amplified from a target nucleic acid with the primers, including placing F3, F2 and F1 regions in this order from a 5′ terminal side and B3c, B2c and B1c regions in this order from a 3′ terminal side, and additionally an FP region in the region from the F2 to F1 regions and/or a BPc region in the region from the B2c to B1c regions in the target nucleic acid, determining the respective regions in such a manner that the FP and F2 regions and/or the BPc and B2c regions have an unoverlapping region of at least 10 bases or more and overlapping regions of 10 bases or less, and designing the primers according to the regions.

    摘要翻译： 用于通过与探针杂交来检测扩增产物的方法的试剂盒，使用引物从靶核酸扩增扩增产物，包括从5'末端侧依次放置F3，F2和F1区， B3c，B2c和B1c区域，另外在靶核酸中的从B2c到B1c区域的F2到F1区和/或BPc区的区域中的FP区域 以这样的方式确定各个区域，使得FP和F2区域和/或BPc和B2c区域具有至少10个碱基以上的重叠区域和10个碱基以下的重叠区域，并且根据 地区。

    Nucleotide primer set and nucleotide probe for detecting genotype of N-acetyltransferase-2 (NAT2)
    3.
    发明授权
    Nucleotide primer set and nucleotide probe for detecting genotype of N-acetyltransferase-2 (NAT2) 失效
    用于检测N-乙酰转移酶-2（NAT2）基因型的核苷酸引物组和核苷酸探针

    公开(公告)号：US07919611B2

    公开(公告)日：2011-04-05

    申请号：US12014592

    申请日：2008-01-15

    申请人： Naoko Nakamura ,  Keiko Ito ,  Koji Hashimoto ,  Nobuhiro Gemma

    发明人： Naoko Nakamura ,  Keiko Ito ,  Koji Hashimoto ,  Nobuhiro Gemma

    IPC分类号： C07H21/04 ,  C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6883 ,  C12Q2600/156

    摘要： There is provided a nucleotide primer set for LAMP amplification, used for detecting genotypes of single-nucleotide polymorphisms G590A, G857A and T341C of a NAT2 gene. There is also provided a nucleotide probe for detection of an amplification product amplified with the primer set according to the present invention. There is also provided a method of detecting the genotypes of NAT2 gene single-nucleotide polymorphisms G590A, G857A and T341C by using the primer set according to the present invention.

    摘要翻译： 提供了LAMP扩增的核苷酸引物组，用于检测NAT2基因的单核苷酸多态性G590A，G857A和T341C的基因型。 还提供了用于检测根据本发明的引物组扩增的扩增产物的核苷酸探针。 还提供了通过使用本发明的引物组来检测NAT2基因单核苷酸多态性G590A，G857A和T341C的基因型的方法。

    NUCLEOTIDE PRIMER SET AND NUCLEOTIDE PROBE FOR DETECTING GENOTYPE OF N-ACETYLTRANSFERASE-2 (NAT2)
    5.
    发明申请
    NUCLEOTIDE PRIMER SET AND NUCLEOTIDE PROBE FOR DETECTING GENOTYPE OF N-ACETYLTRANSFERASE-2 (NAT2) 失效
    用于检测N-乙酰基转移酶-2（NAT2）基因组的核酸酶基因组和核酸探针

    公开(公告)号：US20100003671A1

    公开(公告)日：2010-01-07

    申请号：US12014592

    申请日：2008-01-15

    申请人： Naoko Nakamura ,  Keiko Ito ,  Koji Hashimoto ,  Nobuhiro Gemma

    发明人： Naoko Nakamura ,  Keiko Ito ,  Koji Hashimoto ,  Nobuhiro Gemma

    IPC分类号： C12Q1/68 ,  C07H21/00

    CPC分类号： C12Q1/6883 ,  C12Q2600/156

    摘要： There is provided a nucleotide primer set for LAMP amplification, used for detecting genotypes of single-nucleotide polymorphisms G590A, G857A and T341C of a NAT2 gene. There is also provided a nucleotide probe for detection of an amplification product amplified with the primer set according to the present invention. There is also provided a method of detecting the genotypes of NAT2 gene single-nucleotide polymorphisms G590A, G857A and T341C by using the primer set according to the present invention.

    摘要翻译： 提供了LAMP扩增的核苷酸引物组，用于检测NAT2基因的单核苷酸多态性G590A，G857A和T341C的基因型。 还提供了用于检测根据本发明的引物组扩增的扩增产物的核苷酸探针。 还提供了通过使用本发明的引物组来检测NAT2基因单核苷酸多态性G590A，G857A和T341C的基因型的方法。

    NUCLEIC ACID DETECTION DEVICE
    6.
    发明申请
    NUCLEIC ACID DETECTION DEVICE 审中-公开
    核酸检测装置

    公开(公告)号：US20080233634A1

    公开(公告)日：2008-09-25

    申请号：US12048690

    申请日：2008-03-14

    申请人： Jun OKADA ,  Sadato Hongo ,  Keiko Ito ,  Kenji Ooki ,  Nobuhiro Gemma

    发明人： Jun OKADA ,  Sadato Hongo ,  Keiko Ito ,  Kenji Ooki ,  Nobuhiro Gemma

    IPC分类号： C12M1/00

    CPC分类号： G01N35/085

    摘要： A nucleic acid detection device is provided with a closed channel formed of a first channel portion, through which a washing reagent storage section for storing a washing reagent for washing a detection section for nucleic acid detection communicates with the detection section, and a second channel portion through which a pretreatment section for nucleic acid treatment communicates with the detection section. The closed channel is connected with a gas inlet/outlet path for communication with the outside. The gas inlet/outlet path is blocked by a sealing mechanism before nucleic acid detection. In storing a pretreatment reagent and the washing reagent frozen, the gas inlet/outlet path is kept open and connected to the channel. Thus, there is provided a nucleic acid detection device having a structure for preventing leakage of nucleic acid samples to the outside and which can be stored for a long period of time with the reagents therein.

    摘要翻译： 核酸检测装置设置有由第一通道部分形成的封闭通道，用于存储用于清洗用于核酸检测的检测部分的洗涤剂的洗涤剂存储部分与检测部分通信，第二通道部分 用于核酸处理的预处理部分与检测部分通信。 封闭通道与用于与外部连通的气体入口/出口路径连接。 在核酸检测之前，气体入口/出口路径被密封机构堵塞。 在储存预处理试剂和洗涤剂冻结时，气体入口/出口路径保持打开并连接到通道。 因此，提供了一种核酸检测装置，其具有防止核酸样品向外部泄漏的结构，并且可以用其中的试剂长时间储存​​。

    Gene detection method
    9.
    发明授权
    Gene detection method 失效
    基因检测方法

    公开(公告)号：US5972692A

    公开(公告)日：1999-10-26

    申请号：US886161

    申请日：1997-06-30

    申请人： Koji Hashimoto ,  Keiko Ito ,  Yoshio Ishimori

    发明人： Koji Hashimoto ,  Keiko Ito ,  Yoshio Ishimori

    IPC分类号： C12N15/10 ,  C12Q1/68 ,  C12M3/04 ,  C12M1/00

    CPC分类号： C12Q1/6834 ,  B01L7/525 ,  C12N15/1006 ,  C12Q1/6816 ,  C12Q1/6825 ,  G01N27/3276 ,  B01L2300/0645 ,  B01L2300/0819 ,  B01L3/50851 ,  B01L7/54

    摘要： A single stranded nucleic acid probe having a base sequence complementary to the gene to be detected is immobilized onto the surface of an electrode or the tip of an optical fiber, and the nucleic probe is reacted with the gene sample denatured to a single stranded form, and then the nucleic acid probe hybridized with the gene is detected. In this procedure, to the reaction system consisting of the nucleic acid probe and the gene sample, a double stranded nucleic acid recognizing substance capable of binding specifically to the double stranded nucleic acid and being active electrochemically or optically is added. The detection of the nucleic acid probe is conducted by electrochemical or optical determination utilizing the electrode or optical fiber mentioned above. By this method, safer and more convenient detection of the gene is possible at a higher sensitivity even in a reduced time period.

    摘要翻译： 将具有与待检测基因互补的碱基序列的单链核酸探针固定在电极表面或光纤尖端上，使核酸探针与基因样品变性为单链形式， 然后检测与该基因杂交的核酸探针。 在该方法中，对于由核酸探针和基因样品构成的反应体系，添加能够特异性结合双链核酸并且以电化学或光学活性结合的双链核酸识别物质。 通过使用上述电极或光纤的电化学或光学测定来进行核酸探针的检测。 通过这种方法，即使在减少的时间内也可以以更高的灵敏度检测更基础的基因。