公开(公告)号：US20150353608A1

公开(公告)日：2015-12-10

申请号：US14763589

申请日：2013-12-25

Applicant: National Institute of Advanced Industrial Science and Technology

Inventor： Hideki WATANABE ,  Shinya HONDA

IPC: C07K14/00 ,  C07K7/06 ,  C07K7/08

CPC classification number: C07K14/001 ,  C07K7/06 ,  C07K7/08 ,  C07K16/4208 ,  G01N33/6854

Abstract: The invention relates to polypeptides consisting of amino acid sequences represented by the following formulas 1 to 4, which have binding activity to an Fc region of immunoglobulin G and can be favorably used in detecting, purifying, immobilizing or removing an antibody, immunoglobulin G or a protein containing an Fc region of immunoglobulin G, (SEQ ID NO: 1) Y-D-P-x-T-G-T-W-R-S-x-[IL] (1) (SEQ ID NO: 10) R-[QRS]-x-x-[GS]-Y-D-P-R-T-G-T-W-R-S-S-I-A-Y-G-G (2) (SEQ ID NO: 20) G-V-V-R-Q-W-S-G-x-x-x-x-x-x-x-x-R-S-S-I-A-Y-G-G (3) (SEQ ID NO: 23) D-A-A-W-H-L-G-E-L-V-W-A-T-Y-Y-D-P-E-T-G-T-W-x-P-D- W-x-x-M (4) where x represents any amino acid residue; and amino acid residues within the square brackets indicate that any one of the amino acid residues is selected.

Abstract translation: 本发明涉及由下式1〜4表示的氨基酸序列组成的多肽，其具有与免疫球蛋白G的Fc区的结合活性，可有利地用于检测，纯化，固定或除去抗体，免疫球蛋白G或 含有免疫球蛋白G的Fc区的蛋白质（SEQ ID NO：1）YDPxTGTWRSx- [IL]（1）（SEQ ID NO：10）R- [QRS] -xx- [GS] -YDPRTGTWRSSIAYGG（2）（SEQ ID NO： NO：20）GVVRQWSGxxxxxxxxRSSIA-YGG（3）（SEQ ID NO：23）DAAWHLGELVWATYYDPETGT-WxPD-WxxM（4）其中x表示任何氨基酸残基; 方括号内的氨基酸残基表示选择任何一个氨基酸残基。

公开(公告)号：US20190194249A1

公开(公告)日：2019-06-27

申请号：US16329812

申请日：2017-08-31

Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

Inventor： Hideki WATANABE ,  Shinya HONDA

IPC: C07K1/22 ,  C07K7/06 ,  C12N15/62 ,  C07K16/06 ,  C12N7/00

CPC classification number: C07K1/22 ,  C07K7/06 ,  C07K16/00 ,  C07K16/065 ,  C07K2317/32 ,  C07K2317/34 ,  C07K2317/522 ,  C07K2319/70 ,  C12N7/00 ,  C12N15/09 ,  C12N15/62 ,  C12N2799/02

Abstract: The present invention relates to a novel polypeptide having affinity for proteins partially including a CH1-CL domain forming a non-native three-dimensional structure and capable of being suitably used for detecting, immobilizing, or removing these proteins and relates to use of the polypeptide. Specifically, disclosed are a polypeptide consisting of an amino acid sequence represented by any one of the following formulas 1 to 3: (1) P-Q-x-I-x-L-x-[IL]-[NT]-[YW] (SEQ ID NO: 1), (2) Y-D-P-E-T-G-T-W-P-Q-x-I-x-L-x-[IL]-[NT]-[YW] (SEQ ID NO: 4), and (3) P—N-S-G-G-G-G-S-Y-D-P-E-T-G-T-W-P-Q-x-I-x-L-x-[IL]-[NT]-[YW] (SEQ ID NO: 7) (wherein x represents an amino acid residue; and brackets represent any one of the amino acid residues within the brackets), and a method of using the polypeptide to detect, purify, or remove a protein partially including a CH1-CL domain forming a non-native three-dimensional structure.

公开(公告)号：US20160280744A1

公开(公告)日：2016-09-29

申请号：US15025839

申请日：2014-10-01

Applicant: National Institute of Advanced Industrial Science and Technology ,  Daicel Corporation

Inventor： Shinya HONDA ,  Hideki WATANABE ,  Chuya YOSHIDA ,  Yutaka ISOBE ,  Momoko UEDA ,  Yasuto NAKAI

IPC: C07K14/315 ,  C07K16/00 ,  C07K1/22

CPC classification number: C07K14/315 ,  C07K1/22 ,  C07K14/31 ,  C07K16/00 ,  C07K2319/21 ,  C07K2319/30 ,  C07K2319/70 ,  C07K2319/705

Abstract: The purpose of the present invention is to provide a novel protein, which have such excellent pH responsiveness that the antibody binding property to the Fc region of the immunoglobulin in the neutral region is improved and the binding property to the Fc region of the immunoglobulin in the weakly acidic region is much decreased when compared to the wild-type proteins such as protein G and protein A that have affinity for the protein comprising the Fc region of the immunoglobulin G (IgG), and a mutant-type protein (an improved protein) comprising their domain mutant.The present invention relates to a protein constituted by linking plural domains having affinity for a protein comprising the Fc region of the immunoglobulin G (IgG) with linkers, wherein an extended length to the maximum of the linker is 80-240 angstrom.

Abstract translation: 本发明涉及通过将对包含免疫球蛋白G（IgG）的Fc区的蛋白质具有亲和性的多个结合物与连接体连接而构成的蛋白质，其中连接体的最大长度为80-240埃。

公开(公告)号：US20150377899A1

公开(公告)日：2015-12-31

申请号：US14655792

申请日：2013-12-09

Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

Inventor： Shinya HONDA ,  Hideki WATANABE ,  Kazuhiko YAMASAKI

IPC: G01N33/68

CPC classification number: G01N33/6845 ,  C07K7/06 ,  C07K2319/00 ,  C12N15/1034 ,  C12N15/1044 ,  C12N15/1093

Abstract: Disclosed is a molecular library comprising a group of a plurality of molecules, wherein each member of the library is a polypeptide having a randomized sequence moiety and a microprotein moiety. The microprotein is a protein comprising an amino acid sequence of 30 or less amino acid residues having the ability to form a particular conformation by spontaneous folding in a solution and is, for example, chignolin comprising the amino acid sequence represented by SEQ ID NO: 1. Also, disclosed is a method for identifying a novel functional molecule using the library of the present invention.

Abstract translation: 公开了包含多个分子组的分子文库，其中文库的每个成员是具有随机化序列部分和微蛋白部分的多肽。 微蛋白是包含具有30个或更少氨基酸残基的氨基酸序列的蛋白质，其具有通过在溶液中自发折叠形成特定构象的能力，并且是例如包含由SEQ ID NO：1表示的氨基酸序列的chignolin 此外，公开了使用本发明的文库鉴定新型功能分子的方法。