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公开(公告)号：US08772007B2
公开(公告)日：2014-07-08
申请号：US10581014
申请日：2004-12-03
申请人： Neelam S. Amin , Matthew G. Boston , Richard R. Bott , Marguerite A. Cervin , Edward M. Concar , Marc E. Gustwiller , Brian E. Jones , Klaus Liebeton , Gregory S. Miracle , Hiroshi Oh , Ayrookaran J. Poulose , Sandra W. Ramer , Jeffrey J. Scheibel , Walter Weyler , Gregory M. Whited
发明人： Neelam S. Amin , Matthew G. Boston , Richard R. Bott , Marguerite A. Cervin , Edward M. Concar , Marc E. Gustwiller , Brian E. Jones , Klaus Liebeton , Gregory S. Miracle , Hiroshi Oh , Ayrookaran J. Poulose , Sandra W. Ramer , Jeffrey J. Scheibel , Walter Weyler , Gregory M. Whited
CPC分类号： A01N63/00 , C11D3/38636 , C12N9/16 , C12N9/50 , C12N9/52
摘要： The present invention provides methods and compositions comprising at least one perhydrolase enzyme for cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions for generation of peracids. The present invention finds particular use in applications involving cleaning, bleaching and disinfecting.
摘要翻译： 本发明提供包含至少一种用于清洁和其它应用的过水解酶的方法和组合物。 在一些特别优选的实施方案中，本发明提供了用于产生过酸的方法和组合物。 本发明特别用于涉及清洗，漂白和消毒的应用中。
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公开(公告)号：US20080145353A1
公开(公告)日：2008-06-19
申请号：US10581014
申请日：2004-12-03
申请人： Neelam S. Amin , Matthew G. Boston , Richard R. Bott , Marguerite A. Cervin , Edward M. Concar , Marc E. Gustwiller , Brain E. Jones , Klaus Liebeton , Gregory S. Miracle , Hiroshi Oh , Ayrookaran J. Poulose , Sandra W. Ramer , Jeffrey J. Scheibel , Walter Weyler , Gregory M. Whited
发明人： Neelam S. Amin , Matthew G. Boston , Richard R. Bott , Marguerite A. Cervin , Edward M. Concar , Marc E. Gustwiller , Brain E. Jones , Klaus Liebeton , Gregory S. Miracle , Hiroshi Oh , Ayrookaran J. Poulose , Sandra W. Ramer , Jeffrey J. Scheibel , Walter Weyler , Gregory M. Whited
CPC分类号： A01N63/00 , C11D3/38636 , C12N9/16 , C12N9/50 , C12N9/52
摘要： The present invention provides methods and compositions comprising at least one perhydrolase enzyme for cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions for generation of peracids. The present invention finds particular use in applications involving cleaning, bleaching and disinfecting.
摘要翻译： 本发明提供包含至少一种用于清洁和其它应用的过水解酶的方法和组合物。 在一些特别优选的实施方案中，本发明提供了用于产生过酸的方法和组合物。 本发明特别用于涉及清洗，漂白和消毒的应用中。
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公开(公告)号：USRE44648E1
公开(公告)日：2013-12-17
申请号：US13507300
申请日：2012-06-15
申请人： Neelam S. Amin , Richard R. Bott , Marguerite A. Cervin , Ayrookaran J. Poulose , Walter Weyler
发明人： Neelam S. Amin , Richard R. Bott , Marguerite A. Cervin , Ayrookaran J. Poulose , Walter Weyler
摘要： The present invention provides methods and compositions comprising at least one perhydrolase enzyme for cleaning and other applications. In some embodiments, the present invention provides methods and compositions for generation of long chain peracids. Certain embodiments of the present invention find particular use in applications involving cleaning, bleaching and disinfecting.
摘要翻译： 本发明提供包含至少一种用于清洁和其它应用的过水解酶的方法和组合物。 在一些实施方案中，本发明提供用于产生长链过酸的方法和组合物。 本发明的某些实施方案特别用于涉及清洁，漂白和消毒的应用中。
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公开(公告)号：US08476052B2
公开(公告)日：2013-07-02
申请号：US12785250
申请日：2010-05-21
申请人： Neelam S. Amin , Richard R. Bott , Marguerite A. Cervin , Ayrookaran J. Poulose , Walter Weyler
发明人： Neelam S. Amin , Richard R. Bott , Marguerite A. Cervin , Ayrookaran J. Poulose , Walter Weyler
CPC分类号： C12N9/16 , C11D3/38636
摘要： The present invention provides methods and compositions comprising at least one perhydrolase enzyme for cleaning and other applications. In some embodiments, the present invention provides methods and compositions for generation of long chain peracids. Certain embodiments of the present invention find particular use in applications involving cleaning, bleaching and disinfecting.
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公开(公告)号：US07754460B2
公开(公告)日：2010-07-13
申请号：US11595537
申请日：2006-11-09
申请人： Neelam S. Amin , Richard R. Bott , Marguerite A. Cervin , Ayrookaran J. Poulose , Walter Weyler
发明人： Neelam S. Amin , Richard R. Bott , Marguerite A. Cervin , Ayrookaran J. Poulose , Walter Weyler
CPC分类号： C12N9/16 , C11D3/38636
摘要： The present invention provides methods and compositions comprising at least one perhydrolase enzyme for cleaning and other applications. In some embodiments, the present invention provides methods and compositions for generation of long chain peracids. Certain embodiments of the present invention find particular use in applications involving cleaning, bleaching and disinfecting.
摘要翻译： 本发明提供包含至少一种用于清洁和其它应用的过水解酶的方法和组合物。 在一些实施方案中，本发明提供用于产生长链过酸的方法和组合物。 本发明的某些实施方案特别用于涉及清洁，漂白和消毒的应用中。
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6.发明授权公开(公告)号：US08916370B2
公开(公告)日：2014-12-23
申请号：US13436612
申请日：2012-03-30
申请人： Richard R. Bott , Marguerite A. Cervin , James T. Kellis, Jr. , Joseph C. McAuliffe , Andrei Miasnikov , Caroline M. Peres , Christopher Lee Rife , Derek H. Wells , Walter Weyler , Gregory M. Whited
发明人： Richard R. Bott , Marguerite A. Cervin , James T. Kellis, Jr. , Joseph C. McAuliffe , Andrei Miasnikov , Caroline M. Peres , Christopher Lee Rife , Derek H. Wells , Walter Weyler , Gregory M. Whited
IPC分类号： C12N9/88 , C12P21/06 , C12N15/00 , C12P5/02 , C12N1/20 , C12N1/00 , C07H21/04 , C07K1/00 , C12P5/00
CPC分类号： C12N9/88 , C12P5/007 , C12Y402/03027
摘要： The present invention provides methods and compositions comprising at least one isoprene synthase enzyme with improved catalytic activity and/or solubility. In particular, the present invention provides variant plant isoprene synthases for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.
摘要翻译： 本发明提供包含至少一种具有改善的催化活性和/或溶解度的异戊二烯合酶的方法和组合物。 特别地，本发明提供用于在微生物宿主细胞中增加异戊二烯生产的变体植物异戊二烯合酶。 本发明的生物合成生产的异戊二烯可用于制造橡胶和弹性体。
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7.发明授权公开(公告)号：US08173410B2
公开(公告)日：2012-05-08
申请号：US12429143
申请日：2009-04-23
申请人： Richard R. Bott , Marguerite A. Cervin , James T. Kellis, Jr. , Joseph C. McAuliffe , Andrei Miasnikov , Caroline M. Peres , Christopher Lee Rife , Derek H. Wells , Walter Weyler , Gregory M. Whited
发明人： Richard R. Bott , Marguerite A. Cervin , James T. Kellis, Jr. , Joseph C. McAuliffe , Andrei Miasnikov , Caroline M. Peres , Christopher Lee Rife , Derek H. Wells , Walter Weyler , Gregory M. Whited
CPC分类号： C12N9/88 , C12P5/007 , C12Y402/03027
摘要： The present invention provides methods and compositions comprising at least one isoprene synthase enzyme with improved catalytic activity and/or solubility. In particular, the present invention provides variant plant isoprene synthases for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.
摘要翻译： 本发明提供包含至少一种具有改善的催化活性和/或溶解度的异戊二烯合酶的方法和组合物。 特别地，本发明提供用于在微生物宿主细胞中增加异戊二烯生产的变体植物异戊二烯合酶。 本发明的生物合成生产的异戊二烯可用于制造橡胶和弹性体。
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8.发明申请公开(公告)号：US20100003716A1
公开(公告)日：2010-01-07
申请号：US12429143
申请日：2009-04-23
申请人： Marguerite A. Cervin , Gregory M. Whited , Andrei Miasnikov , Caroline M. Peres , Walter Weyler , Derek H. Wells , Richard R. Bott
发明人： Marguerite A. Cervin , Gregory M. Whited , Andrei Miasnikov , Caroline M. Peres , Walter Weyler , Derek H. Wells , Richard R. Bott
CPC分类号： C12N9/88 , C12P5/007 , C12Y402/03027
摘要： The present invention provides methods and compositions comprising at least one isoprene synthase enzyme with improved catalytic activity and/or solubility. In particular, the present invention provides variant plant isoprene synthases for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.
摘要翻译： 本发明提供包含至少一种具有改善的催化活性和/或溶解度的异戊二烯合酶的方法和组合物。 特别地，本发明提供用于在微生物宿主细胞中增加异戊二烯生产的变体植物异戊二烯合酶。 本发明的生物合成生产的异戊二烯可用于制造橡胶和弹性体。
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公开(公告)号：US20090011489A1
公开(公告)日：2009-01-08
申请号：US11929929
申请日：2007-10-30
CPC分类号： C12N9/54 , C11D3/386 , C11D3/38609 , C12Y304/21062
摘要： Novel enzyme variants including protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease, Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue correspond to positions 27, 45, 170, 181, 251 and 271 of Bacillus amyloliquefaciens subtilisin. Additional variants comprising at least one additional substitution at a position selected from 1, 14, 49, 61, 87, 100, 102, 118, 128, 204 and 258 of Bacillus amyloliquefaciens subtilisin are also described.
摘要翻译： 公开了包含衍生自天然或重组非人蛋白酶的DNA序列的蛋白酶变体的新型酶变体。 变体蛋白酶一般通过体外修饰编码天然存在或重组蛋白酶的前体DNA序列获得，以产生前体蛋白酶的氨基酸序列中多个氨基酸残基的取代。这种变体蛋白酶具有 与前体蛋白酶不同的性质，例如改变的洗涤性能。 取代的氨基酸残基对应于解淀粉芽孢杆菌枯草杆菌蛋白酶的位置27,45,170,181,251和271。 还描述了包含在解淀粉芽孢杆菌枯草杆菌蛋白酶的1,4,14,69,61,87,100,102,118,128,204和258的位置上的至少一个另外的取代的其它变体。
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公开(公告)号：US20110091959A1
公开(公告)日：2011-04-21
申请号：US12776940
申请日：2010-05-10
CPC分类号： C12N9/54 , C11D3/386 , C11D3/38609 , C12Y304/21062
摘要： Novel enzyme variants including protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue correspond to positions 27, 45, 170, 181, 251 and 271 of Bacillus amyloliquefaciens subtilisin. Additional variants comprising at least one additional substitution at a position selected from 1, 14, 49, 61, 87, 100, 102, 118, 128, 204 and 258 of Bacillus amyloliquefaciens subtilisin are also described.
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