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    Laser Heating of Aqueous Samples on a Micro-Optical-Electro-Mechanical System
    1.
    发明申请
    Laser Heating of Aqueous Samples on a Micro-Optical-Electro-Mechanical System 有权
    在微光机电系统上的水样品的激光加热

    公开(公告)号：US20090261086A1

    公开(公告)日：2009-10-22

    申请号：US12331487

    申请日：2008-12-10

    申请人： Neil Reginald Beer ,  Ian Kennedy

    发明人： Neil Reginald Beer ,  Ian Kennedy

    IPC分类号： H05B7/18

    CPC分类号： G01N25/00 ,  H05B3/0033

    摘要： A system of heating a sample on a microchip includes the steps of providing a microchannel flow channel in the microchip; positioning the sample within the microchannel flow channel, providing a laser that directs a laser beam onto the sample for heating the sample; providing the microchannel flow channel with a wall section that receives the laser beam and enables the laser beam to pass through wall section of the microchannel flow channel without being appreciably heated by the laser beam; and providing a carrier fluid in the microchannel flow channel that moves the sample in the microchannel flow channel wherein the carrier fluid is not appreciably heated by the laser beam.

    摘要翻译： 在微芯片上加热样品的系统包括以下步骤：在微芯片中提供微通道流动通道; 将样品定位在微通道流动通道内，提供将激光束引导到样品上用于加热样品的激光; 为微通道流动通道提供接收激光束的壁部分，并且使得激光束能够通过微通道流动通道的壁部分而不被激光束明显加热; 以及在所述微通道流动通道中提供载体流体，所述载体流体使所述样品在所述微通道流动通道中移动，其中所述载体流体不被所述激光束明显加热。

    Solid state rapid thermocycling
    3.
    发明授权
    Solid state rapid thermocycling 有权
    固态快速热循环

    公开(公告)号：US08720209B1

    公开(公告)日：2014-05-13

    申请号：US13267777

    申请日：2011-10-06

    申请人： Neil Reginald Beer ,  Christopher Spadaccini

    发明人： Neil Reginald Beer ,  Christopher Spadaccini

    IPC分类号： B01L3/00

    CPC分类号： F24H1/00 ,  B01L7/52 ,  B01L7/54 ,  B01L2200/0689 ,  B01L2200/12 ,  B01L2300/0829 ,  B01L2300/1805 ,  B01L2300/1822 ,  B01L2300/1827 ,  C12M29/00 ,  C12M41/12 ,  C12M41/18 ,  C12M41/48 ,  F24H1/10

    摘要： The rapid thermal cycling of a material is targeted. A solid state heat exchanger with a first well and second well is coupled to a power module. A thermoelectric element is coupled to the first well, the second well, and the power module, is configured to transfer thermal energy from the first well to the second well when current from the power module flows through the thermoelectric element in a first direction, and is configured to transfer thermal energy from the second well to the first well when current from the power module flows through the thermoelectric element in a second direction. A controller may be coupled to the thermoelectric elements, and may switch the direction of current flowing through the thermoelectric element in response to a determination by sensors coupled to the wells that the amount of thermal energy in the wells falls below or exceeds a pre-determined threshold.

    摘要翻译： 材料的快速热循环是目标。 具有第一阱和第二阱的固态热交换器耦合到功率模块。 热电元件耦合到第一阱，第二阱和功率模块被配置为当来自功率模块的电流沿着第一方向流过热电元件时将热能从第一阱传递到第二阱，以及 配置成当来自功率模块的电流沿第二方向流过热电元件时，将热能从第二阱传递到第一阱。 控制器可以耦合到热电元件，并且可以响应于耦合到井的传感器的确定来切换流过热电元件的电流的方向，即孔中的热能量低于或超过预定值 阈。

    Chip-Based Sequencing Nucleic Acids
    6.
    发明申请
    Chip-Based Sequencing Nucleic Acids 有权
    基于芯片的测序核酸

    公开(公告)号：US20100184020A1

    公开(公告)日：2010-07-22

    申请号：US11965585

    申请日：2007-12-27

    申请人： Neil Reginald Beer

    发明人： Neil Reginald Beer

    IPC分类号： C12Q1/68 ,  C12M1/34

    CPC分类号： C12Q1/6834 ,  B01L3/502761 ,  B01L3/502784 ,  B01L7/52 ,  B01L2200/0668 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/1827 ,  B01L2300/1861 ,  B01L2400/043 ,  B01L2400/0487 ,  C12Q1/6869 ,  C12Q2563/143 ,  C12Q2563/155 ,  C12Q2565/629

    摘要： A system for fast DNA sequencing by amplification of genetic material within microreactors, denaturing, demulsifying, and then sequencing the material, while retaining it in a PCR/sequencing zone by a magnetic field. One embodiment includes sequencing nucleic acids on a microchip that includes a microchannel flow channel in the microchip. The nucleic acids are isolated and hybridized to magnetic nanoparticles or to magnetic polystyrene-coated beads. Microreactor droplets are formed in the microchannel flow channel. The microreactor droplets containing the nucleic acids and the magnetic nanoparticles are retained in a magnetic trap in the microchannel flow channel and sequenced.

    摘要翻译： 通过在微反应器中扩增遗传物质进行快速DNA测序的系统，使其变性，破乳，然后对材料进行测序，同时通过磁场将其保留在PCR /测序区域。 一个实施方案包括在微芯片上测序核酸，其包括微芯片中的微通道流动通道。 核酸被分离并与磁性纳米颗粒或磁性聚苯乙烯涂覆的珠子杂交。 在微通道流动通道中形成微反应器液滴。 含有核酸和磁性纳米颗粒的微反应器液滴保留在微通道流动通道中的磁阱中并进行测序。

    Methods and compositions for rapid thermal cycling
    8.
    发明授权
    Methods and compositions for rapid thermal cycling 有权
    快速热循环的方法和组成

    公开(公告)号：US09170028B1

    公开(公告)日：2015-10-27

    申请号：US13267767

    申请日：2011-10-06

    申请人： Neil Reginald Beer ,  William J. Benett ,  James M. Frank ,  Joshua R. Deotte ,  Christopher Spadaccini

    发明人： Neil Reginald Beer ,  William J. Benett ,  James M. Frank ,  Joshua R. Deotte ,  Christopher Spadaccini

    IPC分类号： F24H1/00 ,  F24H1/10 ,  H05B3/78

    CPC分类号： F24H1/00 ,  B01L7/52 ,  B01L7/54 ,  B01L2200/0689 ,  B01L2200/12 ,  B01L2300/0829 ,  B01L2300/1805 ,  B01L2300/1822 ,  B01L2300/1827 ,  C12M29/00 ,  C12M41/12 ,  C12M41/18 ,  C12M41/48 ,  F24H1/10

    摘要： The rapid thermal cycling of a material is targeted. A microfluidic heat exchanger with an internal porous medium is coupled to tanks containing cold fluid and hot fluid. Fluid flows alternately from the cold tank and the hot tank into the porous medium, cooling and heating samples contained in the microfluidic heat exchanger's sample wells. A valve may be coupled to the tanks and a pump, and switching the position of the valve may switch the source and direction of fluid flowing through the porous medium. A controller may control the switching of valve positions based on the temperature of the samples and determined temperature thresholds. A sample tray for containing samples to be thermally cycled may be used in conjunction with the thermal cycling system. A surface or internal electrical heater may aid in heating the samples, or may replace the necessity for the hot tank.

    摘要翻译： 材料的快速热循环是目标。 具有内部多孔介质的微流体热交换器耦合到容纳冷流体和热流体的罐。 流体从冷箱和热罐交替地流入多孔介质，冷却和加热微流体换热器样品孔中包含的样品。 阀可以联接到罐和泵，并且切换阀的位置可以切换流过多孔介质的流体的源和方向。 控制器可以基于样品的温度和确定的温度阈值来控制阀位置的切换。 用于包含热循环样品的样品盘可以与热循环系统一起使用。 表面或内部电加热器可能有助于加热样品，也可能取代热水箱的必要性。

    Chip-based sequencing nucleic acids
    9.
    发明授权
    Chip-based sequencing nucleic acids 有权
    基于芯片的测序核酸

    公开(公告)号：US08815576B2

    公开(公告)日：2014-08-26

    申请号：US11965585

    申请日：2007-12-27

    申请人： Neil Reginald Beer

    发明人： Neil Reginald Beer

    IPC分类号： C12Q1/68 ,  C12M1/00 ,  C12M3/00 ,  C12M1/34

    CPC分类号： C12Q1/6834 ,  B01L3/502761 ,  B01L3/502784 ,  B01L7/52 ,  B01L2200/0668 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/1827 ,  B01L2300/1861 ,  B01L2400/043 ,  B01L2400/0487 ,  C12Q1/6869 ,  C12Q2563/143 ,  C12Q2563/155 ,  C12Q2565/629

    摘要： A system for fast DNA sequencing by amplification of genetic material within microreactors, denaturing, demulsifying, and then sequencing the material, while retaining it in a PCR/sequencing zone by a magnetic field. One embodiment includes sequencing nucleic acids on a microchip that includes a microchannel flow channel in the microchip. The nucleic acids are isolated and hybridized to magnetic nanoparticles or to magnetic polystyrene-coated beads. Microreactor droplets are formed in the microchannel flow channel. The microreactor droplets containing the nucleic acids and the magnetic nanoparticles are retained in a magnetic trap in the microchannel flow channel and sequenced.

    摘要翻译： 通过在微反应器中扩增遗传物质进行快速DNA测序的系统，使其变性，破乳，然后对材料进行测序，同时通过磁场将其保留在PCR /测序区域。 一个实施方案包括在微芯片上测序核酸，其包括微芯片中的微通道流动通道。 核酸被分离并与磁性纳米颗粒或磁性聚苯乙烯涂覆的珠子杂交。 在微通道流动通道中形成微反应器液滴。 含有核酸和磁性纳米颗粒的微反应器液滴保留在微通道流动通道中的磁阱中并进行测序。

    Multi-well sample plate cover penetration system
    10.
    发明授权
    Multi-well sample plate cover penetration system 有权
    多孔样品板盖渗透系统

    公开(公告)号：US08084005B2

    公开(公告)日：2011-12-27

    申请号：US11342361

    申请日：2006-01-26

    申请人： Neil Reginald Beer

    发明人： Neil Reginald Beer

    IPC分类号： B01L3/00

    CPC分类号： B01L3/50853 ,  B01L2300/044 ,  B01L2300/0829 ,  G01N35/1065 ,  G01N35/1079 ,  G01N2035/0405 ,  Y10T83/0481 ,  Y10T83/222 ,  Y10T83/869

    摘要： An apparatus for penetrating a cover over a multi-well sample plate containing at least one individual sample well includes a cutting head, a cutter extending from the cutting head, and a robot. The cutting head is connected to the robot wherein the robot moves the cutting head and cutter so that the cutter penetrates the cover over the multi-well sample plate providing access to the individual sample well. When the cutting head is moved downward the foil is pierced by the cutter that splits, opens, and folds the foil inward toward the well. The well is then open for sample aspiration but has been protected from cross contamination.

    摘要翻译： 一种用于在包含至少一个单个样品井的多孔样品板上穿透盖的装置包括切割头，从切割头延伸的切割器和机器人。 切割头连接到机器人，其中机器人移动切割头和切割器，使得切割器穿过多孔样品板上的盖，从而提供对单个样品孔的通路。 当切割头向下移动时，箔片被切割器刺穿，该切割器分裂，打开并将箔片向内朝向孔折叠。 然后将孔打开以供样品抽吸，但已被保护免受交叉污染。