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1.发明授权Anti-human calcitonin monoclonal antibodies and an immunoassay utilizing said antibodies 失效抗人降钙素单克隆抗体和利用所述抗体的免疫测定
公开(公告)号：US6133427A
公开(公告)日：2000-10-17
申请号：US997479
申请日：1997-12-23
IPC分类号： G01N33/53 , A61K39/395 , C07H21/04 , C07K16/26 , C12N5/10 , C12N15/00 , C12N15/02 , C12P21/08 , C12R1/91 , G01N33/574 , G01N33/577
CPC分类号： C07K16/26 , C07K2317/34
摘要： Monoclonal antibodies having a high affinity for human calcitonin, particularly monoclonal antibodies suitable for a sandwich immunoassay are disclosed. Also disclosed are hybridomas producing said monoclonal antibodies and a sandwich immunoassay utilizing said antibodies for determining human calcitonin in blood.
摘要翻译： 公开了对人类降钙素具有高亲和力的单克隆抗体，特别是适用于夹心免疫测定法的单克隆抗体。 还公开了产生所述单克隆抗体的杂交瘤和利用所述抗体测定血液中人降钙素的夹心免疫测定。
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公开(公告)号：US06277964B1
公开(公告)日：2001-08-21
申请号：US09265872
申请日：1999-03-11
申请人： Nobuhiko Yamashita , Kazunobu Miura , Naoya Ichimura , Chiwa Kataoka , Junko Sakaki , Yutaka Ohtani , Hirohisa Kitagawa
发明人： Nobuhiko Yamashita , Kazunobu Miura , Naoya Ichimura , Chiwa Kataoka , Junko Sakaki , Yutaka Ohtani , Hirohisa Kitagawa
IPC分类号： C07K1644
CPC分类号： C07K16/44 , G01N33/5308 , Y10S530/808 , Y10S530/809
摘要： The present invention provides monoclonal antibodies suitable for monitoring polycyclic aromatic hydrocarbons (PAHs) by an immunoasay, hybridoma cell lines producing said antibodies, and an immunoassay for analyzing PAHs in a sample using said monoclonal antibodies. In addition, the present invention provides PAH conjugates useful as an immunogen in preparing said antibodies and as a standard substance in a competitive assay.
摘要翻译： 本发明提供了适用于通过免疫测定监测多环芳烃（PAH）的单克隆抗体，产生所述抗体的杂交瘤细胞系以及使用所述单克隆抗体分析样品中PAH的免疫测定法。 此外，本发明提供了在竞争性测定中用作制备所述抗体和作为标准物质的免疫原的PAH缀合物。
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公开(公告)号：US5439811A
公开(公告)日：1995-08-08
申请号：US212708
申请日：1994-03-14
摘要： A method for preparing aminoacyl- and misaminoacyl-tRNAs utilizing a specific aminoacyl-tRNA hydrolase is provided. According to the present method, aminoacyl-tRNAs consisting of a tRNA and a corresponding amino acid, or misaminoacyl-tRNAs consisting of a tRNA and a non-corresponding amino acid or an amino acid derivative, can be prepared. In a specific embodiment, L-tyrosyl-tRNA.sup.Tyr is prepared using aminoacyl-tRNA hydrolase from Fusarium culmorum or Artemia salina. In another embodiment a misacylated tRNA, L-phenylalanyl-tRNA.sup.Tyr, is prepared using the aminoacyl-tRNA hydrolase.
摘要翻译： 提供了利用特异氨基-tRNA水解酶制备氨酰基和错氨基酰基-tRNA的方法。 根据本方法，可以制备由tRNA和相应的氨基酸组成的氨酰-tRNA或由tRNA和非对应的氨基酸或氨基酸衍生物组成的错氨基酰基-tRNA。 在一个具体实施方案中，使用来自镰孢镰刀菌或卤虫盐酸盐的氨基酰-tRNA水解酶制备L-酪氨酰-tRNATyr。 在另一个实施方案中，使用氨酰-tRNA水解酶制备错酰基化的tRNA，L-苯丙氨酰-tRNATyr。
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4.发明授权Monoclonal antibody specifically recognizing N-terminal portion of human calcitonin 失效特异性识别人降钙素的N末端部分的单克隆抗体
公开(公告)号：US5330909A
公开(公告)日：1994-07-19
申请号：US958083
申请日：1992-10-08
IPC分类号： C12N5/10 , C07K16/26 , C12N15/02 , C12P21/08 , C12R1/91 , G01N33/53 , G01N33/577 , G01N33/74 , C07K15/28 , C12N5/20
CPC分类号： C07K16/26 , C07K2317/34
摘要： A monoclonal antibody specifically recognizing N-terminal portion of human calcitonin (hCT), a hybridoma producing said monoclonal antibody, a process for preparing said monoclonal antibody, and a method for detecting hCT which comprises the use of said monoclonal antibody are provided.The above monoclonal antibody permits detection of hCT with high sensitivity.
摘要翻译： 提供特异性识别人降钙素（hCT）的N末端部分的单克隆抗体，产生所述单克隆抗体的杂交瘤，制备所述单克隆抗体的方法，以及包含使用所述单克隆抗体的检测hCT的方法。 上述单克隆抗体允许以高灵敏度检测hCT。
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公开(公告)号：US5556768A
公开(公告)日：1996-09-17
申请号：US266750
申请日：1994-06-28
申请人： Nobuhiko Yamashita
发明人： Nobuhiko Yamashita
IPC分类号： C12P21/02
CPC分类号： C12P21/02
摘要： A novel method for synthesizing peptides and proteins having a desired amino acid sequence by utilizing an aminoacyl-tRNA and misaminoacyl-tRNA as well as an artificial mRNA in a system for in vitro protein synthesis is provided. According to the present method, desired peptides and proteins can be synthesized using naturally-occurring and non naturally-occurring amino acids. Accordingly, the present invention provides a fundamental technique which is important in the fields of various industries and various fundamental researches such as biochemistry, molecular biology and protein engineering.
摘要翻译： 提供了一种通过利用氨酰-tRNA和错氨基酰-tRNA以及体外蛋白质合成系统中的人工mRNA合成具有所需氨基酸序列的肽和蛋白质的新方法。 根据本方法，可以使用天然存在和非天然存在的氨基酸合成所需的肽和蛋白质。 因此，本发明提供了在各行业和生物化学，分子生物学和蛋白质工程等各种基础研究领域中重要的基础技术。
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