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8 条记录 (耗时 0.029 秒)

    Method for preparing aminoacyl- and misaminoacyl-tRNA
    3.
    发明授权
    Method for preparing aminoacyl- and misaminoacyl-tRNA 失效
    制备氨酰基 - 和misaminoacyl-tRNA的方法

    公开(公告)号：US5439811A

    公开(公告)日：1995-08-08

    申请号：US212708

    申请日：1994-03-14

    申请人： Nobuhiko Yamashita ,  Junichi Tohyama ,  Chiwa Kataoka ,  Kazunobu Miura

    发明人： Nobuhiko Yamashita ,  Junichi Tohyama ,  Chiwa Kataoka ,  Kazunobu Miura

    IPC分类号： C12N9/22 ,  C12N9/16 ,  C12N15/09 ,  C12P19/34 ,  C12R1/77 ,  C07H21/02

    CPC分类号： C12N9/16 ,  C12P19/34

    摘要： A method for preparing aminoacyl- and misaminoacyl-tRNAs utilizing a specific aminoacyl-tRNA hydrolase is provided. According to the present method, aminoacyl-tRNAs consisting of a tRNA and a corresponding amino acid, or misaminoacyl-tRNAs consisting of a tRNA and a non-corresponding amino acid or an amino acid derivative, can be prepared. In a specific embodiment, L-tyrosyl-tRNA.sup.Tyr is prepared using aminoacyl-tRNA hydrolase from Fusarium culmorum or Artemia salina. In another embodiment a misacylated tRNA, L-phenylalanyl-tRNA.sup.Tyr, is prepared using the aminoacyl-tRNA hydrolase.

    摘要翻译： 提供了利用特异氨基-tRNA水解酶制备氨酰基和错氨基酰基-tRNA的方法。 根据本方法，可以制备由tRNA和相应的氨基酸组成的氨酰-tRNA或由tRNA和非对应的氨基酸或氨基酸衍生物组成的错氨基酰基-tRNA。 在一个具体实施方案中，使用来自镰孢镰刀菌或卤虫盐酸盐的氨基酰-tRNA水解酶制备L-酪氨酰-tRNATyr。 在另一个实施方案中，使用氨酰-tRNA水解酶制备错酰基化的tRNA，L-苯丙氨酰-tRNATyr。

    Recombinant antibody recognizing dioxin and gene encoding the antibody
    4.
    发明授权
    Recombinant antibody recognizing dioxin and gene encoding the antibody 有权
    识别二恶英的重组抗体和编码抗体的基因

    公开(公告)号：US07381798B2

    公开(公告)日：2008-06-03

    申请号：US10550951

    申请日：2004-03-26

    申请人： Kazuyuki Sawadaishi ,  Keiichi Higano ,  Chiwa Kataoka

    发明人： Kazuyuki Sawadaishi ,  Keiichi Higano ,  Chiwa Kataoka

    IPC分类号： G01N33/53 ,  C12P21/04 ,  C12P21/08 ,  C12N1/20 ,  C12N5/06 ,  C07K16/00 ,  C07H21/04

    CPC分类号： G01N33/5308 ,  C07K16/44

    摘要： The present invention relates to a novel recombinant antibody having a binding activity to 2,3,4,7,8-pentachlorodibenzofuran (2,3,4,7,8-PeCDF), a gene encoding its amino acid sequence, a vector incorporating said gene, a transformant transformed with said vector, a process for preparing said recombinant antibody, and methods for immunologically capturing and determining 2,3,4,7,8-PeCDF using said recombinant antibody. Using the recombinant antibody according to the present invention, it is possible to capture and determine dioxins, in particular, 2,3,4,7,8-PeCDF by an immunological technique in a rapid, convenient, and highly sensitive manner.

    摘要翻译： 本发明涉及对2,3,4,7,8-五氯二苯并呋喃（2,3,4,7,8-PeCDF）具有结合活性的新型重组抗体，编码其氨基酸序列的基因， 所述基因，用所述载体转化的转化体，制备所述重组抗体的方法，以及使用所述重组抗体免疫捕获和测定2,3,4,7,8-PeCDF的方法。 使用根据本发明的重组抗体，可以通过免疫学技术以快速，方便和高度敏感的方式捕获并测定二恶英，特别是2,3,4,7,8-PeCDF。

    Method and Apparatus for Screening and Assaying Environmental Sample
    6.
    发明申请
    Method and Apparatus for Screening and Assaying Environmental Sample 审中-公开
    筛选和分析环境样品的方法和装置

    公开(公告)号：US20090292477A1

    公开(公告)日：2009-11-26

    申请号：US11922123

    申请日：2005-06-14

    申请人： Takashi Matsuki ,  Kazuyuki Sawadaishi ,  Chiwa Kataoka ,  Yoko Takagi

    发明人： Takashi Matsuki ,  Kazuyuki Sawadaishi ,  Chiwa Kataoka ,  Yoko Takagi

    IPC分类号： G01N33/48 ,  G06F19/00

    CPC分类号： G01N33/5308 ,  G01N21/05 ,  G01N21/6428 ,  G01N33/54366 ,  G01N2021/7763 ,  G01N2430/40

    摘要： A screening measurement technology enabling a prompt determination about whether or not further confirmation is required by another measuring method, for an environmental sample, such as a sample containing dioxins, is provided. A sample solution in which a known amount of antibodies are mixed, is poured into a measurement cell (101) where an antigen derivative (107) that acquires antibodies of a subject substance comprising an antigen, is arranged in a flow channel (106) for the sample solution; whether or not a measurement result is within a pre-determined range including a reference value of the subject substance is determined based upon time-series signals of the measurement result; and the measurement result is transmitted.

    摘要翻译： 提供了一种筛选测量技术，能够对环境样品（例如含有二恶英的样品）进行另一种测量方法是否需要进一步确认是否需要进一步确定。 将其中混合已知量的抗体的样品溶液倒入测量细胞（101）中，其中获得包含抗原的受试物质的抗体的抗原衍生物（107）被布置在流路（106）中，用于 样品溶液; 基于测量结果的时间序列信号确定测量结果是否在包括被摄体的参考值的预定范围内; 并传送测量结果。

    Recombinant antibody recognizing dioxin and gene encoding the antibody
    7.
    发明申请
    Recombinant antibody recognizing dioxin and gene encoding the antibody 有权
    识别二恶英的重组抗体和编码抗体的基因

    公开(公告)号：US20060269977A1

    公开(公告)日：2006-11-30

    申请号：US10550951

    申请日：2004-03-26

    申请人： Kazuyuki Sawadaishi ,  Keiichi Higano ,  Chiwa Kataoka

    发明人： Kazuyuki Sawadaishi ,  Keiichi Higano ,  Chiwa Kataoka

    IPC分类号： G01N33/53 ,  C07H21/04 ,  C12P21/06 ,  C07K16/18 ,  C12N5/06

    CPC分类号： G01N33/5308 ,  C07K16/44

    摘要： The present invention relates to a novel recombinant antibody having a binding activity to 2,3,4,7,8-pentachlorodibenzofuran (2,3,4,7,8-PeCDF), a gene encoding its amino acid sequence, a vector incorporating said gene, a transformant transformed with said vector, a process for preparing said recombinant antibody, and methods for immunologically capturing and determining 2,3,4,7,8-PeCDF using said recombinant antibody. Using the recombinant antibody according to the present invention, it is possible to capture and determine dioxins, in particular, 2,3,4,7,8-PeCDF by an immunological technique in a rapid, convenient, and highly sensitive manner.

    摘要翻译： 本发明涉及对2,3,4,7,8-五氯二苯并呋喃（2,3,4,7,8-PeCDF）具有结合活性的新型重组抗体，编码其氨基酸序列的基因， 所述基因，用所述载体转化的转化体，制备所述重组抗体的方法，以及使用所述重组抗体免疫捕获和测定2,3,4,7,8-PeCDF的方法。 使用根据本发明的重组抗体，可以通过免疫学技术以快速，方便和高度敏感的方式捕获并测定二恶英，特别是2,3,4,7,8-PeCDF。

    Anti-dioxins monoclonal antibody suitable for assaying dioxins in environment and hybridoma producing the same
    8.
    发明授权
    Anti-dioxins monoclonal antibody suitable for assaying dioxins in environment and hybridoma producing the same 失效
    抗二恶英单克隆抗体适用于测定环境中的二恶英和产生相同的杂交瘤

    公开(公告)号：US07038021B2

    公开(公告)日：2006-05-02

    申请号：US10885229

    申请日：2004-07-07

    申请人： Yoko Takagi ,  Kazuyuki Sawadaishi ,  Chiwa Kataoka

    发明人： Yoko Takagi ,  Kazuyuki Sawadaishi ,  Chiwa Kataoka

    IPC分类号： C07K16/00 ,  C12N5/20 ,  G01N33/53 ,  G01N33/531 ,  A01N43/28

    CPC分类号： G01N33/5308 ,  C07K16/44 ,  Y10S435/961 ,  Y10S436/815 ,  Y10S530/809

    摘要： The object of present invention is to provide an immunoassay for dioxins which can rapidly and simply afford measured values having a good correlation with analytical values of dioxins by the official method (GC/MS method). The above object is achieved by using the monoclonal antibody of the present invention having not only a reactivity with the indicator isomer among 17 kinds of PCDDs and PCDFs each having a predetermined WHO-TEF value, but also a high cross-reactivity with several kinds of dioxin isomers having five or six chlorine atoms which contribute largely to a TEQ value, and also having a stable reactivity with the antigens in a measuring solvent.

    摘要翻译： 本发明的目的是提供二恶英的免疫测定，其可以通过官方方法（GC / MS方法）快速且简单地提供与二恶英的分析值具有良好相关性的测量值。 上述目的是通过使用本发明的单克隆抗体，其在具有预定的WHO-TEF值的17种PCDD和PCDF中不仅具有与指示异构体的反应性，而且与几种 具有五个或六个氯原子的二恶英异构体，其主要贡献于TEQ值，并且在测量溶剂中也与抗原具有稳定的反应性。